0334-0113

1-[[[4-(1-adamantyl)-1,3-thiazol-2-yl]amino]methylidene]naphthalen-2-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_94
Screen concentration 514.8 μM
Source ChemDiv (Drug-like library)
PubChem CID 6795178
SMILES C1C2CC3CC1CC(C2)(C3)C4=CSC(=N4)NC=C5C(=O)C=CC6=CC=CC=C65
Standardized SMILES Oc1ccc2ccccc2c1C=Nc3nc(cs3)C45CC6CC(CC(C6)C4)C5
Molecular weight 388.5252
ALogP 4.99
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.71
% growth inhibition (Hom. pool) 5.05


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6795178
Download HIP data (tab-delimited text)  (excel)
Gene:NOP56(YLR197W)|FD-Score:5.07|P-value:2.02E-7|Clearance:0.76||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PAP1(YKR002W)|FD-Score:3.2|P-value:6.85E-4|Clearance:0.09||SGD DESC:Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping Gene:PWP2(YCR057C)|FD-Score:3.81|P-value:6.87E-5|Clearance:0.54||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RVB2(YPL235W)|FD-Score:4.58|P-value:2.36E-6|Clearance:0.76||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:UGP1(YKL035W)|FD-Score:4.87|P-value:5.49E-7|Clearance:0.76||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:ULP1(YPL020C)|FD-Score:3.11|P-value:9.38E-4|Clearance:0.05||SGD DESC:Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions Gene:UTP6(YDR449C)|FD-Score:3.28|P-value:5.26E-4|Clearance:0.08||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YIP1(YGR172C)|FD-Score:-4.59|P-value:2.25E-6|Clearance:0||SGD DESC:Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4 Gene:NOP56(YLR197W)|FD-Score:5.07|P-value:2.02E-7|Clearance:0.76||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PAP1(YKR002W)|FD-Score:3.2|P-value:6.85E-4|Clearance:0.09||SGD DESC:Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping Gene:PWP2(YCR057C)|FD-Score:3.81|P-value:6.87E-5|Clearance:0.54||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:RVB2(YPL235W)|FD-Score:4.58|P-value:2.36E-6|Clearance:0.76||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:UGP1(YKL035W)|FD-Score:4.87|P-value:5.49E-7|Clearance:0.76||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:ULP1(YPL020C)|FD-Score:3.11|P-value:9.38E-4|Clearance:0.05||SGD DESC:Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions Gene:UTP6(YDR449C)|FD-Score:3.28|P-value:5.26E-4|Clearance:0.08||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YIP1(YGR172C)|FD-Score:-4.59|P-value:2.25E-6|Clearance:0||SGD DESC:Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6795178
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:-3.99|P-value:3.33E-5||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AQY2(YLL052C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ASN2(YGR124W)|FD-Score:-3.27|P-value:5.42E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:ATG3(YNR007C)|FD-Score:4.34|P-value:7.09E-6||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:AVT2(YEL064C)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BGL2(YGR282C)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:CAJ1(YER048C)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CAP2(YIL034C)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CBR1(YIL043C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CTS1(YLR286C)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p Gene:DUG1(YFR044C)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EFM2(YBR271W)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis Gene:ERP6(YGL002W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication Gene:EST3(YIL009C-A)|FD-Score:3.86|P-value:5.72E-5||SGD DESC:Component of the telomerase holoenzyme, involved in telomere replication Gene:FET4(YMR319C)|FD-Score:4.99|P-value:3.02E-7||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FSF1(YOR271C_p)|FD-Score:-4.06|P-value:2.40E-5||SGD DESC:Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis Gene:FUR4(YBR021W)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GAL10(YBR019C)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers Gene:GCG1(YER163C_p)|FD-Score:4.31|P-value:7.99E-6||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:ICT1(YLR099C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:IOC4(YMR044W)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:LOC1(YFR001W)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:LYS1(YIR034C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MED1(YPR070W)|FD-Score:4.82|P-value:7.35E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MND2(YIR025W)|FD-Score:7|P-value:1.25E-12||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MSH1(YHR120W)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MTC1(YJL123C)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1 Gene:NTH2(YBR001C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PAU7(YAR020C)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme Gene:PCL1(YNL289W)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PEX12(YMR026C)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PRM2(YIL037C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion Gene:RAD27(YKL113C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RBK1(YCR036W)|FD-Score:3.97|P-value:3.61E-5||SGD DESC:Putative ribokinase Gene:RDR1(YOR380W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins Gene:ROY1(YMR258C)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPS1A(YLR441C)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:SDL1(YIL167W)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase Gene:SFA1(YDL168W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SKM1(YOL113W)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SPO73(YER046W)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis Gene:SPS18(YNL204C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SPS19(YNL202W)|FD-Score:5.29|P-value:6.01E-8||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SPT2(YER161C)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SRL3(YKR091W)|FD-Score:6.65|P-value:1.43E-11||SGD DESC:Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate Gene:SSQ1(YLR369W)|FD-Score:-4.35|P-value:6.71E-6||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:THI2(YBR240C)|FD-Score:4.49|P-value:3.60E-6||SGD DESC:Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type Gene:VMA11(YPL234C)|FD-Score:4.28|P-value:9.53E-6||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VMR1(YHL035C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VPS64(YDR200C)|FD-Score:3.98|P-value:3.47E-5||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:YBR016W(YBR016W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YBR056W(YBR056W_p)|FD-Score:5.35|P-value:4.39E-8||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR220C(YBR220C_p)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Putative protein of unknown function; YBR220C is not an essential gene Gene:YGL007C-A(YGL007C-A_p)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YGR039W(YGR039W_d)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722 Gene:YGR228W(YGR228W_d)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YIL108W(YIL108W_p)|FD-Score:-3.1|P-value:9.77E-4||SGD DESC:Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress Gene:YLL056C(YLL056C_p)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin Gene:YLR184W(YLR184W_d)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML018C(YML018C_p)|FD-Score:4.55|P-value:2.63E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YML047W-A(YML047W-A_d)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR155W(YMR155W_p)|FD-Score:-4.53|P-value:2.89E-6||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YMR173W-A(YMR173W-A_d)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YMR310C(YMR310C_p)|FD-Score:6.35|P-value:1.08E-10||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNL235C(YNL235C_d)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOL013W-A(YOL013W-A_p)|FD-Score:5.27|P-value:6.72E-8||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YOL098C(YOL098C_p)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Putative metalloprotease Gene:YOL118C(YOL118C_d)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR059C(YOR059C_p)|FD-Score:-3.53|P-value:2.12E-4||SGD DESC:Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress Gene:YOR199W(YOR199W_d)|FD-Score:-6.15|P-value:3.91E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPK3(YBR028C)|FD-Score:5.25|P-value:7.63E-8||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPR098C(YPR098C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPR172W(YPR172W_p)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication Gene:YTP1(YNL237W)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ADO1(YJR105W)|FD-Score:-3.99|P-value:3.33E-5||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AQY2(YLL052C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ASN2(YGR124W)|FD-Score:-3.27|P-value:5.42E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:ATG3(YNR007C)|FD-Score:4.34|P-value:7.09E-6||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:AVT2(YEL064C)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BGL2(YGR282C)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:CAJ1(YER048C)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CAP2(YIL034C)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CBR1(YIL043C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CTS1(YLR286C)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Endochitinase, required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p Gene:DUG1(YFR044C)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EFM2(YBR271W)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis Gene:ERP6(YGL002W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication Gene:EST3(YIL009C-A)|FD-Score:3.86|P-value:5.72E-5||SGD DESC:Component of the telomerase holoenzyme, involved in telomere replication Gene:FET4(YMR319C)|FD-Score:4.99|P-value:3.02E-7||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FSF1(YOR271C_p)|FD-Score:-4.06|P-value:2.40E-5||SGD DESC:Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis Gene:FUR4(YBR021W)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GAL10(YBR019C)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:UDP-glucose-4-epimerase, catalyzes the interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes the conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers Gene:GCG1(YER163C_p)|FD-Score:4.31|P-value:7.99E-6||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:ICT1(YLR099C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:IOC4(YMR044W)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:LOC1(YFR001W)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:LYS1(YIR034C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MED1(YPR070W)|FD-Score:4.82|P-value:7.35E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MND2(YIR025W)|FD-Score:7|P-value:1.25E-12||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MSH1(YHR120W)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MTC1(YJL123C)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1 Gene:NTH2(YBR001C)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:PAU7(YAR020C)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Member of the seripauperin multigene family, active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme Gene:PCL1(YNL289W)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PEX12(YMR026C)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PRM2(YIL037C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion Gene:RAD27(YKL113C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RBK1(YCR036W)|FD-Score:3.97|P-value:3.61E-5||SGD DESC:Putative ribokinase Gene:RDR1(YOR380W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins Gene:ROY1(YMR258C)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPS1A(YLR441C)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:SDL1(YIL167W)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL167W and adjacent ORF, YIL168W, constitute a single ORF encoding L-serine dehydratase Gene:SFA1(YDL168W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SKM1(YOL113W)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SPO73(YER046W)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensible for both nuclear divisions during meiosis Gene:SPS18(YNL204C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SPS19(YNL202W)|FD-Score:5.29|P-value:6.01E-8||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SPT2(YER161C)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SRL3(YKR091W)|FD-Score:6.65|P-value:1.43E-11||SGD DESC:Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate Gene:SSQ1(YLR369W)|FD-Score:-4.35|P-value:6.71E-6||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:THI2(YBR240C)|FD-Score:4.49|P-value:3.60E-6||SGD DESC:Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type Gene:VMA11(YPL234C)|FD-Score:4.28|P-value:9.53E-6||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VMR1(YHL035C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VPS64(YDR200C)|FD-Score:3.98|P-value:3.47E-5||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:YBR016W(YBR016W)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication Gene:YBR056W(YBR056W_p)|FD-Score:5.35|P-value:4.39E-8||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR220C(YBR220C_p)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Putative protein of unknown function; YBR220C is not an essential gene Gene:YGL007C-A(YGL007C-A_p)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YGR039W(YGR039W_d)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722 Gene:YGR228W(YGR228W_d)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YIL108W(YIL108W_p)|FD-Score:-3.1|P-value:9.77E-4||SGD DESC:Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress Gene:YLL056C(YLL056C_p)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Putative protein of unknown function, transcription is activated by paralogous transcription factors Yrm1p and Yrr1p and genes involved in pleiotropic drug resistance (PDR); expression is induced in cells treated with the mycotoxin patulin Gene:YLR184W(YLR184W_d)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML018C(YML018C_p)|FD-Score:4.55|P-value:2.63E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YML047W-A(YML047W-A_d)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR155W(YMR155W_p)|FD-Score:-4.53|P-value:2.89E-6||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen Gene:YMR173W-A(YMR173W-A_d)|FD-Score:-3.54|P-value:1.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YMR310C(YMR310C_p)|FD-Score:6.35|P-value:1.08E-10||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNL235C(YNL235C_d)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOL013W-A(YOL013W-A_p)|FD-Score:5.27|P-value:6.72E-8||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YOL098C(YOL098C_p)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Putative metalloprotease Gene:YOL118C(YOL118C_d)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR059C(YOR059C_p)|FD-Score:-3.53|P-value:2.12E-4||SGD DESC:Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress Gene:YOR199W(YOR199W_d)|FD-Score:-6.15|P-value:3.91E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPK3(YBR028C)|FD-Score:5.25|P-value:7.63E-8||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPR098C(YPR098C)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPR172W(YPR172W_p)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication Gene:YTP1(YNL237W)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR197W5.072.02E-70.76NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YKL035W4.875.49E-70.76UGP1UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
YPL235W4.582.36E-60.76RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YCR057C3.816.87E-50.54PWP2Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis
YDR449C3.285.26E-40.08UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YKR002W3.206.85E-40.09PAP1Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping
YPL020C3.119.38E-40.05ULP1Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions
YDR429C3.060.001120.07TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YIL144W2.990.001400.10TID3Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
YGR048W2.890.001950.10UFD1Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
YPR136C_d2.790.002650.03YPR136C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
YFL022C2.750.002951.88E-4FRS2Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
YKL180W2.750.002950.04RPL17ARibosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YGR191W2.710.003350.01HIP1High-affinity histidine permease, also involved in the transport of manganese ions
YGL068W2.710.003410.07MNP1Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIR025W7.001.25E-12MND2Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase
YKR091W6.651.43E-11SRL3Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate
YMR310C_p6.351.08E-10YMR310C_pPutative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene
YBR056W_p5.354.39E-8YBR056W_pPutative glycoside hydrolase of the mitochondrial intermembrane space
YNL202W5.296.01E-8SPS19Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate
YOL013W-A_p5.276.72E-8YOL013W-A_pPutative protein of unknown function; identified by SAGE
YBR028C5.257.63E-8YPK3An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner
YMR319C4.993.02E-7FET4Low-affinity Fe(II) transporter of the plasma membrane
YPR070W4.827.35E-7MED1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YLR441C4.751.01E-6RPS1ARibosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication
YML018C_p4.552.63E-6YML018C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication
YBR240C4.493.60E-6THI2Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type
YNR007C4.347.09E-6ATG3E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p
YER163C_p4.317.99E-6GCG1_pGamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle
YPL234C4.289.53E-6VMA11Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen

GO enrichment analysis for SGTC_94
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1195.48E-20SGTC_920063-0024 660.9 μMChemDiv (Drug-like library)35944370.0571429
0.0723.51E-8SGTC_90092-0002 10.4 μMChemDiv (Drug-like library)7593680.0714286
0.0691.22E-7SGTC_950471-0001 561.1 μMChemDiv (Drug-like library)40154050.0921053
0.0663.62E-7SGTC_2234tolnaftate 200.0 μMMiscellaneous55100.103896
0.0664.31E-7SGTC_12650819-1001 68.8 μMChemDiv (Drug-like library)31067680.0375
0.0596.53E-6SGTC_8570438-0306 1.4 μMChemDiv (Drug-like library)46861360.0151515copper-dependent oxidative stress
0.0571.30E-5SGTC_1453737-0113 38.4 μMChemDiv (Drug-like library)68493510.142857DNA intercalators
0.0552.36E-5SGTC_1471k673-0312 37.0 μMChemDiv (Drug-like library)240998840.0454545RNA processing & uracil transport
0.0533.95E-5SGTC_29999069765 71.4 μMChembridge (Drug-like library)20970660.0493827amide catabolism
0.0534.92E-5SGTC_1938st074717 56.4 μMTimTec (Natural product derivative library)172509790.0444444
0.0528.65E-5SGTC_1461k072-0203 53.8 μMChemDiv (Drug-like library)54187140.125
0.0491.46E-4SGTC_14863970-0793 4.5 μMChemDiv (Drug-like library)28443300.0555556
0.0491.54E-4SGTC_21345324836 1.5 μMChembridge (Fragment library)6992720.0571429
0.0482.12E-4SGTC_1926methyl fluorone black 53.6 μMTimTec (Natural product derivative library)727210.0657895DNA intercalators
0.0482.30E-4SGTC_23047443016 91.4 μMChembridge (Fragment library)9398540.0769231

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_12450418-007617.2 μM0.593226790114ChemDiv (Drug-like library)417.362544.38714ERG2
SGTC_240417-1663219 μM0.4558824329390ChemDiv (Drug-like library)456.599126.54705
SGTC_8230417-1665178 μM0.4492753429856ChemDiv (Drug-like library)450.679128.39804
SGTC_7181130-0073349 μM0.4098366740020ChemDiv (Drug-like library)282.724343.20213
SGTC_780336-0123156.75 μM0.4090916765896ChemDiv (Drug-like library)380.461645.15814
SGTC_6441498-119763.3 μM0.3934436741615ChemDiv (Drug-like library)291.343782.86623
SGTC_6671498-104459 μM0.3934436739982ChemDiv (Drug-like library)291.300722.77924RPP1 & pyrimidine depletion
SGTC_6360kpi-0074126 μM0.388889616434ChemDiv (Drug-like library)234.360422.91813
SGTC_7851270-011165.3 μM0.3809526741994ChemDiv (Drug-like library)307.300122.53735
SGTC_1280906-298113.37 μM0.3636366740226ChemDiv (Drug-like library)296.343682.0124