YAL023C / PMT2

Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YAL023C / PMT2

Click on Significant Values for Screen Details ID:SGTC_44|Compound:0929-0055|FD-Score:4.97|P-value:5.65E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:3.94|P-value:2.55E-4 ID:SGTC_101|Compound:0490-5701|FD-Score:-3.64|P-value:5.53E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-6.39|P-value:5.89E-9 ID:SGTC_112|Compound:phenothiazine|FD-Score:-4.28|P-value:6.43E-5 ID:SGTC_117|Compound:lucigenin|FD-Score:4.93|P-value:6.52E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:3.81|P-value:3.85E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:3.81|P-value:3.89E-4 ID:SGTC_147|Compound:4466-0538|FD-Score:-3.94|P-value:2.13E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.13|P-value:1.33E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:3.51|P-value:9.71E-4 ID:SGTC_452|Compound:diindolylmethane|FD-Score:4.97|P-value:5.61E-6 ID:SGTC_728|Compound:3030-5151|FD-Score:3.67|P-value:6.08E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:3.54|P-value:8.89E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:5.95|P-value:6.88E-8 ID:SGTC_1015|Compound:4130-4525|FD-Score:4.90|P-value:7.36E-6 ID:SGTC_1188|Compound:1403-0014|FD-Score:3.55|P-value:8.68E-4 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:3.68|P-value:5.83E-4 ID:SGTC_2165|Compound:5727197|FD-Score:3.83|P-value:3.65E-4 ID:SGTC_2286|Compound:7945697|FD-Score:4.28|P-value:7.94E-5 ID:SGTC_2289|Compound:7946404|FD-Score:5.60|P-value:3.65E-7 ID:SGTC_2296|Compound:7971656|FD-Score:4.55|P-value:2.92E-5 ID:SGTC_2393|Compound:9031399|FD-Score:5.14|P-value:2.72E-6 ID:SGTC_2447|Compound:5741742|FD-Score:4.11|P-value:1.43E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:-4.68|P-value:1.41E-5 ID:SGTC_2581|Compound:avocatin a|FD-Score:4.19|P-value:1.08E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.51|P-value:9.73E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:5.48|P-value:6.32E-7 ID:SGTC_2589|Compound:lobaric acid|FD-Score:5.32|P-value:1.30E-6 ID:SGTC_2691|Compound:st077356|FD-Score:3.61|P-value:7.18E-4 ID:SGTC_2699|Compound:st077704|FD-Score:3.79|P-value:4.07E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.53|P-value:9.22E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:-3.48|P-value:9.17E-4 ID:SGTC_2788|Compound:5282734|FD-Score:-3.51|P-value:8.43E-4 ID:SGTC_2802|Compound:7925815|FD-Score:4.04|P-value:1.78E-4 ID:SGTC_2855|Compound:9016525|FD-Score:3.53|P-value:9.14E-4 ID:SGTC_2905|Compound:7799065|FD-Score:-4.76|P-value:1.04E-5 ID:SGTC_2959|Compound:9079155|FD-Score:3.78|P-value:4.21E-4 ID:SGTC_2981|Compound:9013952|FD-Score:4.66|P-value:1.95E-5 ID:SGTC_3012|Compound:9080433|FD-Score:3.64|P-value:6.67E-4 ID:SGTC_3038|Compound:9091290|FD-Score:-3.99|P-value:1.81E-4 ID:SGTC_3132|Compound:9126706|FD-Score:4.78|P-value:1.20E-5 ID:SGTC_3149|Compound:9097855|FD-Score:4.05|P-value:1.75E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:4.97|P-value:5.65E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:3.94|P-value:2.55E-4 ID:SGTC_101|Compound:0490-5701|FD-Score:-3.64|P-value:5.53E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:-6.39|P-value:5.89E-9 ID:SGTC_112|Compound:phenothiazine|FD-Score:-4.28|P-value:6.43E-5 ID:SGTC_117|Compound:lucigenin|FD-Score:4.93|P-value:6.52E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:3.81|P-value:3.85E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:3.81|P-value:3.89E-4 ID:SGTC_147|Compound:4466-0538|FD-Score:-3.94|P-value:2.13E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.13|P-value:1.33E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:3.51|P-value:9.71E-4 ID:SGTC_452|Compound:diindolylmethane|FD-Score:4.97|P-value:5.61E-6 ID:SGTC_728|Compound:3030-5151|FD-Score:3.67|P-value:6.08E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:3.54|P-value:8.89E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:5.95|P-value:6.88E-8 ID:SGTC_1015|Compound:4130-4525|FD-Score:4.90|P-value:7.36E-6 ID:SGTC_1188|Compound:1403-0014|FD-Score:3.55|P-value:8.68E-4 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:3.68|P-value:5.83E-4 ID:SGTC_2165|Compound:5727197|FD-Score:3.83|P-value:3.65E-4 ID:SGTC_2286|Compound:7945697|FD-Score:4.28|P-value:7.94E-5 ID:SGTC_2289|Compound:7946404|FD-Score:5.60|P-value:3.65E-7 ID:SGTC_2296|Compound:7971656|FD-Score:4.55|P-value:2.92E-5 ID:SGTC_2393|Compound:9031399|FD-Score:5.14|P-value:2.72E-6 ID:SGTC_2447|Compound:5741742|FD-Score:4.11|P-value:1.43E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:-4.68|P-value:1.41E-5 ID:SGTC_2581|Compound:avocatin a|FD-Score:4.19|P-value:1.08E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.51|P-value:9.73E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:5.48|P-value:6.32E-7 ID:SGTC_2589|Compound:lobaric acid|FD-Score:5.32|P-value:1.30E-6 ID:SGTC_2691|Compound:st077356|FD-Score:3.61|P-value:7.18E-4 ID:SGTC_2699|Compound:st077704|FD-Score:3.79|P-value:4.07E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.53|P-value:9.22E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:-3.48|P-value:9.17E-4 ID:SGTC_2788|Compound:5282734|FD-Score:-3.51|P-value:8.43E-4 ID:SGTC_2802|Compound:7925815|FD-Score:4.04|P-value:1.78E-4 ID:SGTC_2855|Compound:9016525|FD-Score:3.53|P-value:9.14E-4 ID:SGTC_2905|Compound:7799065|FD-Score:-4.76|P-value:1.04E-5 ID:SGTC_2959|Compound:9079155|FD-Score:3.78|P-value:4.21E-4 ID:SGTC_2981|Compound:9013952|FD-Score:4.66|P-value:1.95E-5 ID:SGTC_3012|Compound:9080433|FD-Score:3.64|P-value:6.67E-4 ID:SGTC_3038|Compound:9091290|FD-Score:-3.99|P-value:1.81E-4 ID:SGTC_3132|Compound:9126706|FD-Score:4.78|P-value:1.20E-5 ID:SGTC_3149|Compound:9097855|FD-Score:4.05|P-value:1.75E-4

