YAL032C / PRP45

Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene

Zygosity: Heterozygous strain
fixedexpanded
Profile for YAL032C / PRP45

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:-3.97|P-value:1.61E-5 ID:SGTC_44|Compound:0929-0055|FD-Score:-3.05|P-value:7.03E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:-4.40|P-value:2.13E-6 ID:SGTC_53|Compound:0973-0023|FD-Score:-3.88|P-value:2.41E-5 ID:SGTC_59|Compound:0330-0160|FD-Score:-3.26|P-value:3.23E-4 ID:SGTC_223|Compound:busulfan|FD-Score:3.36|P-value:2.52E-4 ID:SGTC_282|Compound:k297-0036|FD-Score:-3.31|P-value:2.59E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:4.33|P-value:3.62E-6 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.40|P-value:1.85E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:-3.17|P-value:4.40E-4 ID:SGTC_420|Compound:mitomycin C|FD-Score:-3.09|P-value:5.95E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:3.22|P-value:4.44E-4 ID:SGTC_489|Compound:paxilline|FD-Score:-3.54|P-value:1.03E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:-3.29|P-value:2.84E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:3.47|P-value:1.64E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.43|P-value:2.26E-6 ID:SGTC_581|Compound:0958-0271|FD-Score:3.79|P-value:4.45E-5 ID:SGTC_638|Compound:3970-0837|FD-Score:3.54|P-value:1.26E-4 ID:SGTC_686|Compound:1319-0111|FD-Score:3.27|P-value:3.63E-4 ID:SGTC_906|Compound:1165-0455|FD-Score:-3.17|P-value:4.53E-4 ID:SGTC_1030|Compound:k029-0042|FD-Score:-2.97|P-value:9.10E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:3.10|P-value:6.69E-4 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:3.77|P-value:4.73E-5 ID:SGTC_1157|Compound:4092-0394|FD-Score:4.85|P-value:2.50E-7 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.92|P-value:2.47E-5 ID:SGTC_1163|Compound:k060-0048|FD-Score:3.05|P-value:7.99E-4 ID:SGTC_1185|Compound:1319-0113|FD-Score:3.10|P-value:6.74E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.67|P-value:7.37E-5 ID:SGTC_1280|Compound:0880-0253|FD-Score:-3.42|P-value:1.68E-4 ID:SGTC_1289|Compound:0976-0073|FD-Score:3.13|P-value:6.15E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:-3.83|P-value:3.05E-5 ID:SGTC_1328|Compound:1441-0025|FD-Score:3.69|P-value:6.85E-5 ID:SGTC_1454|Compound:4239-0054|FD-Score:3.49|P-value:1.51E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:-9.45|P-value:2.96E-23 ID:SGTC_1486|Compound:3970-0793|FD-Score:3.13|P-value:6.08E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:3.94|P-value:2.26E-5 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:3.32|P-value:2.96E-4 ID:SGTC_1636|Compound:st007707|FD-Score:3.20|P-value:4.74E-4 ID:SGTC_1780|Compound:st038448|FD-Score:3.10|P-value:6.78E-4 ID:SGTC_1871|Compound:st057448|FD-Score:3.18|P-value:5.08E-4 ID:SGTC_1966|Compound:st070145|FD-Score:-3.05|P-value:6.85E-4 ID:SGTC_1978|Compound:st071761|FD-Score:5.77|P-value:1.11E-9 ID:SGTC_1994|Compound:st072947|FD-Score:3.53|P-value:1.33E-4 ID:SGTC_1998|Compound:st075797|FD-Score:3.88|P-value:2.92E-5 ID:SGTC_2000|Compound:st075254|FD-Score:-3.78|P-value:3.85E-5 ID:SGTC_2020|Compound:4017018|FD-Score:4.77|P-value:3.83E-7 ID:SGTC_2093|Compound:5331342|FD-Score:3.05|P-value:8.15E-4 ID:SGTC_2137|Compound:5261020|FD-Score:-3.24|P-value:3.39E-4 ID:SGTC_2147|Compound:5545932|FD-Score:-2.95|P-value:9.71E-4 ID:SGTC_2149|Compound:5554786|FD-Score:3.70|P-value:6.58E-5 ID:SGTC_2205|Compound:7238770|FD-Score:-3.08|P-value:6.19E-4 ID:SGTC_2292|Compound:7671323|FD-Score:4.70|P-value:5.61E-7 ID:SGTC_2431|Compound:5490079|FD-Score:-4.22|P-value:4.98E-6 ID:SGTC_2710|Compound:st078562|FD-Score:-2.97|P-value:9.27E-4 ID:SGTC_2796|Compound:7949634|FD-Score:-2.99|P-value:8.42E-4 ID:SGTC_2905|Compound:7799065|FD-Score:-9.92|P-value:2.19E-25 ID:SGTC_2908|Compound:7930301|FD-Score:-4.06|P-value:1.06E-5 ID:SGTC_3116|Compound:9124943|FD-Score:3.02|P-value:9.02E-4 ID:SGTC_3129|Compound:9127257|FD-Score:3.85|P-value:3.39E-5 