YAR002W / NUP60

FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153

Zygosity: Homozygous strain
fixedexpanded
Profile for YAR002W / NUP60

Click on Significant Values for Screen Details ID:SGTC_53|Compound:0973-0023|FD-Score:-3.77|P-value:3.09E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:4.14|P-value:7.75E-5 ID:SGTC_458|Compound:nppb|FD-Score:4.42|P-value:2.76E-5 ID:SGTC_512|Compound:prima-1|FD-Score:4.81|P-value:5.59E-6 ID:SGTC_528|Compound:3492-0019|FD-Score:5.29|P-value:6.89E-7 ID:SGTC_542|Compound:3381-0244|FD-Score:3.40|P-value:9.63E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:3.63|P-value:4.55E-4 ID:SGTC_713|Compound:1488-1073|FD-Score:-3.53|P-value:6.95E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:4.34|P-value:3.77E-5 ID:SGTC_745|Compound:0469-0705|FD-Score:4.56|P-value:1.55E-5 ID:SGTC_965|Compound:1291-5240|FD-Score:7.24|P-value:2.02E-11 ID:SGTC_1003|Compound:4049-0218|FD-Score:4.09|P-value:9.60E-5 ID:SGTC_1031|Compound:k035-0009|FD-Score:3.93|P-value:1.69E-4 ID:SGTC_1041|Compound:0206-0046|FD-Score:3.87|P-value:2.08E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:-4.14|P-value:8.67E-5 ID:SGTC_1134|Compound:3477-0009|FD-Score:-3.73|P-value:3.56E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:4.83|P-value:5.24E-6 ID:SGTC_1260|Compound:0700-1617|FD-Score:3.46|P-value:7.92E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.80|P-value:2.62E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:3.47|P-value:7.59E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:6.18|P-value:8.67E-9 ID:SGTC_1512|Compound:0259-0544|FD-Score:3.96|P-value:1.50E-4 ID:SGTC_1516|Compound:st013856|FD-Score:4.97|P-value:2.83E-6 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:12.80|P-value:1.27E-31 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.20|P-value:6.19E-5 ID:SGTC_1602|Compound:st001878|FD-Score:-3.55|P-value:6.52E-4 ID:SGTC_1607|Compound:st000305|FD-Score:4.04|P-value:1.15E-4 ID:SGTC_1804|Compound:st050076|FD-Score:3.99|P-value:1.36E-4 ID:SGTC_1807|Compound:st050092|FD-Score:5.40|P-value:4.26E-7 ID:SGTC_1881|Compound:5652484|FD-Score:4.32|P-value:4.02E-5 ID:SGTC_2010|Compound:4012422|FD-Score:4.44|P-value:2.56E-5 ID:SGTC_2029|Compound:5175081|FD-Score:5.37|P-value:4.72E-7 ID:SGTC_2057|Compound:5236571|FD-Score:4.02|P-value:1.19E-4 ID:SGTC_2220|Compound:6782180|FD-Score:4.32|P-value:4.10E-5 ID:SGTC_2437|Compound:5741531|FD-Score:-3.69|P-value:4.04E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:5.26|P-value:8.12E-7 ID:SGTC_2619|Compound:obliquin|FD-Score:4.44|P-value:2.58E-5 ID:SGTC_2710|Compound:st078562|FD-Score:4.29|P-value:4.58E-5 ID:SGTC_2729|Compound:fluphenazine|FD-Score:4.74|P-value:7.62E-6 ID:SGTC_2814|Compound:7977482|FD-Score:4.41|P-value:2.81E-5 ID:SGTC_2957|Compound:9076800|FD-Score:5.87|P-value:4.27E-8 ID:SGTC_3012|Compound:9080433|FD-Score:4.20|P-value:6.31E-5 ID:SGTC_3259|Compound:9138485|FD-Score:-3.42|P-value:9.57E-4 ID:SGTC_3334|Compound:9142812|FD-Score:3.80|P-value:2.60E-4 ID:SGTC_3337|Compound:9144342|FD-Score:3.45|P-value:8.22E-4 ID:SGTC_3338|Compound:9144300|FD-Score:4.41|P-value:2.86E-5 ID:SGTC_53|Compound:0973-0023|FD-Score:-3.77|P-value:3.09E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:4.14|P-value:7.75E-5 ID:SGTC_458|Compound:nppb|FD-Score:4.42|P-value:2.76E-5 ID:SGTC_512|Compound:prima-1|FD-Score:4.81|P-value:5.59E-6 ID:SGTC_528|Compound:3492-0019|FD-Score:5.29|P-value:6.89E-7 ID:SGTC_542|Compound:3381-0244|FD-Score:3.40|P-value:9.63E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:3.63|P-value:4.55E-4 ID:SGTC_713|Compound:1488-1073|FD-Score:-3.53|P-value:6.95E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:4.34|P-value:3.77E-5 ID:SGTC_745|Compound:0469-0705|FD-Score:4.56|P-value:1.55E-5 ID:SGTC_965|Compound:1291-5240|FD-Score:7.24|P-value:2.02E-11 ID:SGTC_1003|Compound:4049-0218|FD-Score:4.09|P-value:9.60E-5 ID:SGTC_1031|Compound:k035-0009|FD-Score:3.93|P-value:1.69E-4 ID:SGTC_1041|Compound:0206-0046|FD-Score:3.87|P-value:2.08E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:-4.14|P-value:8.67E-5 ID:SGTC_1134|Compound:3477-0009|FD-Score:-3.73|P-value:3.56E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:4.83|P-value:5.24E-6 ID:SGTC_1260|Compound:0700-1617|FD-Score:3.46|P-value:7.92E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.80|P-value:2.62E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:3.47|P-value:7.59E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:6.18|P-value:8.67E-9 ID:SGTC_1512|Compound:0259-0544|FD-Score:3.96|P-value:1.50E-4 ID:SGTC_1516|Compound:st013856|FD-Score:4.97|P-value:2.83E-6 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:12.80|P-value:1.27E-31 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.20|P-value:6.19E-5 ID:SGTC_1602|Compound:st001878|FD-Score:-3.55|P-value:6.52E-4 ID:SGTC_1607|Compound:st000305|FD-Score:4.04|P-value:1.15E-4 ID:SGTC_1804|Compound:st050076|FD-Score:3.99|P-value:1.36E-4 ID:SGTC_1807|Compound:st050092|FD-Score:5.40|P-value:4.26E-7 ID:SGTC_1881|Compound:5652484|FD-Score:4.32|P-value:4.02E-5 ID:SGTC_2010|Compound:4012422|FD-Score:4.44|P-value:2.56E-5 ID:SGTC_2029|Compound:5175081|FD-Score:5.37|P-value:4.72E-7 ID:SGTC_2057|Compound:5236571|FD-Score:4.02|P-value:1.19E-4 ID:SGTC_2220|Compound:6782180|FD-Score:4.32|P-value:4.10E-5 ID:SGTC_2437|Compound:5741531|FD-Score:-3.69|P-value:4.04E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:5.26|P-value:8.12E-7 ID:SGTC_2619|Compound:obliquin|FD-Score:4.44|P-value:2.58E-5 ID:SGTC_2710|Compound:st078562|FD-Score:4.29|P-value:4.58E-5 ID:SGTC_2729|Compound:fluphenazine|FD-Score:4.74|P-value:7.62E-6 ID:SGTC_2814|Compound:7977482|FD-Score:4.41|P-value:2.81E-5 ID:SGTC_2957|Compound:9076800|FD-Score:5.87|P-value:4.27E-8 ID:SGTC_3012|Compound:9080433|FD-Score:4.20|P-value:6.31E-5 ID:SGTC_3259|Compound:9138485|FD-Score:-3.42|P-value:9.57E-4 ID:SGTC_3334|Compound:9142812|FD-Score:3.80|P-value:2.60E-4 ID:SGTC_3337|Compound:9144342|FD-Score:3.45|P-value:8.22E-4 ID:SGTC_3338|Compound:9144300|FD-Score:4.41|P-value:2.86E-5

Top fitness defect scores for YAR002W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1528 arabinosylhypoxanthine 12.80 74.6 µM 1.27E-31 1 1.07E-37
2 SGTC_965 1291-5240 7.24 308.0 µM 2.02E-11 2 2.20E-13
3 SGTC_1454 4239-0054 6.18 42.6 µM 8.67E-9 3 3.19E-10
4 SGTC_2957 9076800 5.87 11.5 µM 4.27E-8 6 2.16E-9
5 SGTC_1807 st050092 5.40 58.9 µM 4.26E-7 60S ribosome export 2 3.39E-8
6 SGTC_2029 5175081 5.37 200.0 µM 4.72E-7 2 3.84E-8
7 SGTC_528 3492-0019 5.29 172.0 µM 6.89E-7 calcium & mitochondrial duress 146 6.03E-8
8 SGTC_2584 androsterone 5.26 48.7 µM 8.12E-7 cell wall 74 7.34E-8
9 SGTC_1516 st013856 4.97 110.0 µM 2.83E-6 3 3.28E-7
10 SGTC_1136 pergolide 4.83 1.1 µM 5.24E-6 17 6.83E-7
11 SGTC_512 prima-1 4.81 270.0 µM 5.59E-6 DNA intercalators 11 7.38E-7
12 SGTC_2729 fluphenazine 4.74 10.7 µM 7.62E-6 3 1.07E-6
13 SGTC_745 0469-0705 4.56 296.0 µM 1.55E-5 mitochondrial processes 11 2.50E-6
14 SGTC_2010 4012422 4.44 159.0 µM 2.56E-5 14 4.55E-6
15 SGTC_2619 obliquin 4.44 87.9 µM 2.58E-5 cell wall 122 4.58E-6
16 SGTC_458 nppb 4.42 133.0 µM 2.76E-5 9 4.98E-6
17 SGTC_2814 7977482 4.41 43.0 µM 2.81E-5 35 5.08E-6
18 SGTC_3338 9144300 4.41 39.6 µM 2.86E-5 Golgi 22 5.20E-6
19 SGTC_742 0kpi-0133 4.34 140.0 µM 3.77E-5 ERG2 17 7.22E-6
20 SGTC_1881 5652484 4.32 16.0 µM 4.02E-5 TRP & mitochondrial translation 22 7.80E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.173 7.05E-24 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.146 2.17E-17 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.134 7.02E-15 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.115 2.80E-11 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.108 2.95E-10 YNL043C_d YNL043C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W
0.106 7.10E-10 YLR093C NYV1 hom v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane
0.103 2.43E-9 YHL014C YLF2 hom Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.100 6.93E-9 YOL016C CMK2 hom Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication
0.096 2.72E-8 YNL070W TOM7 hom Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex
0.093 6.95E-8 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.091 1.46E-7 YJL164C TPK1 hom cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
0.090 1.53E-7 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.089 2.26E-7 YGL203C KEX1 hom Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
0.088 3.23E-7 YGR253C PUP2 het Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
0.088 3.44E-7 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria