1313-0238

3-(4-nitrophenyl)-3-oxo-N-[3-(trifluoromethyl)phenyl]propanamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_969
Screen concentration 134.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 5212874
SMILES C1=CC(=CC(=C1)NC(=O)CC(=O)C2=CC=C(C=C2)[N+](=O)[O-])C(F)(F)F
Standardized SMILES [O-][N+](=O)c1ccc(cc1)C(=O)CC(=O)Nc2cccc(c2)C(F)(F)F
Molecular weight 352.2648
ALogP 3.37
H-bond donor count 1
H-bond acceptor count 7
Response signature mitochondrial processes

Pool Growth Kinetics
% growth inhibition (Het. pool) 56.15
% growth inhibition (Hom. pool) 5.44


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5212874
Download HIP data (tab-delimited text)  (excel)
Gene:APC5(YOR249C)|FD-Score:3.39|P-value:3.49E-4|Clearance:0.02||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:ARP4(YJL081C)|FD-Score:-3.57|P-value:1.78E-4|Clearance:0||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BET4(YJL031C)|FD-Score:4.37|P-value:6.08E-6|Clearance:0.04||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDC25(YLR310C)|FD-Score:-3.21|P-value:6.67E-4|Clearance:0||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CDC39(YCR093W)|FD-Score:-3.21|P-value:6.64E-4|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC48(YDL126C)|FD-Score:3.26|P-value:5.55E-4|Clearance:0.02||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:DBP2(YNL112W)|FD-Score:3.19|P-value:7.06E-4|Clearance:0.06||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DNA2(YHR164C)|FD-Score:3.79|P-value:7.55E-5|Clearance:0.08||SGD DESC:Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress Gene:DOP1(YDR141C)|FD-Score:-4.16|P-value:1.58E-5|Clearance:0||SGD DESC:Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Gene:DRS1(YLL008W)|FD-Score:3.21|P-value:6.57E-4|Clearance:0.01||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:ERG10(YPL028W)|FD-Score:6.37|P-value:9.68E-11|Clearance:0.43||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:ERG7(YHR072W)|FD-Score:4.66|P-value:1.59E-6|Clearance:0||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:FCP1(YMR277W)|FD-Score:3.35|P-value:4.02E-4|Clearance:0.03||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:FOL2(YGR267C)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.08||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:GCD1(YOR260W)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.01||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:HEM1(YDR232W)|FD-Score:4.66|P-value:1.59E-6|Clearance:0.14||SGD DESC:5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p Gene:HYP2(YEL034W)|FD-Score:5.65|P-value:7.82E-9|Clearance:0.39||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:LSM3(YLR438C-A)|FD-Score:5.94|P-value:1.42E-9|Clearance:0.22||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MAS2(YHR024C)|FD-Score:4.51|P-value:3.18E-6|Clearance:0.12||SGD DESC:Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MSL5(YLR116W)|FD-Score:-4.02|P-value:2.95E-5|Clearance:0||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:NNF1(YJR112W)|FD-Score:4.39|P-value:5.67E-6|Clearance:0.02||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NOC3(YLR002C)|FD-Score:3.37|P-value:3.73E-4|Clearance:0.02||SGD DESC:Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing Gene:NOP8(YOL144W)|FD-Score:13.1|P-value:1.10E-39|Clearance:2.95||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUG1(YER006W)|FD-Score:10.2|P-value:1.22E-24|Clearance:1.43||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:NUP192(YJL039C)|FD-Score:3.41|P-value:3.30E-4|Clearance:0.02||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:PRE1(YER012W)|FD-Score:-3.14|P-value:8.47E-4|Clearance:0||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PUP2(YGR253C)|FD-Score:-3.62|P-value:1.46E-4|Clearance:0||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:RGR1(YLR071C)|FD-Score:3.24|P-value:6.04E-4|Clearance:0.02||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation Gene:RIB2(YOL066C)|FD-Score:5.27|P-value:6.89E-8|Clearance:0.01||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:RIM2(YBR192W)|FD-Score:3.52|P-value:2.19E-4|Clearance:0.11||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RSC58(YLR033W)|FD-Score:4.99|P-value:3.01E-7|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:3.13|P-value:8.61E-4|Clearance:0.06||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SCL1(YGL011C)|FD-Score:-5.61|P-value:1.03E-8|Clearance:0||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SEC14(YMR079W)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.06||SGD DESC:Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Gene:SPN1(YPR133C)|FD-Score:5.26|P-value:7.38E-8|Clearance:0.15||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SPT6(YGR116W)|FD-Score:3.3|P-value:4.87E-4|Clearance:0.04||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:TCP1(YDR212W)|FD-Score:3.71|P-value:1.03E-4|Clearance:0.04||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TFC1(YBR123C)|FD-Score:3.2|P-value:6.91E-4|Clearance:0.01||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TIM10(YHR005C-A)|FD-Score:-3.37|P-value:3.83E-4|Clearance:0||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TIM17(YJL143W)|FD-Score:-3.6|P-value:1.60E-4|Clearance:0||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TIM22(YDL217C)|FD-Score:4.96|P-value:3.49E-7|Clearance:0.3||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:TOM40(YMR203W)|FD-Score:8.75|P-value:1.11E-18|Clearance:2.38||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TRZ1(YKR079C)|FD-Score:4.33|P-value:7.56E-6|Clearance:0.49||SGD DESC:tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2 Gene:UTP22(YGR090W)|FD-Score:5.11|P-value:1.62E-7|Clearance:0.12||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:YDL196W(YDL196W_d)|FD-Score:4.34|P-value:7.28E-6|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YDR355C(YDR355C_d)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W Gene:YFH1(YDL120W)|FD-Score:3.32|P-value:4.45E-4|Clearance:0.03||SGD DESC:Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia Gene:YGR114C(YGR114C_d)|FD-Score:3.84|P-value:6.26E-5|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YJL202C(YJL202C_d)|FD-Score:5.72|P-value:5.28E-9|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex Gene:APC5(YOR249C)|FD-Score:3.39|P-value:3.49E-4|Clearance:0.02||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:ARP4(YJL081C)|FD-Score:-3.57|P-value:1.78E-4|Clearance:0||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BET4(YJL031C)|FD-Score:4.37|P-value:6.08E-6|Clearance:0.04||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDC25(YLR310C)|FD-Score:-3.21|P-value:6.67E-4|Clearance:0||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:CDC39(YCR093W)|FD-Score:-3.21|P-value:6.64E-4|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC48(YDL126C)|FD-Score:3.26|P-value:5.55E-4|Clearance:0.02||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:DBP2(YNL112W)|FD-Score:3.19|P-value:7.06E-4|Clearance:0.06||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DNA2(YHR164C)|FD-Score:3.79|P-value:7.55E-5|Clearance:0.08||SGD DESC:Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress Gene:DOP1(YDR141C)|FD-Score:-4.16|P-value:1.58E-5|Clearance:0||SGD DESC:Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Gene:DRS1(YLL008W)|FD-Score:3.21|P-value:6.57E-4|Clearance:0.01||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:ERG10(YPL028W)|FD-Score:6.37|P-value:9.68E-11|Clearance:0.43||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:ERG7(YHR072W)|FD-Score:4.66|P-value:1.59E-6|Clearance:0||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:FCP1(YMR277W)|FD-Score:3.35|P-value:4.02E-4|Clearance:0.03||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:FOL2(YGR267C)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.08||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:GCD1(YOR260W)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.01||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:HEM1(YDR232W)|FD-Score:4.66|P-value:1.59E-6|Clearance:0.14||SGD DESC:5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p Gene:HYP2(YEL034W)|FD-Score:5.65|P-value:7.82E-9|Clearance:0.39||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:LSM3(YLR438C-A)|FD-Score:5.94|P-value:1.42E-9|Clearance:0.22||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MAS2(YHR024C)|FD-Score:4.51|P-value:3.18E-6|Clearance:0.12||SGD DESC:Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MSL5(YLR116W)|FD-Score:-4.02|P-value:2.95E-5|Clearance:0||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:NNF1(YJR112W)|FD-Score:4.39|P-value:5.67E-6|Clearance:0.02||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NOC3(YLR002C)|FD-Score:3.37|P-value:3.73E-4|Clearance:0.02||SGD DESC:Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing Gene:NOP8(YOL144W)|FD-Score:13.1|P-value:1.10E-39|Clearance:2.95||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NUG1(YER006W)|FD-Score:10.2|P-value:1.22E-24|Clearance:1.43||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:NUP192(YJL039C)|FD-Score:3.41|P-value:3.30E-4|Clearance:0.02||SGD DESC:Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 Gene:PRE1(YER012W)|FD-Score:-3.14|P-value:8.47E-4|Clearance:0||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PUP2(YGR253C)|FD-Score:-3.62|P-value:1.46E-4|Clearance:0||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:RGR1(YLR071C)|FD-Score:3.24|P-value:6.04E-4|Clearance:0.02||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation Gene:RIB2(YOL066C)|FD-Score:5.27|P-value:6.89E-8|Clearance:0.01||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:RIM2(YBR192W)|FD-Score:3.52|P-value:2.19E-4|Clearance:0.11||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RSC58(YLR033W)|FD-Score:4.99|P-value:3.01E-7|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:3.13|P-value:8.61E-4|Clearance:0.06||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SCL1(YGL011C)|FD-Score:-5.61|P-value:1.03E-8|Clearance:0||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SEC14(YMR079W)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.06||SGD DESC:Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs Gene:SPN1(YPR133C)|FD-Score:5.26|P-value:7.38E-8|Clearance:0.15||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SPT6(YGR116W)|FD-Score:3.3|P-value:4.87E-4|Clearance:0.04||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:TCP1(YDR212W)|FD-Score:3.71|P-value:1.03E-4|Clearance:0.04||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TFC1(YBR123C)|FD-Score:3.2|P-value:6.91E-4|Clearance:0.01||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TIM10(YHR005C-A)|FD-Score:-3.37|P-value:3.83E-4|Clearance:0||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TIM17(YJL143W)|FD-Score:-3.6|P-value:1.60E-4|Clearance:0||SGD DESC:Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex Gene:TIM22(YDL217C)|FD-Score:4.96|P-value:3.49E-7|Clearance:0.3||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:TOM40(YMR203W)|FD-Score:8.75|P-value:1.11E-18|Clearance:2.38||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TRZ1(YKR079C)|FD-Score:4.33|P-value:7.56E-6|Clearance:0.49||SGD DESC:tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2 Gene:UTP22(YGR090W)|FD-Score:5.11|P-value:1.62E-7|Clearance:0.12||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:YDL196W(YDL196W_d)|FD-Score:4.34|P-value:7.28E-6|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YDR355C(YDR355C_d)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:Dubious: Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W Gene:YFH1(YDL120W)|FD-Score:3.32|P-value:4.45E-4|Clearance:0.03||SGD DESC:Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia Gene:YGR114C(YGR114C_d)|FD-Score:3.84|P-value:6.26E-5|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YJL202C(YJL202C_d)|FD-Score:5.72|P-value:5.28E-9|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5212874
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AEP1(YMR064W)|FD-Score:8.65|P-value:2.50E-18||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:ALD4(YOR374W)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed Gene:APN1(YKL114C)|FD-Score:6.6|P-value:2.12E-11||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARC1(YGL105W)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:BCK1(YJL095W)|FD-Score:-3.88|P-value:5.27E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CKB2(YOR039W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CNM67(YNL225C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:CTK1(YKL139W)|FD-Score:6.96|P-value:1.69E-12||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DDI1(YER143W)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains Gene:DPH1(YIL103W)|FD-Score:3.83|P-value:6.46E-5||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:DSS1(YMR287C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:ECM3(YOR092W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication Gene:ECM33(YBR078W)|FD-Score:6.12|P-value:4.75E-10||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ERG4(YGL012W)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:GAC1(YOR178C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock Gene:GCN5(YGR252W)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GTF1(YGR102C)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria Gene:HAL9(YOL089C)|FD-Score:3.91|P-value:4.56E-5||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HBT1(YDL223C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HHF1(YBR009C)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HTD2(YHR067W)|FD-Score:4.47|P-value:3.85E-6||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:HXT5(YHR096C)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Hexose transporter with moderate affinity for glucose; induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs; HXT5 has a paralog, HXT3, that arose from the whole genome duplication Gene:INP51(YIL002C)|FD-Score:5.03|P-value:2.40E-7||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:IRC21(YMR073C)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRC3(YDR332W)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:ISU1(YPL135W)|FD-Score:-3.29|P-value:5.06E-4||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:LAS21(YJL062W)|FD-Score:4.05|P-value:2.53E-5||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LEO1(YOR123C)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LIP2(YLR239C)|FD-Score:4.36|P-value:6.57E-6||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MAC1(YMR021C)|FD-Score:3.85|P-value:6.02E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAM33(YIL070C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MCT1(YOR221C)|FD-Score:5.92|P-value:1.57E-9||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MEF2(YJL102W)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MNT4(YNR059W)|FD-Score:4.27|P-value:9.58E-6||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MRP21(YBL090W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL8(YJL063C)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MVB12(YGR206W)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:NPL3(YDR432W)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:NST1(YNL091W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:NTA1(YJR062C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation Gene:OAR1(YKL055C)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:PET54(YGR222W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PEX32(YBR168W)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PIB1(YDR313C)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PIF1(YML061C)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PMD1(YER132C)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:PMP3(YDR276C)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PRO2(YOR323C)|FD-Score:5.63|P-value:8.90E-9||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTC6(YCR079W)|FD-Score:3.93|P-value:4.21E-5||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:PTM1(YKL039W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication Gene:RAD51(YER095W)|FD-Score:4.3|P-value:8.45E-6||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RGP1(YDR137W)|FD-Score:3.86|P-value:5.72E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RKM1(YPL208W)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp) Gene:RLF2(YPR018W)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:ROG3(YFR022W)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication Gene:RPS19A(YOL121C)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RTC3(YHR087W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:SFB3(YHR098C)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:STM1(YLR150W)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:SWA2(YDR320C)|FD-Score:4.27|P-value:9.57E-6||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TIF4631(YGR162W)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TPM1(YNL079C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:URM1(YIL008W)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VPS29(YHR012W)|FD-Score:-4.38|P-value:5.92E-6||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:YAL037W(YAL037W_p)|FD-Score:5.87|P-value:2.14E-9||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YBR296C-A(YBR296C-A_p)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR115W(YDR115W_p)|FD-Score:3.41|P-value:3.31E-4||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YHR182W(YHR182W_p)|FD-Score:-3.51|P-value:2.20E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YLR342W-A(YLR342W-A_p)|FD-Score:-5.17|P-value:1.19E-7||SGD DESC:Putative protein of unknown function Gene:YME2(YMR302C)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YMR114C(YMR114C)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene Gene:YNL184C(YNL184C_p)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL285W(YNL285W_d)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR066C(YNR066C_p)|FD-Score:5.88|P-value:2.02E-9||SGD DESC:Putative membrane-localized protein of unknown function Gene:YOR376W(YOR376W_d)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene. Gene:YSP2(YDR326C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:ACB1(YGR037C)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AEP1(YMR064W)|FD-Score:8.65|P-value:2.50E-18||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:ALD4(YOR374W)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed Gene:APN1(YKL114C)|FD-Score:6.6|P-value:2.12E-11||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARC1(YGL105W)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:BCK1(YJL095W)|FD-Score:-3.88|P-value:5.27E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CKB2(YOR039W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CNM67(YNL225C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:CTK1(YKL139W)|FD-Score:6.96|P-value:1.69E-12||SGD DESC:Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13 Gene:DDI1(YER143W)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains Gene:DPH1(YIL103W)|FD-Score:3.83|P-value:6.46E-5||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:DSS1(YMR287C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:ECM3(YOR092W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication Gene:ECM33(YBR078W)|FD-Score:6.12|P-value:4.75E-10||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ERG4(YGL012W)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:GAC1(YOR178C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock Gene:GCN5(YGR252W)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GTF1(YGR102C)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria Gene:HAL9(YOL089C)|FD-Score:3.91|P-value:4.56E-5||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HBT1(YDL223C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HHF1(YBR009C)|FD-Score:-3.41|P-value:3.19E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HTD2(YHR067W)|FD-Score:4.47|P-value:3.85E-6||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:HXT5(YHR096C)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Hexose transporter with moderate affinity for glucose; induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs; HXT5 has a paralog, HXT3, that arose from the whole genome duplication Gene:INP51(YIL002C)|FD-Score:5.03|P-value:2.40E-7||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:IRC21(YMR073C)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRC3(YDR332W)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:ISU1(YPL135W)|FD-Score:-3.29|P-value:5.06E-4||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:LAS21(YJL062W)|FD-Score:4.05|P-value:2.53E-5||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LEO1(YOR123C)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:LIP2(YLR239C)|FD-Score:4.36|P-value:6.57E-6||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MAC1(YMR021C)|FD-Score:3.85|P-value:6.02E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAM33(YIL070C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MCT1(YOR221C)|FD-Score:5.92|P-value:1.57E-9||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MEF2(YJL102W)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MNT4(YNR059W)|FD-Score:4.27|P-value:9.58E-6||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MRP21(YBL090W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL8(YJL063C)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MVB12(YGR206W)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:NPL3(YDR432W)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:NST1(YNL091W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:NTA1(YJR062C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation Gene:OAR1(YKL055C)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:PET54(YGR222W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PEX32(YBR168W)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PIB1(YDR313C)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain Gene:PIF1(YML061C)|FD-Score:4.73|P-value:1.11E-6||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PMD1(YER132C)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:PMP3(YDR276C)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PRO2(YOR323C)|FD-Score:5.63|P-value:8.90E-9||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTC6(YCR079W)|FD-Score:3.93|P-value:4.21E-5||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:PTM1(YKL039W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication Gene:RAD51(YER095W)|FD-Score:4.3|P-value:8.45E-6||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RGP1(YDR137W)|FD-Score:3.86|P-value:5.72E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RKM1(YPL208W)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp) Gene:RLF2(YPR018W)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:ROG3(YFR022W)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication Gene:RPS19A(YOL121C)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication Gene:RTC3(YHR087W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:SFB3(YHR098C)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:STM1(YLR150W)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:SWA2(YDR320C)|FD-Score:4.27|P-value:9.57E-6||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TIF4631(YGR162W)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TPM1(YNL079C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:URM1(YIL008W)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VPS29(YHR012W)|FD-Score:-4.38|P-value:5.92E-6||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:YAL037W(YAL037W_p)|FD-Score:5.87|P-value:2.14E-9||SGD DESC:Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication Gene:YBR296C-A(YBR296C-A_p)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR115W(YDR115W_p)|FD-Score:3.41|P-value:3.31E-4||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YHR182W(YHR182W_p)|FD-Score:-3.51|P-value:2.20E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YLR342W-A(YLR342W-A_p)|FD-Score:-5.17|P-value:1.19E-7||SGD DESC:Putative protein of unknown function Gene:YME2(YMR302C)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YMR114C(YMR114C)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene Gene:YNL184C(YNL184C_p)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL285W(YNL285W_d)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR066C(YNR066C_p)|FD-Score:5.88|P-value:2.02E-9||SGD DESC:Putative membrane-localized protein of unknown function Gene:YOR376W(YOR376W_d)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene. Gene:YSP2(YDR326C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOL144W13.101.10E-392.95NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YER006W10.201.22E-241.43NUG1GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus
YMR203W8.751.11E-182.38TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YPL028W6.379.68E-110.43ERG10Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis
YLR438C-A5.941.42E-90.22LSM3Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YJL202C_d5.725.28E-90.07YJL202C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex
YEL034W5.657.82E-90.39HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YOL066C5.276.89E-80.01RIB2Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs
YPR133C5.267.38E-80.15SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YGR090W5.111.62E-70.12UTP22Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals
YLR033W4.993.01E-70.03RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YDL217C4.963.49E-70.30TIM22Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported
YHR072W4.661.59E-69.68E-4ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YDR232W4.661.59E-60.14HEM15-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p
YHR024C4.513.18E-60.12MAS2Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR064W8.652.50E-18AEP1Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase
YKL139W6.961.69E-12CTK1Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
YKL114C6.602.12E-11APN1Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine
YBR078W6.124.75E-10ECM33GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p
YOR221C5.921.57E-9MCT1Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling
YNR066C_p5.882.02E-9YNR066C_pPutative membrane-localized protein of unknown function
YAL037W_p5.872.14E-9YAL037W_pPutative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication
YOR323C5.638.90E-9PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YMR073C5.191.06E-7IRC21Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study
YIL002C5.032.40E-7INP51Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth
YML061C4.731.11E-6PIF1DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells
YKL055C4.671.51E-6OAR1Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p
YHR067W4.473.85E-6HTD2Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology
YLR239C4.366.57E-6LIP2Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
YER095W4.308.45E-6RAD51Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein

GO enrichment analysis for SGTC_969
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1865.37E-47SGTC_8510394-0702 453.0 μMChemDiv (Drug-like library)7626460.169231mitochondrial processes
0.1672.52E-38SGTC_7450469-0705 296.0 μMChemDiv (Drug-like library)34867360.227273mitochondrial processes
0.1541.18E-32SGTC_21836420625 130.5 μMChembridge (Fragment library)28976190.301587mitochondrial processes
0.1541.18E-32SGTC_20665222451 176.0 μMChembridge (Fragment library)22811470.0833333heme biosynthesis & mitochondrial translocase
0.1484.19E-30SGTC_13712421-0008 32.7 μMChemDiv (Drug-like library)28275520.0649351mitochondrial processes
0.1474.60E-30SGTC_23369001819 198.7 μMChembridge (Fragment library)29896020.0909091heme biosynthesis & mitochondrial translocase
0.1402.78E-27SGTC_2129girgensonine 121.1 μMChembridge (Fragment library)4426380.0441176heme biosynthesis & mitochondrial translocase
0.1402.78E-27SGTC_29289011484 9.8 μMChembridge (Drug-like library)29947510.05heme biosynthesis & mitochondrial translocase
0.1399.33E-27SGTC_9083448-7491 47.7 μMChemDiv (Drug-like library)12030310.15873mitochondrial stress
0.1382.49E-26SGTC_690096-0274 206.7 μMChemDiv (Drug-like library)67413920.0684932mitochondrial processes
0.1361.22E-25SGTC_29309013230 14.9 μMChembridge (Drug-like library)29958570.0519481heme biosynthesis & mitochondrial translocase
0.1346.29E-25SGTC_1098clofazimine 15.1 μMNIH Clinical Collection27940.0689655heme biosynthesis & mitochondrial translocase
0.1317.60E-24SGTC_7950685-0265 78.0 μMChemDiv (Drug-like library)3027540.1875excess fatty acid
0.1286.65E-23SGTC_31619100388 49.5 μMChembridge (Drug-like library)252363680.131579iron homeostasis
0.1256.36E-22SGTC_1113artemisinin 1.2 μMTimTec (Natural product library)22400.0253165heme biosynthesis & mitochondrial translocase

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2902578700660.46 μM0.431034591003Chembridge (Drug-like library)309.240013.32516Golgi
SGTC_850868-0259160.06 μM0.41509483173ChemDiv (Drug-like library)256.256682.5431360S ribosome export
SGTC_1463k081-003234.1 μM0.413793977755ChemDiv (Drug-like library)345.114633.15415
SGTC_6260214-000933 μM0.3928573437874ChemDiv (Drug-like library)332.225222.29717copper-dependent oxidative stress
SGTC_520329-0193875 nM0.3859653739292ChemDiv (Drug-like library)325.575642.80814TSC3-RPN4
SGTC_990109-0120622.8 μM0.37037759414ChemDiv (Drug-like library)321.126163.25713
SGTC_1349flufenamic acid69 μM0.3684213371ChemDiv (Drug-like library)281.229913.95226
SGTC_9262268-140159.4 μM0.3650792931578ChemDiv (Drug-like library)390.388724.27926TSC3-RPN4
SGTC_9073448-698675.5 μM0.363636590582ChemDiv (Drug-like library)352.307813.35126
SGTC_9821491-051827.5 μM0.3548393091256ChemDiv (Drug-like library)416.265653.336211