ID:SGTC_3257|Compound:9139206|FD-Score:3.69|P-value:6.70E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:-3.97|P-value:1.61E-5 ID:SGTC_44|Compound:0929-0055|FD-Score:-3.05|P-value:7.03E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:-4.40|P-value:2.13E-6 ID:SGTC_53|Compound:0973-0023|FD-Score:-3.88|P-value:2.41E-5 ID:SGTC_59|Compound:0330-0160|FD-Score:-3.26|P-value:3.23E-4 ID:SGTC_223|Compound:busulfan|FD-Score:3.36|P-value:2.52E-4 ID:SGTC_282|Compound:k297-0036|FD-Score:-3.31|P-value:2.59E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:4.33|P-value:3.62E-6 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.40|P-value:1.85E-4 ID:SGTC_374|Compound:0880-0231|FD-Score:-3.17|P-value:4.40E-4 ID:SGTC_420|Compound:mitomycin C|FD-Score:-3.09|P-value:5.95E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:3.22|P-value:4.44E-4 ID:SGTC_489|Compound:paxilline|FD-Score:-3.54|P-value:1.03E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:-3.29|P-value:2.84E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:3.47|P-value:1.64E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.43|P-value:2.26E-6 ID:SGTC_581|Compound:0958-0271|FD-Score:3.79|P-value:4.45E-5 ID:SGTC_638|Compound:3970-0837|FD-Score:3.54|P-value:1.26E-4 ID:SGTC_686|Compound:1319-0111|FD-Score:3.27|P-value:3.63E-4 ID:SGTC_906|Compound:1165-0455|FD-Score:-3.17|P-value:4.53E-4 ID:SGTC_1030|Compound:k029-0042|FD-Score:-2.97|P-value:9.10E-4 ID:SGTC_1095|Compound:3-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran|FD-Score:3.10|P-value:6.69E-4 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:3.77|P-value:4.73E-5 ID:SGTC_1157|Compound:4092-0394|FD-Score:4.85|P-value:2.50E-7 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.92|P-value:2.47E-5 ID:SGTC_1163|Compound:k060-0048|FD-Score:3.05|P-value:7.99E-4 ID:SGTC_1185|Compound:1319-0113|FD-Score:3.10|P-value:6.74E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.67|P-value:7.37E-5 ID:SGTC_1280|Compound:0880-0253|FD-Score:-3.42|P-value:1.68E-4 ID:SGTC_1289|Compound:0976-0073|FD-Score:3.13|P-value:6.15E-4 ID:SGTC_1291|Compound:0993-0025|FD-Score:-3.83|P-value:3.05E-5 ID:SGTC_1328|Compound:1441-0025|FD-Score:3.69|P-value:6.85E-5 ID:SGTC_1454|Compound:4239-0054|FD-Score:3.49|P-value:1.51E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:-9.45|P-value:2.96E-23 ID:SGTC_1486|Compound:3970-0793|FD-Score:3.13|P-value:6.08E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:3.94|P-value:2.26E-5 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:3.32|P-value:2.96E-4 ID:SGTC_1636|Compound:st007707|FD-Score:3.20|P-value:4.74E-4 ID:SGTC_1780|Compound:st038448|FD-Score:3.10|P-value:6.78E-4 ID:SGTC_1871|Compound:st057448|FD-Score:3.18|P-value:5.08E-4 ID:SGTC_1966|Compound:st070145|FD-Score:-3.05|P-value:6.85E-4 ID:SGTC_1978|Compound:st071761|FD-Score:5.77|P-value:1.11E-9 ID:SGTC_1994|Compound:st072947|FD-Score:3.53|P-value:1.33E-4 ID:SGTC_1998|Compound:st075797|FD-Score:3.88|P-value:2.92E-5 ID:SGTC_2000|Compound:st075254|FD-Score:-3.78|P-value:3.85E-5 ID:SGTC_2020|Compound:4017018|FD-Score:4.77|P-value:3.83E-7 ID:SGTC_2093|Compound:5331342|FD-Score:3.05|P-value:8.15E-4 ID:SGTC_2137|Compound:5261020|FD-Score:-3.24|P-value:3.39E-4 ID:SGTC_2147|Compound:5545932|FD-Score:-2.95|P-value:9.71E-4 ID:SGTC_2149|Compound:5554786|FD-Score:3.70|P-value:6.58E-5 ID:SGTC_2205|Compound:7238770|FD-Score:-3.08|P-value:6.19E-4 ID:SGTC_2292|Compound:7671323|FD-Score:4.70|P-value:5.61E-7 ID:SGTC_2431|Compound:5490079|FD-Score:-4.22|P-value:4.98E-6 ID:SGTC_2710|Compound:st078562|FD-Score:-2.97|P-value:9.27E-4 ID:SGTC_2796|Compound:7949634|FD-Score:-2.99|P-value:8.42E-4 ID:SGTC_2905|Compound:7799065|FD-Score:-9.92|P-value:2.19E-25 ID:SGTC_2908|Compound:7930301|FD-Score:-4.06|P-value:1.06E-5 ID:SGTC_3116|Compound:9124943|FD-Score:3.02|P-value:9.02E-4 ID:SGTC_3129|Compound:9127257|FD-Score:3.85|P-value:3.39E-5 ID:SGTC_3257|Compound:9139206|FD-Score:3.69|P-value:6.70E-5

