3931-2021

N-(4-ethoxyphenyl)-7-methyl-2,3-dihydro-1H-cyclopenta[b]quinolin-9-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_995
Screen concentration 34.6 μM
Source ChemDiv (Drug-like library)
PubChem CID 730143
SMILES CCOC1=CC=C(C=C1)NC2=C3CCCC3=NC4=C2C=C(C=C4)C
Standardized SMILES CCOc1ccc(Nc2c3CCCc3nc4ccc(C)cc24)cc1
Molecular weight 318.4122
ALogP 5.45
H-bond donor count 1
H-bond acceptor count 3
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.92
% growth inhibition (Hom. pool) 10.66


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 730143
Download HIP data (tab-delimited text)  (excel)
Gene:DED81(YHR019C)|FD-Score:3.45|P-value:2.79E-4|Clearance:0.03||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:GLC7(YER133W)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.1||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM15(YOR176W)|FD-Score:3.43|P-value:3.06E-4|Clearance:0.26||SGD DESC:Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway Gene:HYP2(YEL034W)|FD-Score:3.51|P-value:2.28E-4|Clearance:0.05||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:MED6(YHR058C)|FD-Score:-5.04|P-value:2.32E-7|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:PRP24(YMR268C)|FD-Score:-3.34|P-value:4.21E-4|Clearance:0||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RFC5(YBR087W)|FD-Score:3.17|P-value:7.69E-4|Clearance:0.11||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPP1(YHR062C)|FD-Score:4.75|P-value:1.03E-6|Clearance:0.24||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP3(YHR065C)|FD-Score:3.82|P-value:6.56E-5|Clearance:0.02||SGD DESC:Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity Gene:RSC58(YLR033W)|FD-Score:5.34|P-value:4.74E-8|Clearance:0.59||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SKP1(YDR328C)|FD-Score:3.8|P-value:7.13E-5|Clearance:0.19||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SLU7(YDR088C)|FD-Score:-3.56|P-value:1.84E-4|Clearance:0||SGD DESC:RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain Gene:SPP41(YDR464W)|FD-Score:4.03|P-value:2.74E-5|Clearance:0.21||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:SPT5(YML010W)|FD-Score:-3.44|P-value:2.88E-4|Clearance:0||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:STU2(YLR045C)|FD-Score:4.5|P-value:3.34E-6|Clearance:0.47||SGD DESC:Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p Gene:TIP20(YGL145W)|FD-Score:5.6|P-value:1.10E-8|Clearance:0.59||SGD DESC:Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p Gene:DED81(YHR019C)|FD-Score:3.45|P-value:2.79E-4|Clearance:0.03||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:GLC7(YER133W)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.1||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HEM15(YOR176W)|FD-Score:3.43|P-value:3.06E-4|Clearance:0.26||SGD DESC:Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway Gene:HYP2(YEL034W)|FD-Score:3.51|P-value:2.28E-4|Clearance:0.05||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:MED6(YHR058C)|FD-Score:-5.04|P-value:2.32E-7|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:PRP24(YMR268C)|FD-Score:-3.34|P-value:4.21E-4|Clearance:0||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RFC5(YBR087W)|FD-Score:3.17|P-value:7.69E-4|Clearance:0.11||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPP1(YHR062C)|FD-Score:4.75|P-value:1.03E-6|Clearance:0.24||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP3(YHR065C)|FD-Score:3.82|P-value:6.56E-5|Clearance:0.02||SGD DESC:Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity Gene:RSC58(YLR033W)|FD-Score:5.34|P-value:4.74E-8|Clearance:0.59||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SKP1(YDR328C)|FD-Score:3.8|P-value:7.13E-5|Clearance:0.19||SGD DESC:Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress Gene:SLU7(YDR088C)|FD-Score:-3.56|P-value:1.84E-4|Clearance:0||SGD DESC:RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain Gene:SPP41(YDR464W)|FD-Score:4.03|P-value:2.74E-5|Clearance:0.21||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:SPT5(YML010W)|FD-Score:-3.44|P-value:2.88E-4|Clearance:0||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:STU2(YLR045C)|FD-Score:4.5|P-value:3.34E-6|Clearance:0.47||SGD DESC:Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p Gene:TIP20(YGL145W)|FD-Score:5.6|P-value:1.10E-8|Clearance:0.59||SGD DESC:Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 730143
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AIM26(YKL037W)|FD-Score:4.63|P-value:1.87E-6||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:AMD2(YDR242W)|FD-Score:-3.24|P-value:5.87E-4||SGD DESC:Putative amidase Gene:APL4(YPR029C)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport Gene:ARL1(YBR164C)|FD-Score:-4.79|P-value:8.44E-7||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:AST2(YER101C)|FD-Score:3.87|P-value:5.52E-5||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:ATP12(YJL180C)|FD-Score:6.99|P-value:1.37E-12||SGD DESC:Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency Gene:ATP17(YDR377W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:ATP2(YJR121W)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:AUA1(YFL010W-A)|FD-Score:-3.76|P-value:8.45E-5||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:CAF16(YFL028C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator Gene:CDC73(YLR418C)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CEX1(YOR112W)|FD-Score:4.81|P-value:7.37E-7||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:CLB4(YLR210W)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation Gene:COX6(YHR051W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:DDI1(YER143W)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains Gene:EMC3(YKL207W)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 Gene:FLC2(YAL053W)|FD-Score:4|P-value:3.20E-5||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FSH1(YHR049W)|FD-Score:4.5|P-value:3.39E-6||SGD DESC:Putative serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:GLY1(YEL046C)|FD-Score:-3.9|P-value:4.88E-5||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GSP2(YOR185C)|FD-Score:3.25|P-value:5.67E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HEF3(YNL014W)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HEM14(YER014W)|FD-Score:-4.6|P-value:2.11E-6||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:IDP1(YDL066W)|FD-Score:4.36|P-value:6.41E-6||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IRS4(YKR019C)|FD-Score:-3.15|P-value:8.29E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IST3(YIR005W)|FD-Score:3.36|P-value:3.97E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:KCS1(YDR017C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:KSP1(YHR082C)|FD-Score:3.81|P-value:6.83E-5||SGD DESC:Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress Gene:LYS1(YIR034C)|FD-Score:4.37|P-value:6.35E-6||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MAL31(YBR298C)|FD-Score:9|P-value:1.09E-19||SGD DESC:Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C Gene:MBP1(YDL056W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MET32(YDR253C)|FD-Score:13.4|P-value:2.55E-41||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MIG3(YER028C)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MRPL15(YLR312W-A)|FD-Score:-6.01|P-value:9.05E-10||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL16(YBL038W)|FD-Score:4.3|P-value:8.68E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL23(YOR150W)|FD-Score:4.55|P-value:2.67E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL51(YPR100W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NNF2(YGR089W)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:NOP6(YDL213C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:OCA5(YHL029C_p)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OCT1(YKL134C)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:OPI9(YLR338W_d)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:RCR1(YBR005W)|FD-Score:-3.95|P-value:3.94E-5||SGD DESC:Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication Gene:REC104(YHR157W)|FD-Score:-4.32|P-value:7.89E-6||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RPL35B(YDL136W)|FD-Score:-3.3|P-value:4.85E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPN10(YHR200W)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RRT12(YCR045C)|FD-Score:4.28|P-value:9.46E-6||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RTT101(YJL047C)|FD-Score:-5.67|P-value:7.24E-9||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SHE4(YOR035C)|FD-Score:-3.33|P-value:4.38E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIN4(YNL236W)|FD-Score:-3.21|P-value:6.61E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SLA1(YBL007C)|FD-Score:7.99|P-value:6.73E-16||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLH1(YGR271W)|FD-Score:-3.86|P-value:5.68E-5||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SLM5(YCR024C)|FD-Score:4.79|P-value:8.38E-7||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SPT21(YMR179W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SSD1(YDR293C)|FD-Score:4.59|P-value:2.24E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUV3(YPL029W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TDA9(YML081W)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:TRE1(YPL176C)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:UIP5(YKR044W)|FD-Score:-4.86|P-value:5.97E-7||SGD DESC:Protein of unknown function that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates Gene:URK1(YNR012W)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP Gene:USV1(YPL230W)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Putative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in growth on non-fermentable carbon sources, response to salt stress and cell wall biosynthesis; USV1 has a paralog, RGM1, that arose from the whole genome duplication Gene:VMA22(YHR060W)|FD-Score:-5.18|P-value:1.10E-7||SGD DESC:Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) Gene:VPS61(YDR136C_d)|FD-Score:-4.85|P-value:6.11E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS68(YOL129W)|FD-Score:-6.07|P-value:6.30E-10||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YAL004W(YAL004W_d)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YBR012C(YBR012C_d)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YBR178W(YBR178W_d)|FD-Score:-4.99|P-value:3.00E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YDR210W(YDR210W_p)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR524W-C(YDR524W-C_p)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YER068C-A(YER068C-A_d)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL101W(YGL101W_p)|FD-Score:4.24|P-value:1.09E-5||SGD DESC:Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p Gene:YHC3(YJL059W)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YKL053W(YKL053W_d)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YLR031W(YLR031W_p)|FD-Score:4.43|P-value:4.72E-6||SGD DESC:Putative protein of unknown function Gene:YLR312C(YLR312C_p)|FD-Score:-4.41|P-value:5.21E-6||SGD DESC:Putative protein of unknown function Gene:YML034C-A(YML034C-A_d)|FD-Score:-3.82|P-value:6.70E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR087W(YMR087W)|FD-Score:3.82|P-value:6.63E-5||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YMR265C(YMR265C_p)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Putative protein of unknown function Gene:YMR316C-A(YMR316C-A_p)|FD-Score:3.8|P-value:7.38E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YPK1(YKL126W)|FD-Score:7.41|P-value:6.20E-14||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YTP1(YNL237W)|FD-Score:5.36|P-value:4.05E-8||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ACB1(YGR037C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AIM26(YKL037W)|FD-Score:4.63|P-value:1.87E-6||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:AMD2(YDR242W)|FD-Score:-3.24|P-value:5.87E-4||SGD DESC:Putative amidase Gene:APL4(YPR029C)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport Gene:ARL1(YBR164C)|FD-Score:-4.79|P-value:8.44E-7||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:AST2(YER101C)|FD-Score:3.87|P-value:5.52E-5||SGD DESC:Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication Gene:ATP12(YJL180C)|FD-Score:6.99|P-value:1.37E-12||SGD DESC:Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency Gene:ATP17(YDR377W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:ATP2(YJR121W)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:AUA1(YFL010W-A)|FD-Score:-3.76|P-value:8.45E-5||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:CAF16(YFL028C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator Gene:CDC73(YLR418C)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CEX1(YOR112W)|FD-Score:4.81|P-value:7.37E-7||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:CLB4(YLR210W)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation Gene:COX6(YHR051W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:DDI1(YER143W)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains Gene:EMC3(YKL207W)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 Gene:FLC2(YAL053W)|FD-Score:4|P-value:3.20E-5||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FSH1(YHR049W)|FD-Score:4.5|P-value:3.39E-6||SGD DESC:Putative serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:GLY1(YEL046C)|FD-Score:-3.9|P-value:4.88E-5||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GSP2(YOR185C)|FD-Score:3.25|P-value:5.67E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HEF3(YNL014W)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HEM14(YER014W)|FD-Score:-4.6|P-value:2.11E-6||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:IDP1(YDL066W)|FD-Score:4.36|P-value:6.41E-6||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IRS4(YKR019C)|FD-Score:-3.15|P-value:8.29E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IST3(YIR005W)|FD-Score:3.36|P-value:3.97E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:KCS1(YDR017C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:KSP1(YHR082C)|FD-Score:3.81|P-value:6.83E-5||SGD DESC:Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress Gene:LYS1(YIR034C)|FD-Score:4.37|P-value:6.35E-6||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MAL31(YBR298C)|FD-Score:9|P-value:1.09E-19||SGD DESC:Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C Gene:MBP1(YDL056W)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MET32(YDR253C)|FD-Score:13.4|P-value:2.55E-41||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MIG3(YER028C)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MRPL15(YLR312W-A)|FD-Score:-6.01|P-value:9.05E-10||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL16(YBL038W)|FD-Score:4.3|P-value:8.68E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL23(YOR150W)|FD-Score:4.55|P-value:2.67E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL51(YPR100W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NNF2(YGR089W)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:NOP6(YDL213C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:OCA5(YHL029C_p)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OCT1(YKL134C)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:OPI9(YLR338W_d)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:RCR1(YBR005W)|FD-Score:-3.95|P-value:3.94E-5||SGD DESC:Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR1 has a paralog, RCR2, that arose from the whole genome duplication Gene:REC104(YHR157W)|FD-Score:-4.32|P-value:7.89E-6||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RPL35B(YDL136W)|FD-Score:-3.3|P-value:4.85E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPN10(YHR200W)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RRT12(YCR045C)|FD-Score:4.28|P-value:9.46E-6||SGD DESC:Probable subtilisin-family protease with a role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores Gene:RTT101(YJL047C)|FD-Score:-5.67|P-value:7.24E-9||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SHE4(YOR035C)|FD-Score:-3.33|P-value:4.38E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIN4(YNL236W)|FD-Score:-3.21|P-value:6.61E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SLA1(YBL007C)|FD-Score:7.99|P-value:6.73E-16||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLH1(YGR271W)|FD-Score:-3.86|P-value:5.68E-5||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SLM5(YCR024C)|FD-Score:4.79|P-value:8.38E-7||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SPT21(YMR179W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SSD1(YDR293C)|FD-Score:4.59|P-value:2.24E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SUV3(YPL029W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TDA9(YML081W)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:TRE1(YPL176C)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:UIP5(YKR044W)|FD-Score:-4.86|P-value:5.97E-7||SGD DESC:Protein of unknown function that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates Gene:URK1(YNR012W)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP Gene:USV1(YPL230W)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Putative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in growth on non-fermentable carbon sources, response to salt stress and cell wall biosynthesis; USV1 has a paralog, RGM1, that arose from the whole genome duplication Gene:VMA22(YHR060W)|FD-Score:-5.18|P-value:1.10E-7||SGD DESC:Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER) Gene:VPS61(YDR136C_d)|FD-Score:-4.85|P-value:6.11E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS68(YOL129W)|FD-Score:-6.07|P-value:6.30E-10||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YAL004W(YAL004W_d)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YBR012C(YBR012C_d)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YBR178W(YBR178W_d)|FD-Score:-4.99|P-value:3.00E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YDR210W(YDR210W_p)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR524W-C(YDR524W-C_p)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YER068C-A(YER068C-A_d)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL101W(YGL101W_p)|FD-Score:4.24|P-value:1.09E-5||SGD DESC:Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p Gene:YHC3(YJL059W)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YKL053W(YKL053W_d)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 Gene:YLR031W(YLR031W_p)|FD-Score:4.43|P-value:4.72E-6||SGD DESC:Putative protein of unknown function Gene:YLR312C(YLR312C_p)|FD-Score:-4.41|P-value:5.21E-6||SGD DESC:Putative protein of unknown function Gene:YML034C-A(YML034C-A_d)|FD-Score:-3.82|P-value:6.70E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR087W(YMR087W)|FD-Score:3.82|P-value:6.63E-5||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YMR265C(YMR265C_p)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Putative protein of unknown function Gene:YMR316C-A(YMR316C-A_p)|FD-Score:3.8|P-value:7.38E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YPK1(YKL126W)|FD-Score:7.41|P-value:6.20E-14||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YTP1(YNL237W)|FD-Score:5.36|P-value:4.05E-8||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL145W5.601.10E-80.59TIP20Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p
