3474-0017

(7-nitro-2,1,3-benzoxadiazol-4-yl) N,N-diethylcarbamodithioate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_912
Screen concentration 2.2 μM
Source ChemDiv (Drug-like library)
PubChem CID 3360550
SMILES CCN(CC)C(=S)SC1=CC=C(C2=NON=C12)[N+](=O)[O-]
Standardized SMILES CCN(CC)C(=S)Sc1ccc(c2nonc12)[N+](=O)[O-]
Molecular weight 312.368
ALogP 3.94
H-bond donor count 0
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.77
% growth inhibition (Hom. pool) 3.66


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3360550
Download HIP data (tab-delimited text)  (excel)
Gene:FCP1(YMR277W)|FD-Score:3.89|P-value:5.07E-5|Clearance:0.04||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:PGA1(YNL158W)|FD-Score:3.58|P-value:1.70E-4|Clearance:0.11||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:PRE5(YMR314W)|FD-Score:3.81|P-value:6.98E-5|Clearance:0.12||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:QRI1(YDL103C)|FD-Score:-3.76|P-value:8.61E-5|Clearance:0||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RPA190(YOR341W)|FD-Score:3.19|P-value:7.12E-4|Clearance:0.01||SGD DESC:RNA polymerase I largest subunit A190 Gene:RPB3(YIL021W)|FD-Score:3.18|P-value:7.47E-4|Clearance:0.07||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPC17(YJL011C)|FD-Score:-3.31|P-value:4.66E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPL15A(YLR029C)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.08||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPN11(YFR004W)|FD-Score:3.42|P-value:3.16E-4|Clearance:0.09||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPN8(YOR261C)|FD-Score:4.15|P-value:1.68E-5|Clearance:0.26||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RPN9(YDR427W)|FD-Score:3.85|P-value:5.89E-5|Clearance:0.04||SGD DESC:Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress Gene:SLU7(YDR088C)|FD-Score:-3.1|P-value:9.63E-4|Clearance:0||SGD DESC:RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain Gene:SUP35(YDR172W)|FD-Score:-3.15|P-value:8.06E-4|Clearance:0||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TFG1(YGR186W)|FD-Score:3.1|P-value:9.54E-4|Clearance:0.02||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:YFH1(YDL120W)|FD-Score:3.33|P-value:4.32E-4|Clearance:0.14||SGD DESC:Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia Gene:YOL134C(YOL134C_d)|FD-Score:-3.5|P-value:2.37E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit Gene:YRA1(YDR381W)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.02||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member Gene:YRB2(YIL063C)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.06||SGD DESC:Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential Gene:FCP1(YMR277W)|FD-Score:3.89|P-value:5.07E-5|Clearance:0.04||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:PGA1(YNL158W)|FD-Score:3.58|P-value:1.70E-4|Clearance:0.11||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:PRE5(YMR314W)|FD-Score:3.81|P-value:6.98E-5|Clearance:0.12||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:QRI1(YDL103C)|FD-Score:-3.76|P-value:8.61E-5|Clearance:0||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RPA190(YOR341W)|FD-Score:3.19|P-value:7.12E-4|Clearance:0.01||SGD DESC:RNA polymerase I largest subunit A190 Gene:RPB3(YIL021W)|FD-Score:3.18|P-value:7.47E-4|Clearance:0.07||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPC17(YJL011C)|FD-Score:-3.31|P-value:4.66E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPL15A(YLR029C)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.08||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPN11(YFR004W)|FD-Score:3.42|P-value:3.16E-4|Clearance:0.09||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:RPN8(YOR261C)|FD-Score:4.15|P-value:1.68E-5|Clearance:0.26||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p Gene:RPN9(YDR427W)|FD-Score:3.85|P-value:5.89E-5|Clearance:0.04||SGD DESC:Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress Gene:SLU7(YDR088C)|FD-Score:-3.1|P-value:9.63E-4|Clearance:0||SGD DESC:RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain Gene:SUP35(YDR172W)|FD-Score:-3.15|P-value:8.06E-4|Clearance:0||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TFG1(YGR186W)|FD-Score:3.1|P-value:9.54E-4|Clearance:0.02||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:YFH1(YDL120W)|FD-Score:3.33|P-value:4.32E-4|Clearance:0.14||SGD DESC:Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia Gene:YOL134C(YOL134C_d)|FD-Score:-3.5|P-value:2.37E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit Gene:YRA1(YDR381W)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.02||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member Gene:YRB2(YIL063C)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.06||SGD DESC:Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3360550
Download HOP data (tab-delimited text)  (excel)
Gene:ACS1(YAL054C)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:ALG9(YNL219C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:APD1(YBR151W)|FD-Score:3.72|P-value:9.93E-5||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:AQY1(YPR192W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Spore-specific water channel that mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance Gene:ARN2(YHL047C)|FD-Score:5.46|P-value:2.37E-8||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:ARO2(YGL148W)|FD-Score:5.99|P-value:1.06E-9||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:BXI1(YNL305C)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p Gene:CAJ1(YER048C)|FD-Score:3.89|P-value:4.93E-5||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CCS1(YMR038C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CHS7(YHR142W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COG1(YGL223C)|FD-Score:-3.94|P-value:4.12E-5||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CST6(YIL036W)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:DGA1(YOR245C)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles Gene:DOM34(YNL001W)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication Gene:DOT1(YDR440W)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:EPT1(YHR123W)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication Gene:FAR7(YFR008W)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FET4(YMR319C)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FKH2(YNL068C)|FD-Score:5.19|P-value:1.07E-7||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FUI1(YBL042C)|FD-Score:4.3|P-value:8.55E-6||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GCN20(YFR009W)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GET2(YER083C)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GLY1(YEL046C)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GRX4(YER174C)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 Gene:GTT3(YEL017W)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:GZF3(YJL110C)|FD-Score:6.65|P-value:1.49E-11||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HTZ1(YOL012C)|FD-Score:5.33|P-value:4.87E-8||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:LIF1(YGL090W)|FD-Score:5.12|P-value:1.53E-7||SGD DESC:Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein Gene:LYS2(YBR115C)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p Gene:MAC1(YMR021C)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAF1(YDR005C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MSB3(YNL293W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSW1(YDR268W)|FD-Score:-3.14|P-value:8.50E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTL1(YGR023W)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Putative plasma membrane sensor, involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p Gene:MTQ1(YNL063W)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene Gene:NPR1(YNL183C)|FD-Score:-3.1|P-value:9.65E-4||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:NVJ1(YHR195W)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:OSM1(YJR051W)|FD-Score:-3.29|P-value:5.06E-4||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PAU13(YHL046C)|FD-Score:3.9|P-value:4.83E-5||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock Gene:PBS2(YJL128C)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PHO81(YGR233C)|FD-Score:5.52|P-value:1.73E-8||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PRD1(YCL057W)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:PTP1(YDL230W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation Gene:REV1(YOR346W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RHO2(YNL090W)|FD-Score:5.21|P-value:9.66E-8||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RIF2(YLR453C)|FD-Score:4.49|P-value:3.59E-6||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:SAC7(YDR389W)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SNF5(YBR289W)|FD-Score:-4.35|P-value:6.94E-6||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SNQ2(YDR011W)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPO7(YAL009W)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SRB8(YCR081W)|FD-Score:4.89|P-value:5.05E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SUC2(YIL162W)|FD-Score:5.6|P-value:1.07E-8||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:SVS1(YPL163C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:SWI6(YLR182W)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:SYM1(YLR251W)|FD-Score:3.79|P-value:7.42E-5||SGD DESC:Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 Gene:TMA7(YLR262C-A)|FD-Score:-5.17|P-value:1.17E-7||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:7.49|P-value:3.48E-14||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UBS1(YBR165W)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity Gene:UFD4(YKL010C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress Gene:URA1(YKL216W)|FD-Score:4.27|P-value:9.93E-6||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VID22(YLR373C)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VMA4(YOR332W)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VPS17(YOR132W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:YAF9(YNL107W)|FD-Score:-4.11|P-value:2.02E-5||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YBR206W(YBR206W_d)|FD-Score:-3.18|P-value:7.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YHB1(YGR234W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YIL029C(YIL029C_p)|FD-Score:-4.28|P-value:9.21E-6||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YIL055C(YIL055C_p)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Putative protein of unknown function Gene:YLR046C(YLR046C_p)|FD-Score:3.79|P-value:7.68E-5||SGD DESC:Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YML122C(YML122C_d)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR304C-A(YMR304C-A_d)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YOL085C(YOL085C_d)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Gene:YOR214C(YOR214C_p)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Putative protein of unknown function; YOR214C is not an essential gene Gene:YOR304C-A(YOR304C-A_p)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YOR365C(YOR365C_p)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication Gene:YOR376W(YOR376W_d)|FD-Score:4.86|P-value:6.01E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene. Gene:YPR076W(YPR076W_d)|FD-Score:-4.37|P-value:6.12E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR099C(YPR099C_d)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:YPR123C(YPR123C_d)|FD-Score:-4.07|P-value:2.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR159C-A(YPR159C-A_p)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPT52(YKR014C)|FD-Score:5.33|P-value:4.90E-8||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:ACS1(YAL054C)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:ALG9(YNL219C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:APD1(YBR151W)|FD-Score:3.72|P-value:9.93E-5||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:AQY1(YPR192W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Spore-specific water channel that mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance Gene:ARN2(YHL047C)|FD-Score:5.46|P-value:2.37E-8||SGD DESC:Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C Gene:ARO2(YGL148W)|FD-Score:5.99|P-value:1.06E-9||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:BXI1(YNL305C)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p Gene:CAJ1(YER048C)|FD-Score:3.89|P-value:4.93E-5||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CCS1(YMR038C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CHS7(YHR142W)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COG1(YGL223C)|FD-Score:-3.94|P-value:4.12E-5||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CST6(YIL036W)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:DGA1(YOR245C)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles Gene:DOM34(YNL001W)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication Gene:DOT1(YDR440W)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:EPT1(YHR123W)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication Gene:FAR7(YFR008W)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FET4(YMR319C)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FKH2(YNL068C)|FD-Score:5.19|P-value:1.07E-7||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FUI1(YBL042C)|FD-Score:4.3|P-value:8.55E-6||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GCN20(YFR009W)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GET2(YER083C)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GLY1(YEL046C)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GRX4(YER174C)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 Gene:GTT3(YEL017W)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:GZF3(YJL110C)|FD-Score:6.65|P-value:1.49E-11||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HTZ1(YOL012C)|FD-Score:5.33|P-value:4.87E-8||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:LIF1(YGL090W)|FD-Score:5.12|P-value:1.53E-7||SGD DESC:Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein Gene:LYS2(YBR115C)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p Gene:MAC1(YMR021C)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAF1(YDR005C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MSB3(YNL293W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSW1(YDR268W)|FD-Score:-3.14|P-value:8.50E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTL1(YGR023W)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Putative plasma membrane sensor, involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p Gene:MTQ1(YNL063W)|FD-Score:4.23|P-value:1.17E-5||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene Gene:NPR1(YNL183C)|FD-Score:-3.1|P-value:9.65E-4||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:NVJ1(YHR195W)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:OSM1(YJR051W)|FD-Score:-3.29|P-value:5.06E-4||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PAU13(YHL046C)|FD-Score:3.9|P-value:4.83E-5||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock Gene:PBS2(YJL128C)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PHO81(YGR233C)|FD-Score:5.52|P-value:1.73E-8||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PRD1(YCL057W)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress Gene:PTP1(YDL230W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation Gene:REV1(YOR346W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RHO2(YNL090W)|FD-Score:5.21|P-value:9.66E-8||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RIF2(YLR453C)|FD-Score:4.49|P-value:3.59E-6||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:SAC7(YDR389W)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SNF5(YBR289W)|FD-Score:-4.35|P-value:6.94E-6||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SNQ2(YDR011W)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPO7(YAL009W)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SRB8(YCR081W)|FD-Score:4.89|P-value:5.05E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SUC2(YIL162W)|FD-Score:5.6|P-value:1.07E-8||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:SVS1(YPL163C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:SWI6(YLR182W)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:SYM1(YLR251W)|FD-Score:3.79|P-value:7.42E-5||SGD DESC:Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 Gene:TMA7(YLR262C-A)|FD-Score:-5.17|P-value:1.17E-7||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:7.49|P-value:3.48E-14||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UBS1(YBR165W)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity Gene:UFD4(YKL010C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress Gene:URA1(YKL216W)|FD-Score:4.27|P-value:9.93E-6||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VID22(YLR373C)|FD-Score:-3.42|P-value:3.09E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VMA4(YOR332W)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VPS17(YOR132W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:YAF9(YNL107W)|FD-Score:-4.11|P-value:2.02E-5||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YBR206W(YBR206W_d)|FD-Score:-3.18|P-value:7.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YHB1(YGR234W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YIL029C(YIL029C_p)|FD-Score:-4.28|P-value:9.21E-6||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YIL055C(YIL055C_p)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Putative protein of unknown function Gene:YLR046C(YLR046C_p)|FD-Score:3.79|P-value:7.68E-5||SGD DESC:Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YML122C(YML122C_d)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR304C-A(YMR304C-A_d)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YOL085C(YOL085C_d)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Gene:YOR214C(YOR214C_p)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Putative protein of unknown function; YOR214C is not an essential gene Gene:YOR304C-A(YOR304C-A_p)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YOR365C(YOR365C_p)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Putative protein of unknown function; not an essential protein; YOR365C has a paralog, FLC2, that arose from the whole genome duplication Gene:YOR376W(YOR376W_d)|FD-Score:4.86|P-value:6.01E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene. Gene:YPR076W(YPR076W_d)|FD-Score:-4.37|P-value:6.12E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR099C(YPR099C_d)|FD-Score:-3.09|P-value:9.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:YPR123C(YPR123C_d)|FD-Score:-4.07|P-value:2.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR159C-A(YPR159C-A_p)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPT52(YKR014C)|FD-Score:5.33|P-value:4.90E-8||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR261C4.151.68E-50.26RPN8Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p
YMR277W3.895.07E-50.04FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YDR427W3.855.89E-50.04RPN9Non-ATPase regulatory subunit of the 26S proteasome; has similarity to putative proteasomal subunits in other species; null mutant is temperature sensitive and exhibits cell cycle and proteasome assembly defects; protein abundance increases in response to DNA replication stress
YMR314W3.816.98E-50.12PRE5Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YLR029C3.691.13E-40.08RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YDR381W3.611.54E-40.02YRA1RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member
YNL158W3.581.70E-40.11PGA1Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes
YIL063C3.482.53E-40.06YRB2Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential
YFR004W3.423.16E-40.09RPN11Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
YDL120W3.334.32E-40.14YFH1Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia
YOR341W3.197.12E-40.01RPA190RNA polymerase I largest subunit A190
YIL021W3.187.47E-40.07RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
YGR186W3.109.54E-40.02TFG1TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74
YOL038W3.090.001020.04PRE6Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
YBR247C3.040.001170.05ENP1Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR007W7.493.48E-14TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YJL110C6.651.49E-11GZF3GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication
YGL148W5.991.06E-9ARO2Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress
YIL162W5.601.07E-8SUC2Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively
YGR233C5.521.73E-8PHO81Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress
YHL047C5.462.37E-8ARN2Transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C
YOL012C5.334.87E-8HTZ1Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin
YKR014C5.334.90E-8YPT52Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress
YNL090W5.219.66E-8RHO2Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly
YNL068C5.191.07E-7FKH2Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication
YGL090W5.121.53E-7LIF1Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein
YCR081W4.895.05E-7SRB8Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
YOR376W_d4.866.01E-7YOR376W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene.
YLR453C4.493.59E-6RIF2Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation
YBL042C4.308.55E-6FUI1High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress

GO enrichment analysis for SGTC_912
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1201.71E-20SGTC_8191013-0258 27.7 μMChemDiv (Drug-like library)57531980.0864198
0.1025.44E-15SGTC_1715st032160 10.2 μMTimTec (Natural product derivative library)52898250.0574713
0.0944.52E-13SGTC_305-(2-thienylidene)-Rhodanine 28.7 μMChemDiv (Drug-like library)12411320.0735294
0.0903.67E-12SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.104478
0.0896.48E-12SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.0714286
0.0872.64E-11SGTC_18825615643 20.0 μMMiscellaneous22530750.0833333TRP & mitochondrial translation
0.0864.49E-11SGTC_9811488-1094 54.9 μMChemDiv (Drug-like library)34495220.0823529
0.0815.17E-10SGTC_2524avocatin b 10.6 μMMicrosource (Natural product library)67107480.0153846
0.0807.34E-10SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0394737
0.0791.02E-9SGTC_2540epiandrosterone 100.0 μMTimTec (Pure natural product library)4413020.0394737
0.0791.04E-9SGTC_1999st074831 12.5 μMTimTec (Natural product derivative library)171125230.0897436plasma membrane duress
0.0791.11E-9SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.0704225
0.0781.65E-9SGTC_478N-arachidonylglycine 10.0 μMICCB bioactive library52833890.0724638
0.0772.75E-9SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.0561798
0.0773.22E-9SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0963855

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_8201582-006059.9 μM0.3684213848840ChemDiv (Drug-like library)290.661983.50816
SGTC_8151582-002736.9 μM0.3548393128206ChemDiv (Drug-like library)286.24292.82717
SGTC_720r052-164449.9 μM0.3064521422863ChemDiv (Drug-like library)289.652461.98118TSC3-RPN4
SGTC_1063disulfiram1.84 μM0.2954553117NIH Clinical Collection296.53925.604
SGTC_2759disulfiram3.38 μM0.2954553117Miscellaneous296.53925.604
SGTC_244nsc-2078951.3 μM0.2812542640Miscellaneous279.25202-1.27707DNA damage response
SGTC_890302-0250142.11 μM0.2727274203518ChemDiv (Drug-like library)255.227322.23616
SGTC_13591582-0056520 nM0.2615383661189ChemDiv (Drug-like library)304.688563.99416
SGTC_7342023-041780 μM0.2461543935732ChemDiv (Drug-like library)264.237381.79117ergosterol biosynthesis
SGTC_10004048-08848.67 μM0.24615413040088ChemDiv (Drug-like library)287.249261.60508copper-dependent oxidative stress
SGTC_22727805186180 μM0.2419352956062Chembridge (Fragment library)268.335342.54524cell wall signaling