1000-0139

(E)-N'-(2-methoxyphenyl)but-2-enediamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_897
Screen concentration 31.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 6151020
SMILES COC1=CC=CC=C1NC(=O)C=CC(=O)N
Standardized SMILES COc1ccccc1NC(=O)C=CC(=O)N
Molecular weight 220.2246
ALogP 0.55
H-bond donor count 2
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.65
% growth inhibition (Hom. pool) 9.93


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6151020
Download HIP data (tab-delimited text)  (excel)
Gene:ASA1(YPR085C)|FD-Score:6.2|P-value:2.76E-10|Clearance:0.51||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CCT4(YDL143W)|FD-Score:3.5|P-value:2.35E-4|Clearance:0.22||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC12(YHR107C)|FD-Score:3.26|P-value:5.58E-4|Clearance:0.02||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:DBP2(YNL112W)|FD-Score:3.61|P-value:1.52E-4|Clearance:0.02||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:GPN2(YOR262W)|FD-Score:4.6|P-value:2.10E-6|Clearance:0.69||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:GPN3(YLR243W_p)|FD-Score:8.48|P-value:1.13E-17|Clearance:0.84||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p Gene:PUP1(YOR157C)|FD-Score:5.03|P-value:2.47E-7|Clearance:0.43||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RBA50(YDR527W)|FD-Score:5.69|P-value:6.25E-9|Clearance:0.66||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RET1(YOR207C)|FD-Score:11.7|P-value:3.92E-32|Clearance:3.26||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPL10(YLR075W)|FD-Score:-3.6|P-value:1.58E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPT6(YGL048C)|FD-Score:3.24|P-value:6.08E-4|Clearance:0.49||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RTP1(YMR185W_p)|FD-Score:6.31|P-value:1.40E-10|Clearance:0.11||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SCC2(YDR180W)|FD-Score:-3.38|P-value:3.62E-4|Clearance:0||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:SEC26(YDR238C)|FD-Score:3.6|P-value:1.62E-4|Clearance:0.1||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SLD3(YGL113W)|FD-Score:-3.38|P-value:3.64E-4|Clearance:0||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPC42(YKL042W)|FD-Score:3.28|P-value:5.25E-4|Clearance:0.02||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:TAP42(YMR028W)|FD-Score:-3.95|P-value:3.99E-5|Clearance:0||SGD DESC:Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits Gene:TIF11(YMR260C)|FD-Score:3.92|P-value:4.51E-5|Clearance:0.01||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:TIF35(YDR429C)|FD-Score:-3.47|P-value:2.62E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOM22(YNL131W)|FD-Score:3.6|P-value:1.62E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:YGR115C(YGR115C_d)|FD-Score:3.91|P-value:4.67E-5|Clearance:0.29||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YNL114C(YNL114C_d)|FD-Score:7.64|P-value:1.09E-14|Clearance:1.33||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:ASA1(YPR085C)|FD-Score:6.2|P-value:2.76E-10|Clearance:0.51||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CCT4(YDL143W)|FD-Score:3.5|P-value:2.35E-4|Clearance:0.22||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC12(YHR107C)|FD-Score:3.26|P-value:5.58E-4|Clearance:0.02||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:DBP2(YNL112W)|FD-Score:3.61|P-value:1.52E-4|Clearance:0.02||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:GPN2(YOR262W)|FD-Score:4.6|P-value:2.10E-6|Clearance:0.69||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:GPN3(YLR243W_p)|FD-Score:8.48|P-value:1.13E-17|Clearance:0.84||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p Gene:PUP1(YOR157C)|FD-Score:5.03|P-value:2.47E-7|Clearance:0.43||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RBA50(YDR527W)|FD-Score:5.69|P-value:6.25E-9|Clearance:0.66||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RET1(YOR207C)|FD-Score:11.7|P-value:3.92E-32|Clearance:3.26||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPL10(YLR075W)|FD-Score:-3.6|P-value:1.58E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPT6(YGL048C)|FD-Score:3.24|P-value:6.08E-4|Clearance:0.49||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RTP1(YMR185W_p)|FD-Score:6.31|P-value:1.40E-10|Clearance:0.11||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SCC2(YDR180W)|FD-Score:-3.38|P-value:3.62E-4|Clearance:0||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:SEC26(YDR238C)|FD-Score:3.6|P-value:1.62E-4|Clearance:0.1||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SLD3(YGL113W)|FD-Score:-3.38|P-value:3.64E-4|Clearance:0||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPC42(YKL042W)|FD-Score:3.28|P-value:5.25E-4|Clearance:0.02||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:TAP42(YMR028W)|FD-Score:-3.95|P-value:3.99E-5|Clearance:0||SGD DESC:Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits Gene:TIF11(YMR260C)|FD-Score:3.92|P-value:4.51E-5|Clearance:0.01||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:TIF35(YDR429C)|FD-Score:-3.47|P-value:2.62E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TOM22(YNL131W)|FD-Score:3.6|P-value:1.62E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:YGR115C(YGR115C_d)|FD-Score:3.91|P-value:4.67E-5|Clearance:0.29||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YNL114C(YNL114C_d)|FD-Score:7.64|P-value:1.09E-14|Clearance:1.33||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6151020
