YAR007C / RFA1

Subunit of heterotrimeric Replication Protein A (RPA), which is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination

Zygosity: Heterozygous strain
fixedexpanded
Profile for YAR007C / RFA1

Click on Significant Values for Screen Details ID:SGTC_85|Compound:0868-0259|FD-Score:-4.69|P-value:1.34E-5 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:3.79|P-value:4.20E-4 ID:SGTC_289|Compound:k017-0060|FD-Score:3.97|P-value:2.27E-4 ID:SGTC_305|Compound:1682-2595|FD-Score:3.77|P-value:4.46E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:3.85|P-value:3.45E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:-4.11|P-value:1.16E-4 ID:SGTC_681|Compound:1642-0006|FD-Score:-3.58|P-value:6.67E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:-3.64|P-value:5.63E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-11.40|P-value:1.70E-24 ID:SGTC_1266|Compound:0828-0227|FD-Score:-3.93|P-value:2.19E-4 ID:SGTC_1334|Compound:1486-0610|FD-Score:-3.65|P-value:5.35E-4 ID:SGTC_1805|Compound:st050013|FD-Score:-3.69|P-value:4.66E-4 ID:SGTC_1938|Compound:st074717|FD-Score:-4.21|P-value:8.10E-5 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:-3.77|P-value:3.69E-4 ID:SGTC_2176|Compound:5809774|FD-Score:-4.87|P-value:6.69E-6 ID:SGTC_2178|Compound:5812956|FD-Score:3.66|P-value:6.15E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.63|P-value:6.94E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:-3.54|P-value:7.66E-4 ID:SGTC_2837|Compound:9008476|FD-Score:3.86|P-value:3.33E-4 ID:SGTC_2873|Compound:9040596|FD-Score:4.41|P-value:4.94E-5 ID:SGTC_2944|Compound:9067346|FD-Score:4.75|P-value:1.36E-5 ID:SGTC_2946|Compound:9065502|FD-Score:-4.51|P-value:2.72E-5 ID:SGTC_2969|Compound:9091408|FD-Score:3.62|P-value:7.01E-4 ID:SGTC_2976|Compound:9009005|FD-Score:-3.54|P-value:7.47E-4 ID:SGTC_3108|Compound:9121785|FD-Score:3.58|P-value:8.01E-4 ID:SGTC_3245|Compound:9134821|FD-Score:4.53|P-value:3.15E-5 ID:SGTC_3247|Compound:9135149|FD-Score:3.68|P-value:5.95E-4 ID:SGTC_85|Compound:0868-0259|FD-Score:-4.69|P-value:1.34E-5 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:3.79|P-value:4.20E-4 ID:SGTC_289|Compound:k017-0060|FD-Score:3.97|P-value:2.27E-4 ID:SGTC_305|Compound:1682-2595|FD-Score:3.77|P-value:4.46E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:3.85|P-value:3.45E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:-4.11|P-value:1.16E-4 ID:SGTC_681|Compound:1642-0006|FD-Score:-3.58|P-value:6.67E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:-3.64|P-value:5.63E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-11.40|P-value:1.70E-24 ID:SGTC_1266|Compound:0828-0227|FD-Score:-3.93|P-value:2.19E-4 ID:SGTC_1334|Compound:1486-0610|FD-Score:-3.65|P-value:5.35E-4 ID:SGTC_1805|Compound:st050013|FD-Score:-3.69|P-value:4.66E-4 ID:SGTC_1938|Compound:st074717|FD-Score:-4.21|P-value:8.10E-5 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:-3.77|P-value:3.69E-4 ID:SGTC_2176|Compound:5809774|FD-Score:-4.87|P-value:6.69E-6 ID:SGTC_2178|Compound:5812956|FD-Score:3.66|P-value:6.15E-4 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.63|P-value:6.94E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:-3.54|P-value:7.66E-4 ID:SGTC_2837|Compound:9008476|FD-Score:3.86|P-value:3.33E-4 ID:SGTC_2873|Compound:9040596|FD-Score:4.41|P-value:4.94E-5 ID:SGTC_2944|Compound:9067346|FD-Score:4.75|P-value:1.36E-5 ID:SGTC_2946|Compound:9065502|FD-Score:-4.51|P-value:2.72E-5 ID:SGTC_2969|Compound:9091408|FD-Score:3.62|P-value:7.01E-4 ID:SGTC_2976|Compound:9009005|FD-Score:-3.54|P-value:7.47E-4 ID:SGTC_3108|Compound:9121785|FD-Score:3.58|P-value:8.01E-4 ID:SGTC_3245|Compound:9134821|FD-Score:4.53|P-value:3.15E-5 ID:SGTC_3247|Compound:9135149|FD-Score:3.68|P-value:5.95E-4

