3005-4620

N-(2-methoxyphenyl)-4-(tetrazol-1-yl)benzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_936
Screen concentration 401.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 777207
SMILES COC1=CC=CC=C1NC(=O)C2=CC=C(C=C2)N3C=NN=N3
Standardized SMILES COc1ccccc1NC(=O)c2ccc(cc2)n3cnnn3
Molecular weight 295.296
ALogP 1.49
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.53
% growth inhibition (Hom. pool) 3.76


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 777207
Download HIP data (tab-delimited text)  (excel)
Gene:LSG1(YGL099W)|FD-Score:4.13|P-value:1.81E-5|Clearance:0.68||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NOP56(YLR197W)|FD-Score:3.3|P-value:4.79E-4|Clearance:0.13||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PDS5(YMR076C)|FD-Score:4.77|P-value:9.31E-7|Clearance:0.52||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PRE4(YFR050C)|FD-Score:4.24|P-value:1.10E-5|Clearance:0.11||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRP9(YDL030W)|FD-Score:3.34|P-value:4.14E-4|Clearance:0.04||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:PUP1(YOR157C)|FD-Score:-3.99|P-value:3.24E-5|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RFA3(YJL173C)|FD-Score:6.75|P-value:7.24E-12|Clearance:1.99||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RPB8(YOR224C)|FD-Score:-6.3|P-value:1.50E-10|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:SAR1(YPL218W)|FD-Score:-3.44|P-value:2.94E-4|Clearance:0||SGD DESC:GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport Gene:WRS1(YOL097C)|FD-Score:3.18|P-value:7.42E-4|Clearance:0.18||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YKT6(YKL196C)|FD-Score:3.45|P-value:2.78E-4|Clearance:0.11||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus Gene:YTH1(YPR107C)|FD-Score:-3.17|P-value:7.62E-4|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:LSG1(YGL099W)|FD-Score:4.13|P-value:1.81E-5|Clearance:0.68||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NOP56(YLR197W)|FD-Score:3.3|P-value:4.79E-4|Clearance:0.13||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PDS5(YMR076C)|FD-Score:4.77|P-value:9.31E-7|Clearance:0.52||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PRE4(YFR050C)|FD-Score:4.24|P-value:1.10E-5|Clearance:0.11||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRP9(YDL030W)|FD-Score:3.34|P-value:4.14E-4|Clearance:0.04||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:PUP1(YOR157C)|FD-Score:-3.99|P-value:3.24E-5|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RFA3(YJL173C)|FD-Score:6.75|P-value:7.24E-12|Clearance:1.99||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RPB8(YOR224C)|FD-Score:-6.3|P-value:1.50E-10|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:SAR1(YPL218W)|FD-Score:-3.44|P-value:2.94E-4|Clearance:0||SGD DESC:GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport Gene:WRS1(YOL097C)|FD-Score:3.18|P-value:7.42E-4|Clearance:0.18||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YKT6(YKL196C)|FD-Score:3.45|P-value:2.78E-4|Clearance:0.11||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus Gene:YTH1(YPR107C)|FD-Score:-3.17|P-value:7.62E-4|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 777207
Download HOP data (tab-delimited text)  (excel)
Gene:AIM41(YOR215C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:AMD1(YML035C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools Gene:ANB1(YJR047C)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication Gene:ARG2(YJL071W)|FD-Score:5.17|P-value:1.15E-7||SGD DESC:Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p Gene:ARG8(YOL140W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine Gene:ARO2(YGL148W)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATG8(YBL078C)|FD-Score:4.95|P-value:3.67E-7||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BUD3(YCL014W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:BUD6(YLR319C)|FD-Score:-3.21|P-value:6.55E-4||SGD DESC:Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate Gene:CBT1(YKL208W)|FD-Score:5.05|P-value:2.25E-7||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:COG1(YGL223C)|FD-Score:3.85|P-value:5.81E-5||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG5(YNL051W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX16(YJL003W)|FD-Score:-4.63|P-value:1.81E-6||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:COX23(YHR116W)|FD-Score:3.74|P-value:9.26E-5||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:CYB2(YML054C)|FD-Score:-3.78|P-value:7.83E-5||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:DCR2(YLR361C)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Phosphoesterase involved in downregulation of the unfolded protein response, at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START Gene:DEF1(YKL054C)|FD-Score:-3.2|P-value:6.77E-4||SGD DESC:RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis Gene:DFG16(YOR030W)|FD-Score:-3.79|P-value:7.53E-5||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DLD2(YDL178W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:DUG3(YNL191W)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:FAB1(YFR019W)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:FUI1(YBL042C)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:FYV6(YNL133C)|FD-Score:6.78|P-value:6.05E-12||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GRE2(YOL151W)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:GYP5(YPL249C)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:GYP8(YFL027C)|FD-Score:3.9|P-value:4.86E-5||SGD DESC:GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport Gene:HIS4(YCL030C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis Gene:HMT1(YBR034C)|FD-Score:3.79|P-value:7.41E-5||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HST2(YPL015C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HUA1(YGR268C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:HYR1(YIR037W)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:ISF1(YMR081C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant Gene:LSP1(YPL004C)|FD-Score:5.13|P-value:1.44E-7||SGD DESC:Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family Gene:MAM3(YOL060C)|FD-Score:6.77|P-value:6.64E-12||SGD DESC:Protein required for normal mitochondrial morphology, has similarity to hemolysins Gene:MAP2(YBL091C)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p Gene:MGR2(YPL098C)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MLP2(YIL149C)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length Gene:MMM1(YLL006W)|FD-Score:-4.31|P-value:8.22E-6||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRM2(YGL136C)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ Gene:MRPL7(YDR237W)|FD-Score:3.75|P-value:8.83E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:NNT1(YLR285W)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination Gene:OAR1(YKL055C)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:OAZ1(YPL052W)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:PFS1(YHR185C)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PSR1(YLL010C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:RPL22B(YFL034C-A)|FD-Score:5.59|P-value:1.13E-8||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RPS19A(YOL121C)|FD-Score:-4.32|P-value:7.96E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:SDC25(YLL016W)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SHO1(YER118C)|FD-Score:10.3|P-value:3.85E-25||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:SPO21(YOL091W)|FD-Score:-4.34|P-value:7.05E-6||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SRV2(YNL138W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:STE50(YCL032W)|FD-Score:3.85|P-value:5.82E-5||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SUN4(YNL066W)|FD-Score:3.81|P-value:7.04E-5||SGD DESC:Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family Gene:SUS1(YBR111W-A)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:TAE2(YPL009C)|FD-Score:5.19|P-value:1.04E-7||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:TAT1(YBR069C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TCB3(YML072C)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TCM62(YBR044C)|FD-Score:4.28|P-value:9.27E-6||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone Gene:TDA5(YLR426W_p)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:VMA1(YDL185W)|FD-Score:3.82|P-value:6.54E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA16(YHR026W)|FD-Score:4.35|P-value:6.81E-6||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VMA7(YGR020C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS25(YJR102C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS45(YGL095C)|FD-Score:3.9|P-value:4.86E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS52(YDR484W)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:YBL059W(YBL059W_p)|FD-Score:5.41|P-value:3.11E-8||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YBL071C-B(YBL071C-B_p)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCL074W(YCL074W)|FD-Score:-4.19|P-value:1.40E-5||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YER130C(YER130C_p)|FD-Score:3.8|P-value:7.38E-5||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YFL013W-A(YFL013W-A_d)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YFL040W(YFL040W_p)|FD-Score:3.73|P-value:9.57E-5||SGD DESC:Putative transporter, member of the sugar porter family; YFL040W is not an essential gene Gene:YGL217C(YGL217C_d)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W Gene:YGR169C-A(YGR169C-A_p)|FD-Score:4.95|P-value:3.69E-7||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YHL044W(YHL044W_p)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YIL134C-A(YIL134C-A_p)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIL152W(YIL152W_p)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:Putative protein of unknown function Gene:YJL027C(YJL027C_p)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Putative protein of unknown function Gene:YJL144W(YJL144W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Cytoplasmic hydrophilin essential in dessication-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress Gene:YJR003C(YJR003C_p)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YLR049C(YLR049C_p)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Putative protein of unknown function Gene:YLR281C(YLR281C_p)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene Gene:YMR160W(YMR160W_p)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YMR173W-A(YMR173W-A_d)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YNL170W(YNL170W_d)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR052C(YOR052C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YPR050C(YPR050C_d)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPS6(YIR039C)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance Gene:AIM41(YOR215C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:AMD1(YML035C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools Gene:ANB1(YJR047C)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication Gene:ARG2(YJL071W)|FD-Score:5.17|P-value:1.15E-7||SGD DESC:Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p Gene:ARG8(YOL140W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine Gene:ARO2(YGL148W)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATG8(YBL078C)|FD-Score:4.95|P-value:3.67E-7||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BUD3(YCL014W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:BUD6(YLR319C)|FD-Score:-3.21|P-value:6.55E-4||SGD DESC:Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate Gene:CBT1(YKL208W)|FD-Score:5.05|P-value:2.25E-7||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:COG1(YGL223C)|FD-Score:3.85|P-value:5.81E-5||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG5(YNL051W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX16(YJL003W)|FD-Score:-4.63|P-value:1.81E-6||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:COX23(YHR116W)|FD-Score:3.74|P-value:9.26E-5||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:CYB2(YML054C)|FD-Score:-3.78|P-value:7.83E-5||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:DCR2(YLR361C)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Phosphoesterase involved in downregulation of the unfolded protein response, at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START Gene:DEF1(YKL054C)|FD-Score:-3.2|P-value:6.77E-4||SGD DESC:RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis Gene:DFG16(YOR030W)|FD-Score:-3.79|P-value:7.53E-5||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DLD2(YDL178W)|FD-Score:3.18|P-value:7.27E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:DUG3(YNL191W)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:FAB1(YFR019W)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:FUI1(YBL042C)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:FYV6(YNL133C)|FD-Score:6.78|P-value:6.05E-12||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GRE2(YOL151W)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:GYP5(YPL249C)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:GYP8(YFL027C)|FD-Score:3.9|P-value:4.86E-5||SGD DESC:GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport Gene:HIS4(YCL030C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis Gene:HMT1(YBR034C)|FD-Score:3.79|P-value:7.41E-5||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HST2(YPL015C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HUA1(YGR268C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:HYR1(YIR037W)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:ISF1(YMR081C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant Gene:LSP1(YPL004C)|FD-Score:5.13|P-value:1.44E-7||SGD DESC:Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family Gene:MAM3(YOL060C)|FD-Score:6.77|P-value:6.64E-12||SGD DESC:Protein required for normal mitochondrial morphology, has similarity to hemolysins Gene:MAP2(YBL091C)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p Gene:MGR2(YPL098C)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MLP2(YIL149C)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length Gene:MMM1(YLL006W)|FD-Score:-4.31|P-value:8.22E-6||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MRM2(YGL136C)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ Gene:MRPL7(YDR237W)|FD-Score:3.75|P-value:8.83E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:NNT1(YLR285W)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination Gene:OAR1(YKL055C)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:OAZ1(YPL052W)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:PFS1(YHR185C)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PSR1(YLL010C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:RPL22B(YFL034C-A)|FD-Score:5.59|P-value:1.13E-8||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RPS19A(YOL121C)|FD-Score:-4.32|P-value:7.96E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:SDC25(YLL016W)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication Gene:SHO1(YER118C)|FD-Score:10.3|P-value:3.85E-25||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:SPO21(YOL091W)|FD-Score:-4.34|P-value:7.05E-6||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SRV2(YNL138W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:STE50(YCL032W)|FD-Score:3.85|P-value:5.82E-5||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SUN4(YNL066W)|FD-Score:3.81|P-value:7.04E-5||SGD DESC:Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family Gene:SUS1(YBR111W-A)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:TAE2(YPL009C)|FD-Score:5.19|P-value:1.04E-7||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:TAT1(YBR069C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TCB3(YML072C)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TCM62(YBR044C)|FD-Score:4.28|P-value:9.27E-6||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone Gene:TDA5(YLR426W_p)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:VMA1(YDL185W)|FD-Score:3.82|P-value:6.54E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA16(YHR026W)|FD-Score:4.35|P-value:6.81E-6||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VMA7(YGR020C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS25(YJR102C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS45(YGL095C)|FD-Score:3.9|P-value:4.86E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS52(YDR484W)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:YBL059W(YBL059W_p)|FD-Score:5.41|P-value:3.11E-8||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication Gene:YBL071C-B(YBL071C-B_p)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCL074W(YCL074W)|FD-Score:-4.19|P-value:1.40E-5||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YER130C(YER130C_p)|FD-Score:3.8|P-value:7.38E-5||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YFL013W-A(YFL013W-A_d)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YFL040W(YFL040W_p)|FD-Score:3.73|P-value:9.57E-5||SGD DESC:Putative transporter, member of the sugar porter family; YFL040W is not an essential gene Gene:YGL217C(YGL217C_d)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W Gene:YGR169C-A(YGR169C-A_p)|FD-Score:4.95|P-value:3.69E-7||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YHL044W(YHL044W_p)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YIL134C-A(YIL134C-A_p)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIL152W(YIL152W_p)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:Putative