YAL003W / EFB1

Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site

Zygosity: Heterozygous strain
fixedexpanded
Profile for YAL003W / EFB1

Click on Significant Values for Screen Details ID:SGTC_141|Compound:0443-0274|FD-Score:-3.98|P-value:9.31E-6 ID:SGTC_212|Compound:3807-4682|FD-Score:2.85|P-value:9.64E-4 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.45|P-value:8.61E-5 ID:SGTC_282|Compound:k297-0036|FD-Score:-5.10|P-value:2.07E-8 ID:SGTC_350|Compound:k064-0020|FD-Score:-4.12|P-value:4.89E-6 ID:SGTC_357|Compound:1325-0123|FD-Score:-4.61|P-value:3.61E-7 ID:SGTC_372|Compound:0155-0182|FD-Score:-4.47|P-value:7.93E-7 ID:SGTC_383|Compound:2324-0395|FD-Score:-4.20|P-value:3.21E-6 ID:SGTC_418|Compound:lividomycin a|FD-Score:-3.11|P-value:4.33E-4 ID:SGTC_420|Compound:mitomycin C|FD-Score:3.03|P-value:4.84E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:4.03|P-value:5.80E-6 ID:SGTC_438|Compound:sphingosine|FD-Score:-4.10|P-value:5.38E-6 ID:SGTC_470|Compound:aa-861|FD-Score:3.00|P-value:5.39E-4 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.44|P-value:9.24E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:-3.75|P-value:2.78E-5 ID:SGTC_796|Compound:1234-0020|FD-Score:-3.57|P-value:6.24E-5 ID:SGTC_806|Compound:3966-4455|FD-Score:3.16|P-value:2.85E-4 ID:SGTC_1010|Compound:4112-3296|FD-Score:3.34|P-value:1.40E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:3.92|P-value:9.93E-6 ID:SGTC_1469|Compound:k284-2957|FD-Score:-7.20|P-value:4.21E-15 ID:SGTC_1471|Compound:k673-0312|FD-Score:-3.12|P-value:4.02E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:5.09|P-value:1.60E-8 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.61|P-value:4.23E-5 ID:SGTC_2017|Compound:5132927|FD-Score:-3.07|P-value:4.99E-4 ID:SGTC_2019|Compound:4016004|FD-Score:3.29|P-value:1.70E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-2.95|P-value:7.92E-4 ID:SGTC_2160|Compound:5660466|FD-Score:3.30|P-value:1.67E-4 ID:SGTC_2200|Compound:6600604|FD-Score:4.28|P-value:1.63E-6 ID:SGTC_2268|Compound:7933382|FD-Score:3.00|P-value:5.49E-4 ID:SGTC_2282|Compound:7944268|FD-Score:2.90|P-value:7.77E-4 ID:SGTC_2401|Compound:5128683|FD-Score:4.64|P-value:2.29E-7 ID:SGTC_2417|Compound:cerulenin|FD-Score:-3.54|P-value:7.27E-5 ID:SGTC_2418|Compound:pentamidine|FD-Score:-8.08|P-value:1.54E-18 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-4.04|P-value:6.98E-6 ID:SGTC_2478|Compound:5763493|FD-Score:-3.50|P-value:8.51E-5 ID:SGTC_2479|Compound:5617353|FD-Score:5.36|P-value:2.87E-9 ID:SGTC_2628|Compound:lathosterol|FD-Score:-4.42|P-value:1.03E-6 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.01|P-value:5.29E-4 ID:SGTC_2715|Compound:st079500|FD-Score:4.44|P-value:6.96E-7 ID:SGTC_2718|Compound:lomofungin|FD-Score:4.24|P-value:1.98E-6 ID:SGTC_2725|Compound:haloperidol|FD-Score:3.40|P-value:1.08E-4 ID:SGTC_2739|Compound:tacrine|FD-Score:-4.02|P-value:7.89E-6 ID:SGTC_2759|Compound:disulfiram|FD-Score:-4.33|P-value:1.60E-6 ID:SGTC_2765|Compound:myclobutanil|FD-Score:-4.80|P-value:1.24E-7 ID:SGTC_2770|Compound:toltrazuril|FD-Score:3.14|P-value:3.11E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.85|P-value:1.73E-5 ID:SGTC_2791|Compound:5663188|FD-Score:4.10|P-value:4.15E-6 ID:SGTC_2814|Compound:7977482|FD-Score:3.29|P-value:1.69E-4 ID:SGTC_2832|Compound:7992762|FD-Score:-3.77|P-value:2.59E-5 ID:SGTC_2841|Compound:9004761|FD-Score:-3.05|P-value:5.35E-4 ID:SGTC_2847|Compound:9005661|FD-Score:3.63|P-value:3.96E-5 ID:SGTC_2905|Compound:7799065|FD-Score:-5.27|P-value:7.18E-9 ID:SGTC_2950|Compound:9072895|FD-Score:-5.13|P-value:1.76E-8 ID:SGTC_2965|Compound:9082772|FD-Score:-2.91|P-value:9.10E-4 ID:SGTC_2971|Compound:9092186|FD-Score:3.15|P-value:3.05E-4 ID:SGTC_3002|Compound:9075603|FD-Score:3.50|P-value:6.90E-5 ID:SGTC_3052|Compound:9092717|FD-Score:-2.91|P-value:9.14E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-3.33|P-value:1.73E-4 ID:SGTC_3112|Compound:9123419|FD-Score:3.60|P-value:4.44E-5 ID:SGTC_3140|Compound:9094243|FD-Score:3.52|P-value:6.28E-5 ID:SGTC_3208|Compound:9114115|FD-Score:3.32|P-value:1.53E-4 ID:SGTC_3210|Compound:9128157|FD-Score:4.13|P-value:3.48E-6 ID:SGTC_3223|Compound:9129970|FD-Score:3.15|P-value:3.01E-4 ID:SGTC_3224|Compound:9130643|FD-Score:2.97|P-value:6.07E-4 ID:SGTC_3250|Compound:9135583|FD-Score:-3.16|P-value:3.46E-4 ID:SGTC_3311|Compound:9131146|FD-Score:3.65|P-value:3.65E-5 ID:SGTC_3321|Compound:9137793|FD-Score:3.32|P-value:1.54E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.98|P-value:9.31E-6 ID:SGTC_212|Compound:3807-4682|FD-Score:2.85|P-value:9.64E-4 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.45|P-value:8.61E-5 ID:SGTC_282|Compound:k297-0036|FD-Score:-5.10|P-value:2.07E-8 ID:SGTC_350|Compound:k064-0020|FD-Score:-4.12|P-value:4.89E-6 ID:SGTC_357|Compound:1325-0123|FD-Score:-4.61|P-value:3.61E-7 ID:SGTC_372|Compound:0155-0182|FD-Score:-4.47|P-value:7.93E-7 ID:SGTC_383|Compound:2324-0395|FD-Score:-4.20|P-value:3.21E-6 ID:SGTC_418|Compound:lividomycin a|FD-Score:-3.11|P-value:4.33E-4 ID:SGTC_420|Compound:mitomycin C|FD-Score:3.03|P-value:4.84E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:4.03|P-value:5.80E-6 ID:SGTC_438|Compound:sphingosine|FD-Score:-4.10|P-value:5.38E-6 ID:SGTC_470|Compound:aa-861|FD-Score:3.00|P-value:5.39E-4 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.44|P-value:9.24E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:-3.75|P-value:2.78E-5 ID:SGTC_796|Compound:1234-0020|FD-Score:-3.57|P-value:6.24E-5 ID:SGTC_806|Compound:3966-4455|FD-Score:3.16|P-value:2.85E-4 ID:SGTC_1010|Compound:4112-3296|FD-Score:3.34|P-value:1.40E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:3.92|P-value:9.93E-6 ID:SGTC_1469|Compound:k284-2957|FD-Score:-7.20|P-value:4.21E-15 ID:SGTC_1471|Compound:k673-0312|FD-Score:-3.12|P-value:4.02E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:5.09|P-value:1.60E-8 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.61|P-value:4.23E-5 ID:SGTC_2017|Compound:5132927|FD-Score:-3.07|P-value:4.99E-4 ID:SGTC_2019|Compound:4016004|FD-Score:3.29|P-value:1.70E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-2.95|P-value:7.92E-4 ID:SGTC_2160|Compound:5660466|FD-Score:3.30|P-value:1.67E-4 ID:SGTC_2200|Compound:6600604|FD-Score:4.28|P-value:1.63E-6 ID:SGTC_2268|Compound:7933382|FD-Score:3.00|P-value:5.49E-4 ID:SGTC_2282|Compound:7944268|FD-Score:2.90|P-value:7.77E-4 ID:SGTC_2401|Compound:5128683|FD-Score:4.64|P-value:2.29E-7 ID:SGTC_2417|Compound:cerulenin|FD-Score:-3.54|P-value:7.27E-5 ID:SGTC_2418|Compound:pentamidine|FD-Score:-8.08|P-value:1.54E-18 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-4.04|P-value:6.98E-6 ID:SGTC_2478|Compound:5763493|FD-Score:-3.50|P-value:8.51E-5 ID:SGTC_2479|Compound:5617353|FD-Score:5.36|P-value:2.87E-9 ID:SGTC_2628|Compound:lathosterol|FD-Score:-4.42|P-value:1.03E-6 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.01|P-value:5.29E-4 ID:SGTC_2715|Compound:st079500|FD-Score:4.44|P-value:6.96E-7 ID:SGTC_2718|Compound:lomofungin|FD-Score:4.24|P-value:1.98E-6 ID:SGTC_2725|Compound:haloperidol|FD-Score:3.40|P-value:1.08E-4 ID:SGTC_2739|Compound:tacrine|FD-Score:-4.02|P-value:7.89E-6 ID:SGTC_2759|Compound:disulfiram|FD-Score:-4.33|P-value:1.60E-6 ID:SGTC_2765|Compound:myclobutanil|FD-Score:-4.80|P-value:1.24E-7 ID:SGTC_2770|Compound:toltrazuril|FD-Score:3.14|P-value:3.11E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-3.85|P-value:1.73E-5 ID:SGTC_2791|Compound:5663188|FD-Score:4.10|P-value:4.15E-6 ID:SGTC_2814|Compound:7977482|FD-Score:3.29|P-value:1.69E-4 ID:SGTC_2832|Compound:7992762|FD-Score:-3.77|P-value:2.59E-5 ID:SGTC_2841|Compound:9004761|FD-Score:-3.05|P-value:5.35E-4 ID:SGTC_2847|Compound:9005661|FD-Score:3.63|P-value:3.96E-5 ID:SGTC_2905|Compound:7799065|FD-Score:-5.27|P-value:7.18E-9 ID:SGTC_2950|Compound:9072895|FD-Score:-5.13|P-value:1.76E-8 ID:SGTC_2965|Compound:9082772|FD-Score:-2.91|P-value:9.10E-4 ID:SGTC_2971|Compound:9092186|FD-Score:3.15|P-value:3.05E-4 ID:SGTC_3002|Compound:9075603|FD-Score:3.50|P-value:6.90E-5 ID:SGTC_3052|Compound:9092717|FD-Score:-2.91|P-value:9.14E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-3.33|P-value:1.73E-4 ID:SGTC_3112|Compound:9123419|FD-Score:3.60|P-value:4.44E-5 ID:SGTC_3140|Compound:9094243|FD-Score:3.52|P-value:6.28E-5 ID:SGTC_3208|Compound:9114115|FD-Score:3.32|P-value:1.53E-4 ID:SGTC_3210|Compound:9128157|FD-Score:4.13|P-value:3.48E-6 ID:SGTC_3223|Compound:9129970|FD-Score:3.15|P-value:3.01E-4 ID:SGTC_3224|Compound:9130643|FD-Score:2.97|P-value:6.07E-4 ID:SGTC_3250|Compound:9135583|FD-Score:-3.16|P-value:3.46E-4 ID:SGTC_3311|Compound:9131146|FD-Score:3.65|P-value:3.65E-5 ID:SGTC_3321|Compound:9137793|FD-Score:3.32|P-value:1.54E-4

