3122-0005

N-(4-methoxyphenyl)benzo[cd]indol-2-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_937
Screen concentration 46.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 759535
SMILES COC1=CC=C(C=C1)NC2=NC3=CC=CC4=C3C2=CC=C4
Standardized SMILES COc1ccc(NC2=Nc3cccc4cccc2c34)cc1
Molecular weight 274.3166
ALogP 3.51
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.19
% growth inhibition (Hom. pool) 10.78


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 759535
Download HIP data (tab-delimited text)  (excel)
Gene:ARC15(YIL062C)|FD-Score:4.26|P-value:1.01E-5|Clearance:0.56||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:CDC27(YBL084C)|FD-Score:-3.54|P-value:2.04E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC60(YPL160W)|FD-Score:-3.21|P-value:6.61E-4|Clearance:0||SGD DESC:Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Gene:MED4(YOR174W)|FD-Score:3.16|P-value:8.00E-4|Clearance:0.02||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MPP10(YJR002W)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.07||SGD DESC:Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p Gene:NOC4(YPR144C)|FD-Score:5.6|P-value:1.07E-8|Clearance:0.64||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:PAP1(YKR002W)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.3||SGD DESC:Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping Gene:PCF11(YDR228C)|FD-Score:3.16|P-value:8.00E-4|Clearance:0||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:QNS1(YHR074W)|FD-Score:3.33|P-value:4.30E-4|Clearance:0.18||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:SMC5(YOL034W)|FD-Score:3.13|P-value:8.66E-4|Clearance:0.12||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair Gene:SMT3(YDR510W)|FD-Score:5.36|P-value:4.13E-8|Clearance:0.64||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SPC105(YGL093W)|FD-Score:-3.14|P-value:8.50E-4|Clearance:0||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SRB7(YDR308C)|FD-Score:5.67|P-value:6.96E-9|Clearance:0.64||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p Gene:YPL238C(YPL238C_d)|FD-Score:4.73|P-value:1.15E-6|Clearance:0.46||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YTH1(YPR107C)|FD-Score:-3.31|P-value:4.72E-4|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ARC15(YIL062C)|FD-Score:4.26|P-value:1.01E-5|Clearance:0.56||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:CDC27(YBL084C)|FD-Score:-3.54|P-value:2.04E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC60(YPL160W)|FD-Score:-3.21|P-value:6.61E-4|Clearance:0||SGD DESC:Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Gene:MED4(YOR174W)|FD-Score:3.16|P-value:8.00E-4|Clearance:0.02||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MPP10(YJR002W)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.07||SGD DESC:Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p Gene:NOC4(YPR144C)|FD-Score:5.6|P-value:1.07E-8|Clearance:0.64||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:PAP1(YKR002W)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.3||SGD DESC:Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping Gene:PCF11(YDR228C)|FD-Score:3.16|P-value:8.00E-4|Clearance:0||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:QNS1(YHR074W)|FD-Score:3.33|P-value:4.30E-4|Clearance:0.18||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:SMC5(YOL034W)|FD-Score:3.13|P-value:8.66E-4|Clearance:0.12||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair Gene:SMT3(YDR510W)|FD-Score:5.36|P-value:4.13E-8|Clearance:0.64||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SPC105(YGL093W)|FD-Score:-3.14|P-value:8.50E-4|Clearance:0||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SRB7(YDR308C)|FD-Score:5.67|P-value:6.96E-9|Clearance:0.64||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p Gene:YPL238C(YPL238C_d)|FD-Score:4.73|P-value:1.15E-6|Clearance:0.46||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YTH1(YPR107C)|FD-Score:-3.31|P-value:4.72E-4|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 759535
Download HOP data (tab-delimited text)  (excel)
Gene:AIM26(YKL037W)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:APL1(YJR005W)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:ARO2(YGL148W)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATP14(YLR295C)|FD-Score:-5.05|P-value:2.17E-7||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:CAP1(YKL007W)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:COG7(YGL005C)|FD-Score:-4.33|P-value:7.47E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ10(YOL008W)|FD-Score:8.17|P-value:1.52E-16||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:CSF1(YLR087C)|FD-Score:6.63|P-value:1.64E-11||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CTR9(YOL145C)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DAL81(YIR023W)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DAL82(YNL314W)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:DBP7(YKR024C)|FD-Score:5.42|P-value:2.99E-8||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DMA2(YNL116W)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains Gene:ERV15(YBR210W)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication Gene:FKH2(YNL068C)|FD-Score:3.84|P-value:6.19E-5||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:GGA1(YDR358W)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GPB2(YAL056W)|FD-Score:-3.78|P-value:7.81E-5||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:GPR1(YDL035C)|FD-Score:5.25|P-value:7.57E-8||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HCH1(YNL281W)|FD-Score:4.45|P-value:4.33E-6||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:HIT1(YJR055W)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HOR7(YMR251W-A)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HXT3(YDR345C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication Gene:ICL2(YPR006C)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol Gene:IES3(YLR052W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IMP2'(YIL154C)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRS4(YKR019C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LEE1(YPL054W)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Zinc-finger protein of unknown function Gene:LNP1(YHR192W_p)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:LYS4(YDR234W)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MRP8(YKL142W)|FD-Score:-3.87|P-value:5.47E-5||SGD DESC:Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis Gene:NBP2(YDR162C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NUP133(YKR082W)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:OCA2(YNL056W)|FD-Score:5.28|P-value:6.58E-8||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:OYE3(YPL171C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:PDB1(YBR221C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PEX15(YOL044W)|FD-Score:4.33|P-value:7.30E-6||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PHO86(YJL117W)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PIF1(YML061C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PIH1(YHR034C)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:POT1(YIL160C)|FD-Score:4.43|P-value:4.76E-6||SGD DESC:3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids Gene:PPE1(YHR075C)|FD-Score:3.8|P-value:7.32E-5||SGD DESC:Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein Gene:PRM8(YGL053W)|FD-Score:-3.26|P-value:5.61E-4||SGD DESC:Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family Gene:PSK1(YAL017W)|FD-Score:3.79|P-value:7.41E-5||SGD DESC:PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication Gene:PTC1(YDL006W)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:RHB1(YCR027C)|FD-Score:-3.22|P-value:6.48E-4||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RIM15(YFL033C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase Gene:RKM3(YBR030W)|FD-Score:6.19|P-value:3.10E-10||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein Gene:ROM1(YGR070W)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:ROT2(YBR229C)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RPL19B(YBL027W)|FD-Score:4.47|P-value:3.89E-6||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RPL27A(YHR010W)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPS18B(YML026C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS6A(YPL090C)|FD-Score:6.35|P-value:1.09E-10||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:5.53|P-value:1.62E-8||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SAP30(YMR263W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SAP4(YGL229C)|FD-Score:-3.76|P-value:8.33E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SHP1(YBL058W)|FD-Score:-4.87|P-value:5.45E-7||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:TAT1(YBR069C)|FD-Score:4.35|P-value:6.94E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TCA17(YEL048C)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder Gene:TLG2(YOL018C)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPN1(YGL186C)|FD-Score:11.9|P-value:7.03E-33||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TPS2(YDR074W)|FD-Score:-4.39|P-value:5.55E-6||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:UBC5(YDR059C)|FD-Score:-3.15|P-value:8.17E-4||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:UBP3(YER151C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:YAL067W-A(YAL067W-A_p)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR064W(YBR064W_d)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YBR277C(YBR277C_d)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCL002C(YCL002C_p)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR085W(YCR085W_d)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR087C-A(YCR087C-A_p)|FD-Score:-3.1|P-value:9.69E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YCR100C(YCR100C_p)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Putative protein of unknown function Gene:YGR151C(YGR151C_d)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YHL041W(YHL041W_d)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YIH1(YCR059C)|FD-Score:4.06|P-value:2.41E-5||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YKL071W(YKL071W_p)|FD-Score:6.27|P-value:1.77E-10||SGD DESC:Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL115C(YKL115C_d)|FD-Score:-3.76|P-value:8.35E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YNL303W(YNL303W_d)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR025C(YNR025C_d)|FD-Score:5.79|P-value:3.57E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YOR034C-A(YOR034C-A_p)|FD-Score:4.3|P-value:8.64E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPK3(YBR028C)|FD-Score:-3.75|P-value:9.01E-5||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPL205C(YPL205C_d)|FD-Score:-3.36|P-value:3.96E-4||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:YPR014C(YPR014C_d)|FD-Score:5.35|P-value:4.40E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR050C(YPR050C_d)|FD-Score:5.2|P-value:9.85E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:AIM26(YKL037W)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:APL1(YJR005W)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:ARO2(YGL148W)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATP14(YLR295C)|FD-Score:-5.05|P-value:2.17E-7||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:CAP1(YKL007W)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:COG7(YGL005C)|FD-Score:-4.33|P-value:7.47E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ10(YOL008W)|FD-Score:8.17|P-value:1.52E-16||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:CSF1(YLR087C)|FD-Score:6.63|P-value:1.64E-11||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CTR9(YOL145C)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DAL81(YIR023W)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DAL82(YNL314W)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:DBP7(YKR024C)|FD-Score:5.42|P-value:2.99E-8||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DMA2(YNL116W)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains Gene:ERV15(YBR210W)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication Gene:FKH2(YNL068C)|FD-Score:3.84|P-value:6.19E-5||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:GGA1(YDR358W)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GPB2(YAL056W)|FD-Score:-3.78|P-value:7.81E-5||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:GPR1(YDL035C)|FD-Score:5.25|P-value:7.57E-8||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HCH1(YNL281W)|FD-Score:4.45|P-value:4.33E-6||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:HIT1(YJR055W)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HOR7(YMR251W-A)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HXT3(YDR345C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication Gene:ICL2(YPR006C)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol Gene:IES3(YLR052W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IMP2'(YIL154C)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRS4(YKR019C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LEE1(YPL054W)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Zinc-finger protein of unknown function Gene:LNP1(YHR192W_p)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:LYS4(YDR234W)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MRP8(YKL142W)|FD-Score:-3.87|P-value:5.47E-5||SGD DESC:Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis Gene:NBP2(YDR162C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NUP133(YKR082W)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:OCA2(YNL056W)|FD-Score:5.28|P-value:6.58E-8||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:OYE3(YPL171C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:PDB1(YBR221C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PEX15(YOL044W)|FD-Score:4.33|P-value:7.30E-6||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PHO86(YJL117W)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PIF1(YML061C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PIH1(YHR034C)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:POT1(YIL160C)|FD-Score:4.43|P-value:4.76E-6||SGD DESC:3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids Gene:PPE1(YHR075C)|FD-Score:3.8|P-value:7.32E-5||SGD DESC:Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein Gene:PRM8(YGL053W)|FD-Score:-3.26|P-value:5.61E-4||SGD DESC:Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family Gene:PSK1(YAL017W)|FD-Score:3.79|P-value:7.41E-5||SGD DESC:PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication Gene:PTC1(YDL006W)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:RHB1(YCR027C)|FD-Score:-3.22|P-value:6.48E-4||SGD DESC:Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins Gene:RIM15(YFL033C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase Gene:RKM3(YBR030W)|FD-Score:6.19|P-value:3.10E-10||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein Gene:ROM1(YGR070W)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:ROT2(YBR229C)|FD-Score:4.04|P-value:2.65E-5||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RPL19B(YBL027W)|FD-Score:4.47|P-value:3.89E-6||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RPL27A(YHR010W)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPS18B(YML026C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS6A(YPL090C)|FD-Score:6.35|P-value:1.09E-10||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:5.53|P-value:1.62E-8||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SAP30(YMR263W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SAP4(YGL229C)|FD-Score:-3.76|P-value:8.33E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SHP1(YBL058W)|FD-Score:-4.87|P-value:5.45E-7||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:TAT1(YBR069C)|FD-Score:4.35|P-value:6.94E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TCA17(YEL048C)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder Gene:TLG2(YOL018C)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPN1(YGL186C)|FD-Score:11.9|P-value:7.03E-33||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TPS2(YDR074W)|FD-Score:-4.39|P-value:5.55E-6||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:UBC5(YDR059C)|FD-Score:-3.15|P-value:8.17E-4||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:UBP3(YER151C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:YAL067W-A(YAL067W-A_p)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR064W(YBR064W_d)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YBR277C(YBR277C_d)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCL002C(YCL002C_p)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR085W(YCR085W_d)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR087C-A(YCR087C-A_p)|FD-Score:-3.1|P-value:9.69E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YCR100C(YCR100C_p)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Putative protein of unknown function Gene:YGR151C(YGR151C_d)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YHL041W(YHL041W_d)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YIH1(YCR059C)|FD-Score:4.06|P-value:2.41E-5||SGD DESC:Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT Gene:YKL071W(YKL071W_p)|FD-Score:6.27|P-value:1.77E-10||SGD DESC:Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL115C(YKL115C_d)|FD-Score:-3.76|P-value:8.35E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YNL303W(YNL303W_d)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR025C(YNR025C_d)|FD-Score:5.79|P-value:3.57E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase Gene:YOR034C-A(YOR034C-A_p)|FD-Score:4.3|P-value:8.64E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPK3(YBR028C)|FD-Score:-3.75|P-value:9.01E-5||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPL205C(YPL205C_d)|FD-Score:-3.36|P-value:3.96E-4||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:YPR014C(YPR014C_d)|FD-Score:5.35|P-value:4.40E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR050C(YPR050C_d)|FD-Score:5.2|P-value:9.85E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR308C5.676.96E-90.64SRB7Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p
YPR144C5.601.07E-80.64NOC4Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits
YDR510W5.364.13E-80.64SMT3Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2
YPL238C_d4.731.15E-60.46YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YIL062C4.261.01E-50.56ARC15Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity
YJR002W3.701.06E-40.07MPP10Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p
YKR002W3.631.41E-40.30PAP1Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping
YHR074W3.334.30E-40.18QNS1Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide
YDR228C3.168.00E-45.07E-5PCF11mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
YOR174W3.168.00E-40.02MED4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YOL034W3.138.66E-40.12SMC5Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair
YDR172W3.010.001310.03SUP35Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
YJR064W2.980.001430.07CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YJL019W2.910.001780.04MPS3Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
YJL032W_d2.870.002030.02YJL032W_dDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL186C11.907.03E-33TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YOL008W8.171.52E-16COQ10Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes
YLR087C6.631.64E-11CSF1Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YPL090C6.351.09E-10RPS6AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication
YKL071W_p6.271.77E-10YKL071W_pPutative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YBR030W6.193.10E-10RKM3Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein
YNR025C_d5.793.57E-9YNR025C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase
YOR014W5.531.62E-8RTS1B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A
YKR024C5.422.99E-8DBP7Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions
YPR014C_d5.354.40E-8YPR014C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene
YNL056W5.286.58E-8OCA2Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene
YDL035C5.257.57E-8GPR1Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis
YPR050C_d5.209.85E-8YPR050C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W
YMR251W-A4.552.74E-6HOR7Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication
YBL027W4.473.89E-6RPL19BRibosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication

GO enrichment analysis for SGTC_937
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1433.18E-28SGTC_9342950-0684 338.0 μMChemDiv (Drug-like library)11212530.183099
0.1301.12E-23SGTC_9383126-1755 342.0 μMChemDiv (Drug-like library)17777710.224138
0.1195.86E-20SGTC_7181130-0073 349.0 μMChemDiv (Drug-like library)67400200.106061
0.0952.30E-13SGTC_23307979373 200.0 μMChembridge (Fragment library)18630070.11111160S ribosome export
0.0939.22E-13SGTC_2525harmine 47.9 μMMiscellaneous52809530.192982ergosterol biosynthesis
0.0921.30E-12SGTC_7192810-4230 27.3 μMChemDiv (Drug-like library)7210790.078125
0.0898.89E-12SGTC_2545solidagenone 89.6 μMMicrosource (Natural product library)67085720.0512821
0.0881.41E-11SGTC_30969117468 49.5 μMChembridge (Drug-like library)413163110.0746269
0.0863.84E-11SGTC_24635473429 200.0 μMMiscellaneous53994050.0757576ERG2
0.0831.91E-10SGTC_477mead ethanolamide 10.0 μMICCB bioactive library160611850.0298507
0.0815.42E-10SGTC_9352996-0153 430.0 μMChemDiv (Drug-like library)237385230.078125PDR1
0.0807.86E-10SGTC_9903985-0011 41.3 μMChemDiv (Drug-like library)53995650.216667amide catabolism
0.0791.26E-9SGTC_30999120367 49.5 μMChembridge (Drug-like library)248249400.104478
0.0773.00E-9SGTC_464bw-b 70c 158.0 μMICCB bioactive library53534540.126761
0.0765.45E-9SGTC_413propylparaben 100.0 μMMiscellaneous71750.0892857amide catabolism

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_11423253-14089.78 μM0.354167256655ChemDiv (Drug-like library)276.332443.94123
SGTC_13833049-003220.7 μM0.339623712864ChemDiv (Drug-like library)291.347084.0261460S ribosome export
SGTC_12720839-002127 μM0.3061225027250ChemDiv (Drug-like library)210.2282.46802
SGTC_8730591-4978488 μM0.2982463305145ChemDiv (Drug-like library)300.309242.50815
SGTC_9433253-1379105 μM0.296296668464ChemDiv (Drug-like library)306.358423.92424
SGTC_14974476-297841.3 μM0.2857141528379ChemDiv (Drug-like library)331.310193.24317
SGTC_2877904072471.43 μM0.2857146463895Chembridge (Drug-like library)285.337663.30813
SGTC_13041165-0502251 μM0.283019767425ChemDiv (Drug-like library)289.34952.24314
SGTC_3065911388849.47 μM0.27419416648368Chembridge (Drug-like library)338.360522.91515
SGTC_7734358-145744.6 μM0.2698411186886ChemDiv (Drug-like library)352.38383.73716