YAL027W / SAW1

5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus

Zygosity: Homozygous strain
fixedexpanded
Profile for YAL027W / SAW1

Click on Significant Values for Screen Details ID:SGTC_17|Compound:0960-0276|FD-Score:-3.07|P-value:4.65E-4 ID:SGTC_48|Compound:0382-0018|FD-Score:3.99|P-value:3.64E-6 ID:SGTC_57|Compound:miconazole|FD-Score:3.18|P-value:1.64E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:3.02|P-value:3.26E-4 ID:SGTC_133|Compound:0724-0033|FD-Score:2.88|P-value:5.64E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:5.18|P-value:3.49E-9 ID:SGTC_273|Compound:hydroxyurea|FD-Score:2.75|P-value:9.21E-4 ID:SGTC_284|Compound:0988-0104|FD-Score:3.98|P-value:3.88E-6 ID:SGTC_306|Compound:1672-3436|FD-Score:-3.19|P-value:2.94E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:-3.27|P-value:2.08E-4 ID:SGTC_321|Compound:4534-3890|FD-Score:3.04|P-value:3.02E-4 ID:SGTC_331|Compound:k048-0133|FD-Score:-3.93|P-value:1.01E-5 ID:SGTC_345|Compound:1362-0200|FD-Score:-3.73|P-value:2.65E-5 ID:SGTC_500|Compound:h-89|FD-Score:4.22|P-value:1.08E-6 ID:SGTC_508|Compound:roscovitine|FD-Score:-3.44|P-value:1.02E-4 ID:SGTC_509|Compound:pifithrin|FD-Score:-2.94|P-value:7.73E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:-3.51|P-value:7.38E-5 ID:SGTC_598|Compound:3937-0236|FD-Score:2.94|P-value:4.45E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:3.99|P-value:3.79E-6 ID:SGTC_740|Compound:k014-0057|FD-Score:2.84|P-value:6.53E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:2.79|P-value:8.01E-4 ID:SGTC_930|Compound:2810-3379|FD-Score:2.87|P-value:5.90E-4 ID:SGTC_1117|Compound:retinoic acid|FD-Score:4.20|P-value:1.24E-6 ID:SGTC_1192|Compound:1488-0095|FD-Score:4.36|P-value:5.05E-7 ID:SGTC_1207|Compound:2099-0101|FD-Score:4.25|P-value:9.37E-7 ID:SGTC_1241|Compound:0364-0022|FD-Score:-2.99|P-value:6.48E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:3.07|P-value:2.69E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:3.60|P-value:2.58E-5 ID:SGTC_1376|Compound:2910-0861|FD-Score:-3.52|P-value:7.06E-5 ID:SGTC_1380|Compound:2291-0730|FD-Score:4.54|P-value:1.81E-7 ID:SGTC_1490|Compound:4089-0339|FD-Score:2.91|P-value:5.03E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:-3.08|P-value:4.43E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:4.49|P-value:2.46E-7 ID:SGTC_1588|Compound:farnesol|FD-Score:2.78|P-value:8.26E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.81|P-value:9.17E-6 ID:SGTC_1611|Compound:st001856|FD-Score:6.03|P-value:8.78E-12 ID:SGTC_1718|Compound:roemerine|FD-Score:3.23|P-value:1.37E-4 ID:SGTC_1750|Compound:st081859|FD-Score:2.77|P-value:8.49E-4 ID:SGTC_1766|Compound:st045414|FD-Score:3.05|P-value:2.82E-4 ID:SGTC_1986|Compound:st071812|FD-Score:-3.32|P-value:1.67E-4 ID:SGTC_2329|Compound:6138490|FD-Score:4.84|P-value:2.94E-8 ID:SGTC_2331|Compound:7980721|FD-Score:4.45|P-value:3.04E-7 ID:SGTC_2358|Compound:9037157|FD-Score:6.29|P-value:1.18E-12 ID:SGTC_2361|Compound:9034121|FD-Score:2.94|P-value:4.43E-4 ID:SGTC_2368|Compound:9071271|FD-Score:-3.71|P-value:3.03E-5 ID:SGTC_2402|Compound:5553605|FD-Score:2.87|P-value:5.86E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:-3.06|P-value:4.89E-4 ID:SGTC_2447|Compound:5741742|FD-Score:3.92|P-value:5.41E-6 