1426-2828

N-(2,4-dichlorophenyl)-2-methylpyrazole-3-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_979
Screen concentration 121.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 747872
SMILES CN1C(=CC=N1)C(=O)NC2=C(C=C(C=C2)Cl)Cl
Standardized SMILES Cn1nccc1C(=O)Nc2ccc(Cl)cc2Cl
Molecular weight 270.1147
ALogP 2.88
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 15.14
% growth inhibition (Hom. pool) 7.81


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 747872
Download HIP data (tab-delimited text)  (excel)
Gene:CDC1(YDR182W)|FD-Score:3.34|P-value:4.13E-4|Clearance:0.04||SGD DESC:Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution Gene:CFD1(YIL003W)|FD-Score:3.19|P-value:7.10E-4|Clearance:0.13||SGD DESC:Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol Gene:HEM1(YDR232W)|FD-Score:3.37|P-value:3.72E-4|Clearance:0.03||SGD DESC:5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p Gene:NOC4(YPR144C)|FD-Score:3.25|P-value:5.74E-4|Clearance:0.06||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:NOP1(YDL014W)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.03||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:RDS3(YPR094W)|FD-Score:3.3|P-value:4.84E-4|Clearance:0.05||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:TOM40(YMR203W)|FD-Score:-3.99|P-value:3.32E-5|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UTP6(YDR449C)|FD-Score:3.57|P-value:1.80E-4|Clearance:0.17||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:CDC1(YDR182W)|FD-Score:3.34|P-value:4.13E-4|Clearance:0.04||SGD DESC:Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution Gene:CFD1(YIL003W)|FD-Score:3.19|P-value:7.10E-4|Clearance:0.13||SGD DESC:Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol Gene:HEM1(YDR232W)|FD-Score:3.37|P-value:3.72E-4|Clearance:0.03||SGD DESC:5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p Gene:NOC4(YPR144C)|FD-Score:3.25|P-value:5.74E-4|Clearance:0.06||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:NOP1(YDL014W)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.03||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:RDS3(YPR094W)|FD-Score:3.3|P-value:4.84E-4|Clearance:0.05||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance Gene:TOM40(YMR203W)|FD-Score:-3.99|P-value:3.32E-5|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UTP6(YDR449C)|FD-Score:3.57|P-value:1.80E-4|Clearance:0.17||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 747872
Download HOP data (tab-delimited text)  (excel)
Gene:AAP1(YHR047C)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:AEP1(YMR064W)|FD-Score:5.53|P-value:1.59E-8||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AFI1(YOR129C)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Arf3p polarization-specific docking factor, required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p Gene:APL1(YJR005W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:ATP5(YDR298C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:AYR1(YIL124W)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BEM2(YER155C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:CCM1(YGR150C)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CDA2(YLR308W)|FD-Score:4.75|P-value:1.04E-6||SGD DESC:Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:CDH1(YGL003C)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:CEM1(YER061C)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:COG6(YNL041C)|FD-Score:6.19|P-value:3.04E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUP2(YGL166W)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:DCS1(YLR270W)|FD-Score:6.18|P-value:3.14E-10||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:FRE6(YLL051C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels Gene:GCN2(YDR283C)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GIS3(YLR094C)|FD-Score:3.91|P-value:4.57E-5||SGD DESC:Protein of unknown function Gene:HBS1(YKR084C)|FD-Score:4.28|P-value:9.23E-6||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HNT3(YOR258W)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HTL1(YCR020W-B)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:ISM1(YPL040C)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:JJJ3(YJR097W)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KAP122(YGL016W)|FD-Score:-3.79|P-value:7.49E-5||SGD DESC:Karyopherin beta, responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance Gene:LCB5(YLR260W)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LEU9(YOR108W)|FD-Score:4.95|P-value:3.76E-7||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:LRO1(YNR008W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:MAM1(YER106W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MF(ALPHA)1(YPL187W)|FD-Score:3.68|P-value:1.14E-4||SGD DESC:Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor Gene:MRM1(YOR201C)|FD-Score:5|P-value:2.80E-7||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MRPL15(YLR312W-A)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL31(YKL138C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL38(YKL170W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:OPI1(YHL020C)|FD-Score:4.63|P-value:1.84E-6||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:OPT2(YPR194C)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PGM2(YMR105C)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Phosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress Gene:PHO86(YJL117W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PPM1(YDR435C)|FD-Score:-3.74|P-value:9.23E-5||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PTC3(YBL056W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication Gene:RGL1(YPL066W)|FD-Score:-3.8|P-value:7.18E-5||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RPL21B(YPL079W)|FD-Score:5.35|P-value:4.45E-8||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:5.51|P-value:1.80E-8||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RPS7B(YNL096C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RSA3(YLR221C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus Gene:RTC6(YPL183W-A)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SAS5(YOR213C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SET5(YHR207C)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SOD2(YHR008C)|FD-Score:9.17|P-value:2.28E-20||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SRT1(YMR101C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Cis-prenyltransferase involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase Gene:SWA2(YDR320C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TPM1(YNL079C)|FD-Score:-3.85|P-value:5.88E-5||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TPO1(YLL028W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane Gene:TRP1(YDR007W)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UBP16(YPL072W)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria Gene:UBP3(YER151C)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:VPS20(YMR077C)|FD-Score:-3.27|P-value:5.39E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS24(YKL041W)|FD-Score:3.09|P-value:9.89E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:YBR296C-A(YBR296C-A_p)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCF1(YDR135C)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YDR008C(YDR008C_d)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR114C(YDR114C_p)|FD-Score:4.87|P-value:5.71E-7||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YEL057C(YEL057C_p)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YGL108C(YGL108C_p)|FD-Score:3.86|P-value:5.63E-5||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YGL117W(YGL117W_p)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Putative protein of unknown function Gene:YGR051C(YGR051C_d)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene Gene:YIL134C-A(YIL134C-A_p)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YKL097C(YKL097C_d)|FD-Score:4.37|P-value:6.35E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species Gene:YKR045C(YKR045C_p)|FD-Score:-3.22|P-value:6.36E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YLR358C(YLR358C_p)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YML047W-A(YML047W-A_d)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML057C-A(YML057C-A_d)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W Gene:YMR253C(YMR253C_p)|FD-Score:4.4|P-value:5.37E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YMR310C(YMR310C_p)|FD-Score:-4.44|P-value:4.59E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNL184C(YNL184C_p)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL211C(YNL211C_p)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YNR005C(YNR005C_d)|FD-Score:3.88|P-value:5.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR293C-A(YOR293C-A_p)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPK1(YKL126W)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ZAP1(YJL056C)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:AAP1(YHR047C)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:AEP1(YMR064W)|FD-Score:5.53|P-value:1.59E-8||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AFI1(YOR129C)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Arf3p polarization-specific docking factor, required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p Gene:APL1(YJR005W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:ATP5(YDR298C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:AYR1(YIL124W)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BEM2(YER155C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:CCM1(YGR150C)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CDA2(YLR308W)|FD-Score:4.75|P-value:1.04E-6||SGD DESC:Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall Gene:CDH1(YGL003C)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:CEM1(YER061C)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:COG6(YNL041C)|FD-Score:6.19|P-value:3.04E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUP2(YGL166W)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:DCS1(YLR270W)|FD-Score:6.18|P-value:3.14E-10||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:FRE6(YLL051C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels Gene:GCN2(YDR283C)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GIS3(YLR094C)|FD-Score:3.91|P-value:4.57E-5||SGD DESC:Protein of unknown function Gene:HBS1(YKR084C)|FD-Score:4.28|P-value:9.23E-6||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HNT3(YOR258W)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HTL1(YCR020W-B)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:ISM1(YPL040C)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:JJJ3(YJR097W)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KAP122(YGL016W)|FD-Score:-3.79|P-value:7.49E-5||SGD DESC:Karyopherin beta, responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance Gene:LCB5(YLR260W)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LEU9(YOR108W)|FD-Score:4.95|P-value:3.76E-7||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:LRO1(YNR008W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:MAM1(YER106W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MF(ALPHA)1(YPL187W)|FD-Score:3.68|P-value:1.14E-4||SGD DESC:Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor Gene:MRM1(YOR201C)|FD-Score:5|P-value:2.80E-7||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MRPL15(YLR312W-A)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL31(YKL138C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL38(YKL170W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:OPI1(YHL020C)|FD-Score:4.63|P-value:1.84E-6||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:OPT2(YPR194C)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PGM2(YMR105C)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Phosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress Gene:PHO86(YJL117W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PPM1(YDR435C)|FD-Score:-3.74|P-value:9.23E-5||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PTC3(YBL056W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication Gene:RGL1(YPL066W)|FD-Score:-3.8|P-value:7.18E-5||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RPL21B(YPL079W)|FD-Score:5.35|P-value:4.45E-8||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:5.51|P-value:1.80E-8||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPS22A(YJL190C)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication Gene:RPS7B(YNL096C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RSA3(YLR221C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus Gene:RTC6(YPL183W-A)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SAS5(YOR213C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SET5(YHR207C)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SOD2(YHR008C)|FD-Score:9.17|P-value:2.28E-20||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SRT1(YMR101C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Cis-prenyltransferase involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase Gene:SWA2(YDR320C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TPM1(YNL079C)|FD-Score:-3.85|P-value:5.88E-5||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TPO1(YLL028W)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane Gene:TRP1(YDR007W)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UBP16(YPL072W)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Deubiquitinating enzyme anchored to the outer mitochondrial membrane, probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria Gene:UBP3(YER151C)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:VPS20(YMR077C)|FD-Score:-3.27|P-value:5.39E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS24(YKL041W)|FD-Score:3.09|P-value:9.89E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:YBR296C-A(YBR296C-A_p)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCF1(YDR135C)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YDR008C(YDR008C_d)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR114C(YDR114C_p)|FD-Score:4.87|P-value:5.71E-7||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YEL057C(YEL057C_p)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Protein of unknown function involved in telomere maintenance; target of UME6 regulation Gene:YGL108C(YGL108C_p)|FD-Score:3.86|P-value:5.63E-5||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YGL117W(YGL117W_p)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Putative protein of unknown function Gene:YGR051C(YGR051C_d)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene Gene:YIL134C-A(YIL134C-A_p)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YKL097C(YKL097C_d)|FD-Score:4.37|P-value:6.35E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species Gene:YKR045C(YKR045C_p)|FD-Score:-3.22|P-value:6.36E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YLR358C(YLR358C_p)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W Gene:YML047W-A(YML047W-A_d)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML057C-A(YML057C-A_d)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W Gene:YMR253C(YMR253C_p)|FD-Score:4.4|P-value:5.37E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YMR310C(YMR310C_p)|FD-Score:-4.44|P-value:4.59E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNL184C(YNL184C_p)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YNL211C(YNL211C_p)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YNR005C(YNR005C_d)|FD-Score:3.88|P-value:5.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR293C-A(YOR293C-A_p)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPK1(YKL126W)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ZAP1(YJL056C)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR449C3.571.80E-40.17UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YDL014W3.403.38E-40.03NOP1Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin
YDR232W3.373.72E-40.03HEM15-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p
YDR182W3.344.13E-40.04CDC1Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution
YPR094W3.304.84E-40.05RDS3Component of the SF3b subcomplex of the U2 snRNP, zinc cluster protein involved in pre-mRNA splicing and cycloheximide resistance
YPR144C3.255.74E-40.06NOC4Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits
YIL003W3.197.10E-40.13CFD1Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol
YER038C3.060.001110.05KRE29Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
YDL004W3.010.001300.15ATP16Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
YDL150W2.860.002110.03RPC53RNA polymerase III subunit C53
YDR228C2.830.002310.13PCF11mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
YOL144W2.700.003440.00NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YPR182W2.700.003470.00SMX3Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F
YIL026C2.700.003480.02IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YER127W2.680.003720.01LCP5Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR008C9.172.28E-20SOD2Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated
YNL041C6.193.04E-10COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YLR270W6.183.14E-10DCS1Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress
YMR064W5.531.59E-8AEP1Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase
YHL033C5.511.80E-8RPL8ARibosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication
YPL079W5.354.45E-8RPL21BRibosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication
YOR201C5.002.80E-7MRM1Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA
YOR108W4.953.76E-7LEU9Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication
YDR114C_p4.875.71E-7YDR114C_pPutative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium
YLR308W4.751.04E-6CDA2Chitin deacetylase, together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall
YJL056C4.741.06E-6ZAP1Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains
YDR135C4.651.69E-6YCF1Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR
YHL020C4.631.84E-6OPI1Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance
YMR253C_p4.405.37E-6YMR253C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene
YKL097C_d4.376.35E-6YKL097C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species

GO enrichment analysis for SGTC_979
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1142.37E-18SGTC_2232chlorophetanol 41.5 μMMiscellaneous159070.14TRP & mitochondrial translation
0.1125.98E-18SGTC_1742st038235 28.9 μMTimTec (Natural product derivative library)5641030.166667mitochondrial stress
0.1062.25E-16SGTC_100279-0212 42.6 μMChemDiv (Drug-like library)398510.12963TRP & mitochondrial translation
0.1063.71E-16SGTC_190279-0214 38.4 μMChemDiv (Drug-like library)35662740.12963TRP & mitochondrial translation
0.1054.82E-16SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.0666667TRP & mitochondrial translation
0.1024.18E-15SGTC_24885268135 45.7 μMMiscellaneous12703560.0625
0.0921.18E-12SGTC_465cgp-37157 41.0 μMICCB bioactive library26880.174603
0.0904.66E-12SGTC_5961155-0152 116.0 μMChemDiv (Drug-like library)2803130.103448TRP & mitochondrial translation
0.0898.18E-12SGTC_275trichlorophene 5.7 μMMiscellaneous626160.176471mitochondrial stress
0.0881.48E-11SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.135135
0.0872.53E-11SGTC_7234182-0001 115.0 μMChemDiv (Drug-like library)7448210.0769231
0.0863.08E-11SGTC_22957971645 200.0 μMChembridge (Fragment library)29782260.181818
0.0855.33E-11SGTC_1636st007707 68.9 μMTimTec (Natural product derivative library)4030510.1
0.0856.57E-11SGTC_1731273-0061 2.7 μMChemDiv (Drug-like library)28474140.0769231mitochondrial stress
0.0848.75E-11SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.0757576

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_12510490-5699265 μM0.5106383883818ChemDiv (Drug-like library)301.555783.66612
SGTC_22436634756200 μM0.456522885676Chembridge (Fragment library)234.079262.01512
SGTC_2890904672958.44 μM0.403846903329Chembridge (Drug-like library)270.114662.88412Golgi
SGTC_2866903424558.44 μM0.3684216462273Chembridge (Drug-like library)331.195984.41212
SGTC_7251497-096319 μM0.3396233549977ChemDiv (Drug-like library)356.117783.73215copper-dependent oxidative stress
SGTC_1240136-02177.12 μM0.338713976905ChemDiv (Drug-like library)476.223245.16417mitochondrial stress
SGTC_10154130-452582 μM0.3275861363237ChemDiv (Drug-like library)392.834983.8032360S ribosome export
SGTC_14780453-065635.7 μM0.3272735258496ChemDiv (Drug-like library)347.749762.81415
SGTC_21065357045167.29 μM0.327273668842Chembridge (Fragment library)281.13733.01412
SGTC_8261683-5028118 μM0.314815722377ChemDiv (Drug-like library)261.703583.26212