3456-4043

6-ethoxy-4-methylidene-3-phenyl-1H-quinazolin-2-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_911
Screen concentration 236.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 914281
SMILES CCOC1=CC2=C(C=C1)NC(=O)N(C2=C)C3=CC=CC=C3
Standardized SMILES CCOc1ccc2NC(=O)N(C(=C)c2c1)c3ccccc3
Molecular weight 280.3211
ALogP 3.06
H-bond donor count 1
H-bond acceptor count 2
Response signature mitochondrial processes

Pool Growth Kinetics
% growth inhibition (Het. pool) 36.77
% growth inhibition (Hom. pool) 8.14


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 914281
Download HIP data (tab-delimited text)  (excel)
Gene:ERB1(YMR049C)|FD-Score:3.78|P-value:7.78E-5|Clearance:0.08||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:ERG11(YHR007C)|FD-Score:4.28|P-value:9.17E-6|Clearance:0.48||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG26(YGL001C)|FD-Score:3.27|P-value:5.44E-4|Clearance:0.03||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:ESP1(YGR098C)|FD-Score:5.04|P-value:2.38E-7|Clearance:0.47||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:FRS1(YLR060W)|FD-Score:4.5|P-value:3.36E-6|Clearance:0.01||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:GDI1(YER136W)|FD-Score:4.32|P-value:7.68E-6|Clearance:0.04||SGD DESC:GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins Gene:GPM1(YKL152C)|FD-Score:-3.87|P-value:5.37E-5|Clearance:0||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:GPN2(YOR262W)|FD-Score:-5.68|P-value:6.69E-9|Clearance:0||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:HSP10(YOR020C)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.2||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:MAK5(YBR142W)|FD-Score:-3.74|P-value:9.18E-5|Clearance:0||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:PMI40(YER003C)|FD-Score:6.48|P-value:4.64E-11|Clearance:0.81||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PRP42(YDR235W)|FD-Score:-3.12|P-value:9.11E-4|Clearance:0||SGD DESC:U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats Gene:RET1(YOR207C)|FD-Score:3.49|P-value:2.45E-4|Clearance:0.02||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPC53(YDL150W)|FD-Score:3.13|P-value:8.63E-4|Clearance:0.04||SGD DESC:RNA polymerase III subunit C53 Gene:RPN3(YER021W)|FD-Score:3.19|P-value:7.08E-4|Clearance:0.06||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPP1(YHR062C)|FD-Score:4.34|P-value:7.14E-6|Clearance:0.02||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT1(YKL145W)|FD-Score:3.81|P-value:6.98E-5|Clearance:0.03||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RSC58(YLR033W)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:3.24|P-value:5.97E-4|Clearance:0.05||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SDS22(YKL193C)|FD-Score:5.67|P-value:7.31E-9|Clearance:0.63||SGD DESC:Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function Gene:SEN15(YMR059W)|FD-Score:4.35|P-value:6.85E-6|Clearance:0.01||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SLD3(YGL113W)|FD-Score:4.57|P-value:2.49E-6|Clearance:0.06||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPN1(YPR133C)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.11||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SPT6(YGR116W)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.03||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:TEL2(YGR099W)|FD-Score:-3.16|P-value:7.92E-4|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TOM40(YMR203W)|FD-Score:9.09|P-value:5.00E-20|Clearance:2.61||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UTR5(YEL035C_p)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.01||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YGR114C(YGR114C_d)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:4.5|P-value:3.47E-6|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:ERB1(YMR049C)|FD-Score:3.78|P-value:7.78E-5|Clearance:0.08||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:ERG11(YHR007C)|FD-Score:4.28|P-value:9.17E-6|Clearance:0.48||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG26(YGL001C)|FD-Score:3.27|P-value:5.44E-4|Clearance:0.03||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:ESP1(YGR098C)|FD-Score:5.04|P-value:2.38E-7|Clearance:0.47||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:FRS1(YLR060W)|FD-Score:4.5|P-value:3.36E-6|Clearance:0.01||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:GDI1(YER136W)|FD-Score:4.32|P-value:7.68E-6|Clearance:0.04||SGD DESC:GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins Gene:GPM1(YKL152C)|FD-Score:-3.87|P-value:5.37E-5|Clearance:0||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:GPN2(YOR262W)|FD-Score:-5.68|P-value:6.69E-9|Clearance:0||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:HSP10(YOR020C)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.2||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:MAK5(YBR142W)|FD-Score:-3.74|P-value:9.18E-5|Clearance:0||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:PMI40(YER003C)|FD-Score:6.48|P-value:4.64E-11|Clearance:0.81||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PRP42(YDR235W)|FD-Score:-3.12|P-value:9.11E-4|Clearance:0||SGD