Top fitness defect scores for YAL003W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2479 5617353 5.36 43.9 µM 2.87E-9 1 4.05E-8 0.84
2 SGTC_1524 corynanthine 5.09 62.1 µM 1.60E-8 2 1.77E-7 0.46
3 SGTC_2401 5128683 4.64 200.0 µM 2.29E-7 5 1.72E-6 0.51
4 SGTC_2715 st079500 4.44 56.4 µM 6.96E-7 4 4.47E-6 0.75
5 SGTC_2200 6600604 4.28 21.9 µM 1.63E-6 tubulin folding & SWR complex 4 9.28E-6 0.16
6 SGTC_2718 lomofungin 4.24 53.0 µM 1.98E-6 mitochondrial processes 6 1.09E-5 0.14
7 SGTC_3210 9128157 4.13 49.5 µM 3.48E-6 12 1.78E-5 0.15
8 SGTC_2791 5663188 4.10 71.4 µM 4.15E-6 RPP1 & pyrimidine depletion 10 2.06E-5 0.28
9 SGTC_434 1-hexadecyl-2-methylglycero-3 PC 4.03 8.0 µM 5.80E-6 11 2.75E-5 0.26
10 SGTC_1014 4130-3005 3.92 69.3 µM 9.93E-6 7 4.36E-5 0.44
11 SGTC_3311 9131146 3.65 2.9 µM 3.65E-5 cell wall 23 1.33E-4 0.03
12 SGTC_2847 9005661 3.63 71.4 µM 3.96E-5 2 1.43E-4 0.32
13 SGTC_1525 8-methoxypsoralen 3.61 92.5 µM 4.23E-5 DNA damage response 17 1.51E-4 0.05
14 SGTC_3112 9123419 3.60 49.5 µM 4.44E-5 RPP1 & pyrimidine depletion 9 1.58E-4 0.20
15 SGTC_3140 9094243 3.52 49.5 µM 6.28E-5 9 2.13E-4 0.17
16 SGTC_3002 9075603 3.50 71.4 µM 6.90E-5 11 2.30E-4 0.05
17 SGTC_234 nsc-17383 3.45 10.7 µM 8.61E-5 ERG2 7 2.79E-4 0.07
18 SGTC_474 dl-dihydrosphingosine 3.44 5.0 µM 9.24E-5 13 2.96E-4 0.05
19 SGTC_2725 haloperidol 3.40 53.2 µM 1.08E-4 plasma membrane duress 25 3.38E-4 0.08
20 SGTC_1010 4112-3296 3.34 15.2 µM 1.40E-4 11 4.23E-4 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.171 2.69E-23 YAL032C PRP45 het Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
0.159 1.63E-20 YAL034W-A MTW1 het Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly
0.152 6.94E-19 YAR019C CDC15 het Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress
0.125 4.40E-13 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.123 8.31E-13 YNR017W TIM23 het Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel
0.123 8.76E-13 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.118 6.24E-12 YLR310C CDC25 het Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
0.111 1.24E-10 YAL001C TFC3 het Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding
0.109 2.31E-10 YBR294W SUL1 hom High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates
0.109 2.40E-10 YJL143W TIM17 het Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
0.107 5.76E-10 YNR046W TRM112 het Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress
0.106 8.13E-10 YBR155W CNS1 het TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion
0.103 1.84E-9 YGL207W SPT16 het Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome
0.102 3.03E-9 YOR060C SLD7 het Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria
0.102 3.06E-9 YDL174C DLD1 hom D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane