1112-0062

3-(1,2,3,4-tetrahydrocarbazol-9-yl)propanenitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_901
Screen concentration 60.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 1908457
SMILES C1CCC2=C(C1)C3=CC=CC=C3N2CCC#N
Standardized SMILES N#CCCn1c2CCCCc2c3ccccc13
Molecular weight 224.3009
ALogP 3.69
H-bond donor count 0
H-bond acceptor count 1
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 20.67
% growth inhibition (Hom. pool) 6.91


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1908457
Download HIP data (tab-delimited text)  (excel)
Gene:BRL1(YHR036W)|FD-Score:3.24|P-value:5.97E-4|Clearance:0.02||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CDC31(YOR257W)|FD-Score:3.59|P-value:1.62E-4|Clearance:0.23||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CDS1(YBR029C)|FD-Score:5.89|P-value:1.89E-9|Clearance:1.11||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CSE1(YGL238W)|FD-Score:3.17|P-value:7.52E-4|Clearance:0.04||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:HRR25(YPL204W)|FD-Score:3.85|P-value:5.91E-5|Clearance:0.11||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:KRE29(YER038C)|FD-Score:3.22|P-value:6.37E-4|Clearance:0.05||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:PGA1(YNL158W)|FD-Score:3.13|P-value:8.64E-4|Clearance:0.11||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:RPS2(YGL123W)|FD-Score:4.78|P-value:8.78E-7|Clearance:0.33||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS20(YHL015W)|FD-Score:3.74|P-value:9.09E-5|Clearance:0.15||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RRP9(YPR137W)|FD-Score:3.37|P-value:3.80E-4|Clearance:0.13||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SRB4(YER022W)|FD-Score:-4.14|P-value:1.76E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:TAF12(YDR145W)|FD-Score:4.45|P-value:4.31E-6|Clearance:0.02||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:USO1(YDL058W)|FD-Score:4.43|P-value:4.63E-6|Clearance:0.58||SGD DESC:Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex Gene:BRL1(YHR036W)|FD-Score:3.24|P-value:5.97E-4|Clearance:0.02||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CDC31(YOR257W)|FD-Score:3.59|P-value:1.62E-4|Clearance:0.23||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CDS1(YBR029C)|FD-Score:5.89|P-value:1.89E-9|Clearance:1.11||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CSE1(YGL238W)|FD-Score:3.17|P-value:7.52E-4|Clearance:0.04||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:HRR25(YPL204W)|FD-Score:3.85|P-value:5.91E-5|Clearance:0.11||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:KRE29(YER038C)|FD-Score:3.22|P-value:6.37E-4|Clearance:0.05||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:PGA1(YNL158W)|FD-Score:3.13|P-value:8.64E-4|Clearance:0.11||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:RPS2(YGL123W)|FD-Score:4.78|P-value:8.78E-7|Clearance:0.33||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS20(YHL015W)|FD-Score:3.74|P-value:9.09E-5|Clearance:0.15||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RRP9(YPR137W)|FD-Score:3.37|P-value:3.80E-4|Clearance:0.13||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SRB4(YER022W)|FD-Score:-4.14|P-value:1.76E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:TAF12(YDR145W)|FD-Score:4.45|P-value:4.31E-6|Clearance:0.02||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:USO1(YDL058W)|FD-Score:4.43|P-value:4.63E-6|Clearance:0.58||SGD DESC:Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1908457
Download HOP data (tab-delimited text)  (excel)
Gene:AIM13(YFR011C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:AKR1(YDR264C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:BCK2(YER167W)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:CCW12(YLR110C)|FD-Score:4.8|P-value:8.00E-7||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CSF1(YLR087C)|FD-Score:4.5|P-value:3.47E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DAL81(YIR023W)|FD-Score:-3.1|P-value:9.65E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBF2(YGR092W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DSE4(YNR067C)|FD-Score:-3.54|P-value:2.04E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother Gene:ENV10(YLR065C)|FD-Score:-4.66|P-value:1.58E-6||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ETR1(YBR026C)|FD-Score:3.73|P-value:9.75E-5||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:FEN2(YCR028C)|FD-Score:-3.11|P-value:9.23E-4||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FUS2(YMR232W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GCN20(YFR009W)|FD-Score:6.99|P-value:1.37E-12||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCR2(YNL199C)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GGC1(YDL198C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:JJJ3(YJR097W)|FD-Score:4.63|P-value:1.84E-6||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:LYS2(YBR115C)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p Gene:MAL13(YGR288W)|FD-Score:4.05|P-value:2.53E-5||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MLC2(YPR188C)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring Gene:OPY2(YPR075C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:PMT3(YOR321W)|FD-Score:-4.15|P-value:1.66E-5||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:PTC7(YHR076W)|FD-Score:5.3|P-value:5.69E-8||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:QDR2(YIL121W)|FD-Score:-3.81|P-value:6.89E-5||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper Gene:RCE1(YMR274C)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RCN2(YOR220W_p)|FD-Score:6.32|P-value:1.32E-10||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress Gene:RPN4(YDL020C)|FD-Score:-4.73|P-value:1.14E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS26B(YER131W)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication Gene:SED4(YCR067C)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p Gene:SHH4(YLR164W)|FD-Score:-6.11|P-value:4.91E-10||SGD DESC:Mitochondrial inner membrane protein of unknown function; similar to Tim18p and Sdh4p; a fraction copurifies with Sdh3p, but Shh4p is neither a stoichiometric subunit of succinate dehydrogenase nor of the TIM22 translocase; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner Gene:SWC5(YBR231C)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TCB1(YOR086C)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TRI1(YMR233W)|FD-Score:3.75|P-value:8.76E-5||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:3.73|P-value:9.50E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP4(YDR354W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:URA1(YKL216W)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VMA1(YDL185W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA9(YCL005W-A)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:YCR006C(YCR006C_d)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR290W(YDR290W_d)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YLR366W(YLR366W_d)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YMR173W-A(YMR173W-A_d)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YPL102C(YPL102C_d)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:AIM13(YFR011C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:AKR1(YDR264C)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:BCK2(YER167W)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:CCW12(YLR110C)|FD-Score:4.8|P-value:8.00E-7||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CSF1(YLR087C)|FD-Score:4.5|P-value:3.47E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DAL81(YIR023W)|FD-Score:-3.1|P-value:9.65E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBF2(YGR092W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DSE4(YNR067C)|FD-Score:-3.54|P-value:2.04E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother Gene:ENV10(YLR065C)|FD-Score:-4.66|P-value:1.58E-6||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:ETR1(YBR026C)|FD-Score:3.73|P-value:9.75E-5||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:FEN2(YCR028C)|FD-Score:-3.11|P-value:9.23E-4||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FUS2(YMR232W)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GCN20(YFR009W)|FD-Score:6.99|P-value:1.37E-12||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCR2(YNL199C)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GGC1(YDL198C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:JJJ3(YJR097W)|FD-Score:4.63|P-value:1.84E-6||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:LYS2(YBR115C)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p Gene:MAL13(YGR288W)|FD-Score:4.05|P-value:2.53E-5||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MLC2(YPR188C)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring Gene:OPY2(YPR075C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:PMT3(YOR321W)|FD-Score:-4.15|P-value:1.66E-5||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:PTC7(YHR076W)|FD-Score:5.3|P-value:5.69E-8||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:QDR2(YIL121W)|FD-Score:-3.81|P-value:6.89E-5||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper Gene:RCE1(YMR274C)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RCN2(YOR220W_p)|FD-Score:6.32|P-value:1.32E-10||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress Gene:RPN4(YDL020C)|FD-Score:-4.73|P-value:1.14E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS26B(YER131W)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication Gene:SED4(YCR067C)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Integral endoplasmic reticulum membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; similar to Sec12p Gene:SHH4(YLR164W)|FD-Score:-6.11|P-value:4.91E-10||SGD DESC:Mitochondrial inner membrane protein of unknown function; similar to Tim18p and Sdh4p; a fraction copurifies with Sdh3p, but Shh4p is neither a stoichiometric subunit of succinate dehydrogenase nor of the TIM22 translocase; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner Gene:SWC5(YBR231C)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TCB1(YOR086C)|FD-Score:-3.18|P-value:7.38E-4||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TRI1(YMR233W)|FD-Score:3.75|P-value:8.76E-5||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:3.73|P-value:9.50E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP4(YDR354W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:URA1(YKL216W)|FD-Score:-3.11|P-value:9.38E-4||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VMA1(YDL185W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA9(YCL005W-A)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:YCR006C(YCR006C_d)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR290W(YDR290W_d)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YLR366W(YLR366W_d)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YMR173W-A(YMR173W-A_d)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YPL102C(YPL102C_d)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR029C5.891.89E-91.11CDS1Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids
YGL123W4.788.78E-70.33RPS2Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5
YDR145W4.454.31E-60.02TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YDL058W4.434.63E-60.58USO1Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
YPL204W3.855.91E-50.11HRR25Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)
YHL015W3.749.09E-50.15RPS20Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
YOR257W3.591.62E-40.23CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YPR137W3.373.80E-40.13RRP9Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
YHR036W3.245.97E-40.02BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YER038C3.226.37E-40.05KRE29Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
YGL238W3.177.52E-40.04CSE1Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation
YNL158W3.138.64E-40.11PGA1Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes
YCL059C3.020.001240.04KRR1Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit
YDL087C2.990.001410.10LUC7Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA
YNR017W2.890.001950.02TIM23Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YFR009W6.991.37E-12GCN20Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
YOR220W_p6.321.32E-10RCN2_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress
YHR076W5.305.69E-8PTC7Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope
YLR110C4.808.00E-7CCW12Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication
YJR097W4.631.84E-6JJJ3Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4
YLR087C4.503.47E-6CSF1Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YER167W4.191.38E-5BCK2Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations
YER131W4.082.24E-5RPS26BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication
YGR288W4.052.53E-5MAL13MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C
YDL198C4.032.76E-5GGC1Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family
YPR188C3.865.66E-5MLC2Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring
YMR233W3.758.76E-5TRI1Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication
YER090W3.739.50E-5TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YBR026C3.739.75E-5ETR12-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis
YNL199C3.729.79E-5GCR2Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p

GO enrichment analysis for SGTC_901
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1404.37E-27SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.0461538
0.1271.51E-22SGTC_267212a-hydroxy-5-deoxydehydromunduserone 100.0 μMMicrosource (Natural product library)46208980.0547945
0.1254.05E-22SGTC_2733amorolfine 100.0 μMMiscellaneous542600.0735294plasma membrane duress
0.1232.21E-21SGTC_1904st060225 78.0 μMTimTec (Natural product derivative library)665950.0634921
0.1128.13E-18SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.0714286
0.1063.31E-16SGTC_8900929-0006 47.2 μMChemDiv (Drug-like library)13781690.057971
0.1041.28E-15SGTC_255413-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one 66.7 μMMicrosource (Natural product library)67085610.0769231
0.1024.89E-15SGTC_2741miltefosine 3.0 μMMiscellaneous35990.0163934
0.1019.18E-15SGTC_5831488-1091 88.9 μMChemDiv (Drug-like library)43032730.0447761
0.1019.50E-15SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0434783
0.0993.25E-14SGTC_10410206-0046 107.0 μMChemDiv (Drug-like library)504940.111111
0.0983.52E-14SGTC_500929-0078 56.4 μMChemDiv (Drug-like library)13777510.0847458
0.0977.68E-14SGTC_13391486-1321 22.7 μMChemDiv (Drug-like library)34693820.0684932ERG2
0.0979.94E-14SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0588235
0.0971.01E-13SGTC_24865283202 22.4 μMMiscellaneous13777510.0847458

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_446go6976132 μM0.406783501ICCB bioactive library378.425924.16812
SGTC_2697mebhydrolin72.4 μM0.36538522530TimTec (Natural product derivative library)276.37554.06601NEO1
SGTC_8860866-0150153 μM0.333333271463ChemDiv (Drug-like library)199.248423.14201
SGTC_14801070-004441.2 μM0.2941183124987ChemDiv (Drug-like library)294.390783.41702
SGTC_9001112-0045141 μM0.28846256211ChemDiv (Drug-like library)322.443944.5312NEO1
SGTC_9352996-0153430 μM0.28301923738523ChemDiv (Drug-like library)309.402124.91612PDR1
SGTC_1096rimcazole32.2 μM0.27777818528119NIH Clinical Collection375.93544.08133
SGTC_2739tacrine100.86 μM0.2653061935Miscellaneous198.263662.7912heme requiring
SGTC_23995884133200 μM0.257576722445Miscellaneous319.403543.75814cell wall signaling
SGTC_6631486-073313.9 μM0.257576722445ChemDiv (Drug-like library)319.403543.75814cell wall signaling