2-aminofluorene

9H-fluoren-2-amine

An established DNA mutagen.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_905
Screen concentration 215.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 1539
SMILES C1C2=CC=CC=C2C3=C1C=C(C=C3)N
Standardized SMILES Nc1ccc2c(Cc3ccccc23)c1
Molecular weight 181.2331
ALogP 2.75
H-bond donor count 1
H-bond acceptor count 1
Response signature cell wall signaling

Pool Growth Kinetics
% growth inhibition (Het. pool) 17.37
% growth inhibition (Hom. pool) 13.65


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1539
Download HIP data (tab-delimited text)  (excel)
Gene:CLP1(YOR250C)|FD-Score:3.92|P-value:4.48E-5|Clearance:0.29||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:DIS3(YOL021C)|FD-Score:-4.85|P-value:6.32E-7|Clearance:0||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:IMP3(YHR148W)|FD-Score:3.12|P-value:8.95E-4|Clearance:0.12||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:MCM2(YBL023C)|FD-Score:4.3|P-value:8.42E-6|Clearance:0.13||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MED11(YMR112C)|FD-Score:-3.9|P-value:4.74E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein Gene:PCF11(YDR228C)|FD-Score:3.3|P-value:4.86E-4|Clearance:0.15||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:RHO1(YPR165W)|FD-Score:17.6|P-value:2.91E-69|Clearance:12.14||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPB8(YOR224C)|FD-Score:3.13|P-value:8.69E-4|Clearance:0.01||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC10(YHR143W-A)|FD-Score:-4.08|P-value:2.25E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPC37(YKR025W)|FD-Score:3.43|P-value:3.00E-4|Clearance:0.03||SGD DESC:RNA polymerase III subunit C37 Gene:RPL15A(YLR029C)|FD-Score:-3.2|P-value:6.94E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPL32(YBL092W)|FD-Score:4.18|P-value:1.48E-5|Clearance:0.23||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RTS2(YOR077W)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.2||SGD DESC:Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication Gene:SPC42(YKL042W)|FD-Score:3.95|P-value:3.91E-5|Clearance:0.03||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:TIF11(YMR260C)|FD-Score:-3.14|P-value:8.48E-4|Clearance:0||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:TIM44(YIL022W)|FD-Score:-3.12|P-value:9.13E-4|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) Gene:YAH1(YPL252C)|FD-Score:5.41|P-value:3.08E-8|Clearance:1.11||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YGR073C(YGR073C_d)|FD-Score:3.15|P-value:8.22E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:YKL036C(YKL036C_d)|FD-Score:3.41|P-value:3.30E-4|Clearance:0.11||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W Gene:YOR203W(YOR203W_d)|FD-Score:-3.7|P-value:1.09E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Gene:CLP1(YOR250C)|FD-Score:3.92|P-value:4.48E-5|Clearance:0.29||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:DIS3(YOL021C)|FD-Score:-4.85|P-value:6.32E-7|Clearance:0||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:IMP3(YHR148W)|FD-Score:3.12|P-value:8.95E-4|Clearance:0.12||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:MCM2(YBL023C)|FD-Score:4.3|P-value:8.42E-6|Clearance:0.13||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MED11(YMR112C)|FD-Score:-3.9|P-value:4.74E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein Gene:PCF11(YDR228C)|FD-Score:3.3|P-value:4.86E-4|Clearance:0.15||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:RHO1(YPR165W)|FD-Score:17.6|P-value:2.91E-69|Clearance:12.14||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPB8(YOR224C)|FD-Score:3.13|P-value:8.69E-4|Clearance:0.01||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC10(YHR143W-A)|FD-Score:-4.08|P-value:2.25E-5|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-alpha, found in RNA pol I, II, and III; relocalizes from nucleolus to cytoplasm upon DNA replication stress Gene:RPC37(YKR025W)|FD-Score:3.43|P-value:3.00E-4|Clearance:0.03||SGD DESC:RNA polymerase III subunit C37 Gene:RPL15A(YLR029C)|FD-Score:-3.2|P-value:6.94E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPL32(YBL092W)|FD-Score:4.18|P-value:1.48E-5|Clearance:0.23||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RTS2(YOR077W)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.2||SGD DESC:Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication Gene:SPC42(YKL042W)|FD-Score:3.95|P-value:3.91E-5|Clearance:0.03||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:TIF11(YMR260C)|FD-Score:-3.14|P-value:8.48E-4|Clearance:0||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:TIM44(YIL022W)|FD-Score:-3.12|P-value:9.13E-4|Clearance:0||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) Gene:YAH1(YPL252C)|FD-Score:5.41|P-value:3.08E-8|Clearance:1.11||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YGR073C(YGR073C_d)|FD-Score:3.15|P-value:8.22E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:YKL036C(YKL036C_d)|FD-Score:3.41|P-value:3.30E-4|Clearance:0.11||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W Gene:YOR203W(YOR203W_d)|FD-Score:-3.7|P-value:1.09E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1539
Download HOP data (tab-delimited text)  (excel)
Gene:ADR1(YDR216W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:ALF1(YNL148C)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ARC18(YLR370C)|FD-Score:-3.89|P-value:5.09E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARO1(YDR127W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BCK1(YJL095W)|FD-Score:28.4|P-value:4.10E-177||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BCK2(YER167W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:CHS7(YHR142W)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CPR1(YDR155C)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; protein abundance increases in response to DNA replication stress Gene:CTR1(YPR124W)|FD-Score:3.76|P-value:8.59E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYB5(YNL111C)|FD-Score:3.73|P-value:9.73E-5||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:EFM1(YHL039W)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EMP65(YER140W_p)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ENV9(YOR246C)|FD-Score:6.43|P-value:6.27E-11||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:ERG2(YMR202W)|FD-Score:3.95|P-value:3.97E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:GAL3(YDR009W)|FD-Score:-3.84|P-value:6.12E-5||SGD DESC:Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication Gene:GAL83(YER027C)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:GCN20(YFR009W)|FD-Score:5.03|P-value:2.45E-7||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:6.85|P-value:3.57E-12||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GRX1(YCL035C)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:HTA1(YDR225W)|FD-Score:4.95|P-value:3.69E-7||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IMP2'(YIL154C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC21(YMR073C)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRC5(YFR038W)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:JNM1(YMR294W)|FD-Score:4.79|P-value:8.24E-7||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LSB6(YJL100W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MRPL16(YBL038W)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL51(YPR100W)|FD-Score:3.73|P-value:9.57E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSR1(YHR091C)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 Gene:NTO1(YPR031W)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:NUM1(YDR150W)|FD-Score:3.78|P-value:7.85E-5||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:PHO88(YBR106W)|FD-Score:4.97|P-value:3.27E-7||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PSY3(YLR376C)|FD-Score:4.29|P-value:9.07E-6||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:RAD18(YCR066W)|FD-Score:3.79|P-value:7.44E-5||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD26(YJR035W)|FD-Score:-3.8|P-value:7.26E-5||SGD DESC:Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein Gene:RAD5(YLR032W)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RCY1(YJL204C)|FD-Score:8.8|P-value:6.58E-19||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RMD1(YDL001W)|FD-Score:4.83|P-value:6.95E-7||SGD DESC:Cytoplasmic protein required for sporulation Gene:ROX1(YPR065W)|FD-Score:3.75|P-value:8.74E-5||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RPN4(YDL020C)|FD-Score:6.38|P-value:8.59E-11||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RSB1(YOR049C)|FD-Score:4.64|P-value:1.73E-6||SGD DESC:Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane Gene:RTF1(YGL244W)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SAC6(YDR129C)|FD-Score:-4.02|P-value:2.86E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAM37(YMR060C)|FD-Score:5.11|P-value:1.59E-7||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SFP1(YLR403W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SLG1(YOR008C)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLT2(YHR030C)|FD-Score:18.7|P-value:1.17E-78||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SLX9(YGR081C)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SNA2(YDR525W-A)|FD-Score:3.82|P-value:6.58E-5||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SOK2(YMR016C)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; SOK2 has a paralog, PHD1, that arose from the whole genome duplication Gene:SPT7(YBR081C)|FD-Score:4.39|P-value:5.74E-6||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SWC5(YBR231C)|FD-Score:3.45|P-value:2.75E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TRP3(YKL211C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TUB3(YML124C)|FD-Score:3.84|P-value:6.06E-5||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VAM10(YOR068C)|FD-Score:4.78|P-value:8.82E-7||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VBA2(YBR293W)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS51(YKR020W)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS75(YNL246W)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:YAL004W(YAL004W_d)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YBR219C(YBR219C_p)|FD-Score:3.28|P-value:5.10E-4||SGD DESC:Putative protein of unknown function; YBR219C is not an essential gene Gene:YCL042W(YCL042W_p)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YCR050C(YCR050C_p)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YDL034W(YDL034W_d)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.72|P-value:1.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFR035C(YFR035C_p)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YGL015C(YGL015C_p)|FD-Score:3.81|P-value:6.83E-5||SGD DESC:Putative protein of unknown function; null mutants accumulate cargo in the Golgi Gene:YGL176C(YGL176C_p)|FD-Score:5.24|P-value:7.94E-8||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YHR049C-A(YHR049C-A_d)|FD-Score:-3.87|P-value:5.49E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIH1(YCR059C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YLR363W-A(YLR363W-A_p)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:YLR446W(YLR446W_p)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YNL109W(YNL109W_d)|FD-Score:-3.1|P-value:9.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C Gene:YOL079W(YOL079W_d)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ADR1(YDR216W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:ALF1(YNL148C)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ARC18(YLR370C)|FD-Score:-3.89|P-value:5.09E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARO1(YDR127W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BCK1(YJL095W)|FD-Score:28.4|P-value:4.10E-177||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BCK2(YER167W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:CHS7(YHR142W)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CPR1(YDR155C)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; protein abundance increases in response to DNA replication stress Gene:CTR1(YPR124W)|FD-Score:3.76|P-value:8.59E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYB5(YNL111C)|FD-Score:3.73|P-value:9.73E-5||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:EFM1(YHL039W)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:EMP65(YER140W_p)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ENV9(YOR246C)|FD-Score:6.43|P-value:6.27E-11||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:ERG2(YMR202W)|FD-Score:3.95|P-value:3.97E-5||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:GAL3(YDR009W)|FD-Score:-3.84|P-value:6.12E-5||SGD DESC:Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication Gene:GAL83(YER027C)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:GCN20(YFR009W)|FD-Score:5.03|P-value:2.45E-7||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:6.85|P-value:3.57E-12||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GRX1(YCL035C)|FD-Score:-3.4|P-value:3.31E-4||SGD DESC:Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:HTA1(YDR225W)|FD-Score:4.95|P-value:3.69E-7||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IMP2'(YIL154C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC21(YMR073C)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRC5(YFR038W)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:JNM1(YMR294W)|FD-Score:4.79|P-value:8.24E-7||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LSB6(YJL100W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MRPL16(YBL038W)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL51(YPR100W)|FD-Score:3.73|P-value:9.57E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSR1(YHR091C)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 Gene:NTO1(YPR031W)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:NUM1(YDR150W)|FD-Score:3.78|P-value:7.85E-5||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:PHO88(YBR106W)|FD-Score:4.97|P-value:3.27E-7||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PSY3(YLR376C)|FD-Score:4.29|P-value:9.07E-6||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C Gene:RAD18(YCR066W)|FD-Score:3.79|P-value:7.44E-5||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD26(YJR035W)|FD-Score:-3.8|P-value:7.26E-5||SGD DESC:Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein Gene:RAD5(YLR032W)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RCY1(YJL204C)|FD-Score:8.8|P-value:6.58E-19||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RMD1(YDL001W)|FD-Score:4.83|P-value:6.95E-7||SGD DESC:Cytoplasmic protein required for sporulation Gene:ROX1(YPR065W)|FD-Score:3.75|P-value:8.74E-5||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RPN4(YDL020C)|FD-Score:6.38|P-value:8.59E-11||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RSB1(YOR049C)|FD-Score:4.64|P-value:1.73E-6||SGD DESC:Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane Gene:RTF1(YGL244W)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SAC6(YDR129C)|FD-Score:-4.02|P-value:2.86E-5||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAM37(YMR060C)|FD-Score:5.11|P-value:1.59E-7||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SFP1(YLR403W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SLG1(YOR008C)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLT2(YHR030C)|FD-Score:18.7|P-value:1.17E-78||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SLX9(YGR081C)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SNA2(YDR525W-A)|FD-Score:3.82|P-value:6.58E-5||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SOK2(YMR016C)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; SOK2 has a paralog, PHD1, that arose from the whole genome duplication Gene:SPT7(YBR081C)|FD-Score:4.39|P-value:5.74E-6||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SWC5(YBR231C)|FD-Score:3.45|P-value:2.75E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TRP3(YKL211C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TUB3(YML124C)|FD-Score:3.84|P-value:6.06E-5||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VAM10(YOR068C)|FD-Score:4.78|P-value:8.82E-7||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VBA2(YBR293W)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS51(YKR020W)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS75(YNL246W)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:YAL004W(YAL004W_d)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YBR219C(YBR219C_p)|FD-Score:3.28|P-value:5.10E-4||SGD DESC:Putative protein of unknown function; YBR219C is not an essential gene Gene:YCL042W(YCL042W_p)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YCR050C(YCR050C_p)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YDL034W(YDL034W_d)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.72|P-value:1.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFR035C(YFR035C_p)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YGL015C(YGL015C_p)|FD-Score:3.81|P-value:6.83E-5||SGD DESC:Putative protein of unknown function; null mutants accumulate cargo in the Golgi Gene:YGL176C(YGL176C_p)|FD-Score:5.24|P-value:7.94E-8||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YHR049C-A(YHR049C-A_d)|FD-Score:-3.87|P-value:5.49E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIH1(YCR059C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YLR363W-A(YLR363W-A_p)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:YLR446W(YLR446W_p)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YNL109W(YNL109W_d)|FD-Score:-3.1|P-value:9.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C Gene:YOL079W(YOL079W_d)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YPR165W17.602.91E-6912.10RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YPL252C5.413.08E-81.11YAH1Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
YBL023C4.308.42E-60.13MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YBL092W4.181.48E-50.23RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YKL042W3.953.91E-50.03SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
YOR250C3.924.48E-50.29CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YOR077W3.631.42E-40.20RTS2Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication
YKR025W3.433.00E-40.03RPC37RNA polymerase III subunit C37
YKL036C_d3.413.30E-40.11YKL036C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W
YDR228C3.304.86E-40.15PCF11mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
YGR073C_d3.158.22E-40.02YGR073C_dDubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein
YOR224C3.138.69E-40.01RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YHR148W3.128.95E-40.12IMP3Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA
YDR510W3.000.001340.02SMT3Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2
YBR202W2.980.001430.03MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL095W28.404.10E-177BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YHR030C18.701.17E-78SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YJL204C8.806.58E-19RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YKR026C6.853.57E-12GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YOR246C6.436.27E-11ENV9Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
YDL020C6.388.59E-11RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YGL176C_p5.247.94E-8YGL176C_pPutative protein of unknown function; deletion mutant is viable and has no detectable phenotype
YMR060C5.111.59E-7SAM37Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability
YHL039W5.111.63E-7EFM1Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YFR009W5.032.45E-7GCN20Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
YBR106W4.973.27E-7PHO88Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
YDR225W4.953.69E-7HTA1Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
YDL001W4.836.95E-7RMD1Cytoplasmic protein required for sporulation
YMR294W4.798.24E-7JNM1Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B
YOR068C4.788.82E-7VAM10Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering

GO enrichment analysis for SGTC_905
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.5470SGTC_22727805186 180.0 μMChembridge (Fragment library)29560620.0535714cell wall signaling
0.4750SGTC_20285153890 36.4 μMChembridge (Fragment library)28309680.111111cell wall signaling
0.4451.42E-284SGTC_22107115885 190.4 μMChembridge (Fragment library)29270390.263158cell wall signaling
0.4399.23E-277SGTC_21495554786 194.8 μMChembridge (Fragment library)7450630.163265cell wall signaling
0.4357.36E-271SGTC_6631486-0733 13.9 μMChemDiv (Drug-like library)7224450.0909091cell wall signaling
0.4284.92E-262SGTC_31229126309 49.5 μMChembridge (Drug-like library)175912060.0806452cell wall signaling
0.4143.53E-243SGTC_1874staurosporine 7.6 μMICCB bioactive library468641930.0759494cell wall signaling
0.4078.38E-235SGTC_6420335-0876 158.0 μMChemDiv (Drug-like library)68123500.0819672ubiquinone biosynthesis & proteasome
0.4067.56E-233SGTC_422cantharidin 155.0 μMICCB bioactive library25450.047619cell wall signaling
0.3983.78E-223SGTC_3600957-0399 1.0 μMChemDiv (Drug-like library)67523010.0694444cell wall
0.3979.71E-222SGTC_500h-89 112.0 μMICCB bioactive library4492410.057971cell wall signaling
0.3948.34E-219SGTC_408cantharidin 100.0 μMICCB bioactive library25450.047619cell wall signaling
0.3831.50E-205SGTC_30929117248 49.5 μMChembridge (Drug-like library)171418570.0952381cell wall signaling
0.3686.99E-189SGTC_20094012297 12.1 μMChembridge (Fragment library)455953070.12cell wall signaling
0.3518.50E-171SGTC_20074011539 71.8 μMChembridge (Fragment library)455953010.122449cell wall signaling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20165121758140 μM0.3752795203Chembridge (Fragment library)168.623461.22821redox potentiating
SGTC_1760st04545310.45 μM0.3269233323419TimTec (Natural product derivative library)385.498085.38213NEO1-PIK1
SGTC_1169aniline yellow25.8 μM0.281256051ChemDiv (Drug-like library)197.235843.45213
SGTC_6690108-002126.2 μM0.26470617201ChemDiv (Drug-like library)292.331843.45724fatty acid desaturase (OLE1)
SGTC_22107115885190.39 μM0.2631582927039Chembridge (Fragment library)227.687362.28523cell wall signaling
SGTC_22297354935190 μM0.255814318112Chembridge (Fragment library)237.256641.74722RSC complex & mRNA processing
SGTC_22987976326193.81 μM0.2558142235818Chembridge (Fragment library)203.237121.95623
SGTC_22997976368114.88 μM0.2558142979718Chembridge (Fragment library)223.65562.13423
SGTC_5801123-002841 μM0.251677248ChemDiv (Drug-like library)359.334924.87805DNA damage response
SGTC_1953st07680012.1 μM0.243902297591TimTec (Natural product derivative library)219.281124.53410