YAL012W / CYS3

Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YAL012W / CYS3

Click on Significant Values for Screen Details ID:SGTC_52|Compound:0329-0193|FD-Score:4.13|P-value:5.66E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.46|P-value:1.57E-5 ID:SGTC_107|Compound:0358-0022|FD-Score:4.59|P-value:1.50E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:-4.21|P-value:7.06E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:7.87|P-value:4.00E-10 ID:SGTC_134|Compound:0269-0018|FD-Score:6.53|P-value:1.60E-7 ID:SGTC_150|Compound:3970-0969|FD-Score:5.97|P-value:1.43E-6 ID:SGTC_173|Compound:1273-0061|FD-Score:4.72|P-value:1.04E-4 ID:SGTC_179|Compound:1273-0063|FD-Score:10.50|P-value:1.39E-16 ID:SGTC_184|Compound:1494-0393|FD-Score:7.67|P-value:1.01E-9 ID:SGTC_189|Compound:3970-0742|FD-Score:4.83|P-value:7.27E-5 ID:SGTC_190|Compound:3970-0790|FD-Score:4.43|P-value:2.44E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:5.59|P-value:5.84E-6 ID:SGTC_210|Compound:3551-0445|FD-Score:5.20|P-value:2.23E-5 ID:SGTC_212|Compound:3807-4682|FD-Score:4.79|P-value:8.34E-5 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:3.99|P-value:8.29E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:5.06|P-value:3.53E-5 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:5.61|P-value:5.44E-6 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:7.43|P-value:3.15E-9 ID:SGTC_279|Compound:1273-0055|FD-Score:5.97|P-value:1.42E-6 ID:SGTC_318|Compound:1488-0629|FD-Score:6.68|P-value:8.70E-8 ID:SGTC_328|Compound:k915-0120|FD-Score:4.39|P-value:2.75E-4 ID:SGTC_347|Compound:4534-1311|FD-Score:4.94|P-value:5.10E-5 ID:SGTC_377|Compound:3970-0964|FD-Score:4.08|P-value:6.46E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-4.27|P-value:5.86E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:11.70|P-value:5.55E-20 ID:SGTC_566|Compound:0958-0068|FD-Score:-4.85|P-value:1.13E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:4.64|P-value:1.30E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:4.99|P-value:4.35E-5 ID:SGTC_679|Compound:0422-0091|FD-Score:4.43|P-value:2.41E-4 ID:SGTC_731|Compound:3966-0296|FD-Score:3.99|P-value:8.22E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:6.72|P-value:7.11E-8 ID:SGTC_879|Compound:0831-1030|FD-Score:4.49|P-value:2.02E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:4.40|P-value:2.62E-4 ID:SGTC_897|Compound:1000-0139|FD-Score:5.85|P-value:2.27E-6 ID:SGTC_908|Compound:3448-7491|FD-Score:4.94|P-value:5.17E-5 ID:SGTC_918|Compound:glabranin|FD-Score:6.50|P-value:1.80E-7 ID:SGTC_950|Compound:1165-0508|FD-Score:5.92|P-value:1.73E-6 ID:SGTC_960|Compound:1222-0186|FD-Score:4.05|P-value:6.99E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:4.01|P-value:7.79E-4 ID:SGTC_984|Compound:1492-0255|FD-Score:4.60|P-value:1.49E-4 ID:SGTC_1041|Compound:0206-0046|FD-Score:6.77|P-value:5.96E-8 ID:SGTC_1101|Compound:ebselen|FD-Score:6.17|P-value:6.68E-7 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:-5.08|P-value:5.49E-5 ID:SGTC_1148|Compound:3909-7663|FD-Score:5.99|P-value:1.33E-6 ID:SGTC_1419|Compound:3993-2473|FD-Score:5.02|P-value:3.97E-5 ID:SGTC_1421|Compound:4029-0249|FD-Score:5.18|P-value:2.39E-5 ID:SGTC_1424|Compound:4048-1941|FD-Score:5.41|P-value:1.09E-5 ID:SGTC_1573|Compound:oleylamine|FD-Score:4.15|P-value:5.37E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:4.43|P-value:2.46E-4 ID:SGTC_1608|Compound:st001473|FD-Score:7.53|P-value:1.95E-9 ID:SGTC_1621|Compound:st003705|FD-Score:7.41|P-value:3.49E-9 ID:SGTC_1643|Compound:st009866|FD-Score:4.49|P-value:2.04E-4 ID:SGTC_1656|Compound:st012921|FD-Score:13.50|P-value:4.89E-26 ID:SGTC_1659|Compound:st012954|FD-Score:10.70|P-value:3.13E-17 ID:SGTC_1732|Compound:st034308|FD-Score:4.84|P-value:7.11E-5 ID:SGTC_1750|Compound:st081859|FD-Score:8.30|P-value:4.51E-11 ID:SGTC_1904|Compound:st060225|FD-Score:4.25|P-value:4.09E-4 ID:SGTC_1919|Compound:st067113|FD-Score:12.10|P-value:1.64E-21 ID:SGTC_1925|Compound:st057770|FD-Score:4.45|P-value:2.28E-4 ID:SGTC_1927|Compound:st060202|FD-Score:5.31|P-value:1.51E-5 ID:SGTC_2085|Compound:5307070|FD-Score:5.15|P-value:2.63E-5 ID:SGTC_2141|Compound:5621638|FD-Score:4.72|P-value:1.01E-4 ID:SGTC_2200|Compound:6600604|FD-Score:5.77|P-value:3.04E-6 ID:SGTC_2246|Compound:7293507|FD-Score:4.08|P-value:6.47E-4 ID:SGTC_2416|Compound:tofa|FD-Score:4.20|P-value:4.65E-4 ID:SGTC_2461|Compound:5755302|FD-Score:7.41|P-value:3.42E-9 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:4.29|P-value:3.59E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:6.23|P-value:5.36E-7 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:4.57|P-value:1.63E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:6.21|P-value:5.62E-7 ID:SGTC_2619|Compound:obliquin|FD-Score:4.06|P-value:6.82E-4 ID:SGTC_2671|Compound:danthron|FD-Score:5.88|P-value:1.98E-6 ID:SGTC_2900|Compound:5328528|FD-Score:-4.26|P-value:6.14E-4 ID:SGTC_2909|Compound:7941780|FD-Score:-5.46|P-value:1.56E-5 ID:SGTC_2910|Compound:7948405|FD-Score:7.31|P-value:5.41E-9 ID:SGTC_2968|Compound:9090663|FD-Score:-4.49|P-value:3.21E-4 ID:SGTC_3033|Compound:9090887|FD-Score:4.10|P-value:6.14E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:9.46|P-value:8.06E-14 ID:SGTC_3063|Compound:1327-0034|FD-Score:6.10|P-value:8.91E-7 ID:SGTC_3078|Compound:9116413|FD-Score:4.97|P-value:4.78E-5 ID:SGTC_3136|Compound:9095672|FD-Score:-5.38|P-value:2.04E-5 ID:SGTC_3143|Compound:9086658|FD-Score:-4.59|P-value:2.44E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-7.55|P-value:3.83E-9 ID:SGTC_3317|Compound:9138495|FD-Score:4.58|P-value:1.56E-4 ID:SGTC_52|Compound:0329-0193|FD-Score:4.13|P-value:5.66E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.46|P-value:1.57E-5 ID:SGTC_107|Compound:0358-0022|FD-Score:4.59|P-value:1.50E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:-4.21|P-value:7.06E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:7.87|P-value:4.00E-10 ID:SGTC_134|Compound:0269-0018|FD-Score:6.53|P-value:1.60E-7 ID:SGTC_150|Compound:3970-0969|FD-Score:5.97|P-value:1.43E-6 ID:SGTC_173|Compound:1273-0061|FD-Score:4.72|P-value:1.04E-4 ID:SGTC_179|Compound:1273-0063|FD-Score:10.50|P-value:1.39E-16 ID:SGTC_184|Compound:1494-0393|FD-Score:7.67|P-value:1.01E-9 ID:SGTC_189|Compound:3970-0742|FD-Score:4.83|P-value:7.27E-5 ID:SGTC_190|Compound:3970-0790|FD-Score:4.43|P-value:2.44E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:5.59|P-value:5.84E-6 ID:SGTC_210|Compound:3551-0445|FD-Score:5.20|P-value:2.23E-5 ID:SGTC_212|Compound:3807-4682|FD-Score:4.79|P-value:8.34E-5 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:3.99|P-value:8.29E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:5.06|P-value:3.53E-5 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:5.61|P-value:5.44E-6 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:7.43|P-value:3.15E-9 ID:SGTC_279|Compound:1273-0055|FD-Score:5.97|P-value:1.42E-6 ID:SGTC_318|Compound:1488-0629|FD-Score:6.68|P-value:8.70E-8 ID:SGTC_328|Compound:k915-0120|FD-Score:4.39|P-value:2.75E-4 ID:SGTC_347|Compound:4534-1311|FD-Score:4.94|P-value:5.10E-5 ID:SGTC_377|Compound:3970-0964|FD-Score:4.08|P-value:6.46E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-4.27|P-value:5.86E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:11.70|P-value:5.55E-20 ID:SGTC_566|Compound:0958-0068|FD-Score:-4.85|P-value:1.13E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:4.64|P-value:1.30E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:4.99|P-value:4.35E-5 ID:SGTC_679|Compound:0422-0091|FD-Score:4.43|P-value:2.41E-4 ID:SGTC_731|Compound:3966-0296|FD-Score:3.99|P-value:8.22E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:6.72|P-value:7.11E-8 ID:SGTC_879|Compound:0831-1030|FD-Score:4.49|P-value:2.02E-4 ID:SGTC_885|Compound:0865-0106|FD-Score:4.40|P-value:2.62E-4 ID:SGTC_897|Compound:1000-0139|FD-Score:5.85|P-value:2.27E-6 ID:SGTC_908|Compound:3448-7491|FD-Score:4.94|P-value:5.17E-5 ID:SGTC_918|Compound:glabranin|FD-Score:6.50|P-value:1.80E-7 ID:SGTC_950|Compound:1165-0508|FD-Score:5.92|P-value:1.73E-6 ID:SGTC_960|Compound:1222-0186|FD-Score:4.05|P-value:6.99E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:4.01|P-value:7.79E-4 ID:SGTC_984|Compound:1492-0255|FD-Score:4.60|P-value:1.49E-4 ID:SGTC_1041|Compound:0206-0046|FD-Score:6.77|P-value:5.96E-8 ID:SGTC_1101|Compound:ebselen|FD-Score:6.17|P-value:6.68E-7 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:-5.08|P-value:5.49E-5 ID:SGTC_1148|Compound:3909-7663|FD-Score:5.99|P-value:1.33E-6 ID:SGTC_1419|Compound:3993-2473|FD-Score:5.02|P-value:3.97E-5 ID:SGTC_1421|Compound:4029-0249|FD-Score:5.18|P-value:2.39E-5 ID:SGTC_1424|Compound:4048-1941|FD-Score:5.41|P-value:1.09E-5 ID:SGTC_1573|Compound:oleylamine|FD-Score:4.15|P-value:5.37E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:4.43|P-value:2.46E-4 ID:SGTC_1608|Compound:st001473|FD-Score:7.53|P-value:1.95E-9 ID:SGTC_1621|Compound:st003705|FD-Score:7.41|P-value:3.49E-9 ID:SGTC_1643|Compound:st009866|FD-Score:4.49|P-value:2.04E-4 ID:SGTC_1656|Compound:st012921|FD-Score:13.50|P-value:4.89E-26 ID:SGTC_1659|Compound:st012954|FD-Score:10.70|P-value:3.13E-17 ID:SGTC_1732|Compound:st034308|FD-Score:4.84|P-value:7.11E-5 ID:SGTC_1750|Compound:st081859|FD-Score:8.30|P-value:4.51E-11 ID:SGTC_1904|Compound:st060225|FD-Score:4.25|P-value:4.09E-4 ID:SGTC_1919|Compound:st067113|FD-Score:12.10|P-value:1.64E-21 ID:SGTC_1925|Compound:st057770|FD-Score:4.45|P-value:2.28E-4 ID:SGTC_1927|Compound:st060202|FD-Score:5.31|P-value:1.51E-5 ID:SGTC_2085|Compound:5307070|FD-Score:5.15|P-value:2.63E-5 ID:SGTC_2141|Compound:5621638|FD-Score:4.72|P-value:1.01E-4 ID:SGTC_2200|Compound:6600604|FD-Score:5.77|P-value:3.04E-6 ID:SGTC_2246|Compound:7293507|FD-Score:4.08|P-value:6.47E-4 ID:SGTC_2416|Compound:tofa|FD-Score:4.20|P-value:4.65E-4 ID:SGTC_2461|Compound:5755302|FD-Score:7.41|P-value:3.42E-9 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:4.29|P-value:3.59E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:6.23|P-value:5.36E-7 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:4.57|P-value:1.63E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:6.21|P-value:5.62E-7 ID:SGTC_2619|Compound:obliquin|FD-Score:4.06|P-value:6.82E-4 ID:SGTC_2671|Compound:danthron|FD-Score:5.88|P-value:1.98E-6 ID:SGTC_2900|Compound:5328528|FD-Score:-4.26|P-value:6.14E-4 ID:SGTC_2909|Compound:7941780|FD-Score:-5.46|P-value:1.56E-5 ID:SGTC_2910|Compound:7948405|FD-Score:7.31|P-value:5.41E-9 ID:SGTC_2968|Compound:9090663|FD-Score:-4.49|P-value:3.21E-4 ID:SGTC_3033|Compound:9090887|FD-Score:4.10|P-value:6.14E-4 ID:SGTC_3059|Compound:1327-0034|FD-Score:9.46|P-value:8.06E-14 ID:SGTC_3063|Compound:1327-0034|FD-Score:6.10|P-value:8.91E-7 ID:SGTC_3078|Compound:9116413|FD-Score:4.97|P-value:4.78E-5 ID:SGTC_3136|Compound:9095672|FD-Score:-5.38|P-value:2.04E-5 ID:SGTC_3143|Compound:9086658|FD-Score:-4.59|P-value:2.44E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-7.55|P-value:3.83E-9 ID:SGTC_3317|Compound:9138495|FD-Score:4.58|P-value:1.56E-4

Top fitness defect scores for YAL012W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1656 st012921 13.50 11.0 µM 4.89E-26 6 1.02E-41
2 SGTC_1919 st067113 12.10 52.3 µM 1.64E-21 TSC3-RPN4 2 3.15E-34
3 SGTC_513 4245-1575 11.70 41.7 µM 5.55E-20 unfolded protein response 10 1.07E-31
4 SGTC_1659 st012954 10.70 42.1 µM 3.13E-17 1 3.72E-27
5 SGTC_179 1273-0063 10.50 6.8 µM 1.39E-16 1 4.35E-26
6 SGTC_3059 1327-0034 9.46 3.0 µM 8.06E-14 13 1.56E-21
7 SGTC_1750 st081859 8.30 2.4 µM 4.51E-11 endomembrane recycling 65 5.14E-17
8 SGTC_129 0088-0017 7.87 176.6 µM 4.00E-10 17 1.84E-15
9 SGTC_184 1494-0393 7.67 24.7 µM 1.01E-9 10 8.42E-15
10 SGTC_1608 st001473 7.53 40.5 µM 1.95E-9 8 2.47E-14
11 SGTC_243 5hr heat shock (37°C) + splitomicin 7.43 1.0 µM 3.15E-9 heat shock/prefoldin 12 5.41E-14
12 SGTC_2461 5755302 7.41 26.1 µM 3.42E-9 6 6.21E-14
13 SGTC_1621 st003705 7.41 32.4 µM 3.49E-9 calcium & mitochondrial duress 4 6.41E-14
14 SGTC_2910 7948405 7.31 26.0 µM 5.41E-9 plasma membrane duress 6 1.31E-13
15 SGTC_1041 0206-0046 6.77 107.0 µM 5.96E-8 10 6.62E-12
16 SGTC_852 0418-0143 6.72 439.0 µM 7.11E-8 calcium & mitochondrial duress 19 8.83E-12
17 SGTC_318 1488-0629 6.68 67.2 µM 8.70E-8 copper-dependent oxidative stress 10 1.23E-11
18 SGTC_134 0269-0018 6.53 6.9 µM 1.60E-7 74 3.33E-11
19 SGTC_918 glabranin 6.50 770.7 nM 1.80E-7 3 4.00E-11
20 SGTC_2540 epiandrosterone 6.23 100.0 µM 5.36E-7 20 2.37E-10

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.278 3.96E-60 YGR155W CYS4 hom Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria
0.230 2.41E-41 YBR058C-A TSC3 hom Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
0.173 6.14E-24 YLR027C AAT2 hom Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
0.149 3.81E-18 YER086W ILV1 hom Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation
0.148 9.77E-18 YLR087C CSF1 hom Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.144 7.39E-17 YEL062W NPR2 hom Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline
0.142 1.44E-16 YLR200W YKE2 hom Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
0.141 2.09E-16 YDR069C DOA4 hom Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.139 7.12E-16 YAL024C LTE1 hom Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
0.136 3.56E-15 YPR060C ARO7 hom Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
0.129 5.84E-14 YOR349W CIN1 hom Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
0.129 6.68E-14 YGL167C PMR1 hom High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease
0.128 1.10E-13 YLR417W VPS36 hom Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
0.125 3.71E-13 YHR179W OYE2 hom Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress
0.124 6.50E-13 YJR073C OPI3 hom Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis