0535-0701

3-(4-ethylpiperazin-1-yl)-5-methylpyridazino[3,4-b][1,4]benzoxazine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_97
Screen concentration 19.1 μM
Source ChemDiv (Drug-like library)
PubChem CID 2832013
SMILES CCN1CCN(CC1)C2=NN=C3C(=C2)N(C4=CC=CC=C4O3)C
Standardized SMILES CCN1CCN(CC1)c2cc3N(C)c4ccccc4Oc3nn2
Molecular weight 311.3815
ALogP 3.24
H-bond donor count 0
H-bond acceptor count 6
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 17.85
% growth inhibition (Hom. pool) 18.04


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2832013
Download HIP data (tab-delimited text)  (excel)
Gene:CDC12(YHR107C)|FD-Score:5.46|P-value:2.45E-8|Clearance:0.27||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:DBP2(YNL112W)|FD-Score:3.91|P-value:4.53E-5|Clearance:0.02||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:ERG11(YHR007C)|FD-Score:3.92|P-value:4.41E-5|Clearance:0.01||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:HYP2(YEL034W)|FD-Score:-3.15|P-value:8.10E-4|Clearance:0||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:KRI1(YNL308C)|FD-Score:3.78|P-value:7.94E-5|Clearance:0.13||SGD DESC:Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p Gene:LSG1(YGL099W)|FD-Score:23.9|P-value:2.12E-126|Clearance:12.51||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM3(YLR438C-A)|FD-Score:6.08|P-value:6.09E-10|Clearance:0.62||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM4(YER112W)|FD-Score:8.08|P-value:3.35E-16|Clearance:2||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MEX67(YPL169C)|FD-Score:5.19|P-value:1.07E-7|Clearance:1.27||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MPP10(YJR002W)|FD-Score:-3.2|P-value:6.78E-4|Clearance:0||SGD DESC:Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p Gene:NAF1(YNL124W)|FD-Score:3.2|P-value:6.89E-4|Clearance:0.04||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:NAT2(YGR147C)|FD-Score:3.16|P-value:7.85E-4|Clearance:0.03||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NDC1(YML031W)|FD-Score:-3.93|P-value:4.29E-5|Clearance:0||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NMD3(YHR170W)|FD-Score:10.4|P-value:1.47E-25|Clearance:2.31||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOG1(YPL093W)|FD-Score:11.4|P-value:2.60E-30|Clearance:1||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOP1(YDL014W)|FD-Score:3.13|P-value:8.61E-4|Clearance:0.02||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:NOP2(YNL061W)|FD-Score:-3.12|P-value:9.05E-4|Clearance:0||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:NSA1(YGL111W)|FD-Score:-3.81|P-value:6.98E-5|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:PBN1(YCL052C)|FD-Score:3.89|P-value:4.91E-5|Clearance:0.12||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:RPF2(YKR081C)|FD-Score:3.6|P-value:1.57E-4|Clearance:0.4||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPL32(YBL092W)|FD-Score:3.11|P-value:9.51E-4|Clearance:0.06||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPS13(YDR064W)|FD-Score:-3.47|P-value:2.62E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS2(YGL123W)|FD-Score:-5.17|P-value:1.19E-7|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS20(YHL015W)|FD-Score:-4.2|P-value:1.33E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:-3.71|P-value:1.04E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RSM10(YDR041W)|FD-Score:3.11|P-value:9.24E-4|Clearance:0.01||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins Gene:SGD1(YLR336C)|FD-Score:-3.37|P-value:3.76E-4|Clearance:0||SGD DESC:Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia Gene:SNU56(YDR240C)|FD-Score:3.64|P-value:1.34E-4|Clearance:0.04||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:SPN1(YPR133C)|FD-Score:-3.98|P-value:3.41E-5|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TAF12(YDR145W)|FD-Score:-3.88|P-value:5.16E-5|Clearance:0||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:UTP13(YLR222C)|FD-Score:-3.35|P-value:3.99E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP15(YMR093W)|FD-Score:-4.19|P-value:1.42E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP18(YJL069C)|FD-Score:-3.4|P-value:3.42E-4|Clearance:0||SGD DESC:Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data Gene:UTP4(YDR324C)|FD-Score:-3.88|P-value:5.19E-5|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:UTP6(YDR449C)|FD-Score:-3.14|P-value:8.54E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP8(YGR128C)|FD-Score:-3.62|P-value:1.48E-4|Clearance:0||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP9(YHR196W)|FD-Score:-3.4|P-value:3.32E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YGR114C(YGR114C_d)|FD-Score:-3.27|P-value:5.34E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YJL015C(YJL015C_d)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Gene:YPR136C(YPR136C_d)|FD-Score:-3.89|P-value:4.95E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:CDC12(YHR107C)|FD-Score:5.46|P-value:2.45E-8|Clearance:0.27||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:DBP2(YNL112W)|FD-Score:3.91|P-value:4.53E-5|Clearance:0.02||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:ERG11(YHR007C)|FD-Score:3.92|P-value:4.41E-5|Clearance:0.01||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:HYP2(YEL034W)|FD-Score:-3.15|P-value:8.10E-4|Clearance:0||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:KRI1(YNL308C)|FD-Score:3.78|P-value:7.94E-5|Clearance:0.13||SGD DESC:Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p Gene:LSG1(YGL099W)|FD-Score:23.9|P-value:2.12E-126|Clearance:12.51||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM3(YLR438C-A)|FD-Score:6.08|P-value:6.09E-10|Clearance:0.62||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSM4(YER112W)|FD-Score:8.08|P-value:3.35E-16|Clearance:2||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MEX67(YPL169C)|FD-Score:5.19|P-value:1.07E-7|Clearance:1.27||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MPP10(YJR002W)|FD-Score:-3.2|P-value:6.78E-4|Clearance:0||SGD DESC:Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p Gene:NAF1(YNL124W)|FD-Score:3.2|P-value:6.89E-4|Clearance:0.04||SGD DESC:RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p Gene:NAT2(YGR147C)|FD-Score:3.16|P-value:7.85E-4|Clearance:0.03||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:NDC1(YML031W)|FD-Score:-3.93|P-value:4.29E-5|Clearance:0||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NMD3(YHR170W)|FD-Score:10.4|P-value:1.47E-25|Clearance:2.31||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOG1(YPL093W)|FD-Score:11.4|P-value:2.60E-30|Clearance:1||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOP1(YDL014W)|FD-Score:3.13|P-value:8.61E-4|Clearance:0.02||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:NOP2(YNL061W)|FD-Score:-3.12|P-value:9.05E-4|Clearance:0||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:NSA1(YGL111W)|FD-Score:-3.81|P-value:6.98E-5|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:PBN1(YCL052C)|FD-Score:3.89|P-value:4.91E-5|Clearance:0.12||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:RPF2(YKR081C)|FD-Score:3.6|P-value:1.57E-4|Clearance:0.4||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPL32(YBL092W)|FD-Score:3.11|P-value:9.51E-4|Clearance:0.06||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPS13(YDR064W)|FD-Score:-3.47|P-value:2.62E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS2(YGL123W)|FD-Score:-5.17|P-value:1.19E-7|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS20(YHL015W)|FD-Score:-4.2|P-value:1.33E-5|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:RPS3(YNL178W)|FD-Score:-3.71|P-value:1.04E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RSM10(YDR041W)|FD-Score:3.11|P-value:9.24E-4|Clearance:0.01||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins Gene:SGD1(YLR336C)|FD-Score:-3.37|P-value:3.76E-4|Clearance:0||SGD DESC:Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia Gene:SNU56(YDR240C)|FD-Score:3.64|P-value:1.34E-4|Clearance:0.04||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:SPN1(YPR133C)|FD-Score:-3.98|P-value:3.41E-5|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:TAF12(YDR145W)|FD-Score:-3.88|P-value:5.16E-5|Clearance:0||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:UTP13(YLR222C)|FD-Score:-3.35|P-value:3.99E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP15(YMR093W)|FD-Score:-4.19|P-value:1.42E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP18(YJL069C)|FD-Score:-3.4|P-value:3.42E-4|Clearance:0||SGD DESC:Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data Gene:UTP4(YDR324C)|FD-Score:-3.88|P-value:5.19E-5|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:UTP6(YDR449C)|FD-Score:-3.14|P-value:8.54E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP8(YGR128C)|FD-Score:-3.62|P-value:1.48E-4|Clearance:0||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP9(YHR196W)|FD-Score:-3.4|P-value:3.32E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YGR114C(YGR114C_d)|FD-Score:-3.27|P-value:5.34E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YJL015C(YJL015C_d)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Gene:YPR136C(YPR136C_d)|FD-Score:-3.89|P-value:4.95E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2832013
Download HOP data (tab-delimited text)  (excel)
Gene:ADE5,7(YGL234W)|FD-Score:3.75|P-value:8.75E-5||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:AEP1(YMR064W)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:ARO1(YDR127W)|FD-Score:-4.81|P-value:7.37E-7||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG16(YMR159C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BEM2(YER155C)|FD-Score:3.8|P-value:7.27E-5||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:BIO5(YNR056C)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BMH1(YER177W)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BRE5(YNR051C)|FD-Score:5.73|P-value:5.07E-9||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:BTS1(YPL069C)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD28(YLR062C_d)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAJ1(YER048C)|FD-Score:-3.38|P-value:3.56E-4||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CBR1(YIL043C)|FD-Score:3.81|P-value:7.00E-5||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CCS1(YMR038C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CDC73(YLR418C)|FD-Score:-4.55|P-value:2.74E-6||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:COG7(YGL005C)|FD-Score:-3.73|P-value:9.39E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CWP2(YKL096W-A)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:ENV10(YLR065C)|FD-Score:-3.91|P-value:4.68E-5||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:FKS3(YMR306W)|FD-Score:6.32|P-value:1.27E-10||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN5(YGR252W)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GET2(YER083C)|FD-Score:-3.18|P-value:7.39E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GYP1(YOR070C)|FD-Score:-3.31|P-value:4.63E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HBN1(YCL026C-B_p)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HUG1(YML058W-A)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:ICP55(YER078C)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family Gene:IME4(YGL192W)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:IRC18(YJL037W)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:JJJ1(YNL227C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:LRS4(YDR439W)|FD-Score:-4.24|P-value:1.10E-5||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:LYS12(YIL094C)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Gene:MAC1(YMR021C)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MFM1(YPL060W)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MRPL8(YJL063C)|FD-Score:-4.21|P-value:1.25E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSY1(YPL097W)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:MTG1(YMR097C)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:MUD1(YBR119W)|FD-Score:3.85|P-value:5.93E-5||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:NKP2(YLR315W)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:OCA2(YNL056W)|FD-Score:-4.41|P-value:5.19E-6||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:OCA4(YCR095C_p)|FD-Score:-3.74|P-value:9.25E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OCH1(YGL038C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins Gene:PDR1(YGL013C)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PGD1(YGL025C)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PIN4(YBL051C)|FD-Score:3.81|P-value:6.89E-5||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PPH3(YDR075W)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Gene:PST2(YDR032C)|FD-Score:-3.15|P-value:8.05E-4||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:PTC1(YDL006W)|FD-Score:-3.29|P-value:5.10E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTR2(YKR093W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:PUS7(YOR243C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RAD26(YJR035W)|FD-Score:-3.27|P-value:5.44E-4||SGD DESC:Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein Gene:REI1(YBR267W)|FD-Score:7.28|P-value:1.63E-13||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RPL22A(YLR061W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPS25A(YGR027C)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RRP8(YDR083W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:RTT103(YDR289C)|FD-Score:-5.14|P-value:1.37E-7||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:RXT3(YDL076C)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SAS5(YOR213C)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SIP1(YDR422C)|FD-Score:-4.55|P-value:2.73E-6||SGD DESC:Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions Gene:SKY1(YMR216C)|FD-Score:-3.19|P-value:7.06E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SNQ2(YDR011W)|FD-Score:9.79|P-value:6.53E-23||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPL2(YHR136C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SPS19(YNL202W)|FD-Score:4.59|P-value:2.25E-6||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SQS1(YNL224C)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:SSN3(YPL042C)|FD-Score:-3.9|P-value:4.73E-5||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:TOM7(YNL070W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TOM70(YNL121C)|FD-Score:-3.87|P-value:5.48E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TRM2(YKR056W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:UBP1(YDL122W)|FD-Score:-3.68|P-value:1.19E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:UBP3(YER151C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:ULA1(YPL003W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:VAC14(YLR386W)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VMA1(YDL185W)|FD-Score:-3.2|P-value:6.83E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:-4.93|P-value:4.13E-7||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA16(YHR026W)|FD-Score:-3.35|P-value:4.03E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS61(YDR136C_d)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YDR034W-B(YDR034W-B_p)|FD-Score:4.48|P-value:3.66E-6||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR149C(YDR149C_d)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YGL214W(YGL214W_d)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Gene:YIL002W-A(YIL002W-A_p)|FD-Score:8.87|P-value:3.66E-19||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIL012W(YIL012W_d)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIR021W-A(YIR021W-A_p)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YKL162C-A(YKL162C-A_d)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:-5.7|P-value:5.90E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Putative protein of unknown function Gene:YMR247W-A(YMR247W-A_p)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Putative protein of unknown function Gene:YNL043C(YNL043C_d)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YNL226W(YNL226W_d)|FD-Score:4.62|P-value:1.96E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YNL228W(YNL228W_d)|FD-Score:4.8|P-value:8.12E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YOR200W(YOR200W_d)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YOR331C(YOR331C_d)|FD-Score:-3.79|P-value:7.55E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:ADE5,7(YGL234W)|FD-Score:3.75|P-value:8.75E-5||SGD DESC:Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities Gene:AEP1(YMR064W)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:ARO1(YDR127W)|FD-Score:-4.81|P-value:7.37E-7||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG16(YMR159C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BEM2(YER155C)|FD-Score:3.8|P-value:7.27E-5||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:BIO5(YNR056C)|FD-Score:3.1|P-value:9.55E-4||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BMH1(YER177W)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BRE5(YNR051C)|FD-Score:5.73|P-value:5.07E-9||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:BTS1(YPL069C)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD28(YLR062C_d)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAJ1(YER048C)|FD-Score:-3.38|P-value:3.56E-4||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CBR1(YIL043C)|FD-Score:3.81|P-value:7.00E-5||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CCS1(YMR038C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CDC73(YLR418C)|FD-Score:-4.55|P-value:2.74E-6||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:COG7(YGL005C)|FD-Score:-3.73|P-value:9.39E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CWP2(YKL096W-A)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:ENV10(YLR065C)|FD-Score:-3.91|P-value:4.68E-5||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:FKS3(YMR306W)|FD-Score:6.32|P-value:1.27E-10||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN5(YGR252W)|FD-Score:-3.12|P-value:9.10E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GET2(YER083C)|FD-Score:-3.18|P-value:7.39E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GYP1(YOR070C)|FD-Score:-3.31|P-value:4.63E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HBN1(YCL026C-B_p)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HUG1(YML058W-A)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:ICP55(YER078C)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family Gene:IME4(YGL192W)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:IRC18(YJL037W)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:JJJ1(YNL227C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis Gene:LRS4(YDR439W)|FD-Score:-4.24|P-value:1.10E-5||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:LYS12(YIL094C)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Gene:MAC1(YMR021C)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MFM1(YPL060W)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MRPL8(YJL063C)|FD-Score:-4.21|P-value:1.25E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSY1(YPL097W)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:MTG1(YMR097C)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:MUD1(YBR119W)|FD-Score:3.85|P-value:5.93E-5||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:NKP2(YLR315W)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:OCA2(YNL056W)|FD-Score:-4.41|P-value:5.19E-6||SGD DESC:Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene Gene:OCA4(YCR095C_p)|FD-Score:-3.74|P-value:9.25E-5||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OCH1(YGL038C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins Gene:PDR1(YGL013C)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PGD1(YGL025C)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PIN4(YBL051C)|FD-Score:3.81|P-value:6.89E-5||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PPH3(YDR075W)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Gene:PST2(YDR032C)|FD-Score:-3.15|P-value:8.05E-4||SGD DESC:Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication Gene:PTC1(YDL006W)|FD-Score:-3.29|P-value:5.10E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTR2(YKR093W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:PUS7(YOR243C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RAD26(YJR035W)|FD-Score:-3.27|P-value:5.44E-4||SGD DESC:Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein Gene:REI1(YBR267W)|FD-Score:7.28|P-value:1.63E-13||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RPL22A(YLR061W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:RPS25A(YGR027C)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RRP8(YDR083W)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1 Gene:RTT103(YDR289C)|FD-Score:-5.14|P-value:1.37E-7||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:RXT3(YDL076C)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SAS5(YOR213C)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SIP1(YDR422C)|FD-Score:-4.55|P-value:2.73E-6||SGD DESC:Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions Gene:SKY1(YMR216C)|FD-Score:-3.19|P-value:7.06E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SNQ2(YDR011W)|FD-Score:9.79|P-value:6.53E-23||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPL2(YHR136C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SPS19(YNL202W)|FD-Score:4.59|P-value:2.25E-6||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:SQS1(YNL224C)|FD-Score:3.82|P-value:6.76E-5||SGD DESC:Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:SSN3(YPL042C)|FD-Score:-3.9|P-value:4.73E-5||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:TOM7(YNL070W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TOM70(YNL121C)|FD-Score:-3.87|P-value:5.48E-5||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TRM2(YKR056W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:UBP1(YDL122W)|FD-Score:-3.68|P-value:1.19E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:UBP3(YER151C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:ULA1(YPL003W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:VAC14(YLR386W)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VMA1(YDL185W)|FD-Score:-3.2|P-value:6.83E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:-4.93|P-value:4.13E-7||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA16(YHR026W)|FD-Score:-3.35|P-value:4.03E-4||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS61(YDR136C_d)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YDR034W-B(YDR034W-B_p)|FD-Score:4.48|P-value:3.66E-6||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR149C(YDR149C_d)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YGL214W(YGL214W_d)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Gene:YIL002W-A(YIL002W-A_p)|FD-Score:8.87|P-value:3.66E-19||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIL012W(YIL012W_d)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIR021W-A(YIR021W-A_p)|FD-Score:3.11|P-value:9.23E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YKL162C-A(YKL162C-A_d)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:-5.7|P-value:5.90E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Putative protein of unknown function Gene:YMR247W-A(YMR247W-A_p)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Putative protein of unknown function Gene:YNL043C(YNL043C_d)|FD-Score:3.15|P-value:8.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YNL226W(YNL226W_d)|FD-Score:4.62|P-value:1.96E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YNL228W(YNL228W_d)|FD-Score:4.8|P-value:8.12E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YOR200W(YOR200W_d)|FD-Score:-3.09|P-value:9.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YOR331C(YOR331C_d)|FD-Score:-3.79|P-value:7.55E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W23.902.12E-12612.50LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YPL093W11.402.60E-301.00NOG1Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
YHR170W10.401.47E-252.31NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YER112W8.083.35E-162.00LSM4Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
YLR438C-A6.086.09E-100.62LSM3Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YHR107C5.462.45E-80.27CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YPL169C5.191.07E-71.27MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YHR007C3.924.41E-50.01ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YNL112W3.914.53E-50.02DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YCL052C3.894.91E-50.12PBN1Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X
YNL308C3.787.94E-50.13KRI1Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p
YDR240C3.641.34E-40.04SNU56Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
YKR081C3.601.57E-40.40RPF2Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles
YNL124W3.206.89E-40.04NAF1RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
YGR147C3.167.85E-40.03NAT2Protein with an apparent role in acetylation of N-terminal methionine residues

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR011W9.796.53E-23SNQ2Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species
YIL002W-A_p8.873.66E-19YIL002W-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YBR267W7.281.63E-13REI1Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
YMR306W6.321.27E-10FKS3Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YNR051C5.735.07E-9BRE5Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A
YNL228W_d4.808.12E-7YNL228W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1
YER177W4.621.94E-6BMH114-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
YNL226W_d4.621.96E-6YNL226W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C
YNL202W4.592.25E-6SPS19Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate
YDR034W-B_p4.483.66E-6YDR034W-B_pPredicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
YOL117W4.161.58E-5RRI2Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response
YPL060W4.141.75E-5MFM1Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p
YLR062C_d4.082.26E-5BUD28_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay
YBR119W3.855.93E-5MUD1U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing
YNL224C3.826.76E-5SQS1Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress

GO enrichment analysis for SGTC_97
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3882.34E-211SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.098901160S ribosome export
0.3475.23E-167SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.095890460S ribosome export
0.3244.10E-144SGTC_1829berberine 14.4 μMTimTec (Natural product derivative library)23530.160S ribosome export
0.3103.60E-132SGTC_620180-0423 3.2 μMChemDiv (Drug-like library)301020.10526360S ribosome export
0.3058.66E-128SGTC_21005377183 190.0 μMChembridge (Fragment library)28442020.11594260S ribosome export
0.3053.29E-127SGTC_15924',5'-dihydroxyflavone 8.8 μMTimTec (Pure natural product library)6886690.13043560S ribosome export
0.2922.61E-116SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.1560S ribosome export
0.2904.71E-115SGTC_1786berberine 21.6 μMMiscellaneous124560.160S ribosome export
0.2853.89E-111SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.11940360S ribosome export
0.2824.54E-108SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.14705960S ribosome export
0.2707.15E-99SGTC_1827berberine 7.2 μMTimTec (Natural product derivative library)6294130.160S ribosome export
0.2678.32E-97SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.160S ribosome export
0.2639.68E-94SGTC_8660495-0101 262.0 μMChemDiv (Drug-like library)6356940.10256460S ribosome export
0.2457.76E-81SGTC_30329089427 49.5 μMChembridge (Drug-like library)171737070.11111160S ribosome export
0.2404.51E-78SGTC_14043909-7820 48.3 μMChemDiv (Drug-like library)237413730.10389660S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_521r062-000513.8 μM0.5416672832033ChemDiv (Drug-like library)233.653723.1104
SGTC_11513909-831615.3 μM0.301587800431ChemDiv (Drug-like library)332.442064.50104
SGTC_3320913700535.14 μM0.2835824908713Chembridge (Drug-like library)335.4463.39704endomembrane recycling
SGTC_22587960471200 μM0.2786892974708Chembridge (Fragment library)298.834762.64715
SGTC_2822799471871.43 μM0.2535211018011Chembridge (Drug-like library)373.876463.58214
SGTC_1915st06710530.6 μM0.2469751TimTec (Natural product derivative library)209.243243.1602RNA pol III & RNase P/MRP
SGTC_21796298986200 μM0.237288738374Chembridge (Fragment library)218.235142.7312heme requiring
SGTC_1065prochlorperazine maleate2.48 μM0.2352946436057NIH Clinical Collection490.01482-1.33428
SGTC_411enrofloxacin100 μM0.2297371188Miscellaneous359.394683-0.71517DNA damage response
SGTC_14454239-015697.7 μM0.229734008346ChemDiv (Drug-like library)375.4123263.22816