1561-0023

2-(2-methyl-5-nitroimidazol-1-yl)ethyl N-(3,5-dichlorophenyl)carbamate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_921
Screen concentration 372.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 24050882
SMILES CC1=NC=C(N1CCOC(=O)NC2=CC(=CC(=C2)Cl)Cl)[N+](=O)[O-]
Standardized SMILES Cc1ncc([N+](=O)[O-])n1CCOC(=O)Nc2cc(Cl)cc(Cl)c2
Molecular weight 359.1648
ALogP 2.94
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 21.03
% growth inhibition (Hom. pool) 4.67


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 24050882
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:3.2|P-value:6.77E-4|Clearance:0.01||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ACS2(YLR153C)|FD-Score:3.65|P-value:1.34E-4|Clearance:0||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:ARP7(YPR034W)|FD-Score:4.46|P-value:4.14E-6|Clearance:0.46||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRN1(YBL097W)|FD-Score:3.19|P-value:7.08E-4|Clearance:0.07||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CDC19(YAL038W)|FD-Score:-4.43|P-value:4.77E-6|Clearance:0||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:DSL1(YNL258C)|FD-Score:-3.65|P-value:1.32E-4|Clearance:0||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:FBA1(YKL060C)|FD-Score:-3.39|P-value:3.56E-4|Clearance:0||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:HEM12(YDR047W)|FD-Score:3.84|P-value:6.15E-5|Clearance:0.12||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:NOP15(YNL110C)|FD-Score:4.75|P-value:1.04E-6|Clearance:0.29||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NOP8(YOL144W)|FD-Score:7.42|P-value:5.80E-14|Clearance:1.07||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PAM16(YJL104W)|FD-Score:-3.91|P-value:4.53E-5|Clearance:0||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain Gene:PGS1(YCL004W)|FD-Score:-3.37|P-value:3.81E-4|Clearance:0||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PKC1(YBL105C)|FD-Score:5.33|P-value:4.96E-8|Clearance:0.58||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:POL1(YNL102W)|FD-Score:3.12|P-value:9.13E-4|Clearance:0.15||SGD DESC:Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis Gene:RLP24(YLR009W)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.04||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPB10(YOR210W)|FD-Score:3.3|P-value:4.83E-4|Clearance:0.04||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPL10(YLR075W)|FD-Score:3.26|P-value:5.54E-4|Clearance:0.06||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RSC6(YCR052W)|FD-Score:4|P-value:3.22E-5|Clearance:0.16||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SPN1(YPR133C)|FD-Score:3.72|P-value:9.81E-5|Clearance:0.08||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TAM41(YGR046W)|FD-Score:6.35|P-value:1.06E-10|Clearance:1.02||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:UBA1(YKL210W)|FD-Score:-3.19|P-value:7.08E-4|Clearance:0||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:YPL238C(YPL238C_d)|FD-Score:3.61|P-value:1.56E-4|Clearance:0.3||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:ACC1(YNR016C)|FD-Score:3.2|P-value:6.77E-4|Clearance:0.01||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ACS2(YLR153C)|FD-Score:3.65|P-value:1.34E-4|Clearance:0||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:ARP7(YPR034W)|FD-Score:4.46|P-value:4.14E-6|Clearance:0.46||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRN1(YBL097W)|FD-Score:3.19|P-value:7.08E-4|Clearance:0.07||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CDC19(YAL038W)|FD-Score:-4.43|P-value:4.77E-6|Clearance:0||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:DSL1(YNL258C)|FD-Score:-3.65|P-value:1.32E-4|Clearance:0||SGD DESC:Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p and Tip20p that interacts with ER SNAREs Sec20p and Use1p; component of the ER target site that interacts with coatomer; interacts with Cin5p; similar to the fly and human ZW10 gene Gene:FBA1(YKL060C)|FD-Score:-3.39|P-value:3.56E-4|Clearance:0||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:HEM12(YDR047W)|FD-Score:3.84|P-value:6.15E-5|Clearance:0.12||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:NOP15(YNL110C)|FD-Score:4.75|P-value:1.04E-6|Clearance:0.29||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NOP8(YOL144W)|FD-Score:7.42|P-value:5.80E-14|Clearance:1.07||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PAM16(YJL104W)|FD-Score:-3.91|P-value:4.53E-5|Clearance:0||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain Gene:PGS1(YCL004W)|FD-Score:-3.37|P-value:3.81E-4|Clearance:0||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PKC1(YBL105C)|FD-Score:5.33|P-value:4.96E-8|Clearance:0.58||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:POL1(YNL102W)|FD-Score:3.12|P-value:9.13E-4|Clearance:0.15||SGD DESC:Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis Gene:RLP24(YLR009W)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.04||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPB10(YOR210W)|FD-Score:3.3|P-value:4.83E-4|Clearance:0.04||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPL10(YLR075W)|FD-Score:3.26|P-value:5.54E-4|Clearance:0.06||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RSC6(YCR052W)|FD-Score:4|P-value:3.22E-5|Clearance:0.16||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SPN1(YPR133C)|FD-Score:3.72|P-value:9.81E-5|Clearance:0.08||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TAM41(YGR046W)|FD-Score:6.35|P-value:1.06E-10|Clearance:1.02||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:UBA1(YKL210W)|FD-Score:-3.19|P-value:7.08E-4|Clearance:0||SGD DESC:Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress Gene:YPL238C(YPL238C_d)|FD-Score:3.61|P-value:1.56E-4|Clearance:0.3||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 24050882
Download HOP data (tab-delimited text)  (excel)
Gene:ADE8(YDR408C)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ARG82(YDR173C)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ART5(YGR068C)|FD-Score:-3.78|P-value:7.74E-5||SGD DESC:Protein proposed to regulate the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane Gene:BIO4(YNR057C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BUD27(YFL023W)|FD-Score:4.99|P-value:2.98E-7||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CCR4(YAL021C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CCZ1(YBR131W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway Gene:COX7(YMR256C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CPR5(YDR304C)|FD-Score:3.77|P-value:8.02E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:DNM1(YLL001W)|FD-Score:-3.47|P-value:2.65E-4||SGD DESC:Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance Gene:ECM5(YMR176W)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:EMC2(YJR088C)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 Gene:EMC6(YLL014W)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:ENT4(YLL038C)|FD-Score:-4.02|P-value:2.97E-5||SGD DESC:Protein of unknown function, contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome Gene:ERG2(YMR202W)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERS1(YCR075C)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains Gene:FLC1(YPL221W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance Gene:GET2(YER083C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:HIT1(YJR055W)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HSM3(YBR272C)|FD-Score:5.24|P-value:8.05E-8||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:HSP82(YPL240C)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:IDS2(YJL146W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation Gene:IMP2'(YIL154C)|FD-Score:4.35|P-value:6.80E-6||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:MEP1(YGR121C)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MNE1(YOR350C)|FD-Score:-3.24|P-value:5.97E-4||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MTF2(YDL044C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:MYO4(YAL029C)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:NRM1(YNR009W)|FD-Score:5.33|P-value:5.04E-8||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:OSW5(YMR148W)|FD-Score:5.27|P-value:6.72E-8||SGD DESC:Protein of unknown function that may play a role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion Gene:PAL1(YDR348C)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PES4(YFR023W)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PRY2(YKR013W)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:PTP3(YER075C)|FD-Score:-5.16|P-value:1.27E-7||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Gene:PUS7(YOR243C)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RIP1(YEL024W)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:ROT2(YBR229C)|FD-Score:-4.03|P-value:2.81E-5||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:ROX1(YPR065W)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RPE1(YJL121C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL18B(YNL301C)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPL27A(YHR010W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RRD2(YPL152W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RTC1(YOL138C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:RTS3(YGR161C_p)|FD-Score:4.61|P-value:2.05E-6||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:RTT106(YNL206C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SKT5(YBL061C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SNF6(YHL025W)|FD-Score:-5.27|P-value:6.95E-8||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SPO20(YMR017W)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog Gene:SRB2(YHR041C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SSH1(YBR283C)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:SVF1(YDR346C)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:TED1(YIL039W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TGL2(YDR058C)|FD-Score:5.08|P-value:1.86E-7||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TIP41(YPR040W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TOM1(YDR457W)|FD-Score:-3.75|P-value:8.86E-5||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TRM82(YDR165W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:VMA7(YGR020C)|FD-Score:4|P-value:3.22E-5||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS1(YKR001C)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS74(YDR372C)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:XKS1(YGR194C)|FD-Score:-4.89|P-value:5.09E-7||SGD DESC:Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains Gene:YBL012C(YBL012C_d)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR277C(YBR277C_d)|FD-Score:6.73|P-value:8.33E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YEF1(YEL041W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YHB1(YGR234W)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YHR175W-A(YHR175W-A_p)|FD-Score:-4.06|P-value:2.47E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIL001W(YIL001W_p)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YJL152W(YJL152W_d)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR312C(YLR312C_p)|FD-Score:3.74|P-value:9.38E-5||SGD DESC:Putative protein of unknown function Gene:YLR460C(YLR460C_p)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YMR316C-A(YMR316C-A_p)|FD-Score:5.16|P-value:1.22E-7||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YNK1(YKL067W)|FD-Score:-3.72|P-value:9.83E-5||SGD DESC:Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress Gene:YOR139C(YOR139C_d)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPL152W-A(YPL152W-A_p)|FD-Score:-3.1|P-value:9.73E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPL191C(YPL191C_p)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPT6(YLR262C)|FD-Score:4.27|P-value:9.70E-6||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ADE8(YDR408C)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ARG82(YDR173C)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ART5(YGR068C)|FD-Score:-3.78|P-value:7.74E-5||SGD DESC:Protein proposed to regulate the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane Gene:BIO4(YNR057C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BUD27(YFL023W)|FD-Score:4.99|P-value:2.98E-7||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CCR4(YAL021C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CCZ1(YBR131W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway Gene:COX7(YMR256C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CPR5(YDR304C)|FD-Score:3.77|P-value:8.02E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the endoplasmic reticulum, catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER Gene:DNM1(YLL001W)|FD-Score:-3.47|P-value:2.65E-4||SGD DESC:Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance Gene:ECM5(YMR176W)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:EMC2(YJR088C)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 Gene:EMC6(YLL014W)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:ENT4(YLL038C)|FD-Score:-4.02|P-value:2.97E-5||SGD DESC:Protein of unknown function, contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome Gene:ERG2(YMR202W)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERS1(YCR075C)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains Gene:FLC1(YPL221W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance Gene:GET2(YER083C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:HIT1(YJR055W)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HSM3(YBR272C)|FD-Score:5.24|P-value:8.05E-8||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:HSP82(YPL240C)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:IDS2(YJL146W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation Gene:IMP2'(YIL154C)|FD-Score:4.35|P-value:6.80E-6||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:MEP1(YGR121C)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MNE1(YOR350C)|FD-Score:-3.24|P-value:5.97E-4||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MTF2(YDL044C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription Gene:MYO4(YAL029C)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:NRM1(YNR009W)|FD-Score:5.33|P-value:5.04E-8||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:OSW5(YMR148W)|FD-Score:5.27|P-value:6.72E-8||SGD DESC:Protein of unknown function that may play a role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion Gene:PAL1(YDR348C)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:PES4(YFR023W)|FD-Score:-3.14|P-value:8.54E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PRY2(YKR013W)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:PTP3(YER075C)|FD-Score:-5.16|P-value:1.27E-7||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Gene:PUS7(YOR243C)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RIP1(YEL024W)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:ROT2(YBR229C)|FD-Score:-4.03|P-value:2.81E-5||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:ROX1(YPR065W)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RPE1(YJL121C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL18B(YNL301C)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPL27A(YHR010W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RRD2(YPL152W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress Gene:RTC1(YOL138C)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:RTS3(YGR161C_p)|FD-Score:4.61|P-value:2.05E-6||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:RTT106(YNL206C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Histone chaperone, involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition Gene:SKT5(YBL061C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SNF6(YHL025W)|FD-Score:-5.27|P-value:6.95E-8||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions Gene:SPO20(YMR017W)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog Gene:SRB2(YHR041C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SSH1(YBR283C)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:SVF1(YDR346C)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:TED1(YIL039W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TGL2(YDR058C)|FD-Score:5.08|P-value:1.86E-7||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TIP41(YPR040W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TOM1(YDR457W)|FD-Score:-3.75|P-value:8.86E-5||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TRM82(YDR165W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:VMA7(YGR020C)|FD-Score:4|P-value:3.22E-5||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS1(YKR001C)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS74(YDR372C)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:XKS1(YGR194C)|FD-Score:-4.89|P-value:5.09E-7||SGD DESC:Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains Gene:YBL012C(YBL012C_d)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR277C(YBR277C_d)|FD-Score:6.73|P-value:8.33E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YEF1(YEL041W)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YHB1(YGR234W)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YHR175W-A(YHR175W-A_p)|FD-Score:-4.06|P-value:2.47E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIL001W(YIL001W_p)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm Gene:YJL152W(YJL152W_d)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR312C(YLR312C_p)|FD-Score:3.74|P-value:9.38E-5||SGD DESC:Putative protein of unknown function Gene:YLR460C(YLR460C_p)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YMR316C-A(YMR316C-A_p)|FD-Score:5.16|P-value:1.22E-7||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YNK1(YKL067W)|FD-Score:-3.72|P-value:9.83E-5||SGD DESC:Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress Gene:YOR139C(YOR139C_d)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPL152W-A(YPL152W-A_p)|FD-Score:-3.1|P-value:9.73E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPL191C(YPL191C_p)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPT6(YLR262C)|FD-Score:4.27|P-value:9.70E-6||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOL144W7.425.80E-141.07NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YGR046W6.351.06E-101.02TAM41Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition
YBL105C5.334.96E-80.58PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YNL110C4.751.04E-60.29NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YPR034W4.464.14E-60.46ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YCR052W4.003.22E-50.16RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YDR047W3.846.15E-50.12HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YPR133C3.729.81E-50.08SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YLR153C3.651.34E-40.00ACS2Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
YLR009W3.641.35E-40.04RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YPL238C_d3.611.56E-40.30YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YOR210W3.304.83E-40.04RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YLR075W3.265.54E-40.06RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YNR016C3.206.77E-40.01ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YBL097W3.197.08E-40.07BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR277C_d6.738.33E-12YBR277C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W
YNR009W5.335.04E-8NRM1Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase
YMR148W5.276.72E-8OSW5Protein of unknown function that may play a role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion
YBR272C5.248.05E-8HSM3Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation
YMR316C-A_p5.161.22E-7YMR316C-A_pProtein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W
YDR058C5.081.86E-7TGL2Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli
YFL023W4.992.98E-7BUD27Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP
YPL240C4.751.03E-6HSP82Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication
YGR161C_p4.612.05E-6RTS3_pPutative component of the protein phosphatase type 2A complex
YIL154C4.356.80E-6IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YLR262C4.279.70E-6YPT6Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6
YEL024W4.221.23E-5RIP1Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly
YKR001C4.211.30E-5VPS1Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis
YBL012C_d4.151.66E-5YBL012C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR017W4.082.22E-5SPO20Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog

GO enrichment analysis for SGTC_921
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1071.74E-16SGTC_7950685-0265 78.0 μMChemDiv (Drug-like library)3027540.19403excess fatty acid
0.1054.41E-16SGTC_7301436-0052 41.2 μMChemDiv (Drug-like library)7687640.12mitochondrial processes
0.1022.98E-15SGTC_400oxethazaine 37.5 μMMiscellaneous46210.0921053calcium & mitochondrial duress
0.0961.21E-13SGTC_9242046-0044 21.3 μMChemDiv (Drug-like library)36235890.0684932
0.0936.35E-13SGTC_11780988-0033 191.0 μMChemDiv (Drug-like library)53922070.1125
0.0921.63E-12SGTC_690096-0274 206.7 μMChemDiv (Drug-like library)67413920.0512821mitochondrial processes
0.0897.84E-12SGTC_23369001819 198.7 μMChembridge (Fragment library)29896020.134328heme biosynthesis & mitochondrial translocase
0.0855.96E-11SGTC_2523spermine 47.7 μMMicrosource (Natural product library)11030.0344828calcium & mitochondrial duress
0.0857.11E-11SGTC_10573448-5381 134.0 μMChemDiv (Drug-like library)54979110.0512821calcium & mitochondrial duress
0.0841.31E-10SGTC_5203584-0023 682.0 μMChemDiv (Drug-like library)13407900.0897436
0.0814.75E-10SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.03125ERG2
0.0806.48E-10SGTC_5623772-2733 112.0 μMChemDiv (Drug-like library)28768740.0963855ERG2
0.0806.65E-10SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.112676ERG2
0.0782.24E-9SGTC_273hydroxyurea 18.1 mMMiscellaneous36570.0740741heme biosynthesis & mitochondrial translocase
0.0773.73E-9SGTC_2617dihydrotanshinone i 20.8 μMMicrosource (Natural product library)53167430.0617284copper-dependent oxidative stress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_10004048-08848.67 μM0.2608713040088ChemDiv (Drug-like library)287.249261.60508copper-dependent oxidative stress
SGTC_14944358-1428177 μM0.259741564571ChemDiv (Drug-like library)336.813243.11115
SGTC_7600568-072582.9 μM0.2575763916171ChemDiv (Drug-like library)380.010345.16613
SGTC_2890904672958.44 μM0.253731903329Chembridge (Drug-like library)270.114662.88412Golgi
SGTC_8991083-0020478 μM0.25767123ChemDiv (Drug-like library)306.701143.15714
SGTC_295690761962.52 μM0.2516423868Chembridge (Drug-like library)331.194442.54314
SGTC_7251497-096319 μM0.2424243549977ChemDiv (Drug-like library)356.117783.73215copper-dependent oxidative stress
SGTC_13191306-015543.7 μM0.2424243122360ChemDiv (Drug-like library)252.657063.06724
SGTC_12001643-019318.4 μM0.23755403974ChemDiv (Drug-like library)347.753063.56315
SGTC_750159-0059199.01 μM0.2352945377270ChemDiv (Drug-like library)251.238621.99916fatty acid desaturase (OLE1)