YAR015W / ADE1

N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YAR015W / ADE1

Click on Significant Values for Screen Details ID:SGTC_93|Compound:0109-0045|FD-Score:3.23|P-value:5.87E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:3.52|P-value:2.05E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:3.75|P-value:8.24E-5 ID:SGTC_360|Compound:0957-0399|FD-Score:4.13|P-value:1.70E-5 ID:SGTC_584|Compound:0986-0246|FD-Score:3.26|P-value:5.33E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:-3.65|P-value:1.30E-4 ID:SGTC_876|Compound:0720-0963|FD-Score:-4.20|P-value:1.34E-5 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.29|P-value:4.95E-4 ID:SGTC_1021|Compound:4476-0760|FD-Score:-3.09|P-value:9.96E-4 ID:SGTC_1024|Compound:4507-0395|FD-Score:-5.47|P-value:2.15E-8 ID:SGTC_1173|Compound:0443-0020|FD-Score:3.15|P-value:7.72E-4 ID:SGTC_1175|Compound:0741-0056|FD-Score:3.33|P-value:4.07E-4 ID:SGTC_1186|Compound:1319-0216|FD-Score:3.20|P-value:6.63E-4 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.21|P-value:6.23E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:4.76|P-value:8.60E-7 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.66|P-value:1.19E-4 ID:SGTC_1295|Compound:1013-0063|FD-Score:4.75|P-value:9.28E-7 ID:SGTC_1318|Compound:1280-3875|FD-Score:4.70|P-value:1.19E-6 ID:SGTC_1323|Compound:1348-1351|FD-Score:3.92|P-value:4.06E-5 ID:SGTC_1386|Compound:3228-0095|FD-Score:3.18|P-value:7.00E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:4.14|P-value:1.61E-5 ID:SGTC_1558|Compound:st069300|FD-Score:3.27|P-value:5.10E-4 ID:SGTC_1685|Compound:st024049|FD-Score:3.28|P-value:4.90E-4 ID:SGTC_1701|Compound:st028638|FD-Score:-3.14|P-value:8.52E-4 ID:SGTC_1705|Compound:st027868|FD-Score:3.11|P-value:8.95E-4 ID:SGTC_1726|Compound:st035556|FD-Score:3.77|P-value:7.58E-5 ID:SGTC_1766|Compound:st045414|FD-Score:3.72|P-value:9.16E-5 ID:SGTC_1834|Compound:st055981|FD-Score:3.84|P-value:5.74E-5 ID:SGTC_1901|Compound:st060216|FD-Score:3.30|P-value:4.54E-4 ID:SGTC_1923|Compound:st058458|FD-Score:4.94|P-value:3.45E-7 ID:SGTC_1983|Compound:st071813|FD-Score:3.08|P-value:9.90E-4 ID:SGTC_2013|Compound:4024628|FD-Score:4.13|P-value:1.65E-5 ID:SGTC_2104|Compound:5389443|FD-Score:3.16|P-value:7.56E-4 ID:SGTC_2124|Compound:5246320|FD-Score:3.93|P-value:3.96E-5 ID:SGTC_2174|Compound:5790901|FD-Score:4.59|P-value:2.04E-6 ID:SGTC_2180|Compound:6301957|FD-Score:-3.47|P-value:2.58E-4 ID:SGTC_2204|Compound:7233102|FD-Score:3.77|P-value:7.79E-5 ID:SGTC_2263|Compound:7948595|FD-Score:3.34|P-value:3.96E-4 ID:SGTC_2282|Compound:7944268|FD-Score:3.57|P-value:1.70E-4 ID:SGTC_2361|Compound:9034121|FD-Score:3.52|P-value:2.01E-4 ID:SGTC_2399|Compound:5884133|FD-Score:3.85|P-value:5.49E-5 ID:SGTC_2402|Compound:5553605|FD-Score:3.47|P-value:2.44E-4 ID:SGTC_2462|Compound:5162487|FD-Score:3.43|P-value:2.82E-4 ID:SGTC_2511|Compound:berberine|FD-Score:-3.34|P-value:4.20E-4 ID:SGTC_2517|Compound:totarol|FD-Score:-3.92|P-value:4.29E-5 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:-3.98|P-value:3.42E-5 ID:SGTC_2581|Compound:avocatin a|FD-Score:3.71|P-value:9.68E-5 ID:SGTC_2591|Compound:skatole|FD-Score:4.76|P-value:8.99E-7 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:3.27|P-value:5.05E-4 ID:SGTC_2641|Compound:levomentholum|FD-Score:3.19|P-value:6.86E-4 ID:SGTC_2658|Compound:polymyxin b sulfate|FD-Score:3.27|P-value:5.12E-4 ID:SGTC_2742|Compound:clothiapine|FD-Score:3.38|P-value:3.39E-4 ID:SGTC_2792|Compound:7535235|FD-Score:4.11|P-value:1.83E-5 ID:SGTC_2857|Compound:9024691|FD-Score:3.17|P-value:7.26E-4 ID:SGTC_3126|Compound:9127922|FD-Score:5.16|P-value:1.10E-7 ID:SGTC_3277|Compound:9138724|FD-Score:4.62|P-value:1.71E-6 ID:SGTC_3278|Compound:9139605|FD-Score:3.80|P-value:6.63E-5 ID:SGTC_3289|Compound:9119912|FD-Score:4.37|P-value:5.71E-6 ID:SGTC_3297|Compound:9117502|FD-Score:3.10|P-value:9.31E-4 ID:SGTC_93|Compound:0109-0045|FD-Score:3.23|P-value:5.87E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:3.52|P-value:2.05E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:3.75|P-value:8.24E-5 ID:SGTC_360|Compound:0957-0399|FD-Score:4.13|P-value:1.70E-5 ID:SGTC_584|Compound:0986-0246|FD-Score:3.26|P-value:5.33E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:-3.65|P-value:1.30E-4 ID:SGTC_876|Compound:0720-0963|FD-Score:-4.20|P-value:1.34E-5 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.29|P-value:4.95E-4 ID:SGTC_1021|Compound:4476-0760|FD-Score:-3.09|P-value:9.96E-4 ID:SGTC_1024|Compound:4507-0395|FD-Score:-5.47|P-value:2.15E-8 ID:SGTC_1173|Compound:0443-0020|FD-Score:3.15|P-value:7.72E-4 ID:SGTC_1175|Compound:0741-0056|FD-Score:3.33|P-value:4.07E-4 ID:SGTC_1186|Compound:1319-0216|FD-Score:3.20|P-value:6.63E-4 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.21|P-value:6.23E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:4.76|P-value:8.60E-7 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.66|P-value:1.19E-4 ID:SGTC_1295|Compound:1013-0063|FD-Score:4.75|P-value:9.28E-7 ID:SGTC_1318|Compound:1280-3875|FD-Score:4.70|P-value:1.19E-6 ID:SGTC_1323|Compound:1348-1351|FD-Score:3.92|P-value:4.06E-5 ID:SGTC_1386|Compound:3228-0095|FD-Score:3.18|P-value:7.00E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:4.14|P-value:1.61E-5 ID:SGTC_1558|Compound:st069300|FD-Score:3.27|P-value:5.10E-4 ID:SGTC_1685|Compound:st024049|FD-Score:3.28|P-value:4.90E-4 ID:SGTC_1701|Compound:st028638|FD-Score:-3.14|P-value:8.52E-4 ID:SGTC_1705|Compound:st027868|FD-Score:3.11|P-value:8.95E-4 ID:SGTC_1726|Compound:st035556|FD-Score:3.77|P-value:7.58E-5 ID:SGTC_1766|Compound:st045414|FD-Score:3.72|P-value:9.16E-5 ID:SGTC_1834|Compound:st055981|FD-Score:3.84|P-value:5.74E-5 ID:SGTC_1901|Compound:st060216|FD-Score:3.30|P-value:4.54E-4 ID:SGTC_1923|Compound:st058458|FD-Score:4.94|P-value:3.45E-7 ID:SGTC_1983|Compound:st071813|FD-Score:3.08|P-value:9.90E-4 ID:SGTC_2013|Compound:4024628|FD-Score:4.13|P-value:1.65E-5 ID:SGTC_2104|Compound:5389443|FD-Score:3.16|P-value:7.56E-4 ID:SGTC_2124|Compound:5246320|FD-Score:3.93|P-value:3.96E-5 ID:SGTC_2174|Compound:5790901|FD-Score:4.59|P-value:2.04E-6 ID:SGTC_2180|Compound:6301957|FD-Score:-3.47|P-value:2.58E-4 ID:SGTC_2204|Compound:7233102|FD-Score:3.77|P-value:7.79E-5 ID:SGTC_2263|Compound:7948595|FD-Score:3.34|P-value:3.96E-4 ID:SGTC_2282|Compound:7944268|FD-Score:3.57|P-value:1.70E-4 ID:SGTC_2361|Compound:9034121|FD-Score:3.52|P-value:2.01E-4 ID:SGTC_2399|Compound:5884133|FD-Score:3.85|P-value:5.49E-5 ID:SGTC_2402|Compound:5553605|FD-Score:3.47|P-value:2.44E-4 ID:SGTC_2462|Compound:5162487|FD-Score:3.43|P-value:2.82E-4 ID:SGTC_2511|Compound:berberine|FD-Score:-3.34|P-value:4.20E-4 ID:SGTC_2517|Compound:totarol|FD-Score:-3.92|P-value:4.29E-5 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:-3.98|P-value:3.42E-5 ID:SGTC_2581|Compound:avocatin a|FD-Score:3.71|P-value:9.68E-5 ID:SGTC_2591|Compound:skatole|FD-Score:4.76|P-value:8.99E-7 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:3.27|P-value:5.05E-4 ID:SGTC_2641|Compound:levomentholum|FD-Score:3.19|P-value:6.86E-4 ID:SGTC_2658|Compound:polymyxin b sulfate|FD-Score:3.27|P-value:5.12E-4 ID:SGTC_2742|Compound:clothiapine|FD-Score:3.38|P-value:3.39E-4 ID:SGTC_2792|Compound:7535235|FD-Score:4.11|P-value:1.83E-5 ID:SGTC_2857|Compound:9024691|FD-Score:3.17|P-value:7.26E-4 ID:SGTC_3126|Compound:9127922|FD-Score:5.16|P-value:1.10E-7 ID:SGTC_3277|Compound:9138724|FD-Score:4.62|P-value:1.71E-6 ID:SGTC_3278|Compound:9139605|FD-Score:3.80|P-value:6.63E-5 ID:SGTC_3289|Compound:9119912|FD-Score:4.37|P-value:5.71E-6 ID:SGTC_3297|Compound:9117502|FD-Score:3.10|P-value:9.31E-4

Top fitness defect scores for YAR015W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3126 9127922 5.16 49.5 µM 1.10E-7 fatty acid desaturase (OLE1) 21 1.23E-7
2 SGTC_1923 st058458 4.94 19.1 µM 3.45E-7 15 3.81E-7
3 SGTC_1214 0358-0031 4.76 63.7 µM 8.60E-7 5 9.46E-7
4 SGTC_2591 skatole 4.76 100.0 µM 8.99E-7 16 9.88E-7
5 SGTC_1295 1013-0063 4.75 9.8 µM 9.28E-7 2 1.02E-6
6 SGTC_1318 1280-3875 4.70 124.0 µM 1.19E-6 15 1.31E-6
7 SGTC_3277 9138724 4.62 49.5 µM 1.71E-6 Golgi 14 1.88E-6
8 SGTC_2174 5790901 4.59 200.0 µM 2.04E-6 tubulin folding & SWR complex 28 2.23E-6
9 SGTC_3289 9119912 4.37 71.4 µM 5.71E-6 10 6.20E-6
10 SGTC_1465 k213-0056 4.14 144.0 µM 1.61E-5 DNA intercalators 19 1.74E-5
11 SGTC_2013 4024628 4.13 200.0 µM 1.65E-5 37 1.78E-5
12 SGTC_360 0957-0399 4.13 1.0 µM 1.70E-5 cell wall 39 1.83E-5
13 SGTC_2792 7535235 4.11 81.8 µM 1.83E-5 fatty acid desaturase (OLE1) 29 1.98E-5
14 SGTC_2124 5246320 3.93 200.0 µM 3.96E-5 heme biosynthesis & mitochondrial translocase 20 4.25E-5
15 SGTC_1323 1348-1351 3.92 11.2 µM 4.06E-5 7 4.35E-5
16 SGTC_2399 5884133 3.85 200.0 µM 5.49E-5 cell wall signaling 75 5.88E-5
17 SGTC_1834 st055981 3.84 52.8 µM 5.74E-5 RPP1 & pyrimidine depletion 37 6.15E-5
18 SGTC_3278 9139605 3.80 49.5 µM 6.63E-5 19 7.09E-5
19 SGTC_1726 st035556 3.77 14.6 µM 7.58E-5 39 8.10E-5
20 SGTC_2204 7233102 3.77 200.0 µM 7.79E-5 60S ribosome export 4 8.33E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.078 5.62E-6 YGR204W ADE3 hom Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
0.076 9.71E-6 YNL236W SIN4 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
0.075 1.20E-5 YGR087C PDC6 hom Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation
0.072 2.98E-5 YMR094W CTF13 het Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis
0.072 3.10E-5 YLR178C TFS1 hom Protein that interacts with and inhibits carboxypeptidase Y and Ira2p; phosphatidylethanolamine-binding protein (PEBP) family member; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress
0.071 3.63E-5 YPL237W SUI3 het Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
0.069 6.33E-5 YHR164C DNA2 het Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress
0.069 6.61E-5 YDL004W ATP16 het Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
0.068 7.92E-5 YCR069W CPR4 hom Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway
0.068 8.83E-5 YLR163C MAS1 het Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
0.067 1.00E-4 YKL091C YKL091C hom Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study
0.067 1.06E-4 YML041C VPS71 hom Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
0.067 1.07E-4 YMR279C YMR279C hom Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication
0.067 1.11E-4 YLL023C POM33 hom Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress
0.066 1.23E-4 YOR192C THI72 hom Transporter of thiamine or related compound; shares sequence similarity with Thi7p