Top fitness defect scores for YAR007C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2944 9067346 4.75 25.6 µM 1.36E-5 6 1.02E-6 0.13
2 SGTC_3245 9134821 4.53 49.5 µM 3.15E-5 1 2.94E-6 0.53
3 SGTC_2873 9040596 4.41 71.4 µM 4.94E-5 4 5.19E-6 0.60
4 SGTC_289 k017-0060 3.97 526.0 µM 2.27E-4 1 3.54E-5 0.50
5 SGTC_2837 9008476 3.86 45.5 µM 3.33E-4 7 5.72E-5 0.20
6 SGTC_627 1391-0720 3.85 175.0 µM 3.45E-4 3 5.99E-5 0.17
7 SGTC_240 5hr heat shock (37°C) + Streptovitacin 3.79 300.0 µM 4.20E-4 heat shock/prefoldin 2 7.68E-5 0.13
8 SGTC_305 1682-2595 3.77 20.8 µM 4.46E-4 RNA processing & uracil transport 9 8.26E-5 0.00
9 SGTC_3247 9135149 3.68 49.5 µM 5.95E-4 DNA damage response 25 1.19E-4 0.02
10 SGTC_2178 5812956 3.66 200.0 µM 6.15E-4 6 1.24E-4 0.43
11 SGTC_2500 5-alpha-androstan-3,17-dione 3.63 61.4 µM 6.94E-4 5 1.44E-4 0.03
12 SGTC_2969 9091408 3.62 28.7 µM 7.01E-4 10 1.46E-4 0.00
13 SGTC_3108 9121785 3.58 49.5 µM 8.01E-4 17 1.73E-4 0.03
14 SGTC_2502 avocadyne acetate 3.43 18.6 µM 0.00127 8 3.07E-4 0.24
15 SGTC_637 1181-0519 3.41 7.3 µM 0.00131 fatty acid desaturase (OLE1) 16 3.19E-4 0.05
16 SGTC_1058 3448-5425 3.38 97.3 µM 0.00145 16 3.63E-4 0.01
17 SGTC_2007 4011539 3.37 71.8 µM 0.00151 cell wall signaling 12 3.82E-4 0.09
18 SGTC_1381 2324-0066 3.24 62.1 µM 0.00217 14 6.02E-4 0.02
19 SGTC_1396 3511-0007 3.23 98.6 µM 0.00221 mitochondrial processes 8 6.17E-4 0.03
20 SGTC_1056 3448-1962 3.23 115.0 µM 0.00223 amide catabolism 11 6.25E-4 0.11

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.210 9.94E-35 YPR088C SRP54 het Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain
0.182 2.68E-26 YML025C YML6 het Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
0.144 4.57E-17 YOR299W BUD7 hom Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication
0.120 3.00E-12 YDR074W TPS2 hom Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress
0.117 1.01E-11 YNL026W SAM50 het Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85
0.117 1.09E-11 YKL080W VMA5 hom Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane
0.112 9.55E-11 YGL152C_d YGL152C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PEX14/YGL153W
0.110 1.69E-10 YMR301C ATM1 het Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol
0.107 5.10E-10 YOR151C RPB2 het RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit
0.104 1.60E-9 YOR270C VPH1 hom Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress
0.104 1.66E-9 YLR262C-A TMA7 hom Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress
0.101 5.27E-9 YGR036C CAX4 hom Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation
0.099 9.02E-9 YAL066W_d YAL066W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.097 1.93E-8 YCR091W KIN82 hom Putative serine/threonine protein kinase implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases) Lem3p-Dnf1p/Dnf2p; similar to Fpk1p
0.096 2.79E-8 YOR222W ODC2 hom Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism