2805-0021

N-(4-methoxy-2-nitrophenyl)-2-quinolin-8-ylsulfanylacetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_929
Screen concentration 398.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 3098024
SMILES COC1=CC(=C(C=C1)NC(=O)CSC2=CC=CC3=C2N=CC=C3)[N+](=O)[O-]
Standardized SMILES COc1ccc(NC(=O)CSc2cccc3cccnc23)c(c1)[N+](=O)[O-]
Molecular weight 369.3944
ALogP 3.07
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.62
% growth inhibition (Hom. pool) 3.33


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3098024
Download HIP data (tab-delimited text)  (excel)
Gene:CDC25(YLR310C)|FD-Score:3.73|P-value:9.47E-5|Clearance:0.22||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CFT1(YDR301W)|FD-Score:3.38|P-value:3.60E-4|Clearance:0.11||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:GPI11(YDR302W)|FD-Score:3.82|P-value:6.62E-5|Clearance:0.09||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:JIP5(YPR169W)|FD-Score:3.5|P-value:2.34E-4|Clearance:0.03||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:KAR1(YNL188W)|FD-Score:3.27|P-value:5.35E-4|Clearance:0.18||SGD DESC:Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p Gene:POL2(YNL262W)|FD-Score:4.69|P-value:1.35E-6|Clearance:0.87||SGD DESC:Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p Gene:PRE3(YJL001W)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.05||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:RPP0(YLR340W)|FD-Score:3.47|P-value:2.65E-4|Clearance:0.03||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RRP1(YDR087C)|FD-Score:6.22|P-value:2.54E-10|Clearance:1.52||SGD DESC:Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles Gene:TID3(YIL144W)|FD-Score:-3.75|P-value:8.72E-5|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:YGL074C(YGL074C_d)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Gene:YLR198C(YLR198C_d)|FD-Score:3.1|P-value:9.80E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:CDC25(YLR310C)|FD-Score:3.73|P-value:9.47E-5|Clearance:0.22||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CFT1(YDR301W)|FD-Score:3.38|P-value:3.60E-4|Clearance:0.11||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:GPI11(YDR302W)|FD-Score:3.82|P-value:6.62E-5|Clearance:0.09||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:JIP5(YPR169W)|FD-Score:3.5|P-value:2.34E-4|Clearance:0.03||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:KAR1(YNL188W)|FD-Score:3.27|P-value:5.35E-4|Clearance:0.18||SGD DESC:Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p Gene:POL2(YNL262W)|FD-Score:4.69|P-value:1.35E-6|Clearance:0.87||SGD DESC:Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p Gene:PRE3(YJL001W)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.05||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:RPP0(YLR340W)|FD-Score:3.47|P-value:2.65E-4|Clearance:0.03||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RRP1(YDR087C)|FD-Score:6.22|P-value:2.54E-10|Clearance:1.52||SGD DESC:Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles Gene:TID3(YIL144W)|FD-Score:-3.75|P-value:8.72E-5|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:YGL074C(YGL074C_d)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor Gene:YLR198C(YLR198C_d)|FD-Score:3.1|P-value:9.80E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3098024
Download HOP data (tab-delimited text)  (excel)
Gene:ADH6(YMR318C)|FD-Score:4|P-value:3.13E-5||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:ARA1(YBR149W)|FD-Score:-4.52|P-value:3.11E-6||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ATG29(YPL166W)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BEM1(YBR200W)|FD-Score:-3.52|P-value:2.20E-4||SGD DESC:Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p Gene:CLA4(YNL298W)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLB1(YGR108W)|FD-Score:3.74|P-value:9.37E-5||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COA2(YPL189C-A)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:DEP1(YAL013W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:DET1(YDR051C)|FD-Score:4.82|P-value:7.22E-7||SGD DESC:Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel Gene:DIG1(YPL049C)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DUR3(YHL016C)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:ECM7(YLR443W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:ENT5(YDR153C)|FD-Score:4.48|P-value:3.77E-6||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin Gene:ENV11(YGR071C)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:ERP3(YDL018C)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:GCS1(YDL226C)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GLE2(YER107C)|FD-Score:-3.19|P-value:7.01E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GTT3(YEL017W)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:HOP1(YIL072W)|FD-Score:-5.25|P-value:7.78E-8||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:IRC4(YDR540C)|FD-Score:6.03|P-value:8.36E-10||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:KKQ8(YKL168C)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:KNH1(YDL049C)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:MRPL10(YNL284C)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:NEW1(YPL226W)|FD-Score:4.65|P-value:1.68E-6||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NGL1(YOL042W)|FD-Score:-5.15|P-value:1.29E-7||SGD