YAL044C / GCV3

H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF

Zygosity: Homozygous strain
fixedexpanded
Profile for YAL044C / GCV3

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:5.19|P-value:2.59E-6 ID:SGTC_236|Compound:1509-0011|FD-Score:3.63|P-value:7.39E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:-4.12|P-value:1.26E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:4.86|P-value:1.01E-5 ID:SGTC_440|Compound:fluspirilene|FD-Score:4.32|P-value:7.46E-5 ID:SGTC_495|Compound:decylubiquinone|FD-Score:4.52|P-value:3.66E-5 ID:SGTC_496|Compound:calpeptin|FD-Score:4.57|P-value:3.01E-5 ID:SGTC_535|Compound:2293-4129|FD-Score:-4.57|P-value:2.53E-5 ID:SGTC_570|Compound:0987-0065|FD-Score:-4.30|P-value:6.63E-5 ID:SGTC_624|Compound:k048-0052|FD-Score:4.35|P-value:6.87E-5 ID:SGTC_697|Compound:0508-1955|FD-Score:4.72|P-value:1.75E-5 ID:SGTC_751|Compound:0833-0568|FD-Score:3.92|P-value:2.90E-4 ID:SGTC_762|Compound:1165-0429|FD-Score:-3.55|P-value:8.02E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:-4.21|P-value:9.14E-5 ID:SGTC_811|Compound:0416-0114|FD-Score:5.90|P-value:1.07E-7 ID:SGTC_852|Compound:0418-0143|FD-Score:3.71|P-value:5.81E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:4.15|P-value:1.36E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:3.61|P-value:7.89E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:4.59|P-value:2.85E-5 ID:SGTC_1165|Compound:k072-0275|FD-Score:4.02|P-value:2.13E-4 ID:SGTC_1170|Compound:0310-0127|FD-Score:6.27|P-value:1.78E-8 ID:SGTC_1317|Compound:1273-0028|FD-Score:4.02|P-value:2.15E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:4.53|P-value:3.49E-5 ID:SGTC_1372|Compound:2752-0194|FD-Score:4.07|P-value:1.81E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:4.06|P-value:1.87E-4 ID:SGTC_1432|Compound:4049-0298|FD-Score:-4.06|P-value:1.57E-4 ID:SGTC_1480|Compound:1070-0044|FD-Score:6.11|P-value:3.89E-8 ID:SGTC_1496|Compound:4469-0713|FD-Score:3.95|P-value:2.68E-4 ID:SGTC_1534|Compound:6-azauridine|FD-Score:4.40|P-value:5.59E-5 ID:SGTC_1554|Compound:st069299|FD-Score:4.50|P-value:3.88E-5 ID:SGTC_1639|Compound:Lupinine o-methylbenzoicacid ester hydrochloride|FD-Score:3.89|P-value:3.22E-4 ID:SGTC_1651|Compound:st011928|FD-Score:5.41|P-value:1.00E-6 ID:SGTC_1667|Compound:st014849|FD-Score:3.57|P-value:8.76E-4 ID:SGTC_1788|Compound:fluconazole|FD-Score:3.56|P-value:9.05E-4 ID:SGTC_1833|Compound:st055984|FD-Score:3.58|P-value:8.44E-4 ID:SGTC_2043|Compound:5101342|FD-Score:3.58|P-value:8.59E-4 ID:SGTC_2254|Compound:7938975|FD-Score:-3.62|P-value:6.51E-4 ID:SGTC_2299|Compound:7976368|FD-Score:3.87|P-value:3.49E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:8.47|P-value:4.65E-14 ID:SGTC_2418|Compound:pentamidine|FD-Score:6.99|P-value:4.06E-10 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:-3.65|P-value:5.91E-4 ID:SGTC_2672|Compound:12a-hydroxy-5-deoxydehydromunduserone|FD-Score:3.91|P-value:3.00E-4 ID:SGTC_2676|Compound:harmalol|FD-Score:-3.70|P-value:5.05E-4 ID:SGTC_2718|Compound:lomofungin|FD-Score:3.65|P-value:7.00E-4 ID:SGTC_2729|Compound:fluphenazine|FD-Score:3.95|P-value:2.70E-4 ID:SGTC_2748|Compound:diazoxide|FD-Score:3.92|P-value:2.95E-4 ID:SGTC_2894|Compound:9048625|FD-Score:3.61|P-value:7.74E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-3.88|P-value:2.88E-4 ID:SGTC_2965|Compound:9082772|FD-Score:4.64|P-value:2.36E-5 ID:SGTC_3052|Compound:9092717|FD-Score:3.84|P-value:3.78E-4 ID:SGTC_3085|Compound:9117376|FD-Score:4.08|P-value:1.76E-4 ID:SGTC_3093|Compound:9117344|FD-Score:3.83|P-value:3.90E-4 ID:SGTC_3111|Compound:9122088|FD-Score:-3.97|P-value:2.08E-4 ID:SGTC_3116|Compound:9124943|FD-Score:5.74|P-value:2.31E-7 ID:SGTC_3133|Compound:9127736|FD-Score:4.95|P-value:6.96E-6 ID:SGTC_3142|Compound:9086498|FD-Score:-4.15|P-value:1.16E-4 ID:SGTC_3165|Compound:9101544|FD-Score:-3.55|P-value:8.07E-4 ID:SGTC_3246|Compound:9135460|FD-Score:3.53|P-value:9.85E-4 ID:SGTC_3252|Compound:9135871|FD-Score:3.93|P-value:2.84E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:5.19|P-value:2.59E-6 ID:SGTC_236|Compound:1509-0011|FD-Score:3.63|P-value:7.39E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:-4.12|P-value:1.26E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:4.86|P-value:1.01E-5 ID:SGTC_440|Compound:fluspirilene|FD-Score:4.32|P-value:7.46E-5 ID:SGTC_495|Compound:decylubiquinone|FD-Score:4.52|P-value:3.66E-5 ID:SGTC_496|Compound:calpeptin|FD-Score:4.57|P-value:3.01E-5 ID:SGTC_535|Compound:2293-4129|FD-Score:-4.57|P-value:2.53E-5 ID:SGTC_570|Compound:0987-0065|FD-Score:-4.30|P-value:6.63E-5 ID:SGTC_624|Compound:k048-0052|FD-Score:4.35|P-value:6.87E-5 ID:SGTC_697|Compound:0508-1955|FD-Score:4.72|P-value:1.75E-5 ID:SGTC_751|Compound:0833-0568|FD-Score:3.92|P-value:2.90E-4 ID:SGTC_762|Compound:1165-0429|FD-Score:-3.55|P-value:8.02E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:-4.21|P-value:9.14E-5 ID:SGTC_811|Compound:0416-0114|FD-Score:5.90|P-value:1.07E-7 ID:SGTC_852|Compound:0418-0143|FD-Score:3.71|P-value:5.81E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:4.15|P-value:1.36E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:3.61|P-value:7.89E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:4.59|P-value:2.85E-5 ID:SGTC_1165|Compound:k072-0275|FD-Score:4.02|P-value:2.13E-4 ID:SGTC_1170|Compound:0310-0127|FD-Score:6.27|P-value:1.78E-8 ID:SGTC_1317|Compound:1273-0028|FD-Score:4.02|P-value:2.15E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:4.53|P-value:3.49E-5 ID:SGTC_1372|Compound:2752-0194|FD-Score:4.07|P-value:1.81E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:4.06|P-value:1.87E-4 ID:SGTC_1432|Compound:4049-0298|FD-Score:-4.06|P-value:1.57E-4 ID:SGTC_1480|Compound:1070-0044|FD-Score:6.11|P-value:3.89E-8 ID:SGTC_1496|Compound:4469-0713|FD-Score:3.95|P-value:2.68E-4 ID:SGTC_1534|Compound:6-azauridine|FD-Score:4.40|P-value:5.59E-5 ID:SGTC_1554|Compound:st069299|FD-Score:4.50|P-value:3.88E-5 ID:SGTC_1639|Compound:Lupinine o-methylbenzoicacid ester hydrochloride|FD-Score:3.89|P-value:3.22E-4 ID:SGTC_1651|Compound:st011928|FD-Score:5.41|P-value:1.00E-6 ID:SGTC_1667|Compound:st014849|FD-Score:3.57|P-value:8.76E-4 ID:SGTC_1788|Compound:fluconazole|FD-Score:3.56|P-value:9.05E-4 ID:SGTC_1833|Compound:st055984|FD-Score:3.58|P-value:8.44E-4 ID:SGTC_2043|Compound:5101342|FD-Score:3.58|P-value:8.59E-4 ID:SGTC_2254|Compound:7938975|FD-Score:-3.62|P-value:6.51E-4 ID:SGTC_2299|Compound:7976368|FD-Score:3.87|P-value:3.49E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:8.47|P-value:4.65E-14 ID:SGTC_2418|Compound:pentamidine|FD-Score:6.99|P-value:4.06E-10 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:-3.65|P-value:5.91E-4 ID:SGTC_2672|Compound:12a-hydroxy-5-deoxydehydromunduserone|FD-Score:3.91|P-value:3.00E-4 ID:SGTC_2676|Compound:harmalol|FD-Score:-3.70|P-value:5.05E-4 ID:SGTC_2718|Compound:lomofungin|FD-Score:3.65|P-value:7.00E-4 ID:SGTC_2729|Compound:fluphenazine|FD-Score:3.95|P-value:2.70E-4 ID:SGTC_2748|Compound:diazoxide|FD-Score:3.92|P-value:2.95E-4 ID:SGTC_2894|Compound:9048625|FD-Score:3.61|P-value:7.74E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-3.88|P-value:2.88E-4 ID:SGTC_2965|Compound:9082772|FD-Score:4.64|P-value:2.36E-5 ID:SGTC_3052|Compound:9092717|FD-Score:3.84|P-value:3.78E-4 ID:SGTC_3085|Compound:9117376|FD-Score:4.08|P-value:1.76E-4 ID:SGTC_3093|Compound:9117344|FD-Score:3.83|P-value:3.90E-4 ID:SGTC_3111|Compound:9122088|FD-Score:-3.97|P-value:2.08E-4 ID:SGTC_3116|Compound:9124943|FD-Score:5.74|P-value:2.31E-7 ID:SGTC_3133|Compound:9127736|FD-Score:4.95|P-value:6.96E-6 ID:SGTC_3142|Compound:9086498|FD-Score:-4.15|P-value:1.16E-4 ID:SGTC_3165|Compound:9101544|FD-Score:-3.55|P-value:8.07E-4 ID:SGTC_3246|Compound:9135460|FD-Score:3.53|P-value:9.85E-4 ID:SGTC_3252|Compound:9135871|FD-Score:3.93|P-value:2.84E-4

Top fitness defect scores for YAL044C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2406 ethylparaben 8.47 405.7 µM 4.65E-14 1 1.24E-17
2 SGTC_2418 pentamidine 6.99 31.7 µM 4.06E-10 mitochondrial processes 9 1.41E-12
3 SGTC_1170 0310-0127 6.27 64.8 µM 1.78E-8 3 1.79E-10
4 SGTC_1480 1070-0044 6.11 41.2 µM 3.89E-8 1 4.89E-10
5 SGTC_811 0416-0114 5.90 265.0 µM 1.07E-7 3 1.78E-9
6 SGTC_3116 9124943 5.74 49.5 µM 2.31E-7 3 4.76E-9
7 SGTC_1651 st011928 5.41 86.1 µM 1.00E-6 RSC complex & mRNA processing 5 3.13E-8
8 SGTC_36 0335-0881 5.19 14.2 µM 2.59E-6 ubiquinone biosynthesis & proteasome 81 1.05E-7
9 SGTC_3133 9127736 4.95 49.5 µM 6.96E-6 9 3.72E-7
10 SGTC_421 streptovitacin 4.86 30.0 mM 1.01E-5 9 5.95E-7
11 SGTC_697 0508-1955 4.72 564.0 µM 1.75E-5 18 1.21E-6
12 SGTC_2965 9082772 4.64 38.6 µM 2.36E-5 mitochondrial response to ROS 25 1.77E-6
13 SGTC_1036 k216-0215 4.59 33.5 µM 2.85E-5 mitochondrial response to ROS 33 2.25E-6
14 SGTC_496 calpeptin 4.57 138.0 µM 3.01E-5 8 2.42E-6
15 SGTC_1350 1495-0454 4.53 146.0 µM 3.49E-5 12 2.92E-6
16 SGTC_495 decylubiquinone 4.52 155.0 µM 3.66E-5 copper-dependent oxidative stress 12 3.10E-6
17 SGTC_1554 st069299 4.50 38.7 µM 3.88E-5 10 3.34E-6
18 SGTC_1534 6-azauridine 4.40 81.6 µM 5.59E-5 RNA pol III & RNase P/MRP 12 5.32E-6
19 SGTC_624 k048-0052 4.35 28.0 µM 6.87E-5 Golgi 10 6.93E-6
20 SGTC_440 fluspirilene 4.32 35.1 µM 7.46E-5 8 7.69E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.174 3.78E-24 YHR067W HTD2 hom Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology
0.130 3.51E-14 YER154W OXA1 hom Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals
0.126 2.55E-13 YBR179C FZO1 hom Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system
0.123 9.47E-13 YJL046W AIM22 hom Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss
0.111 1.28E-10 YGR257C MTM1 hom Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor
0.109 2.13E-10 YMR257C PET111 hom Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane
0.108 3.09E-10 YKL055C OAR1 hom Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p
0.105 1.23E-9 YPL148C PPT2 hom Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation
0.104 1.58E-9 YDR268W MSW1 hom Mitochondrial tryptophanyl-tRNA synthetase
0.103 2.48E-9 YDR462W MRPL28 hom Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
0.102 3.07E-9 YDR298C ATP5 hom Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated
0.102 3.11E-9 YKR018C YKR018C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication
0.102 3.32E-9 YLR091W GEP5 hom Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine
0.100 7.47E-9 YKL016C ATP7 hom Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
0.099 9.05E-9 YGR102C GTF1 hom Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria