1416-0512

N'-[(3-methoxy-6-oxocyclohexa-2,4-dien-1-ylidene)methyl]benzohydrazide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_978
Screen concentration 87.4 μM
Source ChemDiv (Drug-like library)
PubChem CID 6814003
SMILES COC1=CC(=CNNC(=O)C2=CC=CC=C2)C(=O)C=C1
Standardized SMILES COc1ccc(O)c(C=NNC(=O)c2ccccc2)c1
Molecular weight 270.2833
ALogP 0.69
H-bond donor count 2
H-bond acceptor count 4
Response signature redox potentiating

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.48
% growth inhibition (Hom. pool) 8.72


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6814003
Download HIP data (tab-delimited text)  (excel)
Gene:ATM1(YMR301C)|FD-Score:-4|P-value:3.17E-5|Clearance:0||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:CDC37(YDR168W)|FD-Score:3.84|P-value:6.06E-5|Clearance:0.02||SGD DESC:Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding Gene:DBP6(YNR038W)|FD-Score:3.38|P-value:3.69E-4|Clearance:0.13||SGD DESC:Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family Gene:GLC7(YER133W)|FD-Score:3.48|P-value:2.47E-4|Clearance:0.11||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:NOC3(YLR002C)|FD-Score:3.25|P-value:5.85E-4|Clearance:0.15||SGD DESC:Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing Gene:RPA43(YOR340C)|FD-Score:4.16|P-value:1.61E-5|Clearance:0.04||SGD DESC:RNA polymerase I subunit A43 Gene:RPP1(YHR062C)|FD-Score:-3.43|P-value:2.99E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SCL1(YGL011C)|FD-Score:-3.4|P-value:3.36E-4|Clearance:0||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SEC27(YGL137W)|FD-Score:3.1|P-value:9.71E-4|Clearance:0.11||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SEC61(YLR378C)|FD-Score:5.24|P-value:8.09E-8|Clearance:0.69||SGD DESC:Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER Gene:SRB6(YBR253W)|FD-Score:4.89|P-value:5.00E-7|Clearance:0.69||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:SWD2(YKL018W)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.08||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:TOM40(YMR203W)|FD-Score:4.87|P-value:5.49E-7|Clearance:0.69||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TOR2(YKL203C)|FD-Score:3.83|P-value:6.51E-5|Clearance:0.27||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication Gene:TRE2(YOR256C)|FD-Score:4.96|P-value:3.53E-7|Clearance:0.69||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:YDL163W(YDL163W_d)|FD-Score:5.71|P-value:5.74E-9|Clearance:0.69||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YDL196W(YDL196W_d)|FD-Score:4.11|P-value:1.94E-5|Clearance:0.27||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YPL142C(YPL142C_d)|FD-Score:4.18|P-value:1.44E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:ATM1(YMR301C)|FD-Score:-4|P-value:3.17E-5|Clearance:0||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:CDC37(YDR168W)|FD-Score:3.84|P-value:6.06E-5|Clearance:0.02||SGD DESC:Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding Gene:DBP6(YNR038W)|FD-Score:3.38|P-value:3.69E-4|Clearance:0.13||SGD DESC:Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family Gene:GLC7(YER133W)|FD-Score:3.48|P-value:2.47E-4|Clearance:0.11||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:NOC3(YLR002C)|FD-Score:3.25|P-value:5.85E-4|Clearance:0.15||SGD DESC:Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing Gene:RPA43(YOR340C)|FD-Score:4.16|P-value:1.61E-5|Clearance:0.04||SGD DESC:RNA polymerase I subunit A43 Gene:RPP1(YHR062C)|FD-Score:-3.43|P-value:2.99E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SCL1(YGL011C)|FD-Score:-3.4|P-value:3.36E-4|Clearance:0||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SEC27(YGL137W)|FD-Score:3.1|P-value:9.71E-4|Clearance:0.11||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SEC61(YLR378C)|FD-Score:5.24|P-value:8.09E-8|Clearance:0.69||SGD DESC:Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER Gene:SRB6(YBR253W)|FD-Score:4.89|P-value:5.00E-7|Clearance:0.69||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:SWD2(YKL018W)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.08||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:TOM40(YMR203W)|FD-Score:4.87|P-value:5.49E-7|Clearance:0.69||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TOR2(YKL203C)|FD-Score:3.83|P-value:6.51E-5|Clearance:0.27||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication Gene:TRE2(YOR256C)|FD-Score:4.96|P-value:3.53E-7|Clearance:0.69||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:YDL163W(YDL163W_d)|FD-Score:5.71|P-value:5.74E-9|Clearance:0.69||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YDL196W(YDL196W_d)|FD-Score:4.11|P-value:1.94E-5|Clearance:0.27||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YPL142C(YPL142C_d)|FD-Score:4.18|P-value:1.44E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6814003
Download HOP data (tab-delimited text)  (excel)
Gene:ALG8(YOR067C)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:ATG13(YPR185W)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling Gene:ATP14(YLR295C)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:BCH1(YMR237W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BDS1(YOL164W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BEM4(YPL161C)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BTN2(YGR142W)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CCC2(YDR270W)|FD-Score:4.46|P-value:4.11E-6||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CCR4(YAL021C)|FD-Score:5.25|P-value:7.56E-8||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CEX1(YOR112W)|FD-Score:4.28|P-value:9.32E-6||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:CKA1(YIL035C)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:COA3(YJL062W-A)|FD-Score:-4.74|P-value:1.07E-6||SGD DESC:Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly Gene:DAL81(YIR023W)|FD-Score:-4.12|P-value:1.89E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBF2(YGR092W)|FD-Score:4.64|P-value:1.78E-6||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DBR1(YKL149C)|FD-Score:4.37|P-value:6.15E-6||SGD DESC:RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition Gene:FET4(YMR319C)|FD-Score:9.69|P-value:1.68E-22||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GCS1(YDL226C)|FD-Score:5.03|P-value:2.50E-7||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDE1(YPL110C)|FD-Score:4.72|P-value:1.15E-6||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:GIP3(YPL137C)|FD-Score:5.04|P-value:2.38E-7||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:GPR1(YDL035C)|FD-Score:4.51|P-value:3.30E-6||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HAP2(YGL237C)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding Gene:HXT5(YHR096C)|FD-Score:-4.4|P-value:5.35E-6||SGD DESC:Hexose transporter with moderate affinity for glucose; induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs; HXT5 has a paralog, HXT3, that arose from the whole genome duplication Gene:IRC3(YDR332W)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:IRS4(YKR019C)|FD-Score:-5.54|P-value:1.51E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISM1(YPL040C)|FD-Score:-4.72|P-value:1.19E-6||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:KAP114(YGL241W)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress Gene:LEU9(YOR108W)|FD-Score:4.43|P-value:4.78E-6||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:MDG1(YNL173C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MLH2(YLR035C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p Gene:MVB12(YGR206W)|FD-Score:5.13|P-value:1.46E-7||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:NGG1(YDR176W)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:PAU23(YLR037C)|FD-Score:5.65|P-value:8.23E-9||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PEX10(YDR265W)|FD-Score:4.27|P-value:9.68E-6||SGD DESC:Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders Gene:PEX22(YAL055W)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:PEX27(YOR193W)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PRO2(YOR323C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRS3(YHL011C)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PRS4(YBL068W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication Gene:PTM1(YKL039W)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication Gene:RCK1(YGL158W)|FD-Score:4.37|P-value:6.08E-6||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:REC107(YJR021C)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation Gene:RIM101(YHL027W)|FD-Score:7.96|P-value:8.93E-16||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM13(YMR154C)|FD-Score:8.7|P-value:1.71E-18||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM21(YNL294C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RIM8(YGL045W)|FD-Score:10.1|P-value:4.23E-24||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RIM9(YMR063W)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RKM1(YPL208W)|FD-Score:4.83|P-value:6.70E-7||SGD DESC:SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp) Gene:ROT2(YBR229C)|FD-Score:4.99|P-value:3.05E-7||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:SNF7(YLR025W)|FD-Score:3.97|P-value:3.52E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNX3(YOR357C)|FD-Score:4.84|P-value:6.43E-7||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SOD2(YHR008C)|FD-Score:3.86|P-value:5.76E-5||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SPT3(YDR392W)|FD-Score:-3.16|P-value:7.81E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRO77(YBL106C)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:STB2(YMR053C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:TAT1(YBR069C)|FD-Score:-4.49|P-value:3.59E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA8(YAL064C-A_p)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TRF5(YNL299W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:TRP3(YKL211C)|FD-Score:-3.88|P-value:5.31E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VPS1(YKR001C)|FD-Score:-3.77|P-value:8.11E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS20(YMR077C)|FD-Score:6.41|P-value:7.14E-11||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS25(YJR102C)|FD-Score:5.3|P-value:5.64E-8||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS36(YLR417W)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:WSC2(YNL283C)|FD-Score:4.93|P-value:4.01E-7||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YDL062W(YDL062W_d)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential Gene:YDL119C(YDL119C_p)|FD-Score:3.92|P-value:4.41E-5||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YGR051C(YGR051C_d)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene Gene:YGR122W(YGR122W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YHP1(YDR451C)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YIL054W(YIL054W_p)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YLR416C(YLR416C_d)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR013W-A(YMR013W-A_d)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 Gene:YNL276C(YNL276C_d)|FD-Score:-4.51|P-value:3.30E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YOR331C(YOR331C_d)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:YPL185W(YPL185W_d)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:YPR039W(YPR039W_d)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:ALG8(YOR067C)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p Gene:ATG13(YPR185W)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling Gene:ATP14(YLR295C)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:BCH1(YMR237W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BDS1(YOL164W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BEM4(YPL161C)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BTN2(YGR142W)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; BTN2 has a paralog, CUR1, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CCC2(YDR270W)|FD-Score:4.46|P-value:4.11E-6||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CCR4(YAL021C)|FD-Score:5.25|P-value:7.56E-8||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CEX1(YOR112W)|FD-Score:4.28|P-value:9.32E-6||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:CKA1(YIL035C)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:COA3(YJL062W-A)|FD-Score:-4.74|P-value:1.07E-6||SGD DESC:Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly Gene:DAL81(YIR023W)|FD-Score:-4.12|P-value:1.89E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBF2(YGR092W)|FD-Score:4.64|P-value:1.78E-6||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DBR1(YKL149C)|FD-Score:4.37|P-value:6.15E-6||SGD DESC:RNA lariat debranching enzyme, involved in intron turnover; required for efficient Ty1 transposition Gene:FET4(YMR319C)|FD-Score:9.69|P-value:1.68E-22||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GCS1(YDL226C)|FD-Score:5.03|P-value:2.50E-7||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDE1(YPL110C)|FD-Score:4.72|P-value:1.15E-6||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:GIP3(YPL137C)|FD-Score:5.04|P-value:2.38E-7||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:GPR1(YDL035C)|FD-Score:4.51|P-value:3.30E-6||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HAP2(YGL237C)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding Gene:HXT5(YHR096C)|FD-Score:-4.4|P-value:5.35E-6||SGD DESC:Hexose transporter with moderate affinity for glucose; induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs; HXT5 has a paralog, HXT3, that arose from the whole genome duplication Gene:IRC3(YDR332W)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:IRS4(YKR019C)|FD-Score:-5.54|P-value:1.51E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISM1(YPL040C)|FD-Score:-4.72|P-value:1.19E-6||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:KAP114(YGL241W)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress Gene:LEU9(YOR108W)|FD-Score:4.43|P-value:4.78E-6||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:MDG1(YNL173C)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Plasma membrane protein involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations Gene:MLH2(YLR035C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p Gene:MVB12(YGR206W)|FD-Score:5.13|P-value:1.46E-7||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:NGG1(YDR176W)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:PAU23(YLR037C)|FD-Score:5.65|P-value:8.23E-9||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PEX10(YDR265W)|FD-Score:4.27|P-value:9.68E-6||SGD DESC:Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders Gene:PEX22(YAL055W)|FD-Score:-3.15|P-value:8.30E-4||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:PEX27(YOR193W)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Peripheral peroxisomal membrane protein involved in controlling peroxisome size and number, interacts with homologous protein Pex25p Gene:PRO2(YOR323C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRS3(YHL011C)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PRS4(YBL068W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication Gene:PTM1(YKL039W)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication Gene:RCK1(YGL158W)|FD-Score:4.37|P-value:6.08E-6||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:REC107(YJR021C)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation Gene:RIM101(YHL027W)|FD-Score:7.96|P-value:8.93E-16||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM13(YMR154C)|FD-Score:8.7|P-value:1.71E-18||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM21(YNL294C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RIM8(YGL045W)|FD-Score:10.1|P-value:4.23E-24||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RIM9(YMR063W)|FD-Score:3.92|P-value:4.39E-5||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RKM1(YPL208W)|FD-Score:4.83|P-value:6.70E-7||SGD DESC:SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp) Gene:ROT2(YBR229C)|FD-Score:4.99|P-value:3.05E-7||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:SNF7(YLR025W)|FD-Score:3.97|P-value:3.52E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNX3(YOR357C)|FD-Score:4.84|P-value:6.43E-7||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SOD2(YHR008C)|FD-Score:3.86|P-value:5.76E-5||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SPT3(YDR392W)|FD-Score:-3.16|P-value:7.81E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRO77(YBL106C)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:STB2(YMR053C)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:TAT1(YBR069C)|FD-Score:-4.49|P-value:3.59E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA8(YAL064C-A_p)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TRF5(YNL299W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:TRP3(YKL211C)|FD-Score:-3.88|P-value:5.31E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:VPS1(YKR001C)|FD-Score:-3.77|P-value:8.11E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS20(YMR077C)|FD-Score:6.41|P-value:7.14E-11||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS25(YJR102C)|FD-Score:5.3|P-value:5.64E-8||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS28(YPL065W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS36(YLR417W)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:WSC2(YNL283C)|FD-Score:4.93|P-value:4.01E-7||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YDL062W(YDL062W_d)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential Gene:YDL119C(YDL119C_p)|FD-Score:3.92|P-value:4.41E-5||SGD DESC:Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria Gene:YGR051C(YGR051C_d)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene Gene:YGR122W(YGR122W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YHP1(YDR451C)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YIL054W(YIL054W_p)|FD-Score:-3.41|P-value:3.21E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YLR416C(YLR416C_d)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR013W-A(YMR013W-A_d)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 Gene:YNL276C(YNL276C_d)|FD-Score:-4.51|P-value:3.30E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YOR331C(YOR331C_d)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:YPL185W(YPL185W_d)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:YPR039W(YPR039W_d)|FD-Score:3.17|P-value:7.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL163W_d5.715.74E-90.69YDL163W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase
YLR378C5.248.09E-80.69SEC61Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER
YOR256C4.963.53E-70.69TRE2Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
YBR253W4.895.00E-70.69SRB6Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YMR203W4.875.49E-70.69TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YPL142C_d4.181.44E-50.03YPL142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
YOR340C4.161.61E-50.04RPA43RNA polymerase I subunit A43
YDL196W_d4.111.94E-50.27YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YDR168W3.846.06E-50.02CDC37Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding
YKL203C3.836.51E-50.27TOR2PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
YKL018W3.561.85E-40.08SWD2Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination
YER133W3.482.47E-40.11GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YNR038W3.383.69E-40.13DBP6Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family
YLR002C3.255.85E-40.15NOC3Component of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing
YGL137W3.109.71E-40.11SEC27Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL045W10.104.23E-24RIM8Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
YMR319C9.691.68E-22FET4Low-affinity Fe(II) transporter of the plasma membrane
YMR154C8.701.71E-18RIM13Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
YHL027W7.968.93E-16RIM101Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC
YMR077C6.417.14E-11VPS20Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes
YLR037C5.658.23E-9PAU23Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth
YJR102C5.305.64E-8VPS25Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
YAL021C5.257.56E-8CCR4Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening
YOR323C5.191.03E-7PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YJR021C5.191.06E-7REC107Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation
YGR206W5.131.46E-7MVB12ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
YPL137C5.042.38E-7GIP3Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication
YDL226C5.032.50E-7GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YBR229C4.993.05E-7ROT2Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
YNL283C4.934.01E-7WSC2Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response

GO enrichment analysis for SGTC_978
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3327.53E-152SGTC_18845633444 25.0 μMMiscellaneous57242390.176471redox potentiating
0.2481.29E-83SGTC_525k781-2675 26.7 μMChemDiv (Drug-like library)36511180.213115redox potentiating
0.2421.44E-79SGTC_13171273-0028 910.0 nMChemDiv (Drug-like library)59178030.129032azole & statin
0.2232.96E-67SGTC_5761073-0113 22.7 μMChemDiv (Drug-like library)54395630.256757redox potentiating
0.2001.89E-54SGTC_11911487-1166 10.6 μMChemDiv (Drug-like library)39557710.153846azole & statin
0.1963.58E-52SGTC_8620452-0056 10.2 μMChemDiv (Drug-like library)15753960.056338azole & statin
0.1952.06E-51SGTC_1788fluconazole 20.0 μMMiscellaneous33650.0714286azole & statin
0.1942.28E-51SGTC_10441222-0021 1.8 μMChemDiv (Drug-like library)43067030.112676heme biosynthesis & mitochondrial translocase
0.1882.79E-48SGTC_24855633444 17.8 μMMiscellaneous57242390.176471redox potentiating
0.1837.22E-46SGTC_6604031-1742 102.0 μMChemDiv (Drug-like library)67387060.375redox potentiating
0.1761.72E-42SGTC_31109122132 49.5 μMChembridge (Drug-like library)9183170.119403redox potentiating
0.1742.68E-41SGTC_227fluconazole 33.5 μMMiscellaneous33650.0714286azole & statin
0.1719.26E-40SGTC_1962st076595 64.5 μMTimTec (Natural product derivative library)2796190.119403redox potentiating
0.1673.25E-38SGTC_1593justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 54.9 μMTimTec (Pure natural product library)4428820.12redox potentiating
0.1631.88E-36SGTC_29289011484 9.8 μMChembridge (Drug-like library)29947510.0657895heme biosynthesis & mitochondrial translocase

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_30833-058795.58 μM0.6511635382644ChemDiv (Drug-like library)240.257281.00623redox potentiating
SGTC_11413253-038952.6 μM0.4821435402031ChemDiv (Drug-like library)290.337562.60315
SGTC_1731st03596463 μM0.4727276739575TimTec (Natural product derivative library)317.253640.26547
SGTC_2477596768625.23 μM0.470588776118Miscellaneous270.279962.89614
SGTC_11780988-0033191 μM0.4642865392207ChemDiv (Drug-like library)349.179323.17324
SGTC_11841269-1718139 μM0.4181825403821ChemDiv (Drug-like library)260.311582.6214
SGTC_563tetrahydrosecaminediol614 μM0.4334274ChemDiv (Drug-like library)624.898248.24734amide catabolism
SGTC_12201838-0075193 μM0.45529645ChemDiv (Drug-like library)379.20533.15625
SGTC_13301469-005461.8 μM0.3970597102664ChemDiv (Drug-like library)382.80372.93536iron homeostasis
SGTC_12191469-021133.1 μM0.3870976822282ChemDiv (Drug-like library)341.361162.14925