DESC:Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NRM1(YNR009W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:PEX30(YLR324W)|FD-Score:-3.1|P-value:9.84E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PEX32(YBR168W)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHO88(YBR106W)|FD-Score:4.91|P-value:4.66E-7||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PTH1(YHR189W)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RPL19A(YBR084C-A)|FD-Score:-3.25|P-value:5.78E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL43A(YPR043W)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:RPN10(YHR200W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RTT10(YPL183C)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SAM37(YMR060C)|FD-Score:4.5|P-value:3.35E-6||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SAS3(YBL052C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SIP3(YNL257C)|FD-Score:4.55|P-value:2.64E-6||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SKG1(YKR100C)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:TPO4(YOR273C)|FD-Score:4.55|P-value:2.67E-6||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRM12(YML005W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA Gene:TRS85(YDR108W)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:UBP8(YMR223W)|FD-Score:-3.91|P-value:4.68E-5||SGD DESC:Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B Gene:URA8(YJR103W)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication Gene:VHR1(YIL056W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication Gene:VOA1(YGR106C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS61(YDR136C_d)|FD-Score:3.93|P-value:4.31E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAL067W-A(YAL067W-A_p)|FD-Score:-3.8|P-value:7.11E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR285W(YBR285W_p)|FD-Score:7.16|P-value:4.11E-13||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YCL023C(YCL023C_d)|FD-Score:3.1|P-value:9.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 Gene:YDR008C(YDR008C_d)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR089W(YDR089W_p)|FD-Score:4.4|P-value:5.36E-6||SGD DESC:Protein of unknown function; deletion confers resistance to nickel; contains an SPX domain, which is found in proteins involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:YER068C-A(YER068C-A_d)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER130C(YER130C_p)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YFR045W(YFR045W_p)|FD-Score:4.57|P-value:2.39E-6||SGD DESC:Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white Gene:YIL060W(YIL060W_p)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YIL163C(YIL163C_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YIL166C(YIL166C_p)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene Gene:YJL147C(YJL147C_p)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Mitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene Gene:YKL136W(YKL136W_d)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YLR173W(YLR173W_p)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Putative protein of unknown function Gene:YLR217W(YLR217W_d)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 Gene:YNL276C(YNL276C_d)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YPR050C(YPR050C_d)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPR064W(YPR064W_d)|FD-Score:-4.08|P-value:2.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR077C(YPR077C_d)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPR109W(YPR109W_p)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:YPR123C(YPR123C_d)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPT52(YKR014C)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:ADH6(YMR318C)|FD-Score:4|P-value:3.13E-5||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:ARA1(YBR149W)|FD-Score:-4.52|P-value:3.11E-6||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ATG29(YPL166W)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BEM1(YBR200W)|FD-Score:-3.52|P-value:2.20E-4||SGD DESC:Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p Gene:CLA4(YNL298W)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CLB1(YGR108W)|FD-Score:3.74|P-value:9.37E-5||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:COA2(YPL189C-A)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:DEP1(YAL013W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:DET1(YDR051C)|FD-Score:4.82|P-value:7.22E-7||SGD DESC:Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel Gene:DIG1(YPL049C)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DUR3(YHL016C)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:ECM7(YLR443W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:ENT5(YDR153C)|FD-Score:4.48|P-value:3.77E-6||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin Gene:ENV11(YGR071C)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:ERP3(YDL018C)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:GCS1(YDL226C)|FD-Score:3.14|P-value:8.50E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GLE2(YER107C)|FD-Score:-3.19|P-value:7.01E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GTT3(YEL017W)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:HOP1(YIL072W)|FD-Score:-5.25|P-value:7.78E-8||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:IRC4(YDR540C)|FD-Score:6.03|P-value:8.36E-10||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:KKQ8(YKL168C)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:KNH1(YDL049C)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:MRPL10(YNL284C)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:NEW1(YPL226W)|FD-Score:4.65|P-value:1.68E-6||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:NGL1(YOL042W)|FD-Score:-5.15|P-value:1.29E-7||SGD DESC:Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NRM1(YNR009W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase Gene:PEX30(YLR324W)|FD-Score:-3.1|P-value:9.84E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PEX32(YBR168W)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHO88(YBR106W)|FD-Score:4.91|P-value:4.66E-7||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PTH1(YHR189W)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RPL19A(YBR084C-A)|FD-Score:-3.25|P-value:5.78E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL43A(YPR043W)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:RPN10(YHR200W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RTT10(YPL183C)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SAM37(YMR060C)|FD-Score:4.5|P-value:3.35E-6||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SAS3(YBL052C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal Gene:SIP3(YNL257C)|FD-Score:4.55|P-value:2.64E-6||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SKG1(YKR100C)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:TPO4(YOR273C)|FD-Score:4.55|P-value:2.67E-6||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRM12(YML005W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; required for wybutosine formation in phenylalanine-accepting tRNA Gene:TRS85(YDR108W)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:UBP8(YMR223W)|FD-Score:-3.91|P-value:4.68E-5||SGD DESC:Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B Gene:URA8(YJR103W)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication Gene:VHR1(YIL056W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication Gene:VOA1(YGR106C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS61(YDR136C_d)|FD-Score:3.93|P-value:4.31E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAL067W-A(YAL067W-A_p)|FD-Score:-3.8|P-value:7.11E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR285W(YBR285W_p)|FD-Score:7.16|P-value:4.11E-13||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YCL023C(YCL023C_d)|FD-Score:3.1|P-value:9.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4 Gene:YDR008C(YDR008C_d)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR089W(YDR089W_p)|FD-Score:4.4|P-value:5.36E-6||SGD DESC:Protein of unknown function; deletion confers resistance to nickel; contains an SPX domain, which is found in proteins involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:YER068C-A(YER068C-A_d)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER130C(YER130C_p)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YFR045W(YFR045W_p)|FD-Score:4.57|P-value:2.39E-6||SGD DESC:Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white Gene:YIL060W(YIL060W_p)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YIL163C(YIL163C_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YIL166C(YIL166C_p)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene Gene:YJL147C(YJL147C_p)|FD-Score:-3.34|P-value:4.20E-4||SGD DESC:Mitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene Gene:YKL136W(YKL136W_d)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YLR173W(YLR173W_p)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Putative protein of unknown function Gene:YLR217W(YLR217W_d)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6 Gene:YNL276C(YNL276C_d)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YPR050C(YPR050C_d)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPR064W(YPR064W_d)|FD-Score:-4.08|P-value:2.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR077C(YPR077C_d)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPR109W(YPR109W_p)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:YPR123C(YPR123C_d)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPT52(YKR014C)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR087C6.222.54E-101.52RRP1Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles
YNL262W4.691.35E-60.87POL2Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p
YDR302W3.826.62E-50.09GPI11ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
YLR310C3.739.47E-50.22CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YGL074C_d3.522.18E-40.02YGL074C_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
YPR169W3.502.34E-40.03JIP5Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein
YLR340W3.472.65E-40.04RPP0Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
YJL001W3.433.02E-40.05PRE3Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
YDR301W3.383.60E-40.11CFT1RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF
YNL188W3.275.35E-40.17KAR1Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p
YLR198C_d3.109.80E-40.05YLR198C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W
YLR208W3.050.001150.05SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YMR239C3.000.001370.01RNT1Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes
YBL041W2.980.001430.08PRE7Beta 6 subunit of the 20S proteasome
YBR070C2.900.001840.03ALG14Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR285W_p7.164.11E-13YBR285W_pPutative protein of unknown function; YBR285W is not an essential gene
YDR540C6.038.36E-10IRC4Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
YBR106W4.914.66E-7PHO88Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
YDR051C4.827.22E-7DET1Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
YKR100C4.701.31E-6SKG1Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell
YPL226W4.651.68E-6NEW1ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction
YFR045W_p4.572.39E-6YFR045W_pPutative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white
YNL257C4.552.64E-6SIP3Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication
YOR273C4.552.67E-6TPO4Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane
YMR060C4.503.35E-6SAM37Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability
YDR153C4.483.77E-6ENT5Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin
YDR089W_p4.405.36E-6YDR089W_pProtein of unknown function; deletion confers resistance to nickel; contains an SPX domain, which is found in proteins involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress
YNR009W4.151.63E-5NRM1Transcriptional co-repressor of MBF (MCB binding factor)-regulated gene expression; Nrm1p associates stably with promoters via MBF to repress transcription upon exit from G1 phase
YMR318C4.003.13E-5ADH6NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress
YDR136C_d3.934.31E-5VPS61_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect

GO enrichment analysis for SGTC_929
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0889.67E-12SGTC_2735sertaconazole 140.0 nMMiscellaneous658630.102041plasma membrane duress
0.0773.57E-9SGTC_31569099931 49.5 μMChembridge (Drug-like library)181110530.117647
0.0691.12E-7SGTC_13191306-0155 43.7 μMChemDiv (Drug-like library)31223600.155844
0.0691.22E-7SGTC_8680527-0199 213.0 μMChemDiv (Drug-like library)28494500.156627fatty acid desaturase (OLE1)
0.0595.44E-6SGTC_4341-hexadecyl-2-methylglycero-3 PC 8.0 μMICCB bioactive library13770.0681818
0.0587.40E-6SGTC_7414582-0002 126.0 μMChemDiv (Drug-like library)29126880.0632911
0.0587.45E-6SGTC_11851319-0113 81.3 μMChemDiv (Drug-like library)46727630.108911DNA damage response
0.0588.76E-6SGTC_19284'-methoxychalcone 40.3 μMTimTec (Natural product derivative library)6418180.2
0.0571.03E-5SGTC_20384100038 31.0 μMChembridge (Fragment library)35968030.0506329plasma membrane duress
0.0571.19E-5SGTC_450aminopyrene 156.0 nMMiscellaneous153520.104478
0.0571.29E-5SGTC_8340312-0008 75.7 μMChemDiv (Drug-like library)63855730.111111NEO1
0.0561.76E-5SGTC_1782st039423 80.6 μMTimTec (Natural product derivative library)20681690.125
0.0552.07E-5SGTC_8750709-0210 25.7 μMChemDiv (Drug-like library)62513640.139241fatty acid desaturase (OLE1)
0.0552.23E-5SGTC_2725haloperidol 53.2 μMMiscellaneous35590.0769231plasma membrane duress
0.0543.02E-5SGTC_1923455-0609 60.9 μMChemDiv (Drug-like library)37696390.0533333

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_6520437-0092114 μM0.3827163091274ChemDiv (Drug-like library)507.775385.60636
SGTC_8151582-002736.9 μM0.3661973128206ChemDiv (Drug-like library)286.24292.82717
SGTC_8991083-0020478 μM0.347222767123ChemDiv (Drug-like library)306.701143.15714
SGTC_2000st07525436.4 μM0.34117617234175TimTec (Natural product derivative library)549.594883.7210
SGTC_2978901178442.97 μM0.32994934Chembridge (Drug-like library)309.38223.67423
SGTC_15153460-003142.1 μM0.2962962876596ChemDiv (Drug-like library)350.410983.6224DNA intercalators
SGTC_2842900067519.48 μM0.2957752989072Chembridge (Drug-like library)258.272563.114
SGTC_10044084-0029199 μM0.291667716894ChemDiv (Drug-like library)323.412083.22414
SGTC_3109912285349.47 μM0.2916671234709Chembridge (Drug-like library)287.376683.17413
SGTC_10004048-08848.67 μM0.28767113040088ChemDiv (Drug-like library)287.249261.60508copper-dependent oxidative stress