1306-0166

N-(2,4-dimethylphenyl)-5-methyl-4-nitro-1H-pyrazol-3-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_967
Screen concentration 352.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 4437607
SMILES CC1=CC(=C(C=C1)NC2=NNC(=C2[N+](=O)[O-])C)C
Standardized SMILES Cc1ccc(Nc2[nH]nc(C)c2[N+](=O)[O-])c(C)c1
Molecular weight 246.2652
ALogP 3.38
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.98
% growth inhibition (Hom. pool) 9.39


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4437607
Download HIP data (tab-delimited text)  (excel)
Gene:APC5(YOR249C)|FD-Score:-3.19|P-value:6.99E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HEM4(YOR278W)|FD-Score:-4.06|P-value:2.49E-5|Clearance:0||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:MED8(YBR193C)|FD-Score:-3.42|P-value:3.16E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NAR1(YNL240C)|FD-Score:3.27|P-value:5.39E-4|Clearance:0.14||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:OST2(YOR103C)|FD-Score:3.27|P-value:5.33E-4|Clearance:0.14||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:RPA135(YPR010C)|FD-Score:3.43|P-value:3.01E-4|Clearance:0.14||SGD DESC:RNA polymerase I second largest subunit A135 Gene:RPB10(YOR210W)|FD-Score:3.38|P-value:3.56E-4|Clearance:0.14||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:SRP21(YKL122C)|FD-Score:3.13|P-value:8.82E-4|Clearance:0.04||SGD DESC:Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm Gene:APC5(YOR249C)|FD-Score:-3.19|P-value:6.99E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HEM4(YOR278W)|FD-Score:-4.06|P-value:2.49E-5|Clearance:0||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:MED8(YBR193C)|FD-Score:-3.42|P-value:3.16E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NAR1(YNL240C)|FD-Score:3.27|P-value:5.39E-4|Clearance:0.14||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:OST2(YOR103C)|FD-Score:3.27|P-value:5.33E-4|Clearance:0.14||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:RPA135(YPR010C)|FD-Score:3.43|P-value:3.01E-4|Clearance:0.14||SGD DESC:RNA polymerase I second largest subunit A135 Gene:RPB10(YOR210W)|FD-Score:3.38|P-value:3.56E-4|Clearance:0.14||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:SRP21(YKL122C)|FD-Score:3.13|P-value:8.82E-4|Clearance:0.04||SGD DESC:Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4437607
Download HOP data (tab-delimited text)  (excel)
Gene:ADE8(YDR408C)|FD-Score:-5.35|P-value:4.33E-8||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AFT1(YGL071W)|FD-Score:-3.73|P-value:9.69E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:API2(YDR525W_d)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:ARE1(YCR048W)|FD-Score:3.8|P-value:7.33E-5||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication Gene:ARE2(YNR019W)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication Gene:ATP3(YBR039W)|FD-Score:4.35|P-value:6.95E-6||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BEM4(YPL161C)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BIT2(YBR270C_p)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:CHO1(YER026C)|FD-Score:5.06|P-value:2.10E-7||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:COX6(YHR051W)|FD-Score:5.02|P-value:2.54E-7||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CTA1(YDR256C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:DAS1(YJL149W)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DIG1(YPL049C)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DSK2(YMR276W)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:EFT1(YOR133W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:ERJ5(YFR041C)|FD-Score:3.82|P-value:6.60E-5||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:FIN1(YDR130C)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:FUN14(YAL008W)|FD-Score:5.57|P-value:1.26E-8||SGD DESC:Mitochondrial protein of unknown function Gene:FYV10(YIL097W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GAP1(YKR039W)|FD-Score:7.63|P-value:1.19E-14||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GCN5(YGR252W)|FD-Score:3.95|P-value:3.95E-5||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:HHF1(YBR009C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HIR3(YJR140C)|FD-Score:4.01|P-value:2.98E-5||SGD DESC:Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein Gene:HMI1(YOL095C)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HPT1(YDR399W)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome Gene:IMP1(YMR150C)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:IRC3(YDR332W)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:KAR5(YMR065W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:KEL1(YHR158C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:KEL2(YGR238C)|FD-Score:3.86|P-value:5.62E-5||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LTV1(YKL143W)|FD-Score:3.85|P-value:5.98E-5||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MAK3(YPR051W)|FD-Score:5.33|P-value:4.99E-8||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MCH5(YOR306C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MEF2(YJL102W)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MHF2(YDL160C-A)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 Gene:MHR1(YDR296W)|FD-Score:3.93|P-value:4.25E-5||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MMT2(YPL224C)|FD-Score:4.34|P-value:7.25E-6||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MRP4(YHL004W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRP8(YKL142W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis Gene:MRPS17(YMR188C)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MVP1(YMR004W)|FD-Score:-3.14|P-value:8.32E-4||SGD DESC:Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress Gene:NCR1(YPL006W)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:NMA111(YNL123W)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:OPI10(YOL032W)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress Gene:PHO3(YBR092C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin Gene:PIN3(YPR154W)|FD-Score:4.91|P-value:4.54E-7||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:POS5(YPL188W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:PUG1(YER185W)|FD-Score:3.78|P-value:7.97E-5||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:QRI5(YLR204W)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAD17(YOR368W)|FD-Score:5.28|P-value:6.35E-8||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RGA1(YOR127W)|FD-Score:5.15|P-value:1.28E-7||SGD DESC:GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RIM1(YCR028C-A)|FD-Score:5.6|P-value:1.10E-8||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RPS29A(YLR388W)|FD-Score:4.43|P-value:4.76E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:6.18|P-value:3.15E-10||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RSR1(YGR152C)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:SCS7(YMR272C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SEG2(YKL105C_p)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:SHE1(YBL031W)|FD-Score:4.95|P-value:3.67E-7||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SIA1(YOR137C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SIP4(YJL089W)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SKY1(YMR216C)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SPG3(YDR504C)|FD-Score:-3.81|P-value:6.97E-5||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SSD1(YDR293C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUV3(YPL029W)|FD-Score:4.33|P-value:7.57E-6||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SVP26(YHR181W)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:SWH1(YAR042W)|FD-Score:3.93|P-value:4.25E-5||SGD DESC:Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction Gene:SWS2(YNL081C)|FD-Score:4.85|P-value:6.23E-7||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TCA17(YEL048C)|FD-Score:3.87|P-value:5.42E-5||SGD DESC:Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder Gene:TCB2(YNL087W)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:TIR3(YIL011W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:TOK1(YJL093C)|FD-Score:3.85|P-value:6.01E-5||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:VPS24(YKL041W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS64(YDR200C)|FD-Score:5.76|P-value:4.09E-9||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:VPS75(YNL246W)|FD-Score:6.19|P-value:2.97E-10||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:YCR102W-A(YCR102W-A_d)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR056C(YDR056C_p)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YDR193W(YDR193W_d)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR209C(YDR209C_d)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W. Gene:YDR336W(YDR336W_p)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene Gene:YGR168C(YGR168C_p)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YGR266W(YGR266W)|FD-Score:4.27|P-value:9.61E-6||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YHR182W(YHR182W_p)|FD-Score:-3.72|P-value:9.88E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YIL002W-A(YIL002W-A_p)|FD-Score:4.33|P-value:7.42E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIP4(YGL198W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YLL007C(YLL007C_p)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR042C(YLR042C_p)|FD-Score:5.59|P-value:1.15E-8||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YLR046C(YLR046C_p)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YLR255C(YLR255C_d)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML012C-A(YML012C-A_d)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR209C(YMR209C_p)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YNL108C(YNL108C_p)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YOR304C-A(YOR304C-A_p)|FD-Score:4.37|P-value:6.24E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YPL034W(YPL034W_p)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPL247C(YPL247C_p)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YPR148C(YPR148C)|FD-Score:-3.85|P-value:5.98E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YTA7(YGR270W)|FD-Score:-4.13|P-value:1.80E-5||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:ADE8(YDR408C)|FD-Score:-5.35|P-value:4.33E-8||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AFT1(YGL071W)|FD-Score:-3.73|P-value:9.69E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:API2(YDR525W_d)|FD-Score:3.82|P-value:6.69E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:ARE1(YCR048W)|FD-Score:3.8|P-value:7.33E-5||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication Gene:ARE2(YNR019W)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication Gene:ATP3(YBR039W)|FD-Score:4.35|P-value:6.95E-6||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BEM4(YPL161C)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BIT2(YBR270C_p)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:CHO1(YER026C)|FD-Score:5.06|P-value:2.10E-7||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:COX6(YHR051W)|FD-Score:5.02|P-value:2.54E-7||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:CTA1(YDR256C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:DAS1(YJL149W)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DIG1(YPL049C)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DSK2(YMR276W)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress Gene:EFT1(YOR133W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:ERJ5(YFR041C)|FD-Score:3.82|P-value:6.60E-5||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:FIN1(YDR130C)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress Gene:FUN14(YAL008W)|FD-Score:5.57|P-value:1.26E-8||SGD DESC:Mitochondrial protein of unknown function Gene:FYV10(YIL097W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GAP1(YKR039W)|FD-Score:7.63|P-value:1.19E-14||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GCN5(YGR252W)|FD-Score:3.95|P-value:3.95E-5||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:HHF1(YBR009C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HIR3(YJR140C)|FD-Score:4.01|P-value:2.98E-5||SGD DESC:Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein Gene:HMI1(YOL095C)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HPT1(YDR399W)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome Gene:IMP1(YMR150C)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:IRC3(YDR332W)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:KAR5(YMR065W)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:KEL1(YHR158C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:KEL2(YGR238C)|FD-Score:3.86|P-value:5.62E-5||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LTV1(YKL143W)|FD-Score:3.85|P-value:5.98E-5||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MAK3(YPR051W)|FD-Score:5.33|P-value:4.99E-8||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MCH5(YOR306C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport Gene:MEF2(YJL102W)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MHF2(YDL160C-A)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 Gene:MHR1(YDR296W)|FD-Score:3.93|P-value:4.25E-5||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MMT2(YPL224C)|FD-Score:4.34|P-value:7.25E-6||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MRP4(YHL004W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRP8(YKL142W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis Gene:MRPS17(YMR188C)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MVP1(YMR004W)|FD-Score:-3.14|P-value:8.32E-4||SGD DESC:Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress Gene:NCR1(YPL006W)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:NMA111(YNL123W)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:OPI10(YOL032W)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress Gene:PHO3(YBR092C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin Gene:PIN3(YPR154W)|FD-Score:4.91|P-value:4.54E-7||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:POS5(YPL188W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Mitochondrial NADH kinase, phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress Gene:PUG1(YER185W)|FD-Score:3.78|P-value:7.97E-5||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:QRI5(YLR204W)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAD17(YOR368W)|FD-Score:5.28|P-value:6.35E-8||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RGA1(YOR127W)|FD-Score:5.15|P-value:1.28E-7||SGD DESC:GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RIM1(YCR028C-A)|FD-Score:5.6|P-value:1.10E-8||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RPS29A(YLR388W)|FD-Score:4.43|P-value:4.76E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:6.18|P-value:3.15E-10||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RSR1(YGR152C)|FD-Score:3.84|P-value:6.11E-5||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:SCS7(YMR272C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SEG2(YKL105C_p)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:SHE1(YBL031W)|FD-Score:4.95|P-value:3.67E-7||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SIA1(YOR137C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SIP4(YJL089W)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SKY1(YMR216C)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SPG3(YDR504C)|FD-Score:-3.81|P-value:6.97E-5||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SSD1(YDR293C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUV3(YPL029W)|FD-Score:4.33|P-value:7.57E-6||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SVP26(YHR181W)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:SWH1(YAR042W)|FD-Score:3.93|P-value:4.25E-5||SGD DESC:Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction Gene:SWS2(YNL081C)|FD-Score:4.85|P-value:6.23E-7||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TCA17(YEL048C)|FD-Score:3.87|P-value:5.42E-5||SGD DESC:Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder Gene:TCB2(YNL087W)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:TIR3(YIL011W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:TOK1(YJL093C)|FD-Score:3.85|P-value:6.01E-5||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:VPS24(YKL041W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS64(YDR200C)|FD-Score:5.76|P-value:4.09E-9||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:VPS75(YNL246W)|FD-Score:6.19|P-value:2.97E-10||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:YCR102W-A(YCR102W-A_d)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR056C(YDR056C_p)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YDR193W(YDR193W_d)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR209C(YDR209C_d)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YDR210W. Gene:YDR336W(YDR336W_p)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene Gene:YGR168C(YGR168C_p)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YGR266W(YGR266W)|FD-Score:4.27|P-value:9.61E-6||SGD DESC:Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress Gene:YHR182W(YHR182W_p)|FD-Score:-3.72|P-value:9.88E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YIL002W-A(YIL002W-A_p)|FD-Score:4.33|P-value:7.42E-6||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIP4(YGL198W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YLL007C(YLL007C_p)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR042C(YLR042C_p)|FD-Score:5.59|P-value:1.15E-8||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YLR046C(YLR046C_p)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YLR255C(YLR255C_d)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML012C-A(YML012C-A_d)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YMR209C(YMR209C_p)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YNL108C(YNL108C_p)|FD-Score:-3.26|P-value:5.59E-4||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YOR304C-A(YOR304C-A_p)|FD-Score:4.37|P-value:6.24E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck Gene:YPL034W(YPL034W_p)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPL247C(YPL247C_p)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YPR148C(YPR148C)|FD-Score:-3.85|P-value:5.98E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YTA7(YGR270W)|FD-Score:-4.13|P-value:1.80E-5||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR010C3.433.01E-40.14RPA135RNA polymerase I second largest subunit A135
YOR210W3.383.56E-40.14RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YOR103C3.275.33E-40.14OST2Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YNL240C3.275.39E-40.14NAR1Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf
YKL122C3.138.82E-40.04SRP21Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm
YDR037W3.090.001020.06KRS1Lysyl-tRNA synthetase
YER006W3.030.001230.04NUG1GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus
YJL026W2.990.001390.11RNR2Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits
YGR251W2.880.001990.03NOP19Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes
YOR206W2.850.002180.02NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
YOL120C2.830.002350.03RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YOL146W2.800.002550.01PSF3Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YKL193C2.790.002660.13SDS22Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function
YOR207C2.660.003960.02RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YHR042W2.640.004200.01NCP1NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKR039W7.631.19E-14GAP1General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth
YNL246W6.192.97E-10VPS75NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress
YOL117W6.183.15E-10RRI2Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response
YDR200C5.764.09E-9VPS64Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance
YCR028C-A5.601.10E-8RIM1Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication
YLR042C_p5.591.15E-8YLR042C_pProtein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene
YAL008W5.571.26E-8FUN14Mitochondrial protein of unknown function
YPR051W5.334.99E-8MAK3Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus
YOR368W5.286.35E-8RAD17Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins
YOR127W5.151.28E-7RGA1GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress
YER026C5.062.10E-7CHO1Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline
YHR051W5.022.54E-7COX6Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels
YBL031W4.953.67E-7SHE1Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function
YPR154W4.914.54E-7PIN3Protein that induces appearance of [PIN+] prion when overproduced
YNL081C4.856.23E-7SWS2Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency

GO enrichment analysis for SGTC_967
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0872.51E-11SGTC_9681309-0633 340.0 μMChemDiv (Drug-like library)37619760.0909091
0.0715.31E-8SGTC_1731273-0061 2.7 μMChemDiv (Drug-like library)28474140.0597015mitochondrial stress
0.0691.13E-7SGTC_1623st003709 20.3 μMTimTec (Natural product derivative library)161926180.0978261calcium & mitochondrial duress
0.0672.80E-7SGTC_9923909-8734 162.0 μMChemDiv (Drug-like library)42883190.142857
0.0631.46E-6SGTC_9611252-1571 372.0 μMChemDiv (Drug-like library)68134980.0746269
0.0621.99E-6SGTC_9561185-0240 191.0 μMChemDiv (Drug-like library)14161930.060241
0.0612.59E-6SGTC_20625245942 200.0 μMChembridge (Fragment library)13761600.0793651
0.0595.59E-6SGTC_8720588-0139 234.0 μMChemDiv (Drug-like library)20471000.056338
0.0571.07E-5SGTC_1627st003713 25.6 μMTimTec (Natural product derivative library)161950700.0537634calcium & mitochondrial duress
0.0561.91E-5SGTC_630099-0242 26.7 μMChemDiv (Drug-like library)38985020.0677966mitochondrial stress
0.0552.12E-5SGTC_9591222-0044 107.0 μMChemDiv (Drug-like library)60836120.078125
0.0526.91E-5SGTC_100279-0212 42.6 μMChemDiv (Drug-like library)398510.0689655TRP & mitochondrial translation
0.0501.18E-4SGTC_6480375-0473 33.3 μMChemDiv (Drug-like library)17131390.0983607
0.0501.19E-4SGTC_2543pimpinellin 60.7 μMMicrosource (Natural product library)48250.0757576Golgi
0.0501.26E-4SGTC_3851494-0393 12.4 μMChemDiv (Drug-like library)30906470.0740741

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_13191306-015543.7 μM0.5652173122360ChemDiv (Drug-like library)252.657063.06724
SGTC_13591582-0056520 nM0.3448283661189ChemDiv (Drug-like library)304.688563.99416
SGTC_13311472-004976.2 μM0.31034524051409ChemDiv (Drug-like library)286.325723.57824
SGTC_3106912128649.47 μM0.29508215943471Chembridge (Drug-like library)299.41063.62812
SGTC_2840988-010432.6 μM0.2931034666532ChemDiv (Drug-like library)329.3525.28314heme requiring
SGTC_15141189-1591158 μM0.280702767698ChemDiv (Drug-like library)320.363543.6715
SGTC_8103825-7150143 μM0.27692322330411ChemDiv (Drug-like library)415.48436.021660S ribosome export
SGTC_5983937-023620.2 μM0.2711861379313ChemDiv (Drug-like library)354.80864.33515ERG2
SGTC_410478-063282.64 μM0.2592593750424ChemDiv (Drug-like library)229.234642.52724
SGTC_3051682-259520.8 μM0.254545226033ChemDiv (Drug-like library)260.311584.35114RNA processing & uracil transport