1254-0300

N-[(E)-1-(5-bromofuran-2-yl)ethylideneamino]-2-(2,4-dichlorophenoxy)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_962
Screen concentration 21.5 μM
Source ChemDiv (Drug-like library)
PubChem CID 7340576
SMILES CC(=NNC(=O)COC1=C(C=C(C=C1)Cl)Cl)C2=CC=C(O2)Br
Standardized SMILES CC(=NNC(=O)COc1ccc(Cl)cc1Cl)c2oc(Br)cc2
Molecular weight 406.0587
ALogP 3.79
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.87
% growth inhibition (Hom. pool) 3.39


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 7340576
Download HIP data (tab-delimited text)  (excel)
Gene:GCD6(YDR211W)|FD-Score:-3.4|P-value:3.33E-4|Clearance:0||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:GLC7(YER133W)|FD-Score:-3.24|P-value:6.00E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GLN4(YOR168W)|FD-Score:-3.47|P-value:2.59E-4|Clearance:0||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:MIA40(YKL195W)|FD-Score:3.31|P-value:4.60E-4|Clearance:0.22||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NMT1(YLR195C)|FD-Score:-3.35|P-value:4.07E-4|Clearance:0||SGD DESC:N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction Gene:RPG1(YBR079C)|FD-Score:3.6|P-value:1.60E-4|Clearance:0.05||SGD DESC:eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation Gene:RPT5(YOR117W)|FD-Score:4.39|P-value:5.78E-6|Clearance:0.79||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:SPC105(YGL093W)|FD-Score:3.55|P-value:1.91E-4|Clearance:0.15||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:TBF1(YPL128C)|FD-Score:-4.69|P-value:1.35E-6|Clearance:0||SGD DESC:Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation Gene:TCP1(YDR212W)|FD-Score:6.77|P-value:6.41E-12|Clearance:2.38||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TOM22(YNL131W)|FD-Score:3.38|P-value:3.67E-4|Clearance:0.06||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:YDR187C(YDR187C_d)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:GCD6(YDR211W)|FD-Score:-3.4|P-value:3.33E-4|Clearance:0||SGD DESC:Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress Gene:GLC7(YER133W)|FD-Score:-3.24|P-value:6.00E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GLN4(YOR168W)|FD-Score:-3.47|P-value:2.59E-4|Clearance:0||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:MIA40(YKL195W)|FD-Score:3.31|P-value:4.60E-4|Clearance:0.22||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NMT1(YLR195C)|FD-Score:-3.35|P-value:4.07E-4|Clearance:0||SGD DESC:N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction Gene:RPG1(YBR079C)|FD-Score:3.6|P-value:1.60E-4|Clearance:0.05||SGD DESC:eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation Gene:RPT5(YOR117W)|FD-Score:4.39|P-value:5.78E-6|Clearance:0.79||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:SPC105(YGL093W)|FD-Score:3.55|P-value:1.91E-4|Clearance:0.15||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:TBF1(YPL128C)|FD-Score:-4.69|P-value:1.35E-6|Clearance:0||SGD DESC:Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation Gene:TCP1(YDR212W)|FD-Score:6.77|P-value:6.41E-12|Clearance:2.38||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TOM22(YNL131W)|FD-Score:3.38|P-value:3.67E-4|Clearance:0.06||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:YDR187C(YDR187C_d)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 7340576
Download HOP data (tab-delimited text)  (excel)
Gene:ADE4(YMR300C)|FD-Score:4.33|P-value:7.37E-6||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:ADE8(YDR408C)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AMS1(YGL156W)|FD-Score:3.79|P-value:7.65E-5||SGD DESC:Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway Gene:APL2(YKL135C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:ATG12(YBR217W)|FD-Score:-3.14|P-value:8.39E-4||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:ATP23(YNR020C)|FD-Score:4.53|P-value:3.01E-6||SGD DESC:Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p Gene:BER1(YLR412W)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BIO4(YNR057C)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BSP1(YPR171W)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:CIN5(YOR028C)|FD-Score:-3.86|P-value:5.62E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication Gene:CKB1(YGL019W)|FD-Score:3.73|P-value:9.47E-5||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CLD1(YGR110W)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:COX7(YMR256C)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CPS1(YJL172W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions Gene:CYC2(YOR037W)|FD-Score:-4.09|P-value:2.13E-5||SGD DESC:Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p) Gene:DCC1(YCL016C)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DPB4(YDR121W)|FD-Score:5.07|P-value:1.96E-7||SGD DESC:Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization Gene:ELP2(YGR200C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:GAP1(YKR039W)|FD-Score:6.6|P-value:2.00E-11||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GDH3(YAL062W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GPH1(YPR160W)|FD-Score:-6.09|P-value:5.80E-10||SGD DESC:Non-essential glycogen phosphorylase required for the mobilization of glycogen, activity is regulated by cyclic AMP-mediated phosphorylation, expression is regulated by stress-response elements and by the HOG MAP kinase pathway