3909-8734

N-(4-chlorophenyl)-6-methylthieno[2,3-d]pyrimidin-4-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_992
Screen concentration 162.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 4288319
SMILES CC1=CC2=C(N=CN=C2S1)NC3=CC=C(C=C3)Cl
Standardized SMILES Cc1cc2c(Nc3ccc(Cl)cc3)ncnc2s1
Molecular weight 275.7566
ALogP 4.01
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.28
% growth inhibition (Hom. pool) 7.69


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4288319
Download HIP data (tab-delimited text)  (excel)
Gene:ASA1(YPR085C)|FD-Score:-3.79|P-value:7.54E-5|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CDC1(YDR182W)|FD-Score:3.45|P-value:2.79E-4|Clearance:0.27||SGD DESC:Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution Gene:CMD1(YBR109C)|FD-Score:3.92|P-value:4.47E-5|Clearance:0.47||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:RPN3(YER021W)|FD-Score:4.27|P-value:9.95E-6|Clearance:0.47||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:SEN34(YAR008W)|FD-Score:3.13|P-value:8.81E-4|Clearance:0.16||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:SMC3(YJL074C)|FD-Score:3.18|P-value:7.39E-4|Clearance:0.05||SGD DESC:Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member Gene:SPC105(YGL093W)|FD-Score:-3.35|P-value:4.10E-4|Clearance:0||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SSS1(YDR086C)|FD-Score:-3.63|P-value:1.44E-4|Clearance:0||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:ASA1(YPR085C)|FD-Score:-3.79|P-value:7.54E-5|Clearance:0||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CDC1(YDR182W)|FD-Score:3.45|P-value:2.79E-4|Clearance:0.27||SGD DESC:Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution Gene:CMD1(YBR109C)|FD-Score:3.92|P-value:4.47E-5|Clearance:0.47||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:RPN3(YER021W)|FD-Score:4.27|P-value:9.95E-6|Clearance:0.47||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:SEN34(YAR008W)|FD-Score:3.13|P-value:8.81E-4|Clearance:0.16||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:SMC3(YJL074C)|FD-Score:3.18|P-value:7.39E-4|Clearance:0.05||SGD DESC:Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member Gene:SPC105(YGL093W)|FD-Score:-3.35|P-value:4.10E-4|Clearance:0||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SSS1(YDR086C)|FD-Score:-3.63|P-value:1.44E-4|Clearance:0||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4288319
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AEP1(YMR064W)|FD-Score:3.96|P-value:3.79E-5||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AFG3(YER017C)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AIM46(YHR199C)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARR2(YPR200C)|FD-Score:4.29|P-value:8.81E-6||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:ATG18(YFR021W)|FD-Score:-3.12|P-value:9.09E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATG23(YLR431C)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:CPR8(YNR028W)|FD-Score:-3.94|P-value:4.01E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway Gene:CSM2(YIL132C)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis Gene:CTF19(YPL018W)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:DAL81(YIR023W)|FD-Score:4.42|P-value:4.83E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCG1(YIR030C)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DIG1(YPL049C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DOG2(YHR043C)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:DSD1(YGL196W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:EHT1(YBR177C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:ERC1(YHR032W)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine Gene:ETT1(YOR051C)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough Gene:GDH3(YAL062W)|FD-Score:-3.97|P-value:3.60E-5||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GLO2(YDR272W)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GNT1(YOR320C)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:N-acetylglucosaminyltransferase capable of modification of N-linked glycans in the Golgi apparatus Gene:GPM2(YDL021W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:HEM14(YER014W)|FD-Score:8|P-value:6.16E-16||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HIT1(YJR055W)|FD-Score:4.53|P-value:2.94E-6||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HOM2(YDR158W)|FD-Score:-3.16|P-value:7.93E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:ICT1(YLR099C)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:INP2(YMR163C)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene Gene:IZH4(YOL101C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress Gene:JEN1(YKL217W)|FD-Score:-3.16|P-value:7.81E-4||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:MET32(YDR253C)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MGR2(YPL098C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MKT1(YNL085W)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MPA43(YNL249C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MRPS17(YMR188C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NAM2(YLR382C)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NVJ2(YPR091C)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments Gene:ODC2(YOR222W)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism Gene:PER33(YLR064W)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:PEX18(YHR160C)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PRM7(YDL039C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements Gene:RAS2(YNL098C)|FD-Score:5.05|P-value:2.17E-7||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RAX1(YOR301W)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily Gene:RCR1(YBR005W)|FD-Score:3.86|P-value:5.70E-5||SGD DESC:Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication Gene:RCY1(YJL204C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RPL2B(YIL018W)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPS1A(YLR441C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RPS7A(YOR096W)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:3.77|P-value:8.26E-5||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RRM3(YHR031C)|FD-Score:-3.93|P-value:4.27E-5||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:SLX1(YBR228W)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SPR6(YER115C)|FD-Score:3.76|P-value:8.65E-5||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SUV3(YPL029W)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SWH1(YAR042W)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction Gene:SWS2(YNL081C)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TEX1(YNL253W)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Protein involved in mRNA export, component of the transcription export (TREX) complex Gene:UMP1(YBR173C)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly Gene:YAP3(YHL009C)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YAR035C-A(YAR035C-A_p)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YER186C(YER186C_p)|FD-Score:4.42|P-value:4.83E-6||SGD DESC:Putative protein of unknown function Gene:YGR111W(YGR111W_p)|FD-Score:5.35|P-value:4.50E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YHR175W-A(YHR175W-A_p)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YHR210C(YHR210C_p)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YIL165C(YIL165C_p)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene Gene:YML018C(YML018C_p)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YMR087W(YMR087W)|FD-Score:6.11|P-value:4.88E-10||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YNL109W(YNL109W_d)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C Gene:YNR062C(YNR062C_p)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Putative membrane protein of unknown function Gene:YOR034C-A(YOR034C-A_p)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPR123C(YPR123C_d)|FD-Score:5.06|P-value:2.06E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YSP2(YDR326C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:ACB1(YGR037C)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AEP1(YMR064W)|FD-Score:3.96|P-value:3.79E-5||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AFG3(YER017C)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AIM46(YHR199C)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARR2(YPR200C)|FD-Score:4.29|P-value:8.81E-6||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:ATG18(YFR021W)|FD-Score:-3.12|P-value:9.09E-4||SGD DESC:Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein; relocalizes from vacuole to cytoplasm upon DNA replication stress; has 4 mammalian homologs WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood Gene:ATG23(YLR431C)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:CPR8(YNR028W)|FD-Score:-3.94|P-value:4.01E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway Gene:CSM2(YIL132C)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis Gene:CTF19(YPL018W)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Outer kinetochore protein, needed for accurate chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2 Gene:DAL81(YIR023W)|FD-Score:4.42|P-value:4.83E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCG1(YIR030C)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:DIG1(YPL049C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DOG2(YHR043C)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:DSD1(YGL196W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:EHT1(YBR177C)|FD-Score:3.25|P-value:5.87E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:ERC1(YHR032W)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine Gene:ETT1(YOR051C)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough Gene:GDH3(YAL062W)|FD-Score:-3.97|P-value:3.60E-5||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication Gene:GLO2(YDR272W)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GNT1(YOR320C)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:N-acetylglucosaminyltransferase capable of modification of N-linked glycans in the Golgi apparatus Gene:GPM2(YDL021W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:HEM14(YER014W)|FD-Score:8|P-value:6.16E-16||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HIT1(YJR055W)|FD-Score:4.53|P-value:2.94E-6||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HOM2(YDR158W)|FD-Score:-3.16|P-value:7.93E-4||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:ICT1(YLR099C)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:INP2(YMR163C)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene Gene:IZH4(YOL101C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress Gene:JEN1(YKL217W)|FD-Score:-3.16|P-value:7.81E-4||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:MET32(YDR253C)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MGR2(YPL098C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MKT1(YNL085W)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MPA43(YNL249C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MRPS17(YMR188C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NAM2(YLR382C)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NVJ2(YPR091C)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments Gene:ODC2(YOR222W)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism Gene:PER33(YLR064W)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress Gene:PEX18(YHR160C)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PRM7(YDL039C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements Gene:RAS2(YNL098C)|FD-Score:5.05|P-value:2.17E-7||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RAX1(YOR301W)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily Gene:RCR1(YBR005W)|FD-Score:3.86|P-value:5.70E-5||SGD DESC:Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication Gene:RCY1(YJL204C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RPL2B(YIL018W)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures Gene:RPS1A(YLR441C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:RPS7A(YOR096W)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:3.77|P-value:8.26E-5||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RRM3(YHR031C)|FD-Score:-3.93|P-value:4.27E-5||SGD DESC:DNA helicase involved in rDNA replication and Ty1 transposition; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p Gene:SLX1(YBR228W)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SPR6(YER115C)|FD-Score:3.76|P-value:8.65E-5||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SUV3(YPL029W)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SWH1(YAR042W)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction Gene:SWS2(YNL081C)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TEX1(YNL253W)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Protein involved in mRNA export, component of the transcription export (TREX) complex Gene:UMP1(YBR173C)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly Gene:YAP3(YHL009C)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YAR035C-A(YAR035C-A_p)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YER186C(YER186C_p)|FD-Score:4.42|P-value:4.83E-6||SGD DESC:Putative protein of unknown function Gene:YGR111W(YGR111W_p)|FD-Score:5.35|P-value:4.50E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YHR175W-A(YHR175W-A_p)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YHR210C(YHR210C_p)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YIL165C(YIL165C_p)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene Gene:YML018C(YML018C_p)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YMR087W(YMR087W)|FD-Score:6.11|P-value:4.88E-10||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YNL109W(YNL109W_d)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C Gene:YNR062C(YNR062C_p)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Putative membrane protein of unknown function Gene:YOR034C-A(YOR034C-A_p)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPR123C(YPR123C_d)|FD-Score:5.06|P-value:2.06E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YSP2(YDR326C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER021W4.279.95E-60.47RPN3Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
YBR109C3.924.47E-50.47CMD1Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin
YDR182W3.452.79E-40.27CDC1Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution
YJL074C3.187.39E-40.05SMC3Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member
YAR008W3.138.81E-40.16SEN34Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
YBR123C2.970.001480.06TFC1One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63
YER157W2.910.001810.02COG3Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YGR090W2.890.001950.00UTP22Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals
YBR002C2.880.001980.10RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YDR082W2.780.002720.02STN1Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping
YIL051C2.760.002930.01MMF1Mitochondrial protein required for transamination of isoleucine but not of valine or leucine; may regulate specificity of branched-chain transaminases Bat1p and Bat2p; interacts genetically with mitochondrial ribosomal protein genes
YDL147W2.750.003000.03RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YHR062C2.720.003300.05RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YHR188C2.670.003790.02GPI16Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog
YPL142C_d2.650.003990.06YPL142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER014W8.006.16E-16HEM14Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides
YMR087W6.114.88E-10YMR087WPutative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain
YGR111W_p5.354.50E-8YGR111W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
YPR123C_d5.062.06E-7YPR123C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR
YNL098C5.052.17E-7RAS2GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes
YDR253C4.701.28E-6MET32Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication
YJR055W4.532.94E-6HIT1Protein of unknown function, required for growth at high temperature
YER186C_p4.424.83E-6YER186C_pPutative protein of unknown function
YIR023W4.424.83E-6DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YPR200C4.298.81E-6ARR2Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p
YPL029W4.231.19E-5SUV3ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron
YLR064W4.191.39E-5PER33Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress
YBR173C4.141.74E-5UMP1Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly
YOR034C-A_p4.131.80E-5YOR034C-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YHL009C4.131.81E-5YAP3Basic leucine zipper (bZIP) transcription factor

GO enrichment analysis for SGTC_992
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0944.47E-13SGTC_9903985-0011 41.3 μMChemDiv (Drug-like library)53995650.0735294amide catabolism
0.0895.94E-12SGTC_486niguldipine 82.0 μMMiscellaneous12360.0631579amide catabolism
0.0831.92E-10SGTC_24605552655 174.2 μMMiscellaneous54019890.183333amide catabolism
0.0814.31E-10SGTC_3371315-0038 354.0 μMChemDiv (Drug-like library)16264370.180556amide catabolism
0.0764.84E-9SGTC_32259130819 49.5 μMChembridge (Drug-like library)45471210.132353amide catabolism
0.0741.07E-8SGTC_28459003024 43.0 μMChembridge (Drug-like library)29901730.121212amide catabolism
0.0723.21E-8SGTC_7003453-2283 142.0 μMChemDiv (Drug-like library)5656640.22807amide catabolism
0.0681.93E-7SGTC_30999120367 49.5 μMChembridge (Drug-like library)248249400.104478
0.0672.80E-7SGTC_9671306-0166 352.0 μMChemDiv (Drug-like library)44376070.142857
0.0663.95E-7SGTC_1759st043059 70.0 μMTimTec (Natural product derivative library)6694260.0909091amide catabolism
0.0655.21E-7SGTC_7441594-0068 283.0 μMChemDiv (Drug-like library)6683920.229167amide catabolism
0.0631.08E-6SGTC_30659113888 49.5 μMChembridge (Drug-like library)166483680.144928
0.0612.83E-6SGTC_2646retinol 96.8 μMMicrosource (Natural product library)4453540.0416667
0.0603.55E-6SGTC_2626ethyl orsellinate 100.0 μMMicrosource (Natural product library)756530.0847458
0.0603.96E-6SGTC_13211326-2144 215.0 μMChemDiv (Drug-like library)3513030NA

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_9933909-875762.7 μM0.6363644288324ChemDiv (Drug-like library)275.75664.01114
SGTC_3330914142471.43 μM0.42105327261020Chembridge (Drug-like library)308.400743.80324amide catabolism
SGTC_22587960471200 μM0.3653852974708Chembridge (Fragment library)298.834762.64715
SGTC_10124112-352218 μM0.3214293112398ChemDiv (Drug-like library)322.836243.81326
SGTC_5304358-171428 μM0.3157891564641ChemDiv (Drug-like library)293.362964.78914
SGTC_14023909-767335.9 μM0.3076925328014ChemDiv (Drug-like library)269.728883.98313
SGTC_13191306-015543.7 μM0.2962963122360ChemDiv (Drug-like library)252.657063.06724
SGTC_11293453-1384223 μM0.2931031084452ChemDiv (Drug-like library)358.189384.01115
SGTC_7593486-035889.3 μM0.290323875103ChemDiv (Drug-like library)315.371784.25814
SGTC_11891431-21197.28 μM0.283107275ChemDiv (Drug-like library)254.714244.76912