YAL043C / PTA1

Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII

Zygosity: Heterozygous strain
fixedexpanded
Profile for YAL043C / PTA1

Click on Significant Values for Screen Details ID:SGTC_18|Compound:0111-0177|FD-Score:3.07|P-value:1.72E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:3.74|P-value:6.40E-6 ID:SGTC_163|Compound:1327-0036|FD-Score:3.82|P-value:4.16E-6 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.34|P-value:4.92E-5 ID:SGTC_359|Compound:0081-0058|FD-Score:-3.36|P-value:3.59E-5 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:2.75|P-value:6.78E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:2.68|P-value:8.85E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:-3.93|P-value:1.73E-6 ID:SGTC_767|Compound:0929-0023|FD-Score:4.51|P-value:6.78E-8 ID:SGTC_773|Compound:4358-1457|FD-Score:8.05|P-value:1.95E-21 ID:SGTC_826|Compound:1683-5028|FD-Score:2.88|P-value:3.89E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:-2.93|P-value:2.62E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:-2.76|P-value:5.50E-4 ID:SGTC_1180|Compound:1193-0155|FD-Score:-3.02|P-value:1.73E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:-3.72|P-value:5.62E-6 ID:SGTC_1319|Compound:1306-0155|FD-Score:2.89|P-value:3.84E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:-2.94|P-value:2.56E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:-3.97|P-value:1.38E-6 ID:SGTC_1376|Compound:2910-0861|FD-Score:-3.49|P-value:1.89E-5 ID:SGTC_1469|Compound:k284-2957|FD-Score:-3.50|P-value:1.78E-5 ID:SGTC_1846|Compound:st056244|FD-Score:2.73|P-value:7.28E-4 ID:SGTC_1892|Compound:st058411|FD-Score:2.77|P-value:6.19E-4 ID:SGTC_2017|Compound:5132927|FD-Score:-2.84|P-value:3.93E-4 ID:SGTC_2555|Compound:cholic acid|FD-Score:2.70|P-value:8.35E-4 ID:SGTC_2708|Compound:st078559|FD-Score:2.95|P-value:2.88E-4 ID:SGTC_3336|Compound:9143619|FD-Score:3.16|P-value:1.12E-4 ID:SGTC_3344|Compound:9148862|FD-Score:2.85|P-value:4.57E-4 ID:SGTC_18|Compound:0111-0177|FD-Score:3.07|P-value:1.72E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:3.74|P-value:6.40E-6 ID:SGTC_163|Compound:1327-0036|FD-Score:3.82|P-value:4.16E-6 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.34|P-value:4.92E-5 ID:SGTC_359|Compound:0081-0058|FD-Score:-3.36|P-value:3.59E-5 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:2.75|P-value:6.78E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:2.68|P-value:8.85E-4 ID:SGTC_760|Compound:0568-0725|FD-Score:-3.93|P-value:1.73E-6 ID:SGTC_767|Compound:0929-0023|FD-Score:4.51|P-value:6.78E-8 ID:SGTC_773|Compound:4358-1457|FD-Score:8.05|P-value:1.95E-21 ID:SGTC_826|Compound:1683-5028|FD-Score:2.88|P-value:3.89E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:-2.93|P-value:2.62E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:-2.76|P-value:5.50E-4 ID:SGTC_1180|Compound:1193-0155|FD-Score:-3.02|P-value:1.73E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:-3.72|P-value:5.62E-6 ID:SGTC_1319|Compound:1306-0155|FD-Score:2.89|P-value:3.84E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:-2.94|P-value:2.56E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:-3.97|P-value:1.38E-6 ID:SGTC_1376|Compound:2910-0861|FD-Score:-3.49|P-value:1.89E-5 ID:SGTC_1469|Compound:k284-2957|FD-Score:-3.50|P-value:1.78E-5 ID:SGTC_1846|Compound:st056244|FD-Score:2.73|P-value:7.28E-4 ID:SGTC_1892|Compound:st058411|FD-Score:2.77|P-value:6.19E-4 ID:SGTC_2017|Compound:5132927|FD-Score:-2.84|P-value:3.93E-4 ID:SGTC_2555|Compound:cholic acid|FD-Score:2.70|P-value:8.35E-4 ID:SGTC_2708|Compound:st078559|FD-Score:2.95|P-value:2.88E-4 ID:SGTC_3336|Compound:9143619|FD-Score:3.16|P-value:1.12E-4 ID:SGTC_3344|Compound:9148862|FD-Score:2.85|P-value:4.57E-4

Top fitness defect scores for YAL043C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_773 4358-1457 8.05 44.6 µM 1.95E-21 4 3.99E-16 0.88
2 SGTC_767 0929-0023 4.51 86.0 µM 6.78E-8 TRP & mitochondrial translation 9 3.24E-6 0.21
3 SGTC_163 1327-0036 3.82 7.5 µM 4.16E-6 48 6.76E-5 0.09
4 SGTC_122 0236-0016 3.74 402.4 µM 6.40E-6 RNA processing & uracil transport 17 9.31E-5 0.02
5 SGTC_234 nsc-17383 3.34 10.7 µM 4.92E-5 ERG2 9 4.22E-4 0.02
6 SGTC_3336 9143619 3.16 71.4 µM 1.12E-4 2 7.80E-4 0.22
7 SGTC_18 0111-0177 3.07 500.0 nM 1.72E-4 12 0.00107 0.00
8 SGTC_2708 st078559 2.95 56.0 µM 2.88E-4 6 0.00157 0.01
9 SGTC_1319 1306-0155 2.89 43.7 µM 3.84E-4 17 0.00195 0.05
10 SGTC_826 1683-5028 2.88 118.0 µM 3.89E-4 11 0.00197 0.06
11 SGTC_3344 9148862 2.85 3.9 µM 4.57E-4 13 0.00222 0.01
12 SGTC_1892 st058411 2.77 7.4 µM 6.19E-4 18 0.00279 0.03
13 SGTC_388 3',4'-dichloroisocoumarin 2.75 16.7 µM 6.78E-4 21 0.00298 0.01
14 SGTC_1846 st056244 2.73 14.8 µM 7.28E-4 19 0.00314 0.11
15 SGTC_2555 cholic acid 2.70 100.0 µM 8.35E-4 8 0.00348 0.06
16 SGTC_457 grayanotoxin iii 2.68 135.0 µM 8.85E-4 38 0.00364 0.02
17 SGTC_1026 k007-1105 2.64 30.6 µM 0.00104 ERAD & cell cycle 42 0.00411 0.03
18 SGTC_902 1120-0002 2.64 2.1 µM 0.00104 azole & statin 34 0.00411 0.02
19 SGTC_1861 st057647 2.64 17.4 µM 0.00106 17 0.00417 0.02
20 SGTC_632 0781-2353 2.61 4.7 µM 0.00119 Golgi 42 0.00454 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.213 9.55E-36 YLR053C_p YLR053C_p hom Putative protein of unknown function
0.196 4.20E-30 YCL026C-A FRM2 hom Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin
0.194 7.56E-30 YLR212C TUB4 het Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress
0.194 1.30E-29 YDR055W PST1 hom Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1
0.188 5.27E-28 YFL024C EPL1 het Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
0.170 4.52E-23 YIR035C_p YIR035C_p hom Putative cytoplasmic short-chain dehydrogenase/reductase
0.158 3.16E-20 YKR054C DYN1 hom Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p
0.146 1.52E-17 YER052C HOM3 hom Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.145 4.12E-17 YGL220W FRA2 hom Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel
0.145 3.18E-17 YOR238W_p YOR238W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.144 4.68E-17 YGR193C PDX1 hom Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core
0.142 1.33E-16 YDL104C QRI7 hom Highly conserved mitochondrial protein, essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification
0.139 5.16E-16 YIL003W CFD1 het Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol
0.139 6.27E-16 YJR001W AVT1 hom Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.138 1.05E-15 YDR166C SEC5 het Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip