1315-0384

4-bromo-2-ethoxy-6-[[3-(6-methyl-1,3-benzoxazol-2-yl)anilino]methylidene]cyclohexa-2,4-dien-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_971
Screen concentration 26.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 6769120
SMILES CCOC1=CC(=CC(=CNC2=CC=CC(=C2)C3=NC4=C(O3)C=C(C=C4)C)C1=O)Br
Standardized SMILES CCOc1cc(Br)cc(C=Nc2cccc(c2)c3oc4cc(C)ccc4n3)c1O
Molecular weight 451.3126
ALogP 4.53
H-bond donor count 1
H-bond acceptor count 5
Response signature cell wall signaling

Pool Growth Kinetics
% growth inhibition (Het. pool) 23.14
% growth inhibition (Hom. pool) 2.36


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6769120
Download HIP data (tab-delimited text)  (excel)
Gene:ATP16(YDL004W)|FD-Score:5.72|P-value:5.25E-9|Clearance:0.94||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:GLN4(YOR168W)|FD-Score:3.63|P-value:1.40E-4|Clearance:0.05||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:HEM2(YGL040C)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.11||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:MVD1(YNR043W)|FD-Score:3.86|P-value:5.75E-5|Clearance:0.17||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:PRP19(YLL036C)|FD-Score:4.78|P-value:8.70E-7|Clearance:0.18||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RRS1(YOR294W)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.3||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:TAF7(YMR227C)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.05||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:UTP6(YDR449C)|FD-Score:6.41|P-value:7.21E-11|Clearance:0.94||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YGR115C(YGR115C_d)|FD-Score:4.6|P-value:2.15E-6|Clearance:0.74||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YOR102W(YOR102W_d)|FD-Score:3.18|P-value:7.40E-4|Clearance:0.13||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex Gene:ATP16(YDL004W)|FD-Score:5.72|P-value:5.25E-9|Clearance:0.94||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:GLN4(YOR168W)|FD-Score:3.63|P-value:1.40E-4|Clearance:0.05||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:HEM2(YGL040C)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.11||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:MVD1(YNR043W)|FD-Score:3.86|P-value:5.75E-5|Clearance:0.17||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:PRP19(YLL036C)|FD-Score:4.78|P-value:8.70E-7|Clearance:0.18||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RRS1(YOR294W)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.3||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:TAF7(YMR227C)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.05||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:UTP6(YDR449C)|FD-Score:6.41|P-value:7.21E-11|Clearance:0.94||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YGR115C(YGR115C_d)|FD-Score:4.6|P-value:2.15E-6|Clearance:0.74||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YOR102W(YOR102W_d)|FD-Score:3.18|P-value:7.40E-4|Clearance:0.13||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6769120
Download HOP data (tab-delimited text)  (excel)
Gene:AGC1(YPR021C)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:AHC1(YOR023C)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:AIM18(YHR198C)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:AIM45(YPR004C)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:AIM9(YER080W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:API2(YDR525W_d)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:ARO3(YDR035W)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG7(YHR171W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:BCK1(YJL095W)|FD-Score:8.66|P-value:2.28E-18||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BDS1(YOL164W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BRE4(YDL231C)|FD-Score:5.1|P-value:1.66E-7||SGD DESC:Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport Gene:BRE5(YNR051C)|FD-Score:5.4|P-value:3.28E-8||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:BTN2(YGR142W)|FD-Score:4.9|P-value:4.74E-7||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:CCM1(YGR150C)|FD-Score:-4.06|P-value:2.44E-5||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CKA1(YIL035C)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CPD1(YGR247W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress Gene:DUG3(YNL191W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ERF2(YLR246W)|FD-Score:-3.86|P-value:5.78E-5||SGD DESC:Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole Gene:FAR7(YFR008W)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FMP37(YGL080W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia Gene:GCN2(YDR283C)|FD-Score:4.97|P-value:3.40E-7||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN5(YGR252W)|FD-Score:4.64|P-value:1.78E-6||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:HAL9(YOL089C)|FD-Score:3.95|P-value:3.83E-5||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:IFM1(YOL023W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IRS4(YKR019C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JJJ3(YJR097W)|FD-Score:5.1|P-value:1.66E-7||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KAR9(YPL269W)|FD-Score:3.89|P-value:5.05E-5||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:LDB16(YCL005W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LIP2(YLR239C)|FD-Score:4.5|P-value:3.34E-6||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:LST7(YGR057C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MFA1(YDR461W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 Gene:MPC54(YOR177C)|FD-Score:5.23|P-value:8.40E-8||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MSH3(YCR092C)|FD-Score:4.44|P-value:4.46E-6||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:NHP10(YDL002C)|FD-Score:3.76|P-value:8.62E-5||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NMA2(YGR010W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in de novo and salvage synthesis of NAD(+); NMA2 has a paralog, NMA1, that arose from the whole genome duplication Gene:PHO4(YFR034C)|FD-Score:3.99|P-value:3.24E-5||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PHO86(YJL117W)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PTC1(YDL006W)|FD-Score:3.85|P-value:5.79E-5||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTM1(YKL039W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication Gene:ROT2(YBR229C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RSB1(YOR049C)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane Gene:SCS22(YBL091C-A)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; similar to D. melanogaster inturned protein; SCS22 has a paralog, SCS2, that arose from the whole genome duplication Gene:SLM6(YBR266C_d)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SLT2(YHR030C)|FD-Score:5.07|P-value:2.02E-7||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF2(YOR290C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPO21(YOL091W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SPT3(YDR392W)|FD-Score:-3.19|P-value:7.02E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRB2(YHR041C)|FD-Score:-4.06|P-value:2.46E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:TAT1(YBR069C)|FD-Score:4.02|P-value:2.87E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TAT2(YOL020W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TRP1(YDR007W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.28|P-value:1.72E-10||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBP1(YDL122W)|FD-Score:4.95|P-value:3.75E-7||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:UBP12(YJL197W)|FD-Score:3.82|P-value:6.77E-5||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VPS1(YKR001C)|FD-Score:-4.53|P-value:2.97E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS25(YJR102C)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:YAK1(YJL141C)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose Gene:YBL053W(YBL053W_d)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR043C(YCR043C_p)|FD-Score:3.74|P-value:9.21E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:4.68|P-value:1.40E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YEF1(YEL041W)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YER097W(YER097W_d)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER156C(YER156C_p)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YGL117W(YGL117W_p)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Putative protein of unknown function Gene:YGP1(YNL160W)|FD-Score:-3.17|P-value:7.70E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YHR095W(YHR095W_d)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL218W(YJL218W_p)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene Gene:YKL136W(YKL136W_d)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YML089C(YML089C_d)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Gene:YMR173W-A(YMR173W-A_d)|FD-Score:5.13|P-value:1.47E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YNL170W(YNL170W_d)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR063W(YNR063W_p)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Putative zinc-cluster protein of unknown function Gene:YOR121C(YOR121C_d)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YPL107W(YPL107W_p)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene Gene:YPR123C(YPR123C_d)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR127W(YPR127W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:AGC1(YPR021C)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:AHC1(YOR023C)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:AIM18(YHR198C)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:AIM45(YPR004C)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:AIM9(YER080W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:API2(YDR525W_d)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:ARO3(YDR035W)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG7(YHR171W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:BCK1(YJL095W)|FD-Score:8.66|P-value:2.28E-18||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BDS1(YOL164W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BRE4(YDL231C)|FD-Score:5.1|P-value:1.66E-7||SGD DESC:Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport Gene:BRE5(YNR051C)|FD-Score:5.4|P-value:3.28E-8||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:BTN2(YGR142W)|FD-Score:4.9|P-value:4.74E-7||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:CCM1(YGR150C)|FD-Score:-4.06|P-value:2.44E-5||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CKA1(YIL035C)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CPD1(YGR247W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress Gene:DUG3(YNL191W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ERF2(YLR246W)|FD-Score:-3.86|P-value:5.78E-5||SGD DESC:Subunit of a palmitoyltransferase, composed of Erf2p and Shr5p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole Gene:FAR7(YFR008W)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:FMP37(YGL080W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia Gene:GCN2(YDR283C)|FD-Score:4.97|P-value:3.40E-7||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN5(YGR252W)|FD-Score:4.64|P-value:1.78E-6||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:HAL9(YOL089C)|FD-Score:3.95|P-value:3.83E-5||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:IFM1(YOL023W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IRS4(YKR019C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:JJJ3(YJR097W)|FD-Score:5.1|P-value:1.66E-7||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KAR9(YPL269W)|FD-Score:3.89|P-value:5.05E-5||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:LDB16(YCL005W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:LIP2(YLR239C)|FD-Score:4.5|P-value:3.34E-6||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:LST7(YGR057C)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MFA1(YDR461W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 Gene:MPC54(YOR177C)|FD-Score:5.23|P-value:8.40E-8||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MSH3(YCR092C)|FD-Score:4.44|P-value:4.46E-6||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:NHP10(YDL002C)|FD-Score:3.76|P-value:8.62E-5||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NMA2(YGR010W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in de novo and salvage synthesis of NAD(+); NMA2 has a paralog, NMA1, that arose from the whole genome duplication Gene:PHO4(YFR034C)|FD-Score:3.99|P-value:3.24E-5||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PHO86(YJL117W)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PTC1(YDL006W)|FD-Score:3.85|P-value:5.79E-5||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTM1(YKL039W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication Gene:ROT2(YBR229C)|FD-Score:3.21|P-value:6.66E-4||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RSB1(YOR049C)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane Gene:SCS22(YBL091C-A)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; similar to D. melanogaster inturned protein; SCS22 has a paralog, SCS2, that arose from the whole genome duplication Gene:SLM6(YBR266C_d)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SLT2(YHR030C)|FD-Score:5.07|P-value:2.02E-7||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF2(YOR290C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPO21(YOL091W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SPT3(YDR392W)|FD-Score:-3.19|P-value:7.02E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRB2(YHR041C)|FD-Score:-4.06|P-value:2.46E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:TAT1(YBR069C)|FD-Score:4.02|P-value:2.87E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TAT2(YOL020W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TRP1(YDR007W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.28|P-value:1.72E-10||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBP1(YDL122W)|FD-Score:4.95|P-value:3.75E-7||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:UBP12(YJL197W)|FD-Score:3.82|P-value:6.77E-5||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VPS1(YKR001C)|FD-Score:-4.53|P-value:2.97E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS25(YJR102C)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:YAK1(YJL141C)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose Gene:YBL053W(YBL053W_d)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCR043C(YCR043C_p)|FD-Score:3.74|P-value:9.21E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:4.68|P-value:1.40E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YEF1(YEL041W)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YER097W(YER097W_d)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER156C(YER156C_p)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YGL117W(YGL117W_p)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Putative protein of unknown function Gene:YGP1(YNL160W)|FD-Score:-3.17|P-value:7.70E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YHR095W(YHR095W_d)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL218W(YJL218W_p)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene Gene:YKL136W(YKL136W_d)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C Gene:YML089C(YML089C_d)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Gene:YMR173W-A(YMR173W-A_d)|FD-Score:5.13|P-value:1.47E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YNL170W(YNL170W_d)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNR063W(YNR063W_p)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Putative zinc-cluster protein of unknown function Gene:YOR121C(YOR121C_d)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YPL107W(YPL107W_p)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene Gene:YPR123C(YPR123C_d)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR127W(YPR127W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR449C6.417.21E-110.94UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YDL004W5.725.25E-90.94ATP16Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
YLL036C4.788.70E-70.18PRP19Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain
YGR115C_d4.602.15E-60.74YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YNR043W3.865.75E-50.17MVD1Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer
YMR227C3.681.16E-40.05TAF7TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation
YOR168W3.631.40E-40.05GLN4Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
YGL040C3.591.68E-40.11HEM2Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
YOR294W3.482.54E-40.30RRS1Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
YOR102W_d3.187.40E-40.13YOR102W_dDubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex
YNL026W3.050.001130.15SAM50Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85
YKL193C2.900.001860.12SDS22Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function
YJR112W2.790.002670.03NNF1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
YKL196C2.750.002970.04YKT6Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus
YGR094W2.710.003380.05VAS1Mitochondrial and cytoplasmic valyl-tRNA synthetase

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL095W8.662.28E-18BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YKL211C6.281.72E-10TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YNR051C5.403.28E-8BRE5Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A
YOR177C5.238.40E-8MPC54Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate
YMR173W-A_d5.131.47E-7YMR173W-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W
YDL231C5.101.66E-7BRE4Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport
YJR097W5.101.66E-7JJJ3Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4
YHR030C5.072.02E-7SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YDR283C4.973.40E-7GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YDL122W4.953.75E-7UBP1Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains
YGR142W4.904.74E-7BTN2v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication
YDR008C_d4.681.40E-6YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YGR252W4.641.78E-6GCN5Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation
YLR239C4.503.34E-6LIP2Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
YCR092C4.444.46E-6MSH3Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability

GO enrichment analysis for SGTC_971
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2651.06E-95SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.10101
0.2111.57E-60SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.046729
0.2016.63E-55SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0930233
0.2001.94E-54SGTC_1671st016596 74.5 μMTimTec (Natural product derivative library)28291670.0947368
0.1964.07E-52SGTC_14274048-4900 550.0 nMChemDiv (Drug-like library)241325860.0595238
0.1951.13E-51SGTC_2776terbinafine 19.9 μMNIH Clinical Collection52824810.0888889cell wall signaling
0.1924.30E-50SGTC_9052-aminofluorene 215.0 μMChemDiv (Drug-like library)15390.0649351cell wall signaling
0.1904.50E-49SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.0860215
0.1907.03E-49SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0519481
0.1891.63E-48SGTC_2524avocatin b 10.6 μMMicrosource (Natural product library)67107480.037037
0.1885.75E-48SGTC_465cgp-37157 41.0 μMICCB bioactive library26880.0543478
0.1865.02E-47SGTC_22727805186 180.0 μMChembridge (Fragment library)29560620.0555556cell wall signaling
0.1852.17E-46SGTC_2409alverine citrate 64.0 μMMiscellaneous217180.0933333
0.1843.31E-46SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.103093
0.1839.95E-46SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0430108

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_5741315-039740 μM0.7164186794277ChemDiv (Drug-like library)437.285984.17615
SGTC_170960-027649.81 μM0.6164386821114ChemDiv (Drug-like library)471.731044.70315
SGTC_560957-036583.25 μM0.5135146752360ChemDiv (Drug-like library)453.285383.67416Golgi
SGTC_3111315-013658 μM0.4857146759207ChemDiv (Drug-like library)372.6166833.45814
SGTC_6841140-0066328 μM0.4756813534ChemDiv (Drug-like library)406.861564.73715Golgi
SGTC_1100850-0284111.64 μM0.4520553906265ChemDiv (Drug-like library)391.26066.11103
SGTC_7771315-0167517 μM0.4266674221767ChemDiv (Drug-like library)381.254666.69203Golgi
SGTC_5611550-033072.9 μM0.4146346812934ChemDiv (Drug-like library)471.731044.38915
SGTC_1380850-025940.92 μM0.4102566813004ChemDiv (Drug-like library)407.265.04614cell wall
SGTC_5860957-02658.15 μM0.397596752309ChemDiv (Drug-like library)388.41592.83726cell wall