Top fitness defect scores for YAL032C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1978 st071761 5.77 42.0 µM 1.11E-9 TSC3-RPN4 2 4.01E-9 0.22
2 SGTC_1157 4092-0394 4.85 27.6 µM 2.50E-7 2 6.13E-7 0.68
3 SGTC_2020 4017018 4.77 200.0 µM 3.83E-7 5 9.10E-7 0.63
4 SGTC_2292 7671323 4.70 168.1 µM 5.61E-7 RNA processing & uracil transport 8 1.30E-6 0.12
5 SGTC_568 1193-0097 4.43 7.5 µM 2.26E-6 ERAD & cell cycle 22 4.76E-6 0.13
6 SGTC_361 000s-0510 4.33 1.4 µM 3.62E-6 5 7.36E-6 0.16
7 SGTC_1578 josamycin 3.94 24.2 µM 2.26E-5 6 4.05E-5 0.43
8 SGTC_1159 4092-0538 3.92 61.2 µM 2.47E-5 3 4.40E-5 0.06
9 SGTC_1998 st075797 3.88 76.8 µM 2.92E-5 mitochondrial response to ROS 2 5.15E-5 0.35
10 SGTC_3129 9127257 3.85 49.5 µM 3.39E-5 7 5.91E-5 0.25
11 SGTC_581 0958-0271 3.79 211.0 µM 4.45E-5 7 7.62E-5 0.25
12 SGTC_1099 5-nonyloxytryptamine 3.77 2.9 µM 4.73E-5 5 8.06E-5 0.35
13 SGTC_2149 5554786 3.70 194.8 µM 6.58E-5 cell wall signaling 9 1.10E-4 0.11
14 SGTC_3257 9139206 3.69 33.0 µM 6.70E-5 6 1.12E-4 0.06
15 SGTC_1328 1441-0025 3.69 69.2 µM 6.85E-5 4 1.14E-4 0.12
16 SGTC_1203 3992-3522 3.67 103.0 µM 7.37E-5 16 1.22E-4 0.06
17 SGTC_638 3970-0837 3.54 4.1 µM 1.26E-4 8 2.01E-4 0.04
18 SGTC_1994 st072947 3.53 40.9 µM 1.33E-4 11 2.11E-4 0.04
19 SGTC_1454 4239-0054 3.49 42.6 µM 1.51E-4 8 2.37E-4 0.40
20 SGTC_540 1187-0821 3.47 17.3 µM 1.64E-4 11 2.57E-4 0.24

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.171 2.35E-23 YAL034C-B_d YAL034C-B_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.171 2.69E-23 YAL003W EFB1 het Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site
0.148 6.17E-18 YGL207W SPT16 het Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome
0.144 4.65E-17 YNR046W TRM112 het Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress
0.133 1.12E-14 YBR155W CNS1 het TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion
0.131 2.68E-14 YBR029C CDS1 het Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids
0.109 2.23E-10 YBR078W ECM33 hom GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p
0.108 4.08E-10 YAR019C CDC15 het Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress
0.095 3.18E-8 YBR038W CHS2 het Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis
0.090 1.86E-7 YJL025W RRN7 het Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
0.087 4.16E-7 YJL001W PRE3 het Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
0.084 9.89E-7 YER014C-A BUD25 hom Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern
0.083 1.69E-6 YIL003W CFD1 het Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol
0.082 2.16E-6 YJR017C ESS1 het Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3
0.081 2.68E-6 YBR049C REB1 het RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication