2046-0044

10-ethyl-N-(4-methylphenyl)acridin-9-imine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_924
Screen concentration 21.3 μM
Source ChemDiv (Drug-like library)
PubChem CID 3623589
SMILES CCN1C2=CC=CC=C2C(=NC3=CC=C(C=C3)C)C4=CC=CC=C41
Standardized SMILES CCN1c2ccccc2C(=Nc3ccc(C)cc3)c4ccccc14
Molecular weight 312.4076
ALogP 5.83
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.83
% growth inhibition (Hom. pool) 4.67


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3623589
Download HIP data (tab-delimited text)  (excel)
Gene:ACS2(YLR153C)|FD-Score:5.03|P-value:2.40E-7|Clearance:0.35||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:ARP4(YJL081C)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:DCP1(YOL149W)|FD-Score:3.76|P-value:8.47E-5|Clearance:0.28||SGD DESC:Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress Gene:DML1(YMR211W)|FD-Score:4.5|P-value:3.36E-6|Clearance:0.25||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:FAD1(YDL045C)|FD-Score:3.1|P-value:9.52E-4|Clearance:0.05||SGD DESC:Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin Gene:GFA1(YKL104C)|FD-Score:4.25|P-value:1.05E-5|Clearance:0.02||SGD DESC:Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication Gene:LSG1(YGL099W)|FD-Score:5.93|P-value:1.49E-9|Clearance:0.9||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NOP8(YOL144W)|FD-Score:3.44|P-value:2.94E-4|Clearance:0.1||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PAM16(YJL104W)|FD-Score:-4.79|P-value:8.25E-7|Clearance:0||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain Gene:PDI1(YCL043C)|FD-Score:-3.76|P-value:8.65E-5|Clearance:0||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PKC1(YBL105C)|FD-Score:4.68|P-value:1.42E-6|Clearance:0.18||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRE4(YFR050C)|FD-Score:-3.12|P-value:9.01E-4|Clearance:0||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PWP2(YCR057C)|FD-Score:-3.86|P-value:5.62E-5|Clearance:0||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RPL28(YGL103W)|FD-Score:3.44|P-value:2.89E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance Gene:RSC6(YCR052W)|FD-Score:3.21|P-value:6.66E-4|Clearance:0.1||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SMC4(YLR086W)|FD-Score:4.24|P-value:1.14E-5|Clearance:0.21||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SOF1(YLL011W)|FD-Score:-3.95|P-value:3.85E-5|Clearance:0||SGD DESC:Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p Gene:TRS120(YDR407C)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.11||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UFE1(YOR075W)|FD-Score:4.03|P-value:2.81E-5|Clearance:0.25||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:WBP1(YEL002C)|FD-Score:3.48|P-value:2.50E-4|Clearance:0.04||SGD DESC:Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum Gene:YBL073W(YBL073W_d)|FD-Score:3.23|P-value:6.08E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C Gene:YPL238C(YPL238C_d)|FD-Score:-3.68|P-value:1.16E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YTH1(YPR107C)|FD-Score:3.78|P-value:7.89E-5|Clearance:0.02||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ACS2(YLR153C)|FD-Score:5.03|P-value:2.40E-7|Clearance:0.35||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:ARP4(YJL081C)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:DCP1(YOL149W)|FD-Score:3.76|P-value:8.47E-5|Clearance:0.28||SGD DESC:Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress Gene:DML1(YMR211W)|FD-Score:4.5|P-value:3.36E-6|Clearance:0.25||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:FAD1(YDL045C)|FD-Score:3.1|P-value:9.52E-4|Clearance:0.05||SGD DESC:Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin Gene:GFA1(YKL104C)|FD-Score:4.25|P-value:1.05E-5|Clearance:0.02||SGD DESC:Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication Gene:LSG1(YGL099W)|FD-Score:5.93|P-value:1.49E-9|Clearance:0.9||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NOP8(YOL144W)|FD-Score:3.44|P-value:2.94E-4|Clearance:0.1||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PAM16(YJL104W)|FD-Score:-4.79|P-value:8.25E-7|Clearance:0||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain Gene:PDI1(YCL043C)|FD-Score:-3.76|P-value:8.65E-5|Clearance:0||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PKC1(YBL105C)|FD-Score:4.68|P-value:1.42E-6|Clearance:0.18||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRE4(YFR050C)|FD-Score:-3.12|P-value:9.01E-4|Clearance:0||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PWP2(YCR057C)|FD-Score:-3.86|P-value:5.62E-5|Clearance:0||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RPL28(YGL103W)|FD-Score:3.44|P-value:2.89E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance Gene:RSC6(YCR052W)|FD-Score:3.21|P-value:6.66E-4|Clearance:0.1||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SMC4(YLR086W)|FD-Score:4.24|P-value:1.14E-5|Clearance:0.21||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SOF1(YLL011W)|FD-Score:-3.95|P-value:3.85E-5|Clearance:0||SGD DESC:Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p Gene:TRS120(YDR407C)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.11||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UFE1(YOR075W)|FD-Score:4.03|P-value:2.81E-5|Clearance:0.25||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:WBP1(YEL002C)|FD-Score:3.48|P-value:2.50E-4|Clearance:0.04||SGD DESC:Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum Gene:YBL073W(YBL073W_d)|FD-Score:3.23|P-value:6.08E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C Gene:YPL238C(YPL238C_d)|FD-Score:-3.68|P-value:1.16E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YTH1(YPR107C)|FD-Score:3.78|P-value:7.89E-5|Clearance:0.02||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3623589
Download HOP data (tab-delimited text)  (excel)
Gene:ARF3(YOR094W)|FD-Score:-3.89|P-value:5.01E-5||SGD DESC:Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity; also has mRNA binding activity Gene:ATC1(YDR184C)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:ATG11(YPR049C)|FD-Score:3.99|P-value:3.33E-5||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:ATG31(YDR022C)|FD-Score:-3.11|P-value:9.21E-4||SGD DESC:Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion Gene:AUA1(YFL010W-A)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:CHO1(YER026C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CHZ1(YER030W)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress Gene:COG8(YML071C)|FD-Score:-3.88|P-value:5.32E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ10(YOL008W)|FD-Score:-4.9|P-value:4.84E-7||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:COX6(YHR051W)|FD-Score:4.56|P-value:2.58E-6||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:DIA2(YOR080W)|FD-Score:-5.37|P-value:3.83E-8||SGD DESC:Origin-binding F-box protein; forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication; plays a role in transcription; required for correct assembly of RSC complex, correct RSC-mediated transcription regulation, and correct nucleosome positioning; involved in invasive and pseudohyphal growth; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:DLD2(YDL178W)|FD-Score:-3.81|P-value:6.89E-5||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:EMC6(YLL014W)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:ERV2(YPR037C)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER Gene:EXG2(YDR261C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FUN14(YAL008W)|FD-Score:3.89|P-value:4.97E-5||SGD DESC:Mitochondrial protein of unknown function Gene:GEX2(YKR106W)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GPR1(YDL035C)|FD-Score:-3.93|P-value:4.25E-5||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:GUT2(YIL155C)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner Gene:GYL1(YMR192W)|FD-Score:-4.51|P-value:3.30E-6||SGD DESC:Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HOM6(YJR139C)|FD-Score:14.5|P-value:4.63E-48||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HOP2(YGL033W)|FD-Score:-3.11|P-value:9.31E-4||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HSM3(YBR272C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:LSC1(YOR142W)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Gene:LTV1(YKL143W)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MET3(YJR010W)|FD-Score:-3.8|P-value:7.38E-5||SGD DESC:ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism Gene:MKT1(YNL085W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MNE1(YOR350C)|FD-Score:-3.99|P-value:3.29E-5||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MNN10(YDR245W)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MPD1(YOR288C)|FD-Score:5.68|P-value:6.70E-9||SGD DESC:Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation Gene:MSN2(YMR037C)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:MTM1(YGR257C)|FD-Score:-4.72|P-value:1.19E-6||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:PEX22(YAL055W)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:PIN4(YBL051C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PPM2(YOL141W)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p Gene:PUS1(YPL212C)|FD-Score:-4.05|P-value:2.59E-5||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:REH1(YLR387C)|FD-Score:3.79|P-value:7.40E-5||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RFM1(YOR279C)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RIM9(YMR063W)|FD-Score:3.76|P-value:8.66E-5||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RKM3(YBR030W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein Gene:RLF2(YPR018W)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RMD1(YDL001W)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Cytoplasmic protein required for sporulation Gene:RPL16A(YIL133C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL18B(YNL301C)|FD-Score:-4.89|P-value:4.99E-7||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:4.67|P-value:1.53E-6||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPP2A(YOL039W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:SCD6(YPR129W)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SEC72(YLR292C)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SHR5(YOL110W)|FD-Score:4.35|P-value:6.74E-6||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SKS1(YPL026C)|FD-Score:-4.5|P-value:3.45E-6||SGD DESC:Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway Gene:SLM6(YBR266C_d)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SNF11(YDR073W)|FD-Score:-5.64|P-value:8.50E-9||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions Gene:SRB5(YGR104C)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SUM1(YDR310C)|FD-Score:5.56|P-value:1.39E-8||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SUR4(YLR372W)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TDA1(YMR291W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:THO2(YNL139C)|FD-Score:6.15|P-value:3.83E-10||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TRK1(YJL129C)|FD-Score:4.34|P-value:7.04E-6||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRP4(YDR354W)|FD-Score:-5.48|P-value:2.11E-8||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UNG1(YML021C)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:YDL050C(YDL050C_d)|FD-Score:-6.2|P-value:2.81E-10||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR161W(YDR161W_p)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YDR182W-A(YDR182W-A_p)|FD-Score:4.82|P-value:7.07E-7||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YER091C-A(YER091C-A_d)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR149W(YGR149W_p)|FD-Score:-3.75|P-value:8.71E-5||SGD DESC:Putative protein of unknown function; predicted to be an integal membrane protein Gene:YGR259C(YGR259C_d)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YGR291C(YGR291C_d)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR112C(YHR112C)|FD-Score:-3.1|P-value:9.54E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway Gene:YNL226W(YNL226W_d)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YNL266W(YNL266W_d)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C Gene:YOR345C(YOR345C_d)|FD-Score:-3.77|P-value:8.20E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YPL152W-A(YPL152W-A_p)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPR050C(YPR050C_d)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPS5(YGL259W_p)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Protein with similarity to GPI-anchored aspartic proteases such as Yap1p and Yap3p Gene:ARF3(YOR094W)|FD-Score:-3.89|P-value:5.01E-5||SGD DESC:Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity; also has mRNA binding activity Gene:ATC1(YDR184C)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:ATG11(YPR049C)|FD-Score:3.99|P-value:3.33E-5||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:ATG31(YDR022C)|FD-Score:-3.11|P-value:9.21E-4||SGD DESC:Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion Gene:AUA1(YFL010W-A)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:CHO1(YER026C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CHZ1(YER030W)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress Gene:COG8(YML071C)|FD-Score:-3.88|P-value:5.32E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ10(YOL008W)|FD-Score:-4.9|P-value:4.84E-7||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:COX6(YHR051W)|FD-Score:4.56|P-value:2.58E-6||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:DIA2(YOR080W)|FD-Score:-5.37|P-value:3.83E-8||SGD DESC:Origin-binding F-box protein; forms an SCF ubiquitin ligase complex with Skp1p and Cdc53p; plays a role in DNA replication; plays a role in transcription; required for correct assembly of RSC complex, correct RSC-mediated transcription regulation, and correct nucleosome positioning; involved in invasive and pseudohyphal growth; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:DLD2(YDL178W)|FD-Score:-3.81|P-value:6.89E-5||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:EMC6(YLL014W)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:ERV2(YPR037C)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER Gene:EXG2(YDR261C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FUN14(YAL008W)|FD-Score:3.89|P-value:4.97E-5||SGD DESC:Mitochondrial protein of unknown function Gene:GEX2(YKR106W)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GPR1(YDL035C)|FD-Score:-3.93|P-value:4.25E-5||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:GUT2(YIL155C)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner Gene:GYL1(YMR192W)|FD-Score:-4.51|P-value:3.30E-6||SGD DESC:Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HOM6(YJR139C)|FD-Score:14.5|P-value:4.63E-48||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HOP2(YGL033W)|FD-Score:-3.11|P-value:9.31E-4||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HSM3(YBR272C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:LSC1(YOR142W)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Alpha subunit of succinyl-CoA ligase, which is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated Gene:LTV1(YKL143W)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MET3(YJR010W)|FD-Score:-3.8|P-value:7.38E-5||SGD DESC:ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism Gene:MKT1(YNL085W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MNE1(YOR350C)|FD-Score:-3.99|P-value:3.29E-5||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MNN10(YDR245W)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MPD1(YOR288C)|FD-Score:5.68|P-value:6.70E-9||SGD DESC:Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation Gene:MSN2(YMR037C)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:MTM1(YGR257C)|FD-Score:-4.72|P-value:1.19E-6||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:PEX22(YAL055W)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:PIN4(YBL051C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PPM2(YOL141W)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p Gene:PUS1(YPL212C)|FD-Score:-4.05|P-value:2.59E-5||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:REH1(YLR387C)|FD-Score:3.79|P-value:7.40E-5||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RFM1(YOR279C)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RIM9(YMR063W)|FD-Score:3.76|P-value:8.66E-5||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RKM3(YBR030W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein Gene:RLF2(YPR018W)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RMD1(YDL001W)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Cytoplasmic protein required for sporulation Gene:RPL16A(YIL133C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL18B(YNL301C)|FD-Score:-4.89|P-value:4.99E-7||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:4.67|P-value:1.53E-6||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPP2A(YOL039W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:SCD6(YPR129W)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SEC72(YLR292C)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SHR5(YOL110W)|FD-Score:4.35|P-value:6.74E-6||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SKS1(YPL026C)|FD-Score:-4.5|P-value:3.45E-6||SGD DESC:Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway Gene:SLM6(YBR266C_d)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SNF11(YDR073W)|FD-Score:-5.64|P-value:8.50E-9||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions Gene:SRB5(YGR104C)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SUM1(YDR310C)|FD-Score:5.56|P-value:1.39E-8||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SUR4(YLR372W)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TDA1(YMR291W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:THO2(YNL139C)|FD-Score:6.15|P-value:3.83E-10||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TRK1(YJL129C)|FD-Score:4.34|P-value:7.04E-6||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRP4(YDR354W)|FD-Score:-5.48|P-value:2.11E-8||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UNG1(YML021C)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:YDL050C(YDL050C_d)|FD-Score:-6.2|P-value:2.81E-10||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR161W(YDR161W_p)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YDR182W-A(YDR182W-A_p)|FD-Score:4.82|P-value:7.07E-7||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YER091C-A(YER091C-A_d)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR149W(YGR149W_p)|FD-Score:-3.75|P-value:8.71E-5||SGD DESC:Putative protein of unknown function; predicted to be an integal membrane protein Gene:YGR259C(YGR259C_d)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YGR291C(YGR291C_d)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHR112C(YHR112C)|FD-Score:-3.1|P-value:9.54E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway Gene:YNL226W(YNL226W_d)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YNL266W(YNL266W_d)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C Gene:YOR345C(YOR345C_d)|FD-Score:-3.77|P-value:8.20E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YPL152W-A(YPL152W-A_p)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPR050C(YPR050C_d)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPS5(YGL259W_p)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Protein with similarity to GPI-anchored aspartic proteases such as Yap1p and Yap3p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W5.931.49E-90.90LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YLR153C5.032.40E-70.35ACS2Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