YLR033W5.344.74E-80.59RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YHR062C4.751.03E-60.24RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YLR045C4.503.34E-60.47STU2Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p
YDR464W4.032.74E-50.21SPP41Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3
YHR065C3.826.56E-50.02RRP3Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity
YDR328C3.807.13E-50.20SKP1Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
YER133W3.611.54E-40.10GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YEL034W3.512.28E-40.05HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YHR019C3.452.79E-40.03DED81Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA
YOR176W3.433.06E-40.26HEM15Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway
YBR087W3.177.69E-40.11RFC5Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YLR105C3.050.001130.02SEN2Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease
YNL039W3.030.001210.03BDP1Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs
YGR114C_d3.010.001320.10YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR253C13.402.55E-41MET32Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication
YBR298C9.001.09E-19MAL31Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C
YBL007C7.996.73E-16SLA1Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains
YKL126W7.416.20E-14YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YJL180C6.991.37E-12ATP12Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency
YNL237W5.364.05E-8YTP1Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins
YOR112W4.817.37E-7CEX1Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p
YCR024C4.798.38E-7SLM5Mitochondrial asparaginyl-tRNA synthetase
YHL029C_p4.721.17E-6OCA5_pCytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts
YKL037W4.631.87E-6AIM26Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT
YDR293C4.592.24E-6SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YOR150W4.552.67E-6MRPL23Mitochondrial ribosomal protein of the large subunit
YHR049W4.503.39E-6FSH1Putative serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2
YLR031W_p4.434.72E-6YLR031W_pPutative protein of unknown function
YIR034C4.376.35E-6LYS1Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity

GO enrichment analysis for SGTC_995
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1284.03E-23SGTC_1627st003713 25.6 μMTimTec (Natural product derivative library)161950700.114583calcium & mitochondrial duress
0.1233.74E-21SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.0851064
0.1226.42E-21SGTC_2533agelasine 13.7 μMMicrosource (Natural product library)166677450.0673077ERG2
0.1218.51E-21SGTC_9943910-0338 138.0 μMChemDiv (Drug-like library)34794810.142857calcium & mitochondrial duress
0.1211.46E-20SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.137931
0.1188.10E-20SGTC_397arbidol 50.0 μMMiscellaneous1314100.0957447cell wall
0.1132.57E-18SGTC_9511171-0579 82.7 μMChemDiv (Drug-like library)16160500.075cell wall
0.1063.19E-16SGTC_275trichlorophene 5.7 μMMiscellaneous626160.0769231mitochondrial stress
0.1031.58E-15SGTC_28007887935 81.8 μMChembridge (Drug-like library)29610020.0609756RPP1 & pyrimidine depletion
0.1001.16E-14SGTC_13411486-1360 280.0 μMChemDiv (Drug-like library)39765470.147059
0.0979.64E-14SGTC_1573oleylamine 1.1 μMTimTec (Pure natural product library)53567890.0483871
0.0943.58E-13SGTC_33119131146 2.9 μMChembridge (Drug-like library)49004060.105882cell wall
0.0937.23E-13SGTC_502l-744,832 89.3 μMICCB bioactive library66103540.0769231calcium & mitochondrial duress
0.0905.31E-12SGTC_2720fluspirilene 36.9 μMMiscellaneous33960.0555556ERG2
0.0896.07E-12SGTC_2230bromochlorosalicylanilide 954.5 nMMiscellaneous772540.0972222

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_6023130-153338.1 μM0.596154720941ChemDiv (Drug-like library)274.359625.11612
SGTC_23266164455177.58 μM0.3606562888790Chembridge (Fragment library)260.761883.88722mitochondrial processes
SGTC_7734358-145744.6 μM0.3382351186886ChemDiv (Drug-like library)352.38383.73716
SGTC_3080911775449.47 μM0.3235299550191Chembridge (Drug-like library)306.321962.65716
SGTC_730327-032516.23 μM0.31034553298ChemDiv (Drug-like library)284.354445.38912DNA damage response
SGTC_2739tacrine100.86 μM0.2982461935Miscellaneous198.263662.7912heme requiring
SGTC_620180-04233.23 μM0.29411830102ChemDiv (Drug-like library)283.282022.8331560S ribosome export
SGTC_5304358-171428 μM0.2835821564641ChemDiv (Drug-like library)293.362964.78914
SGTC_6074491-098666.8 μM0.276923698981ChemDiv (Drug-like library)296.793985.48512heme requiring
SGTC_1994063-002441.6 μM0.274194306215ChemDiv (Drug-like library)311.783863.31414