protein of unknown function Gene:YJL027C(YJL027C_p)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Putative protein of unknown function Gene:YJL144W(YJL144W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Cytoplasmic hydrophilin essential in dessication-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress Gene:YJR003C(YJR003C_p)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YLR049C(YLR049C_p)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Putative protein of unknown function Gene:YLR281C(YLR281C_p)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene Gene:YMR160W(YMR160W_p)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YMR173W-A(YMR173W-A_d)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YNL170W(YNL170W_d)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR052C(YOR052C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YPR050C(YPR050C_d)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPS6(YIR039C)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL173C6.757.24E-121.99RFA3Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
YMR076C4.779.31E-70.52PDS5Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis
YFR050C4.241.10E-50.11PRE4Beta 7 subunit of the 20S proteasome
YGL099W4.131.81E-50.68LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YKL196C3.452.78E-40.11YKT6Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus
YDL030W3.344.14E-40.04PRP9Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex
YLR197W3.304.79E-40.12NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YOL097C3.187.42E-40.18WRS1Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA
YBL041W3.000.001360.02PRE7Beta 6 subunit of the 20S proteasome
YMR239C2.980.001440.03RNT1Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes
YLR060W2.950.001600.08FRS1Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
YJL076W2.870.002050.01NET1Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication
YBL050W2.860.002150.02SEC17Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP
YEL002C2.840.002270.06WBP1Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum
YCL052C2.780.002710.00PBN1Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER118C10.303.85E-25SHO1Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p
YNL133C6.786.05E-12FYV6Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining
YOL060C6.776.64E-12MAM3Protein required for normal mitochondrial morphology, has similarity to hemolysins
YFL034C-A5.591.13E-8RPL22BRibosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication
YBL059W_p5.413.11E-8YBL059W_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YBL059W has a paralog, YER093C-A, that arose from the whole genome duplication
YPL009C5.191.04E-7TAE2Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YJL071W5.171.15E-7ARG2Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p
YPL004C5.131.44E-7LSP1Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family
YKL208W5.052.25E-7CBT1Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p
YBL078C4.953.67E-7ATG8Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis
YGR169C-A_p4.953.69E-7YGR169C-A_pPutative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication
YDR484W4.671.48E-6VPS52Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin
YHR026W4.356.81E-6VMA16Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain
YBR044C4.289.27E-6TCM62Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; putative chaperone
YBR111W-A4.261.01E-5SUS1Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP

GO enrichment analysis for SGTC_936
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0841.20E-10SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.095238160S ribosome export
0.0732.31E-8SGTC_29025787006 60.5 μMChembridge (Drug-like library)5910030.188406Golgi
0.0691.03E-7SGTC_13631636-0063 10.4 μMChemDiv (Drug-like library)60721850.0972222Golgi
0.0571.15E-5SGTC_2770toltrazuril 17.5 μMMiscellaneous685910.0843373
0.0561.50E-5SGTC_30389091290 49.5 μMChembridge (Drug-like library)252368430.12
0.0561.64E-5SGTC_24245537928 59.2 μMMiscellaneous53828020.0958904
0.0552.22E-5SGTC_23799035213 200.0 μMChembridge (Fragment library)6465510.114286
0.0552.70E-5SGTC_2830987-0079 11.7 μMChemDiv (Drug-like library)4115560.119403
0.0542.89E-5SGTC_9903985-0011 41.3 μMChemDiv (Drug-like library)53995650.15942amide catabolism
0.0543.08E-5SGTC_2840988-0104 32.6 μMChemDiv (Drug-like library)46665320.142857heme requiring
0.0543.16E-5SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.21951260S ribosome export
0.0543.55E-5SGTC_14780453-0656 35.7 μMChemDiv (Drug-like library)52584960.295082
0.0534.56E-5SGTC_28899059656 52.0 μMChembridge (Drug-like library)39498380.15625
0.0526.95E-5SGTC_15081080-0625 72.9 μMChemDiv (Drug-like library)7671110.132353
0.0501.20E-4SGTC_1025k007-0731 70.0 μMChemDiv (Drug-like library)62561180.15625

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3006907542471.43 μM0.5185197465181Chembridge (Drug-like library)334.160162.8314
SGTC_8261683-5028118 μM0.480769722377ChemDiv (Drug-like library)261.703583.26212
SGTC_3067911743149.47 μM0.47169817150885Chembridge (Drug-like library)285.337663.30813
SGTC_3250913558349.47 μM0.4444447699353Chembridge (Drug-like library)299.321182.80214
SGTC_20535265679138 μM0.442308764595Chembridge (Fragment library)233.286242.55113
SGTC_1090132-003633.7 μM0.41818267274ChemDiv (Drug-like library)293.31663.26423
SGTC_8971000-013931.4 μM0.3962266151020ChemDiv (Drug-like library)220.224580.54923
SGTC_9383126-1755342 μM0.3928571777771ChemDiv (Drug-like library)390.388723.01235
SGTC_2998906676271.43 μM0.3833334945196Chembridge (Drug-like library)297.348363.54713RPP1 & pyrimidine depletion
SGTC_9861493-048384 μM0.38181840079ChemDiv (Drug-like library)256.256681.31914