YBL105C4.681.42E-60.18PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YMR211W4.503.36E-60.25DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YKL104C4.251.05E-50.02GFA1Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication
YLR086W4.241.14E-50.21SMC4Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate
YOR075W4.032.81E-50.25UFE1t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER
YPR107C3.787.89E-50.02YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YOL149W3.768.47E-50.28DCP1Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress
YEL002C3.482.50E-40.04WBP1Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum
YGL103W3.442.89E-40.00RPL28Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance
YOL144W3.442.94E-40.10NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YDR407C3.344.17E-40.11TRS120One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YBL073W_d3.236.08E-40.03YBL073W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C
YCR052W3.216.66E-40.10RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJR139C14.504.63E-48HOM6Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
YNL139C6.153.83E-10THO2Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids
YOR288C5.686.70E-9MPD1Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation
YDR310C5.561.39E-8SUM1Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint
YDR182W-A_p4.827.07E-7YDR182W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YLR061W4.671.53E-6RPL22ARibosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication
YML021C4.631.85E-6UNG1Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus
YHR051W4.562.58E-6COX6Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels
YOL110W4.356.74E-6SHR5Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization
YJL129C4.347.04E-6TRK1Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
YLR292C4.181.46E-5SEC72Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YGR104C4.022.88E-5SRB5Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance
YBR266C_d4.013.08E-5SLM6_dProtein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase
YLL014W3.993.25E-5EMC6Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93
YPR049C3.993.33E-5ATG11Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS)

GO enrichment analysis for SGTC_924
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2722.19E-100SGTC_492tmb-8 126.0 μMICCB bioactive library54940.078125
0.1337.78E-25SGTC_33479188721 44.7 μMChembridge (Drug-like library)441184110.107143
0.1301.22E-23SGTC_1954234-0266 16.7 μMChemDiv (Drug-like library)23033540.122807
0.1223.51E-21SGTC_3630455-0021 3.1 μMChemDiv (Drug-like library)45974660.0967742azole & statin
0.1101.92E-17SGTC_120rhodamine 6G 52.3 μMChemDiv (Drug-like library)652110.135802mitochondrial processes
0.1093.74E-17SGTC_1696st025591 34.8 μMTimTec (Natural product derivative library)27871470.0923077SWF1 & branched chain AA biosynthesis
0.1094.34E-17SGTC_1786berberine 21.6 μMMiscellaneous124560.069444460S ribosome export
0.1055.81E-16SGTC_451chloral hydrate 100.0 μMMiscellaneous2707NA
0.1041.21E-15SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.14102660S ribosome export
0.1041.34E-15SGTC_8660495-0101 262.0 μMChemDiv (Drug-like library)6356940.05633860S ribosome export
0.1015.86E-15SGTC_2689tacrolimus 100.0 μMNIH Clinical Collection235817960.0517241
0.1019.80E-15SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.13235360S ribosome export
0.1001.68E-14SGTC_12490481-0015 10.2 μMChemDiv (Drug-like library)42275770.185185
0.0984.60E-14SGTC_260nsc-65238 4.0 μMMiscellaneous32462520.0735294endomembrane recycling
0.0979.83E-14SGTC_9312812-0076 566.0 μMChemDiv (Drug-like library)34509590.25

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_13161272-005065.9 μM0.3404267340392ChemDiv (Drug-like library)251.283222.02213
SGTC_6660272-0154136 μM0.3272736870312ChemDiv (Drug-like library)276.357342.41523cell wall
SGTC_20225136654200 μM0.3111111713771Chembridge (Fragment library)204.225180.99703
SGTC_12330325-040814.2 μM0.28205112874ChemDiv (Drug-like library)211.262423.93813
SGTC_20024023503131 μM0.2765964736863Chembridge (Fragment library)193.1744231.4303mitochondrial processes
SGTC_23369001819198.65 μM0.2765962989602Chembridge (Fragment library)209.629021.88902heme biosynthesis & mitochondrial translocase
SGTC_1915st06710530.6 μM0.27569751TimTec (Natural product derivative library)209.243243.1602RNA pol III & RNase P/MRP
SGTC_1686st019653115.5 μM0.27272797252TimTec (Natural product derivative library)173.211141.90401mitochondrial processes
SGTC_544k015-002052.4 μM0.2653063008304ChemDiv (Drug-like library)316.14943.20802
SGTC_606k015-000838.6 μM0.2653061970475ChemDiv (Drug-like library)306.143463.78802