Top fitness defect scores for YAL023C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_800 3448-7362 5.95 155.0 µM 6.88E-8 translation 51 1.30E-9
2 SGTC_2289 7946404 5.60 165.1 µM 3.65E-7 42 1.07E-8
3 SGTC_2588 indole-3-carbinol 5.48 100.0 µM 6.32E-7 13 2.14E-8
4 SGTC_2589 lobaric acid 5.32 100.0 µM 1.30E-6 9 5.31E-8
5 SGTC_2393 9031399 5.14 185.9 µM 2.72E-6 RNA processing & uracil transport 11 1.35E-7
6 SGTC_452 diindolylmethane 4.97 8.0 µM 5.61E-6 RNA processing & uracil transport 3 3.37E-7
7 SGTC_44 0929-0055 4.97 61.2 µM 5.65E-6 18 3.40E-7
8 SGTC_117 lucigenin 4.93 22.1 µM 6.52E-6 redox potentiating 60 4.07E-7
9 SGTC_1015 4130-4525 4.90 82.0 µM 7.36E-6 60S ribosome export 8 4.74E-7
10 SGTC_3132 9126706 4.78 49.5 µM 1.20E-5 8 8.76E-7
11 SGTC_2981 9013952 4.66 39.0 µM 1.95E-5 25 1.62E-6
12 SGTC_2296 7971656 4.55 126.5 µM 2.92E-5 31 2.69E-6
13 SGTC_2286 7945697 4.28 125.0 µM 7.94E-5 44 9.50E-6
14 SGTC_2581 avocatin a 4.19 64.0 µM 1.08E-4 sphingolipid biosynthesis & PDR1 45 1.40E-5
15 SGTC_351 caspofungin 4.13 25.0 nM 1.33E-4 cell wall 87 1.82E-5
16 SGTC_2447 5741742 4.11 33.8 µM 1.43E-4 15 2.00E-5
17 SGTC_3149 9097855 4.05 49.5 µM 1.75E-4 Golgi 28 2.58E-5
18 SGTC_2802 7925815 4.04 54.5 µM 1.78E-4 36 2.63E-5
19 SGTC_58 0957-0393 3.94 9.0 µM 2.55E-4 cell wall 373 4.13E-5
20 SGTC_2165 5727197 3.83 32.1 µM 3.65E-4 35 6.47E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.153 4.79E-19 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.149 4.38E-18 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.149 4.40E-18 YKL077W_p YKL077W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.148 6.16E-18 YBR021W FUR4 hom Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
0.145 3.03E-17 YBR229C ROT2 hom Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
0.142 1.40E-16 YDL117W CYK3 hom SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants
0.136 2.60E-15 YOR002W ALG6 hom Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
0.124 5.23E-13 YKL048C ELM1 hom Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring
0.124 5.13E-13 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.123 8.61E-13 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.122 1.40E-12 YDR335W MSN5 hom Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm
0.120 2.80E-12 YOR141C ARP8 hom Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity
0.120 3.43E-12 YDL095W PMT1 hom Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen
0.118 7.34E-12 YDR257C RKM4 hom Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein
0.116 1.56E-11 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate