1497-1057

1-(4-bromophenyl)-N-(6-chloro-1,3-benzothiazol-2-yl)methanimine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_988
Screen concentration 103.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 3533044
SMILES C1=CC(=CC=C1C=NC2=NC3=C(S2)C=C(C=C3)Cl)Br
Standardized SMILES Clc1ccc2nc(N=Cc3ccc(Br)cc3)sc2c1
Molecular weight 351.6487
ALogP 5.46
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.67
% growth inhibition (Hom. pool) 4.32


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3533044
Download HIP data (tab-delimited text)  (excel)
Gene:CAB4(YGR277C)|FD-Score:4.96|P-value:3.45E-7|Clearance:0.77||SGD DESC:Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved Gene:DSN1(YIR010W)|FD-Score:-3.38|P-value:3.61E-4|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:KOG1(YHR186C)|FD-Score:3.15|P-value:8.11E-4|Clearance:0.05||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:LSG1(YGL099W)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MVD1(YNR043W)|FD-Score:3.1|P-value:9.52E-4|Clearance:0.06||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:NUP49(YGL172W)|FD-Score:4.2|P-value:1.34E-5|Clearance:0.74||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:TAD2(YJL035C)|FD-Score:5.4|P-value:3.27E-8|Clearance:0.77||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs Gene:UTP6(YDR449C)|FD-Score:5.06|P-value:2.14E-7|Clearance:0.77||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YRB1(YDR002W)|FD-Score:-3.4|P-value:3.42E-4|Clearance:0||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 Gene:ZIM17(YNL310C)|FD-Score:3.46|P-value:2.69E-4|Clearance:0.31||SGD DESC:Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix Gene:CAB4(YGR277C)|FD-Score:4.96|P-value:3.45E-7|Clearance:0.77||SGD DESC:Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved Gene:DSN1(YIR010W)|FD-Score:-3.38|P-value:3.61E-4|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:KOG1(YHR186C)|FD-Score:3.15|P-value:8.11E-4|Clearance:0.05||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:LSG1(YGL099W)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MVD1(YNR043W)|FD-Score:3.1|P-value:9.52E-4|Clearance:0.06||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:NUP49(YGL172W)|FD-Score:4.2|P-value:1.34E-5|Clearance:0.74||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:TAD2(YJL035C)|FD-Score:5.4|P-value:3.27E-8|Clearance:0.77||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs Gene:UTP6(YDR449C)|FD-Score:5.06|P-value:2.14E-7|Clearance:0.77||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YRB1(YDR002W)|FD-Score:-3.4|P-value:3.42E-4|Clearance:0||SGD DESC:Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 Gene:ZIM17(YNL310C)|FD-Score:3.46|P-value:2.69E-4|Clearance:0.31||SGD DESC:Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3533044
Download HOP data (tab-delimited text)  (excel)
Gene:AAP1(YHR047C)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:AIM13(YFR011C)|FD-Score:4.47|P-value:3.88E-6||SGD DESC:Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:ALD2(YMR170C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:ATG36(YJL185C)|FD-Score:4.91|P-value:4.49E-7||SGD DESC:Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene Gene:ATP4(YPL078C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex; phosphorylated Gene:BST1(YFL025C)|FD-Score:-3.35|P-value:4.08E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD6(YLR319C)|FD-Score:-3.22|P-value:6.48E-4||SGD DESC:Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate Gene:DAP2(YHR028C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:ECM15(YBL001C)|FD-Score:3.97|P-value:3.52E-5||SGD DESC:Non-essential protein of unknown function, likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis Gene:FIG1(YBR040W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating Gene:GRX2(YDR513W)|FD-Score:3.39|P-value:3.43E-4||SGD DESC:Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication Gene:HDA2(YDR295C)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance Gene:HHT2(YNL031C)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:HMI1(YOL095C)|FD-Score:5.48|P-value:2.08E-8||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HOM6(YJR139C)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HRT3(YLR097C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies Gene:JHD2(YJR119C)|FD-Score:3.86|P-value:5.79E-5||SGD DESC:JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation Gene:KKQ8(YKL168C)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:LRP1(YHR081W)|FD-Score:4.42|P-value:4.83E-6||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:LSB6(YJL100W)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MRP49(YKL167C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:NHP10(YDL002C)|FD-Score:5.31|P-value:5.37E-8||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NMA1(YLR328W)|FD-Score:-3.18|P-value:7.44E-4||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication Gene:NPP1(YCR026C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:NST1(YNL091W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:OYE2(YHR179W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PCD1(YLR151C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member Gene:PCT1(YGR202C)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity Gene:PDR11(YIL013C)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:RMD9(YGL107C)|FD-Score:-4.14|P-value:1.71E-5||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RPS24A(YER074W)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RRG8(YPR116W)|FD-Score:-3.47|P-value:2.65E-4||SGD DESC:Putative protein of unknown function, required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport Gene:SAC6(YDR129C)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDC1(YDR469W)|FD-Score:-4.36|P-value:6.53E-6||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SEH1(YGL100W)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SPC72(YAL047C)|FD-Score:3.84|P-value:6.23E-5||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SRB5(YGR104C)|FD-Score:-3.11|P-value:9.20E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SRX1(YKL086W)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress Gene:SUB1(YMR039C)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:VMA4(YOR332W)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:YAL065C(YAL065C_p)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YGR228W(YGR228W_d)|FD-Score:-3.13|P-value:8.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHR131C(YHR131C_p)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study Gene:YHR202W(YHR202W_p)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YIR021W-A(YIR021W-A_p)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJR096W(YJR096W)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YKR041W(YKR041W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking Gene:YNL011C(YNL011C_p)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Putative protein of unknown function; YNL011C is not an essential gene Gene:YOL159C(YOL159C)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Soluble protein of unknown function; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA Gene:YOR331C(YOR331C_d)|FD-Score:-3.75|P-value:8.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:YPR014C(YPR014C_d)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR039W(YPR039W_d)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:ZAP1(YJL056C)|FD-Score:-3.13|P-value:8.76E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:AAP1(YHR047C)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:AIM13(YFR011C)|FD-Score:4.47|P-value:3.88E-6||SGD DESC:Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:ALD2(YMR170C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:ATG36(YJL185C)|FD-Score:4.91|P-value:4.49E-7||SGD DESC:Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene Gene:ATP4(YPL078C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex; phosphorylated Gene:BST1(YFL025C)|FD-Score:-3.35|P-value:4.08E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD6(YLR319C)|FD-Score:-3.22|P-value:6.48E-4||SGD DESC:Actin- and formin-interacting protein; stimulates actin cable nucleation by recruiting actin monomers to Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate Gene:DAP2(YHR028C)|FD-Score:-3.59|P-value:1.67E-4||SGD DESC:Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p Gene:ECM15(YBL001C)|FD-Score:3.97|P-value:3.52E-5||SGD DESC:Non-essential protein of unknown function, likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis Gene:FIG1(YBR040W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating Gene:GRX2(YDR513W)|FD-Score:3.39|P-value:3.43E-4||SGD DESC:Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication Gene:HDA2(YDR295C)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance Gene:HHT2(YNL031C)|FD-Score:3.09|P-value:9.95E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:HMI1(YOL095C)|FD-Score:5.48|P-value:2.08E-8||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:HOM6(YJR139C)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HRT3(YLR097C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies Gene:JHD2(YJR119C)|FD-Score:3.86|P-value:5.79E-5||SGD DESC:JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation Gene:KKQ8(YKL168C)|FD-Score:-3.16|P-value:7.85E-4||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:LRP1(YHR081W)|FD-Score:4.42|P-value:4.83E-6||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:LSB6(YJL100W)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MRP49(YKL167C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:NHP10(YDL002C)|FD-Score:5.31|P-value:5.37E-8||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NMA1(YLR328W)|FD-Score:-3.18|P-value:7.44E-4||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication Gene:NPP1(YCR026C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:NST1(YNL091W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:OYE2(YHR179W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PCD1(YLR151C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member Gene:PCT1(YGR202C)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity Gene:PDR11(YIL013C)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:RMD9(YGL107C)|FD-Score:-4.14|P-value:1.71E-5||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RPS24A(YER074W)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RRG8(YPR116W)|FD-Score:-3.47|P-value:2.65E-4||SGD DESC:Putative protein of unknown function, required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport Gene:SAC6(YDR129C)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDC1(YDR469W)|FD-Score:-4.36|P-value:6.53E-6||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SEH1(YGL100W)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SPC72(YAL047C)|FD-Score:3.84|P-value:6.23E-5||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SRB5(YGR104C)|FD-Score:-3.11|P-value:9.20E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SRX1(YKL086W)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress Gene:SUB1(YMR039C)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:VMA4(YOR332W)|FD-Score:-3.54|P-value:2.03E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:YAL065C(YAL065C_p)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YGR228W(YGR228W_d)|FD-Score:-3.13|P-value:8.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHR131C(YHR131C_p)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study Gene:YHR202W(YHR202W_p)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YIR021W-A(YIR021W-A_p)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJR096W(YJR096W)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YKR041W(YKR041W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking Gene:YNL011C(YNL011C_p)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Putative protein of unknown function; YNL011C is not an essential gene Gene:YOL159C(YOL159C)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Soluble protein of unknown function; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA Gene:YOR331C(YOR331C_d)|FD-Score:-3.75|P-value:8.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:YPR014C(YPR014C_d)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR014C is not an essential gene Gene:YPR039W(YPR039W_d)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:ZAP1(YJL056C)|FD-Score:-3.13|P-value:8.76E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL035C5.403.27E-80.77TAD2Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs
YDR449C5.062.14E-70.77UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YGR277C4.963.45E-70.77CAB4Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved
YGL172W4.201.34E-50.74NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YNL310C3.462.69E-40.31ZIM17Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix
YHR186C3.158.11E-40.05KOG1Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
YNR043W3.109.52E-40.06MVD1Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer
YJL097W3.050.001150.07PHS1Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking
YMR047C2.980.001440.01NUP116FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p)
YLR195C2.970.001500.12NMT1N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
YDL150W2.840.002230.02RPC53RNA polymerase III subunit C53
YFR052W2.820.002370.01RPN12Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
YKL049C2.810.002480.01CSE4Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions
YGR119C2.800.002560.01NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YJL173C2.790.002630.01RFA3Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOL095C5.482.08E-8HMI1Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD
YDL002C5.315.37E-8NHP10Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair
YJL185C4.914.49E-7ATG36Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene
YKL086W4.671.48E-6SRX1Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress
YFR011C4.473.88E-6AIM13Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
YHR081W4.424.83E-6LRP1Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination
YNL011C_p4.261.01E-5YNL011C_pPutative protein of unknown function; YNL011C is not an essential gene
YJL100W4.241.10E-5LSB6Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization
YHR131C_p4.042.64E-5YHR131C_pPutative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study
YBL001C3.973.52E-5ECM15Non-essential protein of unknown function, likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis
YJR119C3.865.79E-5JHD2JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation
YAL047C3.846.23E-5SPC72Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization
YLR097C3.711.05E-4HRT3Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies
YBR040W3.701.08E-4FIG1Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating
YIL013C3.691.10E-4PDR11ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth

GO enrichment analysis for SGTC_988
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0808.03E-10SGTC_9871497-1013 404.0 μMChemDiv (Drug-like library)16764350.454545
0.0741.29E-8SGTC_31649100705 49.5 μMChembridge (Drug-like library)171742350.0540541
0.0543.53E-5SGTC_9711315-0384 26.7 μMChemDiv (Drug-like library)67691200.185185cell wall signaling
0.0526.01E-5SGTC_9861493-0483 84.0 μMChemDiv (Drug-like library)400790.0597015
0.0501.29E-4SGTC_492tmb-8 126.0 μMICCB bioactive library54940.0416667
0.0472.96E-4SGTC_28669034245 58.4 μMChembridge (Drug-like library)64622730.179104
0.0473.06E-4SGTC_900870-0863 4.6 μMChemDiv (Drug-like library)17381640.0694444
0.0473.40E-4SGTC_2550chrysin dimethyl ether 71.9 μMMicrosource (Natural product library)888810.0571429
0.0463.55E-4SGTC_9891501-0895 10.3 μMChemDiv (Drug-like library)28301120.164179
0.0463.84E-4SGTC_24415730946 39.1 μMMiscellaneous17742750.1375
0.0463.94E-4SGTC_24355706476 10.6 μMMiscellaneous57253750.241935
0.0464.31E-4SGTC_28799041815 71.4 μMChembridge (Drug-like library)49429710.0724638
0.0446.70E-4SGTC_1931273-0060 1.6 μMChemDiv (Drug-like library)28473930.0454545ergosterol biosynthesis
0.0446.88E-4SGTC_24635473429 200.0 μMMiscellaneous53994050.216667ERG2
0.0447.08E-4SGTC_3341502-1024 68.5 μMChemDiv (Drug-like library)32962160.0519481

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_9871497-1013404 μM0.4545451676435ChemDiv (Drug-like library)362.87526.58603
SGTC_12380333-028748.8 μM0.441867226ChemDiv (Drug-like library)184.6462.37313Golgi
SGTC_3371315-0038354 μM0.3181821626437ChemDiv (Drug-like library)423.357547.6103amide catabolism
SGTC_7981287-0182476 μM0.317461649755ChemDiv (Drug-like library)420.301825.78704
SGTC_710417-175047.81 μM0.31256811472ChemDiv (Drug-like library)364.848024.47714Golgi
SGTC_8830850-024524.6 μM0.31256823229ChemDiv (Drug-like library)409.299025.11814cell wall
SGTC_3241315-008819.6 μM0.3114756787421ChemDiv (Drug-like library)440.152284.60214RSC & ERG11
SGTC_6320781-23534.65 μM0.3076926811515ChemDiv (Drug-like library)443.744084.92914Golgi
SGTC_130199-008765 μM0.3035714589825ChemDiv (Drug-like library)354.229326.15902ERG2
SGTC_1320417-171525.81 μM0.3015876769098ChemDiv (Drug-like library)409.299024.56114RPP1 & pyrimidine depletion