1112-0045

1-(azepan-1-yl)-3-carbazol-9-ylpropan-2-ol

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_900
Screen concentration 141.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 56211
SMILES C1CCCN(CC1)CC(CN2C3=CC=CC=C3C4=CC=CC=C42)O
Standardized SMILES OC(CN1CCCCCC1)Cn2c3ccccc3c4ccccc24
Molecular weight 322.4439
ALogP 4.53
H-bond donor count 1
H-bond acceptor count 2
Response signature NEO1

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.32
% growth inhibition (Hom. pool) 7.92


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 56211
Download HIP data (tab-delimited text)  (excel)
Gene:CCT3(YJL014W)|FD-Score:4.74|P-value:1.09E-6|Clearance:0.07||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CIA1(YDR267C)|FD-Score:-3.21|P-value:6.70E-4|Clearance:0||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein Gene:FRS2(YFL022C)|FD-Score:-4.3|P-value:8.57E-6|Clearance:0||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:GLN1(YPR035W)|FD-Score:-3.21|P-value:6.66E-4|Clearance:0||SGD DESC:Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ILV3(YJR016C)|FD-Score:4.67|P-value:1.52E-6|Clearance:0.45||SGD DESC:Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids Gene:IQG1(YPL242C)|FD-Score:3.41|P-value:3.20E-4|Clearance:0.37||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:NEO1(YIL048W)|FD-Score:40.5|P-value:0|Clearance:31.04||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:RFA3(YJL173C)|FD-Score:-3.42|P-value:3.19E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RIO1(YOR119C)|FD-Score:-6.39|P-value:8.36E-11|Clearance:0||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:RPN6(YDL097C)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.18||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:SMC1(YFL008W)|FD-Score:-5.38|P-value:3.69E-8|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:SUA5(YGL169W)|FD-Score:3.51|P-value:2.26E-4|Clearance:0.09||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:TRL1(YJL087C)|FD-Score:4.22|P-value:1.21E-5|Clearance:0.49||SGD DESC:tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes Gene:UTP9(YHR196W)|FD-Score:9.42|P-value:2.31E-21|Clearance:4.68||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YNL114C(YNL114C_d)|FD-Score:3.73|P-value:9.44E-5|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YPP1(YGR198W)|FD-Score:-3.18|P-value:7.31E-4|Clearance:0||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene Gene:CCT3(YJL014W)|FD-Score:4.74|P-value:1.09E-6|Clearance:0.07||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CIA1(YDR267C)|FD-Score:-3.21|P-value:6.70E-4|Clearance:0||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein Gene:FRS2(YFL022C)|FD-Score:-4.3|P-value:8.57E-6|Clearance:0||SGD DESC:Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar Gene:GLN1(YPR035W)|FD-Score:-3.21|P-value:6.66E-4|Clearance:0||SGD DESC:Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ILV3(YJR016C)|FD-Score:4.67|P-value:1.52E-6|Clearance:0.45||SGD DESC:Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids Gene:IQG1(YPL242C)|FD-Score:3.41|P-value:3.20E-4|Clearance:0.37||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:NEO1(YIL048W)|FD-Score:40.5|P-value:0|Clearance:31.04||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:RFA3(YJL173C)|FD-Score:-3.42|P-value:3.19E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RIO1(YOR119C)|FD-Score:-6.39|P-value:8.36E-11|Clearance:0||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:RPN6(YDL097C)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.18||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:SMC1(YFL008W)|FD-Score:-5.38|P-value:3.69E-8|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:SUA5(YGL169W)|FD-Score:3.51|P-value:2.26E-4|Clearance:0.09||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:TRL1(YJL087C)|FD-Score:4.22|P-value:1.21E-5|Clearance:0.49||SGD DESC:tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes Gene:UTP9(YHR196W)|FD-Score:9.42|P-value:2.31E-21|Clearance:4.68||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YNL114C(YNL114C_d)|FD-Score:3.73|P-value:9.44E-5|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YPP1(YGR198W)|FD-Score:-3.18|P-value:7.31E-4|Clearance:0||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 56211
Download HOP data (tab-delimited text)  (excel)
Gene:ACH1(YBL015W)|FD-Score:-3.86|P-value:5.62E-5||SGD DESC:Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Gene:AFT1(YGL071W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARC18(YLR370C)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARG80(YMR042W)|FD-Score:-4.67|P-value:1.48E-6||SGD DESC:Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p Gene:CCR4(YAL021C)|FD-Score:4.58|P-value:2.29E-6||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CDC50(YCR094W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CKB1(YGL019W)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CKB2(YOR039W)|FD-Score:7.39|P-value:7.12E-14||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CLB5(YPR120C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:COA6(YMR244C-A_p)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress Gene:COG6(YNL041C)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ERG2(YMR202W)|FD-Score:6.37|P-value:9.64E-11||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERV14(YGL054C)|FD-Score:7.31|P-value:1.31E-13||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FRK1(YPL141C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:GCY1(YOR120W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GGA2(YHR108W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin Gene:GRX1(YCL035C)|FD-Score:-4.93|P-value:4.11E-7||SGD DESC:Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:GSF2(YML048W)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:HFM1(YGL251C)|FD-Score:3.78|P-value:7.87E-5||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HXT10(YFL011W)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:IRC20(YLR247C)|FD-Score:-3.11|P-value:9.23E-4||SGD DESC:Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC2(YDR112W_d)|FD-Score:4.62|P-value:1.91E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:KRE1(YNL322C)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:LEM3(YNL323W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LSM1(YJL124C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MAL12(YGR292W)|FD-Score:-5.13|P-value:1.47E-7||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MDM20(YOL076W)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MEU1(YLR017W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression Gene:MNL2(YLR057W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MRPL13(YKR006C)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MSS1(YMR023C)|FD-Score:-4.21|P-value:1.30E-5||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Gene:NEM1(YHR004C)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NHX1(YDR456W)|FD-Score:5.5|P-value:1.95E-8||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NIS1(YNL078W)|FD-Score:-3.38|P-value:3.62E-4||SGD DESC:Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network Gene:PAT1(YCR077C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PEP12(YOR036W)|FD-Score:4.43|P-value:4.61E-6||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP8(YJL053W)|FD-Score:6.45|P-value:5.43E-11||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:RAM1(YDL090C)|FD-Score:5.7|P-value:6.09E-9||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RCY1(YJL204C)|FD-Score:14.1|P-value:1.57E-45||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RNH203(YLR154C)|FD-Score:-3.81|P-value:7.01E-5||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome Gene:RPS10A(YOR293W)|FD-Score:6.38|P-value:9.04E-11||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication Gene:RPS23A(YGR118W)|FD-Score:3.79|P-value:7.40E-5||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RPS4A(YJR145C)|FD-Score:5.45|P-value:2.45E-8||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:SAC3(YDR159W)|FD-Score:3.13|P-value:8.59E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SAC6(YDR129C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SHE4(YOR035C)|FD-Score:3.77|P-value:8.21E-5||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIS2(YKR072C)|FD-Score:10.7|P-value:3.11E-27||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SKN7(YHR206W)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLA1(YBL007C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SNF7(YLR025W)|FD-Score:5.09|P-value:1.82E-7||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNX3(YOR357C)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:STP2(YHR006W)|FD-Score:-3.84|P-value:6.08E-5||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:ULS1(YOR191W)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:VAM10(YOR068C)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VPS20(YMR077C)|FD-Score:6.29|P-value:1.63E-10||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS24(YKL041W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS25(YJR102C)|FD-Score:4.84|P-value:6.39E-7||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS29(YHR012W)|FD-Score:6.56|P-value:2.65E-11||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS3(YDR495C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS35(YJL154C)|FD-Score:7.74|P-value:5.05E-15||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS45(YGL095C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YAR1(YPL239W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YBL086C(YBL086C_p)|FD-Score:-3.95|P-value:3.97E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YBR184W(YBR184W_p)|FD-Score:-3.83|P-value:6.28E-5||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YDL180W(YDL180W_p)|FD-Score:-3.93|P-value:4.27E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR133C(YDR133C_d)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C Gene:YDR455C(YDR455C_d)|FD-Score:9.53|P-value:8.05E-22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YER038W-A(YER038W-A_d)|FD-Score:-3.17|P-value:7.70E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YER119C-A(YER119C-A_d)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGL072C(YGL072C_d)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL081W(YGL081W_p)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis Gene:YJL022W(YJL022W_d)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YJR079W(YJR079W_p)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YJR129C(YJR129C_p)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLL058W(YLL058W_p)|FD-Score:-3.18|P-value:7.29E-4||SGD DESC:Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene Gene:YLR031W(YLR031W_p)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Putative protein of unknown function Gene:YLR108C(YLR108C_p)|FD-Score:-5.74|P-value:4.67E-9||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication Gene:YML007C-A(YML007C-A_p)|FD-Score:3.89|P-value:5.11E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML079W(YML079W_p)|FD-Score:-5.43|P-value:2.82E-8||SGD DESC:Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm Gene:YPK1(YKL126W)|FD-Score:5.84|P-value:2.68E-9||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR108W-A(YPR108W-A_p)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:ACH1(YBL015W)|FD-Score:-3.86|P-value:5.62E-5||SGD DESC:Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Gene:AFT1(YGL071W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARC18(YLR370C)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARG80(YMR042W)|FD-Score:-4.67|P-value:1.48E-6||SGD DESC:Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p