4022-0066

6-methyl-5-nitro-2-(trifluoromethyl)chromen-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_999
Screen concentration 22.7 μM
Source ChemDiv (Drug-like library)
PubChem CID 740511
SMILES CC1=C(C2=C(C=C1)OC(=CC2=O)C(F)(F)F)[N+](=O)[O-]
Standardized SMILES Cc1ccc2OC(=CC(=O)c2c1[N+](=O)[O-])C(F)(F)F
Molecular weight 273.1649
ALogP 2.94
H-bond donor count 0
H-bond acceptor count 7
Response signature Golgi

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.55
% growth inhibition (Hom. pool) 4.68


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 740511
Download HIP data (tab-delimited text)  (excel)
Gene:BRN1(YBL097W)|FD-Score:4.59|P-value:2.26E-6|Clearance:0.09||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:DBP10(YDL031W)|FD-Score:4.6|P-value:2.15E-6|Clearance:0.01||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis Gene:DRE2(YKR071C)|FD-Score:3.59|P-value:1.64E-4|Clearance:0.06||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:ERG11(YHR007C)|FD-Score:7.68|P-value:8.10E-15|Clearance:0.02||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:FAS1(YKL182W)|FD-Score:3.9|P-value:4.76E-5|Clearance:0.15||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GFA1(YKL104C)|FD-Score:3.93|P-value:4.32E-5|Clearance:0.02||SGD DESC:Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication Gene:GWT1(YJL091C)|FD-Score:3.98|P-value:3.41E-5|Clearance:0.06||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:KOG1(YHR186C)|FD-Score:6.36|P-value:9.98E-11|Clearance:1.77||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:LSM4(YER112W)|FD-Score:3.3|P-value:4.83E-4|Clearance:0.03||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MAS1(YLR163C)|FD-Score:7.66|P-value:9.24E-15|Clearance:1.3||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MCM3(YEL032W)|FD-Score:4.34|P-value:7.26E-6|Clearance:0.15||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:MGE1(YOR232W)|FD-Score:3.22|P-value:6.31E-4|Clearance:0.23||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NEO1(YIL048W)|FD-Score:4.19|P-value:1.41E-5|Clearance:0.2||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:PFY1(YOR122C)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.01||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:RET2(YFR051C)|FD-Score:7.91|P-value:1.29E-15|Clearance:0.23||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RLP7(YNL002C)|FD-Score:3.71|P-value:1.05E-4|Clearance:0||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPL28(YGL103W)|FD-Score:3.27|P-value:5.40E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance Gene:RPT4(YOR259C)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.07||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RRP4(YHR069C)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.04||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SEC1(YDR164C)|FD-Score:-3.42|P-value:3.12E-4|Clearance:0||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SEC7(YDR170C)|FD-Score:47.4|P-value:0|Clearance:39.45||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SMC3(YJL074C)|FD-Score:-5.11|P-value:1.59E-7|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member Gene:SMD2(YLR275W)|FD-Score:3.75|P-value:8.89E-5|Clearance:0.04||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SSY5(YJL156C)|FD-Score:3.52|P-value:2.19E-4|Clearance:0.02||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:TAD3(YLR316C)|FD-Score:3.49|P-value:2.38E-4|Clearance:0.19||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs Gene:TFC3(YAL001C)|FD-Score:4.49|P-value:3.55E-6|Clearance:0.16||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:UTP20(YBL004W)|FD-Score:3.27|P-value:5.40E-4|Clearance:0.04||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:UTP8(YGR128C)|FD-Score:-4.08|P-value:2.29E-5|Clearance:0||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:BRN1(YBL097W)|FD-Score:4.59|P-value:2.26E-6|Clearance:0.09||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:DBP10(YDL031W)|FD-Score:4.6|P-value:2.15E-6|Clearance:0.01||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis Gene:DRE2(YKR071C)|FD-Score:3.59|P-value:1.64E-4|Clearance:0.06||SGD DESC:Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress Gene:ERG11(YHR007C)|FD-Score:7.68|P-value:8.10E-15|Clearance:0.02||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:FAS1(YKL182W)|FD-Score:3.9|P-value:4.76E-5|Clearance:0.15||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GFA1(YKL104C)|FD-Score:3.93|P-value:4.32E-5|Clearance:0.02||SGD DESC:Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication Gene:GWT1(YJL091C)|FD-Score:3.98|P-value:3.41E-5|Clearance:0.06||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:KOG1(YHR186C)|FD-Score:6.36|P-value:9.98E-11|Clearance:1.77||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:LSM4(YER112W)|FD-Score:3.3|P-value:4.83E-4|Clearance:0.03||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MAS1(YLR163C)|FD-Score:7.66|P-value:9.24E-15|Clearance:1.3||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MCM3(YEL032W)|FD-Score:4.34|P-value:7.26E-6|Clearance:0.15||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:MGE1(YOR232W)|FD-Score:3.22|P-value:6.31E-4|Clearance:0.23||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NEO1(YIL048W)|FD-Score:4.19|P-value:1.41E-5|Clearance:0.2||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:PFY1(YOR122C)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.01||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:RET2(YFR051C)|FD-Score:7.91|P-value:1.29E-15|Clearance:0.23||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RLP7(YNL002C)|FD-Score:3.71|P-value:1.05E-4|Clearance:0||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPL28(YGL103W)|FD-Score:3.27|P-value:5.40E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance Gene:RPT4(YOR259C)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.07||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RRP4(YHR069C)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.04||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:SEC1(YDR164C)|FD-Score:-3.42|P-value:3.12E-4|Clearance:0||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SEC7(YDR170C)|FD-Score:47.4|P-value:0|Clearance:39.45||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SMC3(YJL074C)|FD-Score:-5.11|P-value:1.59E-7|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member Gene:SMD2(YLR275W)|FD-Score:3.75|P-value:8.89E-5|Clearance:0.04||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SSY5(YJL156C)|FD-Score:3.52|P-value:2.19E-4|Clearance:0.02||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:TAD3(YLR316C)|FD-Score:3.49|P-value:2.38E-4|Clearance:0.19||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs Gene:TFC3(YAL001C)|FD-Score:4.49|P-value:3.55E-6|Clearance:0.16||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:UTP20(YBL004W)|FD-Score:3.27|P-value:5.40E-4|Clearance:0.04||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:UTP8(YGR128C)|FD-Score:-4.08|P-value:2.29E-5|Clearance:0||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 740511
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADE6(YGR061C)|FD-Score:-3.8|P-value:7.32E-5||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ARC1(YGL105W)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ARL1(YBR164C)|FD-Score:14.9|P-value:9.81E-51||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARL3(YPL051W)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARR2(YPR200C)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:ATP5(YDR298C)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BRE5(YNR051C)|FD-Score:7.67|P-value:8.89E-15||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:BSC1(YDL037C)|FD-Score:4.99|P-value:3.06E-7||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:BSC5(YNR069C)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:BTS1(YPL069C)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CHO2(YGR157W)|FD-Score:-3.44|P-value:2.94E-4||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:COG6(YNL041C)|FD-Score:8.98|P-value:1.38E-19||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:11|P-value:2.24E-28||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:8.35|P-value:3.43E-17||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CWH41(YGL027C)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DTR1(YBR180W)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:EMC3(YKL207W)|FD-Score:3.88|P-value:5.24E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 Gene:ERG4(YGL012W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERP5(YHR110W)|FD-Score:-5.81|P-value:3.08E-9||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FEN1(YCR034W)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:GAP1(YKR039W)|FD-Score:3.82|P-value:6.61E-5||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GDA1(YEL042W)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GEA2(YEL022W)|FD-Score:7.16|P-value:4.01E-13||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GEX2(YKR106W)|FD-Score:-4.03|P-value:2.78E-5||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GLO1(YML004C)|FD-Score:3.75|P-value:8.89E-5||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:GYP1(YOR070C)|FD-Score:7.01|P-value:1.18E-12||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:GYP6(YJL044C)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:GZF3(YJL110C)|FD-Score:4.41|P-value:5.09E-6||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HSP30(YCR021C)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase Gene:IES5(YER092W)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:IRC4(YDR540C)|FD-Score:5.52|P-value:1.71E-8||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRS4(YKR019C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LSM1(YJL124C)|FD-Score:14|P-value:4.83E-45||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LSM7(YNL147W)|FD-Score:5.63|P-value:8.98E-9||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MAC1(YMR021C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MFT1(YML062C)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MSG5(YNL053W)|FD-Score:4.29|P-value:9.01E-6||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:NBP2(YDR162C)|FD-Score:5.83|P-value:2.79E-9||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NEW1(YPL226W)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:OSH2(YDL019C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAN2(YGL094C)|FD-Score:4.49|P-value:3.54E-6||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PDC5(YLR134W)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PDX1(YGR193C)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core Gene:PEP12(YOR036W)|FD-Score:6.59|P-value:2.26E-11||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PHO13(YDL236W)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity Gene:PKH3(YDR466W)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PMT2(YAL023C)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PPT2(YPL148C)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRM10(YJL108C)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:PRO2(YOR323C)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PSR1(YLL010C)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:QDR3(YBR043C)|FD-Score:-4.28|P-value:9.31E-6||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:RCY1(YJL204C)|FD-Score:5.28|P-value:6.43E-8||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RMD9(YGL107C)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RPS28B(YLR264W)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:SAC7(YDR389W)|FD-Score:7.5|P-value:3.25E-14||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SWM1(YDR260C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation Gene:SWT21(YNL187W)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats Gene:SYS1(YJL004C)|FD-Score:14|P-value:1.02E-44||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TBS1(YBR150C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication Gene:TCB2(YNL087W)|FD-Score:3.95|P-value:3.95E-5||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:TDA5(YLR426W_p)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:THI72(YOR192C)|FD-Score:3.76|P-value:8.55E-5||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:TLG2(YOL018C)|FD-Score:3.82|P-value:6.67E-5||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPM1(YNL079C)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TRS33(YOR115C)|FD-Score:-4.51|P-value:3.21E-6||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TRS85(YDR108W)|FD-Score:6.56|P-value:2.77E-11||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:UBP3(YER151C)|FD-Score:4.81|P-value:7.36E-7||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:VHC1(YBR235W_p)|FD-Score:-3.89|P-value:5.06E-5||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:VPS1(YKR001C)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS45(YGL095C)|FD-Score:4.85|P-value:6.17E-7||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS63(YLR261C_d)|FD-Score:4.92|P-value:4.22E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS73(YGL104C)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:VPS74(YDR372C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:XBP1(YIL101C)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YAL044W-A(YAL044W-A_p)|FD-Score:-3.32|P-value:4.58E-4||SGD DESC:Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein Gene:YCR043C(YCR043C_p)|FD-Score:4.46|P-value:4.12E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YDR169C-A(YDR169C-A_p)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR455C(YDR455C_d)|FD-Score:6.83|P-value:4.12E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YEA6(YEL006W)|FD-Score:4.54|P-value:2.86E-6||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YER188W(YER188W_d)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p Gene:YGL188C-A(YGL188C-A_p)|FD-Score:3.82|P-value:6.54E-5||SGD DESC:Putative protein of unknown function Gene:YGR259C(YGR259C_d)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YHL045W(YHL045W_d)|FD-Score:3.97|P-value:3.58E-5||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YJL022W(YJL022W_d)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YJR061W(YJR061W_p)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication Gene:YKR040C(YKR040C_d)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W Gene:YOR022C(YOR022C_p)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR029W(YOR029W_d)|FD-Score:-3.17|P-value:7.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR039W(YPR039W_d)|FD-Score:3.76|P-value:8.48E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPR050C(YPR050C_d)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPT31(YER031C)|FD-Score:4.99|P-value:3.00E-7||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YUR1(YJL139C)|FD-Score:4.46|P-value:4.03E-6||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication Gene:ZRG8(YER033C)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency Gene:ACE2(YLR131C)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADE6(YGR061C)|FD-Score:-3.8|P-value:7.32E-5||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ARC1(YGL105W)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ARL1(YBR164C)|FD-Score:14.9|P-value:9.81E-51||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARL3(YPL051W)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ARR2(YPR200C)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:ATP5(YDR298C)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BRE5(YNR051C)|FD-Score:7.67|P-value:8.89E-15||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:BSC1(YDL037C)|FD-Score:4.99|P-value:3.06E-7||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:BSC5(YNR069C)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:BTS1(YPL069C)|FD-Score:4.04|P-value:2.63E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CHO2(YGR157W)|FD-Score:-3.44|P-value:2.94E-4||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:COG6(YNL041C)|FD-Score:8.98|P-value:1.38E-19||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:11|P-value:2.24E-28||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:8.35|P-value:3.43E-17||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CWH41(YGL027C)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress Gene:DTR1(YBR180W)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:EMC3(YKL207W)|FD-Score:3.88|P-value:5.24E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111 Gene:ERG4(YGL012W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERP5(YHR110W)|FD-Score:-5.81|P-value:3.08E-9||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FEN1(YCR034W)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:GAP1(YKR039W)|FD-Score:3.82|P-value:6.61E-5||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GDA1(YEL042W)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GEA2(YEL022W)|FD-Score:7.16|P-value:4.01E-13||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GEX2(YKR106W)|FD-Score:-4.03|P-value:2.78E-5||SGD DESC:Proton:glutathione antiporter localized to the vacuolar and plasma membranes; expressed at a very low level; almost identical to paralog Gex1p; potential role in resistance to oxidative stress and modulation of the PKA pathway Gene:GLO1(YML004C)|FD-Score:3.75|P-value:8.89E-5||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:GYP1(YOR070C)|FD-Score:7.01|P-value:1.18E-12||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:GYP6(YJL044C)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:GZF3(YJL110C)|FD-Score:4.41|P-value:5.09E-6||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HSP30(YCR021C)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase Gene:IES5(YER092W)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:IRC4(YDR540C)|FD-Score:5.52|P-value:1.71E-8||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRS4(YKR019C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LSM1(YJL124C)|FD-Score:14|P-value:4.83E-45||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LSM7(YNL147W)|FD-Score:5.63|P-value:8.98E-9||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MAC1(YMR021C)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MFT1(YML062C)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MSG5(YNL053W)|FD-Score:4.29|P-value:9.01E-6||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:NBP2(YDR162C)|FD-Score:5.83|P-value:2.79E-9||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NEW1(YPL226W)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:OSH2(YDL019C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PAN2(YGL094C)|FD-Score:4.49|P-value:3.54E-6||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PDC5(YLR134W)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PDX1(YGR193C)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core Gene:PEP12(YOR036W)|FD-Score:6.59|P-value:2.26E-11||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PHO13(YDL236W)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity Gene:PKH3(YDR466W)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PMT2(YAL023C)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PPT2(YPL148C)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRM10(YJL108C)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Pheromone-regulated protein, proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:PRO2(YOR323C)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PSR1(YLL010C)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:QDR3(YBR043C)|FD-Score:-4.28|P-value:9.31E-6||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:RCY1(YJL204C)|FD-Score:5.28|P-value:6.43E-8||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RMD9(YGL107C)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RPS28B(YLR264W)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:SAC7(YDR389W)|FD-Score:7.5|P-value:3.25E-14||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SWM1(YDR260C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation Gene:SWT21(YNL187W)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats Gene:SYS1(YJL004C)|FD-Score:14|P-value:1.02E-44||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TBS1(YBR150C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication Gene:TCB2(YNL087W)|FD-Score:3.95|P-value:3.95E-5||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:TDA5(YLR426W_p)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:THI72(YOR192C)|FD-Score:3.76|P-value:8.55E-5||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:TLG2(YOL018C)|FD-Score:3.82|P-value:6.67E-5||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TPM1(YNL079C)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TRS33(YOR115C)|FD-Score:-4.51|P-value:3.21E-6||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TRS85(YDR108W)|FD-Score:6.56|P-value:2.77E-11||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:UBP3(YER151C)|FD-Score:4.81|P-value:7.36E-7||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:VHC1(YBR235W_p)|FD-Score:-3.89|P-value:5.06E-5||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:VPS1(YKR001C)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS45(YGL095C)|FD-Score:4.85|P-value:6.17E-7||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS63(YLR261C_d)|FD-Score:4.92|P-value:4.22E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS73(YGL104C)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:VPS74(YDR372C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33 Gene:XBP1(YIL101C)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YAL044W-A(YAL044W-A_p)|FD-Score:-3.32|P-value:4.58E-4||SGD DESC:Putative protein of unknown function; similar to S. pombe uvi31 which is a putative DNA repair protein Gene:YCR043C(YCR043C_p)|FD-Score:4.46|P-value:4.12E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YDR169C-A(YDR169C-A_p)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR455C(YDR455C_d)|FD-Score:6.83|P-value:4.12E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YEA6(YEL006W)|FD-Score:4.54|P-value:2.86E-6||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YER188W(YER188W_d)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p Gene:YGL188C-A(YGL188C-A_p)|FD-Score:3.82|P-value:6.54E-5||SGD DESC:Putative protein of unknown function Gene:YGR259C(YGR259C_d)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YHL045W(YHL045W_d)|FD-Score:3.97|P-value:3.58E-5||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YJL022W(YJL022W_d)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YJR061W(YJR061W_p)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication Gene:YKR040C(YKR040C_d)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W Gene:YOR022C(YOR022C_p)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR029W(YOR029W_d)|FD-Score:-3.17|P-value:7.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR039W(YPR039W_d)|FD-Score:3.76|P-value:8.48E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPR050C(YPR050C_d)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YPT31(YER031C)|FD-Score:4.99|P-value:3.00E-7||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YUR1(YJL139C)|FD-Score:4.46|P-value:4.03E-6||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication Gene:ZRG8(YER033C)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR170C47.40039.50SEC7Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles
YFR051C7.911.29E-150.23RET2Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YHR007C7.688.10E-150.02ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YLR163C7.669.24E-151.30MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YHR186C6.369.98E-111.77KOG1Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
YDL031W4.602.15E-60.01DBP10Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis
YBL097W4.592.26E-60.09BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
YAL001C4.493.55E-60.15TFC3Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
YEL032W4.347.26E-60.15MCM3Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
YIL048W4.191.41E-50.20NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YJL091C3.983.41E-50.06GWT1Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors
YKL104C3.934.32E-50.02GFA1Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication
YKL182W3.904.76E-50.15FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YLR275W3.758.89E-50.04SMD2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
YNL002C3.711.05E-40.00RLP7Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR164C14.909.81E-51ARL1Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor
YJL124C14.004.83E-45LSM1Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress
YJL004C14.001.02E-44SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YGL005C11.002.24E-28COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YNL041C8.981.38E-19COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YML071C8.353.43E-17COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YNR051C7.678.89E-15BRE5Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A
YDR389W7.503.25E-14SAC7GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate
YEL022W7.164.01E-13GEA2Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
YOR070C7.011.18E-12GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YDR455C_d6.834.12E-12YDR455C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
YOR036W6.592.26E-11PEP12Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
YDR108W6.562.77E-11TRS85Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role
YDR162C5.832.79E-9NBP2Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM)
YNL147W5.638.98E-9LSM7Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress

GO enrichment analysis for SGTC_999
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.6640SGTC_22426630449 61.3 μMChembridge (Fragment library)6912490.236364Golgi
0.2845.52E-110SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.0454545Golgi
0.2362.54E-75SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.0344828Golgi
0.2277.21E-70SGTC_1581himbacine 57.9 μMTimTec (Pure natural product library)52818250.0641026Golgi
0.1939.70E-51SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.0806452Golgi
0.1859.81E-47SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.0365854Golgi
0.1757.09E-42SGTC_33259138760 55.2 μMChembridge (Drug-like library)438449590.0740741Golgi
0.1757.85E-42SGTC_31669102297 49.5 μMChembridge (Drug-like library)252370720.1Golgi
0.1702.59E-39SGTC_910417-1751 58.4 μMChemDiv (Drug-like library)67956240.037037Golgi
0.1672.87E-38SGTC_15956-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin 67.5 μMTimTec (Pure natural product library)6887880.164179
0.1602.56E-35SGTC_232clozapine 119.0 μMMiscellaneous28180.0540541NEO1
0.1563.94E-33SGTC_40960-0234 25.7 μMChemDiv (Drug-like library)68211120.0609756Golgi
0.1557.14E-33SGTC_21826325192 200.0 μMChembridge (Fragment library)7362480.0967742Golgi
0.1541.56E-32SGTC_30049076122 71.4 μMChembridge (Drug-like library)447825510.128571
0.1527.50E-32SGTC_6051315-0399 119.0 μMChemDiv (Drug-like library)240204610.0769231Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1732st03430851.82 μM0.333333830119TimTec (Natural product derivative library)277.531083.63602
SGTC_1727st0343099.9 μM0.290909870305TimTec (Natural product derivative library)293.530483.13403
SGTC_1723st0343072.55 μM0.288462867466TimTec (Natural product derivative library)297.949563.81402mitochondrial processes
SGTC_1722st0343043.65 μM0.284036148TimTec (Natural product derivative library)263.50453.1502mitochondrial processes
SGTC_1735st03431056.75 μM0.267857871153TimTec (Natural product derivative library)293.530483.13403Golgi
SGTC_15615-methoxyflavone52.9 μM0.24561494525TimTec (Pure natural product library)252.264683.1203
SGTC_15892',5'-dimethoxyflavone47.2 μM0.245614688667TimTec (Pure natural product library)282.290663.10304
SGTC_1703st02698348.3 μM0.2432431104023TimTec (Natural product derivative library)414.410125.02915
SGTC_1920st07572845.7 μM0.2394373400306TimTec (Natural product derivative library)314.292763.2615
SGTC_13591582-0056520 nM0.2380953661189ChemDiv (Drug-like library)304.688563.99416