Gene:KRE1(YNL322C)|FD-Score:5.78|P-value:3.74E-9||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:KSS1(YGR040W)|FD-Score:9.28|P-value:8.74E-21||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:MCH2(YKL221W)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MND2(YIR025W)|FD-Score:5.62|P-value:9.41E-9||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MNN5(YJL186W)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MPC54(YOR177C)|FD-Score:-4.84|P-value:6.44E-7||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MSD1(YPL104W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MSN1(YOL116W)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MVP1(YMR004W)|FD-Score:7.5|P-value:3.28E-14||SGD DESC:Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress Gene:NBP2(YDR162C)|FD-Score:-8.43|P-value:1.75E-17||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NRG2(YBR066C)|FD-Score:-3.27|P-value:5.39E-4||SGD DESC:Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication Gene:OXP1(YKL215C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PAR32(YDL173W)|FD-Score:-3.33|P-value:4.38E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PCL9(YDL179W)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:QDR1(YIL120W)|FD-Score:-4.43|P-value:4.67E-6||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication Gene:RAD55(YDR076W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:REH1(YLR387C)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RMD9(YGL107C)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RPN14(YGL004C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p Gene:RSC2(YLR357W)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:RTA1(YGR213C)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein Gene:SCS3(YGL126W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol Gene:SLZ1(YNL196C_p)|FD-Score:3.83|P-value:6.45E-5||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SPS2(YDR522C)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SPT10(YJL127C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SWM2(YNR004W_p)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:TMS1(YDR105C)|FD-Score:3.79|P-value:7.50E-5||SGD DESC:Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance Gene:TNA1(YGR260W)|FD-Score:3.9|P-value:4.79E-5||SGD DESC:High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) Gene:TRM10(YOL093W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:TRM3(YDL112W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:VPS17(YOR132W)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:VPS74(YDR372C)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:XRS2(YDR369C)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBR300C(YBR300C_d)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YBT1(YLL048C)|FD-Score:8.43|P-value:1.78E-17||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YDR179W-A(YDR179W-A_p)|FD-Score:5.47|P-value:2.28E-8||SGD DESC:Putative protein of unknown function Gene:YDR541C(YDR541C_p)|FD-Score:3.78|P-value:7.87E-5||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YET2(YMR040W)|FD-Score:3.95|P-value:3.88E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YGR012W(YGR012W)|FD-Score:5.04|P-value:2.35E-7||SGD DESC:Putative cysteine synthase, localized to the mitochondrial outer membrane Gene:YGR066C(YGR066C_p)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Putative protein of unknown function Gene:YHR138C(YHR138C_p)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress Gene:YIL086C(YIL086C_d)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIM1(YMR152W)|FD-Score:-4.89|P-value:5.03E-7||SGD DESC:Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:YJR061W(YJR061W_p)|FD-Score:3.86|P-value:5.73E-5||SGD DESC:Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication Gene:YKL075C(YKL075C_p)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YMR031W-A(YMR031W-A_d)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR099C(YMR099C)|FD-Score:4.5|P-value:3.43E-6||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YMR175W-A(YMR175W-A_p)|FD-Score:-4.06|P-value:2.49E-5||SGD DESC:Putative protein of unknown function Gene:YMR209C(YMR209C_p)|FD-Score:6.13|P-value:4.39E-10||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YNL170W(YNL170W_d)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR283W(YOR283W)|FD-Score:5.93|P-value:1.49E-9||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YPR092W(YPR092W_d)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ADE4(YMR300C)|FD-Score:4.33|P-value:7.37E-6||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:ADE8(YDR408C)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AMS1(YGL156W)|FD-Score:3.79|P-value:7.65E-5||SGD DESC:Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway Gene:APL2(YKL135C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:ATG12(YBR217W)|FD-Score:-3.14|P-value:8.39E-4||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:ATP23(YNR020C)|FD-Score:4.53|P-value:3.01E-6||SGD DESC:Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p Gene:BER1(YLR412W)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BIO4(YNR057C)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BSP1(YPR171W)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:CIN5(YOR028C)|FD-Score:-3.86|P-value:5.62E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication Gene:CKB1(YGL019W)|FD-Score:3.73|P-value:9.47E-5||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CLD1(YGR110W)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:COX7(YMR256C)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CPS1(YJL172W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions Gene:CYC2(YOR037W)|FD-Score:-4.09|P-value:2.13E-5||SGD DESC:Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p) Gene:DCC1(YCL016C)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DPB4(YDR121W)|FD-Score:5.07|P-value:1.96E-7||SGD DESC:Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization Gene:ELP2(YGR200C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:GAP1(YKR039W)|FD-Score:6.6|P-value:2.00E-11||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GDH3(YAL062W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GPH1(YPR160W)|FD-Score:-6.09|P-value:5.80E-10||SGD DESC:Non-essential glycogen phosphorylase required for the mobilization of glycogen, activity is regulated by cyclic AMP-mediated phosphorylation, expression is regulated by stress-response elements and by the HOG MAP kinase pathway Gene:KRE1(YNL322C)|FD-Score:5.78|P-value:3.74E-9||SGD DESC:Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor Gene:KSS1(YGR040W)|FD-Score:9.28|P-value:8.74E-21||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:MCH2(YKL221W)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MND2(YIR025W)|FD-Score:5.62|P-value:9.41E-9||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MNN5(YJL186W)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MPC54(YOR177C)|FD-Score:-4.84|P-value:6.44E-7||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MSD1(YPL104W)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MSN1(YOL116W)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:MVP1(YMR004W)|FD-Score:7.5|P-value:3.28E-14||SGD DESC:Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress Gene:NBP2(YDR162C)|FD-Score:-8.43|P-value:1.75E-17||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NRG2(YBR066C)|FD-Score:-3.27|P-value:5.39E-4||SGD DESC:Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication Gene:OXP1(YKL215C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PAR32(YDL173W)|FD-Score:-3.33|P-value:4.38E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PCL9(YDL179W)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:QDR1(YIL120W)|FD-Score:-4.43|P-value:4.67E-6||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication Gene:RAD55(YDR076W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:REH1(YLR387C)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RMD9(YGL107C)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RPN14(YGL004C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p Gene:RSC2(YLR357W)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:RTA1(YGR213C)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein Gene:SCS3(YGL126W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Protein required for inositol prototrophy; required for normal ER membrane biosynthesis; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; disputed role in the synthesis of inositol phospholipids from inositol Gene:SLZ1(YNL196C_p)|FD-Score:3.83|P-value:6.45E-5||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SPS2(YDR522C)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SPT10(YJL127C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SWM2(YNR004W_p)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:TMS1(YDR105C)|FD-Score:3.79|P-value:7.50E-5||SGD DESC:Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance Gene:TNA1(YGR260W)|FD-Score:3.9|P-value:4.79E-5||SGD DESC:High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) Gene:TRM10(YOL093W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:TRM3(YDL112W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:VPS17(YOR132W)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:VPS74(YDR372C)|FD-Score:3.97|P-value:3.54E-5||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:XRS2(YDR369C)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBR300C(YBR300C_d)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YBT1(YLL048C)|FD-Score:8.43|P-value:1.78E-17||SGD DESC:Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters Gene:YDR179W-A(YDR179W-A_p)|FD-Score:5.47|P-value:2.28E-8||SGD DESC:Putative protein of unknown function Gene:YDR541C(YDR541C_p)|FD-Score:3.78|P-value:7.87E-5||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YET2(YMR040W)|FD-Score:3.95|P-value:3.88E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YGR012W(YGR012W)|FD-Score:5.04|P-value:2.35E-7||SGD DESC:Putative cysteine synthase, localized to the mitochondrial outer membrane Gene:YGR066C(YGR066C_p)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Putative protein of unknown function Gene:YHR138C(YHR138C_p)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress Gene:YIL086C(YIL086C_d)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIM1(YMR152W)|FD-Score:-4.89|P-value:5.03E-7||SGD DESC:Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:YJR061W(YJR061W_p)|FD-Score:3.86|P-value:5.73E-5||SGD DESC:Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication Gene:YKL075C(YKL075C_p)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:YMR031W-A(YMR031W-A_d)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR099C(YMR099C)|FD-Score:4.5|P-value:3.43E-6||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YMR175W-A(YMR175W-A_p)|FD-Score:-4.06|P-value:2.49E-5||SGD DESC:Putative protein of unknown function Gene:YMR209C(YMR209C_p)|FD-Score:6.13|P-value:4.39E-10||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YNL170W(YNL170W_d)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR283W(YOR283W)|FD-Score:5.93|P-value:1.49E-9||SGD DESC:Phosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene Gene:YPR092W(YPR092W_d)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YDR212W6.776.41E-122.38TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YOR117W4.395.78E-60.79RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YBR079C3.601.60E-40.05RPG1eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation
YGL093W3.551.91E-40.15SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YDR187C_d3.403.33E-40.03YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YNL131W3.383.67E-40.06TOM22Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes
YKL195W3.314.60E-40.23MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YJL086C_d3.090.001000.05YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1
YBR142W3.040.001190.33MAK5Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
YPR085C2.710.003340.01ASA1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
YML091C2.700.003440.04RPM2Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus
YKL196C2.660.003910.15YKT6Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus
YMR203W2.510.005970.09TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YLR153C2.420.007760.02ACS2Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
YKL182W2.400.008170.06FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities

Top 15 HOM fitness defect scores (HOP)

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Deletion Strain FD score P-value Gene Gene Description
YGR040W9.288.74E-21KSS1Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains
YLL048C8.431.78E-17YBT1Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters
YMR004W7.503.28E-14MVP1Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress
YKR039W6.602.00E-11GAP1General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth
YMR209C_p6.134.39E-10YMR209C_pPutative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene
YOR283W5.931.49E-9YOR283WPhosphatase with a broad substrate specificity and some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene
YNL322C5.783.74E-9KRE1Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
YIR025W5.629.41E-9MND2Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase
YDR179W-A_p5.472.28E-8YDR179W-A_pPutative protein of unknown function
YDR121W5.071.96E-7DPB4Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
YGR012W5.042.35E-7YGR012WPutative cysteine synthase, localized to the mitochondrial outer membrane
YNR020C4.533.01E-6ATP23Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p
YMR099C4.503.43E-6YMR099CGlucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
YMR300C4.337.37E-6ADE4Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway
YKL215C4.261.02E-5OXP15-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_962
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1018.09E-15SGTC_245methotrexate 289.6 μMMiscellaneous1269410.0927835
0.0741.35E-8SGTC_2779methotrexate 400.0 μMMiscellaneous1269410.0927835
0.0648.86E-7SGTC_28729044037 26.0 μMChembridge (Drug-like library)64651950.2
0.0612.93E-6SGTC_12044049-0315 18.3 μMChemDiv (Drug-like library)5884020.0985916
0.0571.33E-5SGTC_9571187-1561 14.1 μMChemDiv (Drug-like library)15384540.0441176
0.0543.09E-5SGTC_6470140-0236 81.0 μMChemDiv (Drug-like library)9908370.0769231
0.0543.28E-5SGTC_32449134489 49.5 μMChembridge (Drug-like library)165842710.117647
0.0543.39E-5SGTC_9631269-2853 151.0 μMChemDiv (Drug-like library)36682230.0958904
0.0518.05E-5SGTC_7211187-1332 279.0 μMChemDiv (Drug-like library)257300.166667cell wall signaling
0.0518.33E-5SGTC_698k064-0052 127.0 μMChemDiv (Drug-like library)50887510.0823529DNA damage response
0.0511.00E-4SGTC_1062mesoridazine 14.2 μMMiscellaneous40780.0777778
0.0501.06E-4SGTC_460trequinsin 123.0 μMMiscellaneous55370.091954
0.0501.37E-4SGTC_2762leflunomide 42.1 μMMiscellaneous38990.121622
0.0491.73E-4SGTC_9531188-0045 9.7 μMChemDiv (Drug-like library)61444260.069444460S ribosome export
0.0482.22E-4SGTC_1816st051957 91.6 μMTimTec (Natural product derivative library)6741210.134328

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_6681682-049962.6 μM0.433333682214ChemDiv (Drug-like library)324.20184.10612
SGTC_12001643-019318.4 μM0.3561645403974ChemDiv (Drug-like library)347.753063.56315
SGTC_22436634756200 μM0.344828885676Chembridge (Fragment library)234.079262.01512
SGTC_1884563344425 μM0.3181825724239Miscellaneous287.744162.64113redox potentiating
SGTC_2485563344417.78 μM0.3181825724239Miscellaneous287.744162.64113redox potentiating
SGTC_7950685-026578 μM0.311475302754ChemDiv (Drug-like library)340.599643.86212excess fatty acid
SGTC_7911348-1292225 μM0.2987015437054ChemDiv (Drug-like library)465.294543.2917
SGTC_9511171-057982.7 μM0.2985071616050ChemDiv (Drug-like library)445.059542.71215cell wall
SGTC_1300136-0219337.66 μM0.290323234244ChemDiv (Drug-like library)296.148643.77812
SGTC_2826799254119.48 μM0.2857142985066Chembridge (Drug-like library)297.6845063.52514
SGTC_1700st0308407.93 μM0.2839515499307TimTec (Natural product derivative library)359.333382.92417PDR1