Gene:CCR4(YAL021C)|FD-Score:4.58|P-value:2.29E-6||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CDC50(YCR094W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CKB1(YGL019W)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CKB2(YOR039W)|FD-Score:7.39|P-value:7.12E-14||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CLB5(YPR120C)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:COA6(YMR244C-A_p)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress Gene:COG6(YNL041C)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ERG2(YMR202W)|FD-Score:6.37|P-value:9.64E-11||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERV14(YGL054C)|FD-Score:7.31|P-value:1.31E-13||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FRK1(YPL141C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:GCY1(YOR120W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GGA2(YHR108W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin Gene:GRX1(YCL035C)|FD-Score:-4.93|P-value:4.11E-7||SGD DESC:Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:GSF2(YML048W)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:HFM1(YGL251C)|FD-Score:3.78|P-value:7.87E-5||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HXT10(YFL011W)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:Putative hexose transporter, expressed at low levels and expression is repressed by glucose Gene:IRC20(YLR247C)|FD-Score:-3.11|P-value:9.23E-4||SGD DESC:Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC2(YDR112W_d)|FD-Score:4.62|P-value:1.91E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR111C; null mutant displays increased levels of spontaneous Rad52p foci Gene:KRE1(YNL322C)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:LEM3(YNL323W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LSM1(YJL124C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MAL12(YGR292W)|FD-Score:-5.13|P-value:1.47E-7||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MDM20(YOL076W)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MEU1(YLR017W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression Gene:MNL2(YLR057W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MRPL13(YKR006C)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MSS1(YMR023C)|FD-Score:-4.21|P-value:1.30E-5||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3 Gene:NEM1(YHR004C)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NHX1(YDR456W)|FD-Score:5.5|P-value:1.95E-8||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NIS1(YNL078W)|FD-Score:-3.38|P-value:3.62E-4||SGD DESC:Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network Gene:PAT1(YCR077C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PEP12(YOR036W)|FD-Score:4.43|P-value:4.61E-6||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP8(YJL053W)|FD-Score:6.45|P-value:5.43E-11||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:RAM1(YDL090C)|FD-Score:5.7|P-value:6.09E-9||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RCY1(YJL204C)|FD-Score:14.1|P-value:1.57E-45||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RNH203(YLR154C)|FD-Score:-3.81|P-value:7.01E-5||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome Gene:RPS10A(YOR293W)|FD-Score:6.38|P-value:9.04E-11||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication Gene:RPS23A(YGR118W)|FD-Score:3.79|P-value:7.40E-5||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RPS4A(YJR145C)|FD-Score:5.45|P-value:2.45E-8||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:SAC3(YDR159W)|FD-Score:3.13|P-value:8.59E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SAC6(YDR129C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SHE4(YOR035C)|FD-Score:3.77|P-value:8.21E-5||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIS2(YKR072C)|FD-Score:10.7|P-value:3.11E-27||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SKN7(YHR206W)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLA1(YBL007C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SNF7(YLR025W)|FD-Score:5.09|P-value:1.82E-7||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNX3(YOR357C)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:STP2(YHR006W)|FD-Score:-3.84|P-value:6.08E-5||SGD DESC:Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication Gene:ULS1(YOR191W)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:VAM10(YOR068C)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VPS20(YMR077C)|FD-Score:6.29|P-value:1.63E-10||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS24(YKL041W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS25(YJR102C)|FD-Score:4.84|P-value:6.39E-7||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS29(YHR012W)|FD-Score:6.56|P-value:2.65E-11||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS3(YDR495C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS35(YJL154C)|FD-Score:7.74|P-value:5.05E-15||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS45(YGL095C)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YAR1(YPL239W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock Gene:YBL086C(YBL086C_p)|FD-Score:-3.95|P-value:3.97E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YBR184W(YBR184W_p)|FD-Score:-3.83|P-value:6.28E-5||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YDL180W(YDL180W_p)|FD-Score:-3.93|P-value:4.27E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YDR133C(YDR133C_d)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C Gene:YDR455C(YDR455C_d)|FD-Score:9.53|P-value:8.05E-22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YER038W-A(YER038W-A_d)|FD-Score:-3.17|P-value:7.70E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YER119C-A(YER119C-A_d)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGL072C(YGL072C_d)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL081W(YGL081W_p)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis Gene:YJL022W(YJL022W_d)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YJR079W(YJR079W_p)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Putative protein of unknown function; mutation results in impaired mitochondrial respiration Gene:YJR129C(YJR129C_p)|FD-Score:-3.57|P-value:1.77E-4||SGD DESC:Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLL058W(YLL058W_p)|FD-Score:-3.18|P-value:7.29E-4||SGD DESC:Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene Gene:YLR031W(YLR031W_p)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Putative protein of unknown function Gene:YLR108C(YLR108C_p)|FD-Score:-5.74|P-value:4.67E-9||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication Gene:YML007C-A(YML007C-A_p)|FD-Score:3.89|P-value:5.11E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML079W(YML079W_p)|FD-Score:-5.43|P-value:2.82E-8||SGD DESC:Non-essential protein of unknown function with structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm Gene:YPK1(YKL126W)|FD-Score:5.84|P-value:2.68E-9||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR108W-A(YPR108W-A_p)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL048W40.50031.00NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YHR196W9.422.31E-214.68UTP9Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YJL014W4.741.09E-60.07CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YJR016C4.671.52E-60.45ILV3Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids
YJL087C4.221.21E-50.49TRL1tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes
YNL114C_d3.739.44E-50.04YNL114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit
YDL097C3.691.12E-40.18RPN6Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
YGL169W3.512.26E-40.09SUA5Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family
YPL242C3.413.20E-40.37IQG1Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
YBR060C3.050.001160.02ORC2Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
YIL031W3.030.001230.16ULP2Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate
YDR180W2.870.002080.04SCC2Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin
YGR265W_d2.820.002380.02YGR265W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase
YER157W2.800.002520.03COG3Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YNL267W2.770.002770.02PIK1Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL204C14.101.57E-45RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YKR072C10.703.11E-27SIS2Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis
YDR455C_d9.538.05E-22YDR455C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
YJL154C7.745.05E-15VPS35Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
YOR039W7.397.12E-14CKB2Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
YGL054C7.311.31E-13ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
YHR012W6.562.65E-11VPS29Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
YJL053W6.455.43E-11PEP8Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
YOR293W6.389.04E-11RPS10AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication
YMR202W6.379.64E-11ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YMR077C6.291.63E-10VPS20Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes
YKL126W5.842.68E-9YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YDL090C5.706.09E-9RAM1Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit
YDR456W5.501.95E-8NHX1Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion
YJR145C5.452.45E-8RPS4AProtein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication

GO enrichment analysis for SGTC_900
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.7440SGTC_8880916-0002 7.0 μMChemDiv (Drug-like library)28021380.666667NEO1
0.6680SGTC_1721112-0046 2.6 μMChemDiv (Drug-like library)28021350.666667NEO1
0.6670SGTC_232clozapine 119.0 μMMiscellaneous28180.121212NEO1
0.6640SGTC_5352293-4129 48.2 μMChemDiv (Drug-like library)3961180.0972222NEO1
0.6490SGTC_3014119-0100 24.5 μMChemDiv (Drug-like library)28010.126984NEO1
0.6430SGTC_1991st073000 8.9 μMTimTec (Natural product derivative library)43019690.151899NEO1
0.6200SGTC_1781st038725 66.3 μMTimTec (Natural product derivative library)42702750.0615385NEO1
0.6170SGTC_270tamoxifen 5.5 μMMiscellaneous53760.0967742NEO1
0.6140SGTC_2697mebhydrolin 72.4 μMTimTec (Natural product derivative library)225300.296296NEO1
0.6050SGTC_1665st014639 11.4 μMTimTec (Natural product derivative library)9811330.0823529NEO1
0.5840SGTC_2064546-0025 72.0 μMChemDiv (Drug-like library)66207450.0833333NEO1
0.5530SGTC_2658polymyxin b sulfate 77.0 μMMicrosource (Natural product library)57021050.0884956NEO1
0.5470SGTC_9403232-1287 54.8 μMChemDiv (Drug-like library)15854930.0641026NEO1
0.5470SGTC_11303454-2858 15.9 μMChemDiv (Drug-like library)7156280.163934NEO1
0.5470SGTC_3133474-0040 5.0 μMChemDiv (Drug-like library)10433750.101695NEO1-PIK1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1721112-00462.64 μM0.6666672802135ChemDiv (Drug-like library)391.334065.85912NEO1
SGTC_8880916-00026.97 μM0.6666672802138ChemDiv (Drug-like library)377.307485.40312NEO1
SGTC_14414106-004013 μM0.6444442904180ChemDiv (Drug-like library)433.973025.36213
SGTC_8870885-00079.62 μM0.6304355719458ChemDiv (Drug-like library)425.565245.16513endomembrane recycling
SGTC_10084106-00376 μM0.582905394ChemDiv (Drug-like library)443.580525.23214PDR1
SGTC_10094106-00579.87 μM0.582928005ChemDiv (Drug-like library)443.580525.0314
SGTC_9352996-0153430 μM0.48888923738523ChemDiv (Drug-like library)309.402124.91612PDR1
SGTC_11991638-00104.46 μM0.4468093111136ChemDiv (Drug-like library)344.449464.52622endomembrane recycling
SGTC_12021988-006829.6 μM0.4310342850480ChemDiv (Drug-like library)426.555.45913RNA processing & uracil transport
SGTC_14801070-004441.2 μM0.3829793124987ChemDiv (Drug-like library)294.390783.41702