Download HOP data (tab-delimited text)  (excel)
Gene:AIR2(YDL175C)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:ALF1(YNL148C)|FD-Score:8.3|P-value:5.15E-17||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ARP6(YLR085C)|FD-Score:5.8|P-value:3.41E-9||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:ASF1(YJL115W)|FD-Score:-3.09|P-value:9.88E-4||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:BSC5(YNR069C)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:CIN1(YOR349W)|FD-Score:10.3|P-value:2.55E-25||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CIN2(YPL241C)|FD-Score:5.8|P-value:3.27E-9||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLA4(YNL298W)|FD-Score:8.78|P-value:7.89E-19||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLC1(YGR167W)|FD-Score:4.94|P-value:4.01E-7||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CSF1(YLR087C)|FD-Score:3.87|P-value:5.46E-5||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CYS3(YAL012W)|FD-Score:5.85|P-value:2.47E-9||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:EFG1(YGR271C-A)|FD-Score:-4.74|P-value:1.05E-6||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ERG4(YGL012W)|FD-Score:6.42|P-value:6.71E-11||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FMP48(YGR052W_p)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FUI1(YBL042C)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GEP4(YHR100C)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GGC1(YDL198C)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:GRX6(YDL010W)|FD-Score:4.27|P-value:9.95E-6||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:HTZ1(YOL012C)|FD-Score:8.69|P-value:1.81E-18||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:ISR1(YPR106W)|FD-Score:-4.28|P-value:9.50E-6||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:LEM3(YNL323W)|FD-Score:5.67|P-value:7.00E-9||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LTE1(YAL024C)|FD-Score:16.6|P-value:3.11E-62||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MRT4(YKL009W)|FD-Score:-4.28|P-value:9.34E-6||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:PAC10(YGR078C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAC2(YER007W)|FD-Score:9.98|P-value:8.87E-24||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PAP2(YOL115W)|FD-Score:-3.1|P-value:9.70E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PEP12(YOR036W)|FD-Score:4.87|P-value:5.63E-7||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP3(YLR148W)|FD-Score:4.43|P-value:4.75E-6||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:5.19|P-value:1.07E-7||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:RAV2(YDR202C)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RPS17A(YML024W)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RPS7A(YOR096W)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SCJ1(YMR214W)|FD-Score:-3.2|P-value:6.84E-4||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SEC22(YLR268W)|FD-Score:4.87|P-value:5.72E-7||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SFB3(YHR098C)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SLT2(YHR030C)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SPF1(YEL031W)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SSN3(YPL042C)|FD-Score:-4.28|P-value:9.33E-6||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:SST2(YLR452C)|FD-Score:-3.25|P-value:5.77E-4||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:STO1(YMR125W)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SWC5(YBR231C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TIF2(YJL138C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:37.6|P-value:0||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:11.8|P-value:1.59E-32||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:URE2(YNL229C)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VMA9(YCL005W-A)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VMR1(YHL035C)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VPS3(YDR495C)|FD-Score:3.75|P-value:8.81E-5||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS71(YML041C)|FD-Score:5.13|P-value:1.45E-7||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS72(YDR485C)|FD-Score:10.5|P-value:3.89E-26||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YDR008C(YDR008C_d)|FD-Score:-3.09|P-value:9.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR222W(YDR222W_p)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YKE2(YLR200W)|FD-Score:8.31|P-value:4.82E-17||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YLR279W(YLR279W_d)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR366W(YLR366W_d)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YML094C-A(YML094C-A_d)|FD-Score:3.73|P-value:9.51E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YMR031W-A(YMR031W-A_d)|FD-Score:3.09|P-value:9.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YNL190W(YNL190W_p)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YNL198C(YNL198C_d)|FD-Score:-3.11|P-value:9.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL029C(YOL029C_p)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens Gene:YPK1(YKL126W)|FD-Score:5.2|P-value:9.77E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:AIR2(YDL175C)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:ALF1(YNL148C)|FD-Score:8.3|P-value:5.15E-17||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ARP6(YLR085C)|FD-Score:5.8|P-value:3.41E-9||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:ASF1(YJL115W)|FD-Score:-3.09|P-value:9.88E-4||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:BSC5(YNR069C)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:CIN1(YOR349W)|FD-Score:10.3|P-value:2.55E-25||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CIN2(YPL241C)|FD-Score:5.8|P-value:3.27E-9||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLA4(YNL298W)|FD-Score:8.78|P-value:7.89E-19||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLC1(YGR167W)|FD-Score:4.94|P-value:4.01E-7||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CSF1(YLR087C)|FD-Score:3.87|P-value:5.46E-5||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CYS3(YAL012W)|FD-Score:5.85|P-value:2.47E-9||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:EFG1(YGR271C-A)|FD-Score:-4.74|P-value:1.05E-6||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ERG4(YGL012W)|FD-Score:6.42|P-value:6.71E-11||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FMP48(YGR052W_p)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FUI1(YBL042C)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GEP4(YHR100C)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GGC1(YDL198C)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:GRX6(YDL010W)|FD-Score:4.27|P-value:9.95E-6||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:HTZ1(YOL012C)|FD-Score:8.69|P-value:1.81E-18||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:ISR1(YPR106W)|FD-Score:-4.28|P-value:9.50E-6||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:LEM3(YNL323W)|FD-Score:5.67|P-value:7.00E-9||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LTE1(YAL024C)|FD-Score:16.6|P-value:3.11E-62||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MRT4(YKL009W)|FD-Score:-4.28|P-value:9.34E-6||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:PAC10(YGR078C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAC2(YER007W)|FD-Score:9.98|P-value:8.87E-24||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PAP2(YOL115W)|FD-Score:-3.1|P-value:9.70E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PEP12(YOR036W)|FD-Score:4.87|P-value:5.63E-7||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP3(YLR148W)|FD-Score:4.43|P-value:4.75E-6||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:5.19|P-value:1.07E-7||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:RAV2(YDR202C)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RPS17A(YML024W)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RPS7A(YOR096W)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:SAC6(YDR129C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SCJ1(YMR214W)|FD-Score:-3.2|P-value:6.84E-4||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SEC22(YLR268W)|FD-Score:4.87|P-value:5.72E-7||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SFB3(YHR098C)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SLT2(YHR030C)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SPF1(YEL031W)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SSN3(YPL042C)|FD-Score:-4.28|P-value:9.33E-6||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:SST2(YLR452C)|FD-Score:-3.25|P-value:5.77E-4||SGD DESC:GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family Gene:STO1(YMR125W)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SWC5(YBR231C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TIF2(YJL138C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:37.6|P-value:0||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:11.8|P-value:1.59E-32||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:URE2(YNL229C)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VMA9(YCL005W-A)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VMR1(YHL035C)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VPS3(YDR495C)|FD-Score:3.75|P-value:8.81E-5||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS71(YML041C)|FD-Score:5.13|P-value:1.45E-7||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS72(YDR485C)|FD-Score:10.5|P-value:3.89E-26||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YDR008C(YDR008C_d)|FD-Score:-3.09|P-value:9.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR222W(YDR222W_p)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YKE2(YLR200W)|FD-Score:8.31|P-value:4.82E-17||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YLR279W(YLR279W_d)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR366W(YLR366W_d)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YML094C-A(YML094C-A_d)|FD-Score:3.73|P-value:9.51E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YMR031W-A(YMR031W-A_d)|FD-Score:3.09|P-value:9.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YNL190W(YNL190W_p)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YNL198C(YNL198C_d)|FD-Score:-3.11|P-value:9.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL029C(YOL029C_p)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens Gene:YPK1(YKL126W)|FD-Score:5.2|P-value:9.77E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YOR207C11.703.92E-323.26RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YLR243W_p8.481.13E-170.84GPN3_pPutative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
YNL114C_d7.641.09E-141.33YNL114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit
YMR185W_p6.311.40E-100.11RTP1_pProtein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene
YPR085C6.202.76E-100.51ASA1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
YDR527W5.696.25E-90.66RBA50Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
YOR157C5.032.47E-70.43PUP1Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
YOR262W4.602.10E-60.69GPN2Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion
YMR260C3.924.51E-50.01TIF11Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3
YGR115C_d3.914.67E-50.29YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YNL112W3.611.52E-40.02DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YNL131W3.601.62E-42.77E-4TOM22Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes
YDR238C3.601.62E-40.10SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YDL143W3.502.35E-40.22CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YKL042W3.285.25E-40.02SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane

Top 15 HOM fitness defect scores (HOP)

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Deletion Strain FD score P-value Gene Gene Description
YBR058C-A37.600TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YAL024C16.603.11E-62LTE1Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
YML124C11.801.59E-32TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YDR485C10.503.89E-26VPS72Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
YOR349W10.302.55E-25CIN1Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
YER007W9.988.87E-24PAC2Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
YNL298W8.787.89E-19CLA4Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p
YOL012C8.691.81E-18HTZ1Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin
YLR200W8.314.82E-17YKE2Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
YNL148C8.305.15E-17ALF1Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
YGL012W6.426.71E-11ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YAL012W5.852.47E-9CYS3Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress
YPL241C5.803.27E-9CIN2GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2
YLR085C5.803.41E-9ARP6Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
YNL323W5.677.00E-9LEM3Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane

GO enrichment analysis for SGTC_897
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.6520SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.140845
0.6160SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.112676
0.6100SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.130435
0.5660SGTC_32829108498 15.5 μMChembridge (Drug-like library)164596450.144928
0.5650SGTC_5891227-0070 34.1 μMChemDiv (Drug-like library)28413840.116667
0.5470SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.05TSC3-RPN4
0.5300SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.19403TSC3-RPN4
0.5210SGTC_21415621638 7.7 μMChembridge (Fragment library)28562380.107692tubulin folding & SWR complex
0.5150SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0847458TSC3-RPN4
0.5090SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.12963TSC3-RPN4
0.5050SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.152542
0.4990SGTC_22006600604 21.9 μMChembridge (Fragment library)29047770.109091tubulin folding & SWR complex
0.4920SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.133333TSC3-RPN4
0.4790SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.181818TSC3-RPN4
0.4613.38E-307SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0735294TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_20535265679138 μM0.477273764595Chembridge (Fragment library)233.286242.55113
SGTC_8261683-5028118 μM0.456522722377ChemDiv (Drug-like library)261.703583.26212
SGTC_1090132-003633.7 μM0.44680967274ChemDiv (Drug-like library)293.31663.26423
SGTC_20645220758200 μM0.408163788518Chembridge (Fragment library)263.312221.92615
SGTC_9363005-4620401 μM0.396226777207ChemDiv (Drug-like library)295.296021.48515
SGTC_3067911743149.47 μM0.38775517150885Chembridge (Drug-like library)285.337663.30813
SGTC_21625663267200 μM0.3725496456493Chembridge (Fragment library)298.78843.05625
SGTC_3251913504749.47 μM0.36206917682963Chembridge (Drug-like library)325.38492.4021560S ribosome export
SGTC_3250913558349.47 μM0.367699353Chembridge (Drug-like library)299.321182.80214
SGTC_22077258803200 μM0.3555563666799Chembridge (Fragment library)244.08521.68212