ID:SGTC_2449|Compound:5812982|FD-Score:-3.02|P-value:5.59E-4 ID:SGTC_2476|Compound:5806916|FD-Score:2.94|P-value:4.42E-4 ID:SGTC_2483|Compound:5729050|FD-Score:3.30|P-value:1.00E-4 ID:SGTC_2624|Compound:nerol|FD-Score:-2.88|P-value:9.81E-4 ID:SGTC_2691|Compound:st077356|FD-Score:3.68|P-value:1.76E-5 ID:SGTC_3083|Compound:9117465|FD-Score:2.94|P-value:4.50E-4 ID:SGTC_17|Compound:0960-0276|FD-Score:-3.07|P-value:4.65E-4 ID:SGTC_48|Compound:0382-0018|FD-Score:3.99|P-value:3.64E-6 ID:SGTC_57|Compound:miconazole|FD-Score:3.18|P-value:1.64E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:3.02|P-value:3.26E-4 ID:SGTC_133|Compound:0724-0033|FD-Score:2.88|P-value:5.64E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:5.18|P-value:3.49E-9 ID:SGTC_273|Compound:hydroxyurea|FD-Score:2.75|P-value:9.21E-4 ID:SGTC_284|Compound:0988-0104|FD-Score:3.98|P-value:3.88E-6 ID:SGTC_306|Compound:1672-3436|FD-Score:-3.19|P-value:2.94E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:-3.27|P-value:2.08E-4 ID:SGTC_321|Compound:4534-3890|FD-Score:3.04|P-value:3.02E-4 ID:SGTC_331|Compound:k048-0133|FD-Score:-3.93|P-value:1.01E-5 ID:SGTC_345|Compound:1362-0200|FD-Score:-3.73|P-value:2.65E-5 ID:SGTC_500|Compound:h-89|FD-Score:4.22|P-value:1.08E-6 ID:SGTC_508|Compound:roscovitine|FD-Score:-3.44|P-value:1.02E-4 ID:SGTC_509|Compound:pifithrin|FD-Score:-2.94|P-value:7.73E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:-3.51|P-value:7.38E-5 ID:SGTC_598|Compound:3937-0236|FD-Score:2.94|P-value:4.45E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:3.99|P-value:3.79E-6 ID:SGTC_740|Compound:k014-0057|FD-Score:2.84|P-value:6.53E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:2.79|P-value:8.01E-4 ID:SGTC_930|Compound:2810-3379|FD-Score:2.87|P-value:5.90E-4 ID:SGTC_1117|Compound:retinoic acid|FD-Score:4.20|P-value:1.24E-6 ID:SGTC_1192|Compound:1488-0095|FD-Score:4.36|P-value:5.05E-7 ID:SGTC_1207|Compound:2099-0101|FD-Score:4.25|P-value:9.37E-7 ID:SGTC_1241|Compound:0364-0022|FD-Score:-2.99|P-value:6.48E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:3.07|P-value:2.69E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:3.60|P-value:2.58E-5 ID:SGTC_1376|Compound:2910-0861|FD-Score:-3.52|P-value:7.06E-5 ID:SGTC_1380|Compound:2291-0730|FD-Score:4.54|P-value:1.81E-7 ID:SGTC_1490|Compound:4089-0339|FD-Score:2.91|P-value:5.03E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:-3.08|P-value:4.43E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:4.49|P-value:2.46E-7 ID:SGTC_1588|Compound:farnesol|FD-Score:2.78|P-value:8.26E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.81|P-value:9.17E-6 ID:SGTC_1611|Compound:st001856|FD-Score:6.03|P-value:8.78E-12 ID:SGTC_1718|Compound:roemerine|FD-Score:3.23|P-value:1.37E-4 ID:SGTC_1750|Compound:st081859|FD-Score:2.77|P-value:8.49E-4 ID:SGTC_1766|Compound:st045414|FD-Score:3.05|P-value:2.82E-4 ID:SGTC_1986|Compound:st071812|FD-Score:-3.32|P-value:1.67E-4 ID:SGTC_2329|Compound:6138490|FD-Score:4.84|P-value:2.94E-8 ID:SGTC_2331|Compound:7980721|FD-Score:4.45|P-value:3.04E-7 ID:SGTC_2358|Compound:9037157|FD-Score:6.29|P-value:1.18E-12 ID:SGTC_2361|Compound:9034121|FD-Score:2.94|P-value:4.43E-4 ID:SGTC_2368|Compound:9071271|FD-Score:-3.71|P-value:3.03E-5 ID:SGTC_2402|Compound:5553605|FD-Score:2.87|P-value:5.86E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:-3.06|P-value:4.89E-4 ID:SGTC_2447|Compound:5741742|FD-Score:3.92|P-value:5.41E-6 ID:SGTC_2449|Compound:5812982|FD-Score:-3.02|P-value:5.59E-4 ID:SGTC_2476|Compound:5806916|FD-Score:2.94|P-value:4.42E-4 ID:SGTC_2483|Compound:5729050|FD-Score:3.30|P-value:1.00E-4 ID:SGTC_2624|Compound:nerol|FD-Score:-2.88|P-value:9.81E-4 ID:SGTC_2691|Compound:st077356|FD-Score:3.68|P-value:1.76E-5 ID:SGTC_3083|Compound:9117465|FD-Score:2.94|P-value:4.50E-4

Top fitness defect scores for YAL027W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2358 9037157 6.29 4.0 µM 1.18E-12 4 1.64E-10
2 SGTC_1611 st001856 6.03 99.4 µM 8.78E-12 3 8.46E-10
3 SGTC_154 3389-1638 5.18 511.1 µM 3.49E-9 7 1.12E-7
4 SGTC_2329 6138490 4.84 200.0 µM 2.94E-8 10 6.35E-7
5 SGTC_1380 2291-0730 4.54 164.0 µM 1.81E-7 13 2.80E-6
6 SGTC_1584 6-fluoro-dl-tryptophan 4.49 90.0 µM 2.46E-7 mitochondrial processes 7 3.60E-6
7 SGTC_2331 7980721 4.45 200.0 µM 3.04E-7 15 4.27E-6
8 SGTC_1192 1488-0095 4.36 21.4 µM 5.05E-7 10 6.46E-6
9 SGTC_1207 2099-0101 4.25 141.0 µM 9.37E-7 11 1.07E-5
10 SGTC_500 h-89 4.22 112.0 µM 1.08E-6 cell wall signaling 25 1.21E-5
11 SGTC_1117 retinoic acid 4.20 1.1 µM 1.24E-6 11 1.34E-5
12 SGTC_48 0382-0018 3.99 55.0 µM 3.64E-6 7 3.24E-5
13 SGTC_623 k072-0108 3.99 16.9 µM 3.79E-6 heme biosynthesis & mitochondrial translocase 18 3.34E-5
14 SGTC_284 0988-0104 3.98 32.6 µM 3.88E-6 heme requiring 10 3.41E-5
15 SGTC_2447 5741742 3.92 33.8 µM 5.41E-6 20 4.47E-5
16 SGTC_1592 4',5'-dihydroxyflavone 3.81 8.8 µM 9.17E-6 60S ribosome export 14 6.88E-5
17 SGTC_2691 st077356 3.68 60.2 µM 1.76E-5 37 1.17E-4
18 SGTC_1370 2001-0092 3.60 44.6 µM 2.58E-5 49 1.60E-4
19 SGTC_2483 5729050 3.30 27.2 µM 1.00E-4 48 4.83E-4
20 SGTC_1718 roemerine 3.23 11.9 µM 1.37E-4 redox potentiating 97 6.24E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.138 1.09E-15 YBR258C SHG1 hom Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres
0.119 4.17E-12 YJL203W PRP21 het Subunit of the SF3a splicing factor complex, required for spliceosome assembly
0.117 1.00E-11 YAR035W YAT1 hom Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated
0.113 4.38E-11 YMR158W-B_d YMR158W-B_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C
0.099 9.01E-9 YKL171W NNK1 hom Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm
0.094 4.33E-8 YBL074C AAR2 het Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron
0.094 4.89E-8 YLR327C TMA10 hom Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress
0.092 9.50E-8 YHR086W NAM8 hom RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function
0.092 1.07E-7 YKL174C TPO5 hom Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles
0.090 2.00E-7 YBR015C MNN2 hom Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
0.086 6.08E-7 YDL026W_d YDL026W_d hom Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
0.085 8.51E-7 YNL075W IMP4 het Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs
0.084 1.12E-6 YHR042W NCP1 het NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p
0.084 1.21E-6 YDL224C WHI4 hom Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication
0.081 2.46E-6 YIR002C MPH1 hom 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases