1252-1571

methyl N'-[(3-bromo-6-oxocyclohexa-2,4-dien-1-ylidene)methylamino]carbamimidothioate

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_961
Screen concentration 372.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 6813498
SMILES CSC(=NNC=C1C=C(C=CC1=O)Br)N
Standardized SMILES CSC(=N)NN=Cc1cc(Br)ccc1O
Molecular weight 288.1642
ALogP 1.05
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 25
% growth inhibition (Hom. pool) 10.24


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6813498
Download HIP data (tab-delimited text)  (excel)
Gene:ABF1(YKL112W)|FD-Score:4.46|P-value:4.18E-6|Clearance:0.55||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:ARC15(YIL062C)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.01||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:DBP2(YNL112W)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.13||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:FCP1(YMR277W)|FD-Score:3.12|P-value:9.05E-4|Clearance:0.01||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:GLN4(YOR168W)|FD-Score:3.21|P-value:6.56E-4|Clearance:0.08||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:JIP5(YPR169W)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.02||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:NOP56(YLR197W)|FD-Score:3.14|P-value:8.58E-4|Clearance:0.02||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NUP192(YJL039C)|FD-Score:-3.4|P-value:3.42E-4|Clearance:0||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:PDS5(YMR076C)|FD-Score:-3.28|P-value:5.23E-4|Clearance:0||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PRP43(YGL120C)|FD-Score:3.49|P-value:2.40E-4|Clearance:0.28||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:RET1(YOR207C)|FD-Score:3.11|P-value:9.43E-4|Clearance:0.05||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RHO1(YPR165W)|FD-Score:-4.98|P-value:3.15E-7|Clearance:0||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPB3(YIL021W)|FD-Score:3.52|P-value:2.19E-4|Clearance:0||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:SEC16(YPL085W)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.01||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:SEC2(YNL272C)|FD-Score:3.91|P-value:4.64E-5|Clearance:0.09||SGD DESC:Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles Gene:SUB2(YDL084W)|FD-Score:-3.14|P-value:8.37E-4|Clearance:0||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TAF12(YDR145W)|FD-Score:5.57|P-value:1.29E-8|Clearance:1.11||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:YOR102W(YOR102W_d)|FD-Score:-3.31|P-value:4.67E-4|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex Gene:YRA1(YDR381W)|FD-Score:3.82|P-value:6.58E-5|Clearance:0.15||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member Gene:ABF1(YKL112W)|FD-Score:4.46|P-value:4.18E-6|Clearance:0.55||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:ARC15(YIL062C)|FD-Score:3.53|P-value:2.11E-4|Clearance:0.01||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity Gene:DBP2(YNL112W)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.13||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:FCP1(YMR277W)|FD-Score:3.12|P-value:9.05E-4|Clearance:0.01||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:GLN4(YOR168W)|FD-Score:3.21|P-value:6.56E-4|Clearance:0.08||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:JIP5(YPR169W)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.02||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:NOP56(YLR197W)|FD-Score:3.14|P-value:8.58E-4|Clearance:0.02||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NUP192(YJL039C)|FD-Score:-3.4|P-value:3.42E-4|Clearance:0||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:PDS5(YMR076C)|FD-Score:-3.28|P-value:5.23E-4|Clearance:0||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:PRP43(YGL120C)|FD-Score:3.49|P-value:2.40E-4|Clearance:0.28||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:RET1(YOR207C)|FD-Score:3.11|P-value:9.43E-4|Clearance:0.05||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RHO1(YPR165W)|FD-Score:-4.98|P-value:3.15E-7|Clearance:0||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPB3(YIL021W)|FD-Score:3.52|P-value:2.19E-4|Clearance:0||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:SEC16(YPL085W)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.01||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:SEC2(YNL272C)|FD-Score:3.91|P-value:4.64E-5|Clearance:0.09||SGD DESC:Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles Gene:SUB2(YDL084W)|FD-Score:-3.14|P-value:8.37E-4|Clearance:0||SGD DESC:Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:TAF12(YDR145W)|FD-Score:5.57|P-value:1.29E-8|Clearance:1.11||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:YOR102W(YOR102W_d)|FD-Score:-3.31|P-value:4.67E-4|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex Gene:YRA1(YDR381W)|FD-Score:3.82|P-value:6.58E-5|Clearance:0.15||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6813498
Download HOP data (tab-delimited text)  (excel)
Gene:APL2(YKL135C)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:AQY2(YLL052C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ARO1(YDR127W)|FD-Score:4.37|P-value:6.25E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG8(YBL078C)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:CLC1(YGR167W)|FD-Score:5.2|P-value:1.01E-7||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CLD1(YGR110W)|FD-Score:5.77|P-value:3.93E-9||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:CMP2(YML057W)|FD-Score:5.27|P-value:6.99E-8||SGD DESC:Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:CNB1(YKL190W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:CRS5(YOR031W)|FD-Score:4.35|P-value:6.82E-6||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:DAL81(YIR023W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:EAF1(YDR359C)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:EAR1(YMR171C)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:EDE1(YBL047C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:EMC3(YKL207W)|FD-Score:4.48|P-value:3.78E-6||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 Gene:FUI1(YBL042C)|FD-Score:5.51|P-value:1.82E-8||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GAP1(YKR039W)|FD-Score:6.5|P-value:4.14E-11||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GFD1(YMR255W)|FD-Score:-4.84|P-value:6.52E-7||SGD DESC:Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress Gene:GIN4(YDR507C)|FD-Score:3.73|P-value:9.73E-5||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:GPH1(YPR160W)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Non-essential glycogen phosphorylase required for the mobilization of glycogen, activity is regulated by cyclic AMP-mediated phosphorylation, expression is regulated by stress-response elements and by the HOG MAP kinase pathway Gene:GSF2(YML048W)|FD-Score:-4|P-value:3.13E-5||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:HDA3(YPR179C)|FD-Score:-5.38|P-value:3.79E-8||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p Gene:HOM3(YER052C)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HSV2(YGR223C)|FD-Score:5.52|P-value:1.69E-8||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:IES4(YOR189W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Component of the INO80 chromatiin remodeling complex and target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses Gene:IRC25(YLR021W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:IRC3(YDR332W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:IRC8(YJL051W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc Gene:LRS4(YDR439W)|FD-Score:4.91|P-value:4.45E-7||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MCT1(YOR221C)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MEK1(YOR351C)|FD-Score:6.38|P-value:8.86E-11||SGD DESC:Meiosis-specific serine/threonine protein kinase, functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids Gene:MFM1(YPL060W)|FD-Score:-3.97|P-value:3.53E-5||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MMT2(YPL224C)|FD-Score:-3.35|P-value:4.07E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MND2(YIR025W)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MPA43(YNL249C)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NMA111(YNL123W)|FD-Score:5.77|P-value:4.07E-9||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:OPI1(YHL020C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:OPI8(YKR035C_d)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:PET309(YLR067C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PEX25(YPL112C)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Peripheral peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with homologous protein Pex27p Gene:PMD1(YER132C)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:PPT2(YPL148C)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:RAD9(YDR217C)|FD-Score:-4.42|P-value:5.03E-6||SGD DESC:DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate Gene:RCE1(YMR274C)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RML2(YEL050C)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL21B(YPL079W)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:3.78|P-value:7.73E-5||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPS18A(YDR450W)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RTS3(YGR161C_p)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SAC3(YDR159W)|FD-Score:6.49|P-value:4.17E-11||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SCH9(YHR205W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan Gene:SGO1(YOR073W)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SIA1(YOR137C)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SLX9(YGR081C)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:STB2(YMR053C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:STP1(YDR463W)|FD-Score:4.84|P-value:6.43E-7||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SWS2(YNL081C)|FD-Score:4.4|P-value:5.35E-6||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TAT1(YBR069C)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TCA17(YEL048C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder Gene:TMA19(YKL056C)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TRM732(YMR259C_p)|FD-Score:3.85|P-value:5.87E-5||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:TRM9(YML014W)|FD-Score:5.94|P-value:1.46E-9||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:TRP2(YER090W)|FD-Score:6.78|P-value:6.17E-12||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP4(YDR354W)|FD-Score:8.69|P-value:1.75E-18||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UGA2(YBR006W)|FD-Score:7.88|P-value:1.67E-15||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VPS63(YLR261C_d)|FD-Score:3.82|P-value:6.77E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBL071C-B(YBL071C-B_p)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCL075W(YCL075W)|FD-Score:4.3|P-value:8.51E-6||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDR008C(YDR008C_d)|FD-Score:5.63|P-value:9.08E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR239C(YDR239C)|FD-Score:-4.88|P-value:5.43E-7||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YDR431W(YDR431W_d)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR535C(YDR535C_d)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YER079W(YER079W_p)|FD-Score:4.4|P-value:5.46E-6||SGD DESC:Putative protein of unknown function Gene:YER135C(YER135C_d)|FD-Score:-3.2|P-value:6.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YGL010W(YGL010W_p)|FD-Score:3.89|P-value:4.91E-5||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGL117W(YGL117W_p)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Putative protein of unknown function Gene:YGR122C-A(YGR122C-A_d)|FD-Score:-3.17|P-value:7.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Gene:YMR316C-B(YMR316C-B_d)|FD-Score:12.1|P-value:9.02E-34||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL170W(YNL170W_d)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR062C(YNR062C_p)|FD-Score:-3.87|P-value:5.51E-5||SGD DESC:Putative membrane protein of unknown function Gene:YOR1(YGR281W)|FD-Score:-3.2|P-value:6.84E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YOR223W(YOR223W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YVH1(YIR026C)|FD-Score:3.81|P-value:6.99E-5||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases Gene:APL2(YKL135C)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:AQY2(YLL052C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ARO1(YDR127W)|FD-Score:4.37|P-value:6.25E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG8(YBL078C)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:CLC1(YGR167W)|FD-Score:5.2|P-value:1.01E-7||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CLD1(YGR110W)|FD-Score:5.77|P-value:3.93E-9||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:CMP2(YML057W)|FD-Score:5.27|P-value:6.99E-8||SGD DESC:Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:CNB1(YKL190W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:CRS5(YOR031W)|FD-Score:4.35|P-value:6.82E-6||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:DAL81(YIR023W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:EAF1(YDR359C)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:EAR1(YMR171C)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p Gene:EDE1(YBL047C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:EMC3(YKL207W)|FD-Score:4.48|P-value:3.78E-6||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 Gene:FUI1(YBL042C)|FD-Score:5.51|P-value:1.82E-8||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:GAP1(YKR039W)|FD-Score:6.5|P-value:4.14E-11||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GFD1(YMR255W)|FD-Score:-4.84|P-value:6.52E-7||SGD DESC:Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress Gene:GIN4(YDR507C)|FD-Score:3.73|P-value:9.73E-5||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:GPH1(YPR160W)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Non-essential glycogen phosphorylase required for the mobilization of glycogen, activity is regulated by cyclic AMP-mediated phosphorylation, expression is regulated by stress-response elements and by the HOG MAP kinase pathway Gene:GSF2(YML048W)|FD-Score:-4|P-value:3.13E-5||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:HDA3(YPR179C)|FD-Score:-5.38|P-value:3.79E-8||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p Gene:HOM3(YER052C)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HSV2(YGR223C)|FD-Score:5.52|P-value:1.69E-8||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:IES4(YOR189W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Component of the INO80 chromatiin remodeling complex and target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses Gene:IRC25(YLR021W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:IRC3(YDR332W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:IRC8(YJL051W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc Gene:LRS4(YDR439W)|FD-Score:4.91|P-value:4.45E-7||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MCT1(YOR221C)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MEK1(YOR351C)|FD-Score:6.38|P-value:8.86E-11||SGD DESC:Meiosis-specific serine/threonine protein kinase, functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids Gene:MFM1(YPL060W)|FD-Score:-3.97|P-value:3.53E-5||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MMT2(YPL224C)|FD-Score:-3.35|P-value:4.07E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MND2(YIR025W)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MPA43(YNL249C)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NMA111(YNL123W)|FD-Score:5.77|P-value:4.07E-9||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:OPI1(YHL020C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:OPI8(YKR035C_d)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:PET309(YLR067C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PEX25(YPL112C)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Peripheral peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with homologous protein Pex27p Gene:PMD1(YER132C)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:PPT2(YPL148C)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:RAD9(YDR217C)|FD-Score:-4.42|P-value:5.03E-6||SGD DESC:DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate Gene:RCE1(YMR274C)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RML2(YEL050C)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL21B(YPL079W)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:3.78|P-value:7.73E-5||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPS18A(YDR450W)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RTS3(YGR161C_p)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SAC3(YDR159W)|FD-Score:6.49|P-value:4.17E-11||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SCH9(YHR205W)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan Gene:SGO1(YOR073W)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SIA1(YOR137C)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SLX9(YGR081C)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:STB2(YMR053C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:STP1(YDR463W)|FD-Score:4.84|P-value:6.43E-7||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SWS2(YNL081C)|FD-Score:4.4|P-value:5.35E-6||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TAT1(YBR069C)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TCA17(YEL048C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder Gene:TMA19(YKL056C)|FD-Score:3.18|P-value:7.24E-4||SGD DESC:Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress Gene:TRM732(YMR259C_p)|FD-Score:3.85|P-value:5.87E-5||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:TRM9(YML014W)|FD-Score:5.94|P-value:1.46E-9||SGD DESC:tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses Gene:TRP2(YER090W)|FD-Score:6.78|P-value:6.17E-12||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP4(YDR354W)|FD-Score:8.69|P-value:1.75E-18||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UGA2(YBR006W)|FD-Score:7.88|P-value:1.67E-15||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VPS63(YLR261C_d)|FD-Score:3.82|P-value:6.77E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBL071C-B(YBL071C-B_p)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCL075W(YCL075W)|FD-Score:4.3|P-value:8.51E-6||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDR008C(YDR008C_d)|FD-Score:5.63|P-value:9.08E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR239C(YDR239C)|FD-Score:-4.88|P-value:5.43E-7||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YDR431W(YDR431W_d)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR535C(YDR535C_d)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YER079W(YER079W_p)|FD-Score:4.4|P-value:5.46E-6||SGD DESC:Putative protein of unknown function Gene:YER135C(YER135C_d)|FD-Score:-3.2|P-value:6.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YGL010W(YGL010W_p)|FD-Score:3.89|P-value:4.91E-5||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YGL117W(YGL117W_p)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Putative protein of unknown function Gene:YGR122C-A(YGR122C-A_d)|FD-Score:-3.17|P-value:7.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Gene:YMR316C-B(YMR316C-B_d)|FD-Score:12.1|P-value:9.02E-34||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL170W(YNL170W_d)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR062C(YNR062C_p)|FD-Score:-3.87|P-value:5.51E-5||SGD DESC:Putative membrane protein of unknown function Gene:YOR1(YGR281W)|FD-Score:-3.2|P-value:6.84E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YOR223W(YOR223W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YVH1(YIR026C)|FD-Score:3.81|P-value:6.99E-5||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR145W5.571.29E-81.11TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YKL112W4.464.18E-60.55ABF1DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair
YNL272C3.914.64E-50.09SEC2Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles
YDR381W3.826.58E-50.15YRA1RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member
YNL112W3.671.20E-40.13DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YPL085W3.542.00E-40.01SEC16COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p
YIL062C3.532.11E-40.01ARC15Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; has mRNA binding activity
YIL021W3.522.19E-40.00RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
YPR169W3.512.21E-40.02JIP5Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein
YGL120C3.492.40E-40.28PRP43RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome
YOR168W3.216.56E-40.08GLN4Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
YLR197W3.148.58E-40.02NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YMR277W3.129.05E-40.01FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YOR207C3.119.43E-40.05RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YKL078W3.060.001120.03DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR316C-B_d12.109.02E-34YMR316C-B_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR354W8.691.75E-18TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YBR006W7.881.67E-15UGA2Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
YER090W6.786.17E-12TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YKR039W6.504.14E-11GAP1General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth
YDR159W6.494.17E-11SAC3Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP)
YOR351C6.388.86E-11MEK1Meiosis-specific serine/threonine protein kinase, functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids
YML014W5.941.46E-9TRM9tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
YGR110W5.773.93E-9CLD1Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58
YNL123W5.774.07E-9NMA111Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
YDR008C_d5.639.08E-9YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YGR223C5.521.69E-8HSV2Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization
YBL042C5.511.82E-8FUI1High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
YML057W5.276.99E-8CMP2Calcineurin A; one isoform (the other is CNA1) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1
YGR167W5.201.01E-7CLC1Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W

GO enrichment analysis for SGTC_961
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1924.83E-50SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.047619
0.1845.39E-46SGTC_2733amorolfine 100.0 μMMiscellaneous542600.0571429plasma membrane duress
0.1817.00E-45SGTC_9332914-0560 114.0 μMChemDiv (Drug-like library)4638870.0483871
0.1717.86E-40SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.057971
0.1697.33E-39SGTC_14964469-0713 57.4 μMChemDiv (Drug-like library)7466910.0533333
0.1653.12E-37SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.117647
0.1631.45E-36SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.057971
0.1632.00E-36SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.114754
0.1612.04E-35SGTC_9601222-0186 7.7 μMChemDiv (Drug-like library)31262960.0694444
0.1591.49E-34SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0454545
0.1583.34E-34SGTC_505gw-5074 63.9 μMICCB bioactive library59242080.140625
0.1553.08E-33SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.188679
0.1541.10E-32SGTC_24725377652 49.4 μMMiscellaneous16109030.09375TRP & mitochondrial translation
0.1532.53E-32SGTC_2735sertaconazole 140.0 nMMiscellaneous658630.0470588plasma membrane duress
0.1511.68E-31SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.0169492

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_12500485-034940.2 μM0.4081636842794ChemDiv (Drug-like library)334.25082.95412
SGTC_3031348-166834.2 μM0.3846155380509ChemDiv (Drug-like library)292.13131.61324
SGTC_7560508-199928.3 μM0.379316753853ChemDiv (Drug-like library)394.261244.44213RPP1 & pyrimidine depletion
SGTC_13221348-107012 μM0.3606566758089ChemDiv (Drug-like library)402.308143.54224
SGTC_6420335-0876158 μM0.3448286812350ChemDiv (Drug-like library)424.373345.41712ubiquinone biosynthesis & proteasome
SGTC_310508-197457.24 μM0.3333336810525ChemDiv (Drug-like library)414.679724.6213
SGTC_1320417-171525.81 μM0.3333336769098ChemDiv (Drug-like library)409.299024.56114RPP1 & pyrimidine depletion
SGTC_3301488-031980.1 μM0.3333336846614ChemDiv (Drug-like library)392.248663.99223RPP1 & pyrimidine depletion
SGTC_12450418-007617.2 μM0.3278696790114ChemDiv (Drug-like library)417.362544.38714ERG2
SGTC_910417-175158.38 μM0.3225816795624ChemDiv (Drug-like library)409.299024.56114Golgi