DESC:U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats Gene:RET1(YOR207C)|FD-Score:3.49|P-value:2.45E-4|Clearance:0.02||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPC53(YDL150W)|FD-Score:3.13|P-value:8.63E-4|Clearance:0.04||SGD DESC:RNA polymerase III subunit C53 Gene:RPN3(YER021W)|FD-Score:3.19|P-value:7.08E-4|Clearance:0.06||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPP1(YHR062C)|FD-Score:4.34|P-value:7.14E-6|Clearance:0.02||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT1(YKL145W)|FD-Score:3.81|P-value:6.98E-5|Clearance:0.03||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RSC58(YLR033W)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:3.24|P-value:5.97E-4|Clearance:0.05||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SDS22(YKL193C)|FD-Score:5.67|P-value:7.31E-9|Clearance:0.63||SGD DESC:Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function Gene:SEN15(YMR059W)|FD-Score:4.35|P-value:6.85E-6|Clearance:0.01||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SLD3(YGL113W)|FD-Score:4.57|P-value:2.49E-6|Clearance:0.06||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPN1(YPR133C)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.11||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SPT6(YGR116W)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.03||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:TEL2(YGR099W)|FD-Score:-3.16|P-value:7.92E-4|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TOM40(YMR203W)|FD-Score:9.09|P-value:5.00E-20|Clearance:2.61||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UTR5(YEL035C_p)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.01||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YGR114C(YGR114C_d)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:4.5|P-value:3.47E-6|Clearance:0.15||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 914281
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ACS1(YAL054C)|FD-Score:-3.35|P-value:4.04E-4||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:AIM3(YBR108W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:AIR2(YDL175C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:ANS1(YHR126C_p)|FD-Score:-3.38|P-value:3.62E-4||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:AQY1(YPR192W)|FD-Score:3.83|P-value:6.45E-5||SGD DESC:Spore-specific water channel that mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance Gene:ARN1(YHL040C)|FD-Score:4.56|P-value:2.59E-6||SGD DESC:ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress Gene:ASI1(YMR119W)|FD-Score:4.28|P-value:9.27E-6||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ATG8(YBL078C)|FD-Score:-3.82|P-value:6.78E-5||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BDS1(YOL164W)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BUD19(YJL188C_d)|FD-Score:4.43|P-value:4.67E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUD21(YOR078W)|FD-Score:4.96|P-value:3.55E-7||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:CAN1(YEL063C)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CAP2(YIL034C)|FD-Score:4.83|P-value:6.93E-7||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CBP6(YBR120C)|FD-Score:-3.86|P-value:5.69E-5||SGD DESC:Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:COA2(YPL189C-A)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:CRP1(YHR146W)|FD-Score:3.45|P-value:2.75E-4||SGD DESC:Protein that binds to cruciform DNA structures Gene:CWC15(YDR163W)|FD-Score:-3.1|P-value:9.67E-4||SGD DESC:Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p Gene:DAL2(YIR029W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DFG5(YMR238W)|FD-Score:4.42|P-value:5.02E-6||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DIA2(YOR080W)|FD-Score:5.03|P-value:2.49E-7||SGD DESC:Origin-binding F-box protein; forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication; plays a role in transcription; required for correct assembly of RSC complex, correct RSC-mediated transcription regulation, and correct nucleosome positioning; involved in invasive and pseudohyphal growth; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:DOG2(YHR043C)|FD-Score:-5.23|P-value:8.57E-8||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:DTR1(YBR180W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:DUS1(YML080W)|FD-Score:4.81|P-value:7.46E-7||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17 Gene:ECM23(YPL021W)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p Gene:ECM34(YHL043W)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:EGT2(YNL327W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner Gene:ERJ5(YFR041C)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:FAA1(YOR317W)|FD-Score:-3.28|P-value:5.14E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FAA3(YIL009W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:FYV4(YHR059W)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCY1(YOR120W)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GLE2(YER107C)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GRE2(YOL151W)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:GRR1(YJR090C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GTR2(YGR163W)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GZF3(YJL110C)|FD-Score:-5.76|P-value:4.12E-9||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HCM1(YCR065W)|FD-Score:5.07|P-value:1.96E-7||SGD DESC:Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role Gene:HPA3(YEL066W)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro Gene:HSL7(YBR133C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:HYR1(YIR037W)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:IKI1(YHR187W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IML3(YBR107C)|FD-Score:5.84|P-value:2.64E-9||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:ISM1(YPL040C)|FD-Score:5.34|P-value:4.57E-8||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:JJJ1(YNL227C)|FD-Score:4.82|P-value:7.02E-7||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:KTR7(YIL085C)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:LIN1(YHR156C)|FD-Score:5.56|P-value:1.35E-8||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:MNN11(YJL183W)|FD-Score:6.95|P-value:1.82E-12||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MON2(YNL297C)|FD-Score:-5.66|P-value:7.45E-9||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MPD2(YOL088C)|FD-Score:-7.9|P-value:1.44E-15||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRPS28(YDR337W)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSC7(YHR039C)|FD-Score:3.99|P-value:3.27E-5||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MTQ1(YNL063W)|FD-Score:-4.04|P-value:2.72E-5||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene Gene:NIF3(YGL221C)|FD-Score:5.41|P-value:3.18E-8||SGD DESC:Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PFA3(YNL326C)|FD-Score:4.82|P-value:7.00E-7||SGD DESC:Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions Gene:PUS7(YOR243C)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:PYC1(YGL062W)|FD-Score:-3.36|P-value:3.93E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:QDR3(YBR043C)|FD-Score:3.73|P-value:9.43E-5||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:RAD30(YDR419W)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV Gene:RIF2(YLR453C)|FD-Score:4.86|P-value:5.77E-7||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:ROT2(YBR229C)|FD-Score:5.68|P-value:6.82E-9||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:SAP4(YGL229C)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SEC66(YBR171W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SEE1(YIL064W)|FD-Score:-4.44|P-value:4.54E-6||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SIS2(YKR072C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLM6(YBR266C_d)|FD-Score:6.13|P-value:4.34E-10||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SNQ2(YDR011W)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPG5(YMR191W)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SWI4(YER111C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:SYH1(YPL105C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TRM1(YDR120C)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:UBC11(YOR339C)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:UBC4(YBR082C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication Gene:UBP11(YKR098C)|FD-Score:3.75|P-value:8.91E-5||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins Gene:URA2(YJL130C)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:USV1(YPL230W)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Putative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in growth on non-fermentable carbon sources, response to salt stress and cell wall biosynthesis; USV1 has a paralog, RGM1, that arose from the whole genome duplication Gene:YAR023C(YAR023C_p)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family Gene:YBL100W-C(YBL100W-C_p)|FD-Score:-3.82|P-value:6.60E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR023C(YCR023C)|FD-Score:-3.18|P-value:7.49E-4||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YDR169C-A(YDR169C-A_p)|FD-Score:-5.85|P-value:2.50E-9||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YFH7(YFR007W)|FD-Score:3.98|P-value:3.47E-5||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YFR032C-B(YFR032C-B_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL072C(YGL072C_d)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR069W(YGR069W_d)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR086W-A(YHR086W-A_p)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIL092W(YIL092W_p)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YKL075C(YKL075C_p)|FD-Score:-3.17|P-value:7.72E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YLL054C(YLL054C_p)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YLR224W(YLR224W_p)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene Gene:YML007C-A(YML007C-A_p)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML122C(YML122C_d)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR085W(YMR085W_p)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YNL155W(YNL155W_p)|FD-Score:5.78|P-value:3.78E-9||SGD DESC:Protein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOL024W(YOL024W_p)|FD-Score:-4.15|P-value:1.67E-5||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication Gene:YOR296W(YOR296W_p)|FD-Score:-3.74|P-value:9.09E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene Gene:YPL039W(YPL039W_p)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPR014C(YPR014C_d)|FD-Score:4.63|P-value:1.83E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR099C(YPR099C_d)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:ACB1(YGR037C)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ACS1(YAL054C)|FD-Score:-3.35|P-value:4.04E-4||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:AIM3(YBR108W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:AIR2(YDL175C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:ANS1(YHR126C_p)|FD-Score:-3.38|P-value:3.62E-4||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:AQY1(YPR192W)|FD-Score:3.83|P-value:6.45E-5||SGD DESC:Spore-specific water channel that mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance Gene:ARN1(YHL040C)|FD-Score:4.56|P-value:2.59E-6||SGD DESC:ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress Gene:ASI1(YMR119W)|FD-Score:4.28|P-value:9.27E-6||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ATG8(YBL078C)|FD-Score:-3.82|P-value:6.78E-5||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BDS1(YOL164W)|FD-Score:3.15|P-value:8.14E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BUD19(YJL188C_d)|FD-Score:4.43|P-value:4.67E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUD21(YOR078W)|FD-Score:4.96|P-value:3.55E-7||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:CAN1(YEL063C)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:CAP2(YIL034C)|FD-Score:4.83|P-value:6.93E-7||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CBP6(YBR120C)|FD-Score:-3.86|P-value:5.69E-5||SGD DESC:Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:COA2(YPL189C-A)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:CRP1(YHR146W)|FD-Score:3.45|P-value:2.75E-4||SGD DESC:Protein that binds to cruciform DNA structures Gene:CWC15(YDR163W)|FD-Score:-3.1|P-value:9.67E-4||SGD DESC:Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p Gene:DAL2(YIR029W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DFG5(YMR238W)|FD-Score:4.42|P-value:5.02E-6||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DIA2(YOR080W)|FD-Score:5.03|P-value:2.49E-7||SGD DESC:Origin-binding F-box protein; forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication; plays a role in transcription; required for correct assembly of RSC complex, correct RSC-mediated transcription regulation, and correct nucleosome positioning; involved in invasive and pseudohyphal growth; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:DOG2(YHR043C)|FD-Score:-5.23|P-value:8.57E-8||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:DTR1(YBR180W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:DUS1(YML080W)|FD-Score:4.81|P-value:7.46E-7||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17 Gene:ECM23(YPL021W)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p Gene:ECM34(YHL043W)|FD-Score:3.14|P-value:8.58E-4||SGD DESC:Putative protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:EGT2(YNL327W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner Gene:ERJ5(YFR041C)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:FAA1(YOR317W)|FD-Score:-3.28|P-value:5.14E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; accounts for most acyl-CoA synthetase activity; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms ER foci upon DNA replication stress; FAA1 has a paralog, FAA4, that arose from the whole genome duplication Gene:FAA3(YIL009W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:FYV4(YHR059W)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCY1(YOR120W)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GLE2(YER107C)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GRE2(YOL151W)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:GRR1(YJR090C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GTR2(YGR163W)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GZF3(YJL110C)|FD-Score:-5.76|P-value:4.12E-9||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HCM1(YCR065W)|FD-Score:5.07|P-value:1.96E-7||SGD DESC:Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role Gene:HPA3(YEL066W)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro Gene:HSL7(YBR133C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:HYR1(YIR037W)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:IKI1(YHR187W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IML3(YBR107C)|FD-Score:5.84|P-value:2.64E-9||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:ISM1(YPL040C)|FD-Score:5.34|P-value:4.57E-8||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:JJJ1(YNL227C)|FD-Score:4.82|P-value:7.02E-7||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:KTR7(YIL085C)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication Gene:LIN1(YHR156C)|FD-Score:5.56|P-value:1.35E-8||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:MNN11(YJL183W)|FD-Score:6.95|P-value:1.82E-12||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MON2(YNL297C)|FD-Score:-5.66|P-value:7.45E-9||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:MPD2(YOL088C)|FD-Score:-7.9|P-value:1.44E-15||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRPS28(YDR337W)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSC7(YHR039C)|FD-Score:3.99|P-value:3.27E-5||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MTQ1(YNL063W)|FD-Score:-4.04|P-value:2.72E-5||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene Gene:NIF3(YGL221C)|FD-Score:5.41|P-value:3.18E-8||SGD DESC:Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PFA3(YNL326C)|FD-Score:4.82|P-value:7.00E-7||SGD DESC:Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions Gene:PUS7(YOR243C)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:PYC1(YGL062W)|FD-Score:-3.36|P-value:3.93E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:QDR3(YBR043C)|FD-Score:3.73|P-value:9.43E-5||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:RAD30(YDR419W)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV Gene:RIF2(YLR453C)|FD-Score:4.86|P-value:5.77E-7||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:ROT2(YBR229C)|FD-Score:5.68|P-value:6.82E-9||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:SAP4(YGL229C)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SEC66(YBR171W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SEE1(YIL064W)|FD-Score:-4.44|P-value:4.54E-6||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SIS2(YKR072C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLM6(YBR266C_d)|FD-Score:6.13|P-value:4.34E-10||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SNQ2(YDR011W)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPG5(YMR191W)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SWI4(YER111C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:SYH1(YPL105C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TRM1(YDR120C)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:UBC11(YOR339C)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:UBC4(YBR082C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication Gene:UBP11(YKR098C)|FD-Score:3.75|P-value:8.91E-5||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins Gene:URA2(YJL130C)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:USV1(YPL230W)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Putative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in growth on non-fermentable carbon sources, response to salt stress and cell wall biosynthesis; USV1 has a paralog, RGM1, that arose from the whole genome duplication Gene:YAR023C(YAR023C_p)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family Gene:YBL100W-C(YBL100W-C_p)|FD-Score:-3.82|P-value:6.60E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR023C(YCR023C)|FD-Score:-3.18|P-value:7.49E-4||SGD DESC:Vacuolar membrane protein of unknown function; member of the multidrug resistance family; YCR023C is not an essential gene Gene:YDR169C-A(YDR169C-A_p)|FD-Score:-5.85|P-value:2.50E-9||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YFH7(YFR007W)|FD-Score:3.98|P-value:3.47E-5||SGD DESC:Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases Gene:YFR032C-B(YFR032C-B_p)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YGL072C(YGL072C_d)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR069W(YGR069W_d)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR086W-A(YHR086W-A_p)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIL092W(YIL092W_p)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YKL075C(YKL075C_p)|FD-Score:-3.17|P-value:7.72E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YLL054C(YLL054C_p)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YLR224W(YLR224W_p)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene Gene:YML007C-A(YML007C-A_p)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML122C(YML122C_d)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR085W(YMR085W_p)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YNL155W(YNL155W_p)|FD-Score:5.78|P-value:3.78E-9||SGD DESC:Protein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOL024W(YOL024W_p)|FD-Score:-4.15|P-value:1.67E-5||SGD DESC:Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication Gene:YOR296W(YOR296W_p)|FD-Score:-3.74|P-value:9.09E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene Gene:YPL039W(YPL039W_p)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPR014C(YPR014C_d)|FD-Score:4.63|P-value:1.83E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR099C(YPR099C_d)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR203W9.095.00E-202.61TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YER003C6.484.64E-110.81PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YKL193C5.677.31E-90.63SDS22Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function
YGR098C5.042.38E-70.47ESP1Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
YGL113W4.572.49E-60.06SLD3Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress
YLR060W4.503.36E-60.01FRS1Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
YMR290W-A_d4.503.47E-60.15YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YMR059W4.356.85E-60.01SEN15Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p
YHR062C4.347.14E-60.02RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YER136W4.327.68E-60.04GDI1GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
YHR007C4.289.17E-60.47ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YKL145W3.816.98E-50.03RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YMR049C3.787.78E-50.08ERB1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1
YGR116W3.701.07E-40.03SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
YEL035C_p3.671.20E-40.01UTR5_pProtein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL183W6.951.82E-12MNN11Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
YBR266C_d6.134.34E-10SLM6_dProtein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase
YBR107C5.842.64E-9IML3Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1
YNL155W_p5.783.78E-9YNL155W_pProtein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress
YBR229C5.686.82E-9ROT2Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
YHR156C5.561.35E-8LIN1Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication
YGL221C5.413.18E-8NIF3Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YPL040C5.344.57E-8ISM1Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
YCR065W5.071.96E-7HCM1Forkhead transcription factor that drives S-phase specific expression of genes involved in chromosome segregation, spindle dynamics, and budding; suppressor of calmodulin mutants with specific SPB assembly defects; telomere maintenance role
YOR080W5.032.49E-7DIA2Origin-binding F-box protein; forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication; plays a role in transcription; required for correct assembly of RSC complex, correct RSC-mediated transcription regulation, and correct nucleosome positioning; involved in invasive and pseudohyphal growth; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
YOR078W4.963.55E-7BUD21Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern
YLR453C4.865.77E-7RIF2Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation
YIL034C4.836.93E-7CAP2Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
YNL326C4.827.00E-7PFA3Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions
YNL227C4.827.02E-7JJJ1Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis

GO enrichment analysis for SGTC_911
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1286.15E-23SGTC_2727riluzole 51.8 μMMiscellaneous50700.107692mitochondrial processes
0.1062.26E-16SGTC_8510394-0702 453.0 μMChemDiv (Drug-like library)7626460.0724638mitochondrial processes
0.0904.75E-12SGTC_1771327-0068 8.4 μMChemDiv (Drug-like library)153899000.169231
0.0822.75E-10SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.08RSC & ERG11
0.0814.90E-10SGTC_2519cryptotanshinone 88.0 μMMicrosource (Natural product library)1602540.0909091heme biosynthesis & mitochondrial translocase
0.0791.07E-9SGTC_31129123419 49.5 μMChembridge (Drug-like library)170158540.1RPP1 & pyrimidine depletion
0.0764.38E-9SGTC_10164226-1401 38.0 μMChemDiv (Drug-like library)29016130.135802RNA pol III & RNase P/MRP
0.0765.05E-9SGTC_1641st009299 47.1 μMTimTec (Natural product derivative library)40460540.0810811mitochondrial processes
0.0741.20E-8SGTC_2676harmalol 100.0 μMMicrosource (Natural product library)54591870.0714286mitochondrial processes
0.0723.56E-8SGTC_1073triclabendazole 3.1 μMNIH Clinical Collection502480.0945946
0.0706.07E-8SGTC_1567st069303 68.4 μMTimTec (Pure natural product library)55813190.139241mitochondrial processes
0.0707.10E-8SGTC_12380333-0287 48.8 μMChemDiv (Drug-like library)72260.0645161Golgi
0.0691.02E-7SGTC_2129girgensonine 121.1 μMChembridge (Fragment library)4426380.0615385heme biosynthesis & mitochondrial translocase
0.0681.46E-7SGTC_6790422-0091 41.4 μMChemDiv (Drug-like library)6273840.0806452Golgi
0.0681.66E-7SGTC_21996590217 200.0 μMChembridge (Fragment library)7031090.184615mitochondrial processes

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1909st06083751.5 μM0.362069678937TimTec (Natural product derivative library)266.291263.7103
SGTC_1900st06020072.8 μM0.345455688945TimTec (Natural product derivative library)204.221882.67803
SGTC_7323970-172752.7 μM0.3428571275699ChemDiv (Drug-like library)416.4263.87115
SGTC_11534055-0057267 μM0.333333882706ChemDiv (Drug-like library)299.299721.67306
SGTC_1904st06022578 μM0.32142966595TimTec (Natural product derivative library)204.221882.67803
SGTC_15773(2'-chlorophenyl)-7-ethoxycoumarin66.5 μM0.31746689007TimTec (Pure natural product library)300.736324.37503
SGTC_3184910875849.47 μM0.315789902885Chembridge (Drug-like library)241.28512.94712
SGTC_2103551-044556.83 μM0.3043482865180ChemDiv (Drug-like library)371.406983.0316
SGTC_2483572905027.16 μM0.3043482865180Miscellaneous371.406983.0316
SGTC_15956-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin67.5 μM0.301587688788TimTec (Pure natural product library)296.317243.91514