0063-0024

2,2,6,6-tetramethyl-1-(2,2,2-trichloro-1-hydroxyethyl)piperidin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_92
Screen concentration 660.9 μM
Source ChemDiv (Drug-like library)
PubChem CID 3594437
SMILES CC1(CC(=O)CC(N1C(C(Cl)(Cl)Cl)O)(C)C)C
Standardized SMILES CC1(C)CC(=O)CC(C)(C)N1C(O)C(Cl)(Cl)Cl
Molecular weight 302.6251
ALogP 2.51
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.21
% growth inhibition (Hom. pool) -0.02


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3594437
Download HIP data (tab-delimited text)  (excel)
Gene:ERG10(YPL028W)|FD-Score:-3.35|P-value:4.07E-4|Clearance:0||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:MAK21(YDR060W)|FD-Score:3.43|P-value:3.01E-4|Clearance:0.03||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:RPS15(YOL040C)|FD-Score:3.24|P-value:6.01E-4|Clearance:0.05||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RTT105(YER104W)|FD-Score:3.4|P-value:3.40E-4|Clearance:0.16||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SDH3(YKL141W)|FD-Score:-5.94|P-value:1.38E-9|Clearance:0||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SPC97(YHR172W)|FD-Score:3.83|P-value:6.52E-5|Clearance:0.23||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TTI1(YKL033W)|FD-Score:3.19|P-value:7.11E-4|Clearance:0.21||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies Gene:YIP1(YGR172C)|FD-Score:-3.56|P-value:1.84E-4|Clearance:0||SGD DESC:Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4 Gene:YJU2(YKL095W)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.17||SGD DESC:Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing Gene:ERG10(YPL028W)|FD-Score:-3.35|P-value:4.07E-4|Clearance:0||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:MAK21(YDR060W)|FD-Score:3.43|P-value:3.01E-4|Clearance:0.03||SGD DESC:Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein Gene:RPS15(YOL040C)|FD-Score:3.24|P-value:6.01E-4|Clearance:0.05||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RTT105(YER104W)|FD-Score:3.4|P-value:3.40E-4|Clearance:0.16||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SDH3(YKL141W)|FD-Score:-5.94|P-value:1.38E-9|Clearance:0||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SPC97(YHR172W)|FD-Score:3.83|P-value:6.52E-5|Clearance:0.23||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TTI1(YKL033W)|FD-Score:3.19|P-value:7.11E-4|Clearance:0.21||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies Gene:YIP1(YGR172C)|FD-Score:-3.56|P-value:1.84E-4|Clearance:0||SGD DESC:Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4 Gene:YJU2(YKL095W)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.17||SGD DESC:Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3594437
Download HOP data (tab-delimited text)  (excel)
Gene:ADA2(YDR448W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:AEP1(YMR064W)|FD-Score:-3.79|P-value:7.63E-5||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:BER1(YLR412W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BPH1(YCR032W)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:CHS5(YLR330W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CMK2(YOL016C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication Gene:DOA4(YDR069C)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:FET4(YMR319C)|FD-Score:3.75|P-value:8.88E-5||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GRE3(YHR104W)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:HOR7(YMR251W-A)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HPM1(YIL110W)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:JEM1(YJL073W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:LEU9(YOR108W)|FD-Score:4.45|P-value:4.20E-6||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:LYP1(YNL268W)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MNE1(YOR350C)|FD-Score:-3.97|P-value:3.65E-5||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MTG1(YMR097C)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NUP84(YDL116W)|FD-Score:3.77|P-value:8.10E-5||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:PAU5(YFL020C)|FD-Score:-3.78|P-value:7.78E-5||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme Gene:PBP2(YBR233W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress Gene:PCL8(YPL219W)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PDR12(YPL058C)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity Gene:PEX12(YMR026C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PHO87(YCR037C)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PRM2(YIL037C)|FD-Score:6.31|P-value:1.43E-10||SGD DESC:Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion Gene:RAD23(YEL037C)|FD-Score:-3.28|P-value:5.21E-4||SGD DESC:Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover Gene:RGI2(YIL057C)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Protein of unknown function involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose Gene:ROY1(YMR258C)|FD-Score:4|P-value:3.19E-5||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPL16A(YIL133C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RTT107(YHR154W)|FD-Score:4.85|P-value:6.05E-7||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SMK1(YPR054W)|FD-Score:4.29|P-value:8.79E-6||SGD DESC:Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p Gene:SPH1(YLR313C)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SRL3(YKR091W)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate Gene:SRO77(YBL106C)|FD-Score:3.81|P-value:6.89E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SYH1(YPL105C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TAT2(YOL020W)|FD-Score:-3.23|P-value:6.17E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TIP41(YPR040W)|FD-Score:5.81|P-value:3.13E-9||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:UBR1(YGR184C)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:YDR344C(YDR344C_d)|FD-Score:-3.33|P-value:4.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR524C-B(YDR524C-B_p)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YGR228W(YGR228W_d)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHR078W(YHR078W_p)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:High osmolarity-regulated gene of unknown function Gene:YIL028W(YIL028W_d)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIR021W-A(YIR021W-A_p)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YML047W-A(YML047W-A_d)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR187C(YMR187C_p)|FD-Score:3.75|P-value:8.96E-5||SGD DESC:Putative protein of unknown function; YMR187C is not an essential gene Gene:YNL226W(YNL226W_d)|FD-Score:3.97|P-value:3.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YPL229W(YPL229W_p)|FD-Score:4.08|P-value:2.30E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:YPR076W(YPR076W_d)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ADA2(YDR448W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:AEP1(YMR064W)|FD-Score:-3.79|P-value:7.63E-5||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:BER1(YLR412W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BPH1(YCR032W)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:CHS5(YLR330W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CMK2(YOL016C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication Gene:DOA4(YDR069C)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:FET4(YMR319C)|FD-Score:3.75|P-value:8.88E-5||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GRE3(YHR104W)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:HOR7(YMR251W-A)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HPM1(YIL110W)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:JEM1(YJL073W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:LEU9(YOR108W)|FD-Score:4.45|P-value:4.20E-6||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:LYP1(YNL268W)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MNE1(YOR350C)|FD-Score:-3.97|P-value:3.65E-5||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MTG1(YMR097C)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:NUP84(YDL116W)|FD-Score:3.77|P-value:8.10E-5||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:PAU5(YFL020C)|FD-Score:-3.78|P-value:7.78E-5||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme Gene:PBP2(YBR233W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress Gene:PCL8(YPL219W)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PDR12(YPL058C)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity Gene:PEX12(YMR026C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PHO87(YCR037C)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PRM2(YIL037C)|FD-Score:6.31|P-value:1.43E-10||SGD DESC:Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion Gene:RAD23(YEL037C)|FD-Score:-3.28|P-value:5.21E-4||SGD DESC:Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover Gene:RGI2(YIL057C)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Protein of unknown function involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose Gene:ROY1(YMR258C)|FD-Score:4|P-value:3.19E-5||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPL16A(YIL133C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RTT107(YHR154W)|FD-Score:4.85|P-value:6.05E-7||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SMK1(YPR054W)|FD-Score:4.29|P-value:8.79E-6||SGD DESC:Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p Gene:SPH1(YLR313C)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SRL3(YKR091W)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate Gene:SRO77(YBL106C)|FD-Score:3.81|P-value:6.89E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SYH1(YPL105C)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TAT2(YOL020W)|FD-Score:-3.23|P-value:6.17E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TIP41(YPR040W)|FD-Score:5.81|P-value:3.13E-9||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:UBR1(YGR184C)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:YDR344C(YDR344C_d)|FD-Score:-3.33|P-value:4.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR524C-B(YDR524C-B_p)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YGR228W(YGR228W_d)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHR078W(YHR078W_p)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:High osmolarity-regulated gene of unknown function Gene:YIL028W(YIL028W_d)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIR021W-A(YIR021W-A_p)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YML047W-A(YML047W-A_d)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR187C(YMR187C_p)|FD-Score:3.75|P-value:8.96E-5||SGD DESC:Putative protein of unknown function; YMR187C is not an essential gene Gene:YNL226W(YNL226W_d)|FD-Score:3.97|P-value:3.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YPL229W(YPL229W_p)|FD-Score:4.08|P-value:2.30E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:YPR076W(YPR076W_d)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR172W3.836.52E-50.23SPC97Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YKL095W3.601.59E-40.17YJU2Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing
YDR060W3.433.01E-40.03MAK21Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
YER104W3.403.40E-40.16RTT105Protein with a role in regulation of Ty1 transposition
YOL040C3.246.01E-40.05RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YKL033W3.197.11E-40.20TTI1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies
YMR208W2.980.001420.15ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YER157W2.840.002260.01COG3Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YHR197W2.830.002350.07RIX1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene
YGR156W2.750.002940.09PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YGR274C2.660.003890.07TAF1TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression
YOR278W2.600.004720.01HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
YDL058W2.590.004840.06USO1Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
YNL317W2.530.005690.01PFS2Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
YPR168W2.520.005850.02NUT2Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIL037C6.311.43E-10PRM2Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion
YPR040W5.813.13E-9TIP41Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress
YHR154W4.856.05E-7RTT107Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress
YOR108W4.454.20E-6LEU9Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication
YPR054W4.298.79E-6SMK1Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p
YPR076W_d4.251.05E-5YPR076W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGR228W_d4.231.18E-5YGR228W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C
YLR313C4.111.94E-5SPH1Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p
YCR032W4.092.20E-5BPH1Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting
YPL229W_p4.082.30E-5YPL229W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication
YMR258C4.003.19E-5ROY1GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p
YNL226W_d3.973.60E-5YNL226W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C
YCR037C3.963.69E-5PHO87Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication
YBL106C3.816.89E-5SRO77Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication
YDL116W3.778.10E-5NUP84Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107

GO enrichment analysis for SGTC_92
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1195.48E-20SGTC_940334-0113 514.7 μMChemDiv (Drug-like library)67951780.0571429
0.0722.56E-8SGTC_980074-0023 107.7 μMChemDiv (Drug-like library)35638210.0740741Golgi
0.0664.44E-7SGTC_950471-0001 561.1 μMChemDiv (Drug-like library)40154050.0892857
0.0648.57E-7SGTC_1164k060-0068 93.7 μMChemDiv (Drug-like library)59636760.0441176
0.0604.69E-6SGTC_8670518-0118 155.0 μMChemDiv (Drug-like library)68481580.0526316DNA intercalators
0.0587.09E-6SGTC_2247cerulenin 830.0 nMICCB bioactive library52820540.0357143
0.0588.32E-6SGTC_233rapamycin 362.0 pMMiscellaneous52846160.0756303
0.0571.40E-5SGTC_25005-alpha-androstan-3,17-dione 61.4 μMMicrosource (Natural product library)67106550.148148
0.0552.68E-5SGTC_2595hexamethylquercetagetin 100.0 μMMicrosource (Natural product library)3863310.0655738
0.0535.06E-5SGTC_1137ixabepilone 100.0 μMMiscellaneous1542250.0344828
0.0526.70E-5SGTC_1517deoxycholic acid methyl ester 49.2 μMTimTec (Pure natural product library)2293460.0972222
0.0527.52E-5SGTC_29067926292 26.4 μMChembridge (Drug-like library)29663190.016129
0.0518.22E-5SGTC_9781416-0512 87.4 μMChemDiv (Drug-like library)68140030.0645161redox potentiating
0.0519.16E-5SGTC_1310141-0277 33.8 μMChemDiv (Drug-like library)15477710.0847458
0.0501.27E-4SGTC_3783970-0959 8.3 μMChemDiv (Drug-like library)66156530.1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_451chloral hydrate100 μM0.252707Miscellaneous165.403020.89922
SGTC_1400062-0056502.53 μM0.23758964ChemDiv (Drug-like library)302.969382.9622
SGTC_12360330-0013258 μM0.24617066ChemDiv (Drug-like library)262.347381.96212
SGTC_2405hr heat shock (37°C) + Streptovitacin300 μM0.18518591467Miscellaneous297.34682-0.35135heat shock/prefoldin
SGTC_421streptovitacin30 mM0.18518591467Miscellaneous297.34682-0.35135
SGTC_460329-0187a2.2 μM0.1846494817ChemDiv (Drug-like library)415.322444.79812
SGTC_14984478-355597.6 μM0.1754393437919ChemDiv (Drug-like library)388.520323.115
SGTC_2415hr heat shock (37°C) + cantharidin155.45 μM0.1752545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_2489cantharidin73.42 μM0.1752545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_408cantharidin100 μM0.1752545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_422cantharidin155 μM0.1752545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_1772st04843982.2 μM0.1730772927068TimTec (Natural product derivative library)243.300982.72702