YBL020W / RFT1

Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBL020W / RFT1

Click on Significant Values for Screen Details ID:SGTC_65|Compound:0798-0905|FD-Score:4.78|P-value:7.46E-6 ID:SGTC_132|Compound:0417-1715|FD-Score:3.77|P-value:3.07E-4 ID:SGTC_133|Compound:0724-0033|FD-Score:6.09|P-value:1.77E-8 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.48|P-value:9.15E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:4.13|P-value:8.96E-5 ID:SGTC_669|Compound:0108-0021|FD-Score:-3.61|P-value:6.12E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:-4.03|P-value:1.52E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:-3.64|P-value:5.55E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:4.08|P-value:1.08E-4 ID:SGTC_2029|Compound:5175081|FD-Score:3.63|P-value:4.87E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.68|P-value:4.85E-4 ID:SGTC_2413|Compound:inermin|FD-Score:3.91|P-value:1.92E-4 ID:SGTC_2525|Compound:harmine|FD-Score:-3.66|P-value:5.20E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.76|P-value:3.23E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.44|P-value:8.77E-4 ID:SGTC_2805|Compound:7950783|FD-Score:3.83|P-value:2.51E-4 ID:SGTC_2813|Compound:7971799|FD-Score:5.33|P-value:6.92E-7 ID:SGTC_2815|Compound:7968870|FD-Score:3.76|P-value:3.21E-4 ID:SGTC_2892|Compound:9047358|FD-Score:3.47|P-value:8.10E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-5.15|P-value:1.85E-6 ID:SGTC_3054|Compound:9094397|FD-Score:4.35|P-value:4.01E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-8.60|P-value:5.09E-15 ID:SGTC_3069|Compound:9117009|FD-Score:-3.51|P-value:8.33E-4 ID:SGTC_3283|Compound:9109332|FD-Score:-4.74|P-value:1.05E-5 ID:SGTC_3293|Compound:9120939|FD-Score:5.20|P-value:1.24E-6 ID:SGTC_65|Compound:0798-0905|FD-Score:4.78|P-value:7.46E-6 ID:SGTC_132|Compound:0417-1715|FD-Score:3.77|P-value:3.07E-4 ID:SGTC_133|Compound:0724-0033|FD-Score:6.09|P-value:1.77E-8 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.48|P-value:9.15E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:4.13|P-value:8.96E-5 ID:SGTC_669|Compound:0108-0021|FD-Score:-3.61|P-value:6.12E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:-4.03|P-value:1.52E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:-3.64|P-value:5.55E-4 ID:SGTC_1255|Compound:0566-0045|FD-Score:4.08|P-value:1.08E-4 ID:SGTC_2029|Compound:5175081|FD-Score:3.63|P-value:4.87E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.68|P-value:4.85E-4 ID:SGTC_2413|Compound:inermin|FD-Score:3.91|P-value:1.92E-4 ID:SGTC_2525|Compound:harmine|FD-Score:-3.66|P-value:5.20E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.76|P-value:3.23E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.44|P-value:8.77E-4 ID:SGTC_2805|Compound:7950783|FD-Score:3.83|P-value:2.51E-4 ID:SGTC_2813|Compound:7971799|FD-Score:5.33|P-value:6.92E-7 ID:SGTC_2815|Compound:7968870|FD-Score:3.76|P-value:3.21E-4 ID:SGTC_2892|Compound:9047358|FD-Score:3.47|P-value:8.10E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-5.15|P-value:1.85E-6 ID:SGTC_3054|Compound:9094397|FD-Score:4.35|P-value:4.01E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-8.60|P-value:5.09E-15 ID:SGTC_3069|Compound:9117009|FD-Score:-3.51|P-value:8.33E-4 ID:SGTC_3283|Compound:9109332|FD-Score:-4.74|P-value:1.05E-5 ID:SGTC_3293|Compound:9120939|FD-Score:5.20|P-value:1.24E-6

Top fitness defect scores for YBL020W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_133 0724-0033 6.09 378.9 µM 1.77E-8 3 5.74E-10 0.78
2 SGTC_2813 7971799 5.33 71.4 µM 6.92E-7 3 4.98E-8 0.99
3 SGTC_3293 9120939 5.20 62.0 µM 1.24E-6 2 1.01E-7 0.47
4 SGTC_65 0798-0905 4.78 35.0 µM 7.46E-6 calcium & mitochondrial duress 4 8.95E-7 0.13
5 SGTC_3054 9094397 4.35 49.5 µM 4.01E-5 fatty acid desaturase (OLE1) 5 6.88E-6 0.12
6 SGTC_611 3453-1652 4.13 23.7 µM 8.96E-5 19 1.82E-5 0.01
7 SGTC_1255 0566-0045 4.08 221.0 µM 1.08E-4 excess fatty acid 14 2.27E-5 0.14
8 SGTC_2413 inermin 3.91 26.1 µM 1.92E-4 17 4.57E-5 0.01
9 SGTC_2805 7950783 3.83 58.4 µM 2.51E-4 9 6.32E-5 0.17
10 SGTC_132 0417-1715 3.77 25.8 µM 3.07E-4 RPP1 & pyrimidine depletion 11 8.07E-5 0.39
11 SGTC_2815 7968870 3.76 71.4 µM 3.21E-4 7 8.49E-5 0.17
12 SGTC_2568 diosmetin 3.76 100.0 µM 3.23E-4 12 8.56E-5 6.03E-4
13 SGTC_2029 5175081 3.63 200.0 µM 4.87E-4 9 1.41E-4 0.09
14 SGTC_2892 9047358 3.47 58.4 µM 8.10E-4 4 2.60E-4 0.00
15 SGTC_2592 piperlongumine 3.44 83.3 µM 8.77E-4 TSC3-RPN4 12 2.86E-4 0.04
16 SGTC_1901 st060216 3.37 58.6 µM 0.00110 7 3.77E-4 0.01
17 SGTC_1735 st034310 3.33 56.8 µM 0.00124 Golgi 16 4.32E-4 0.06
18 SGTC_972 1319-0219 3.31 195.0 µM 0.00134 12 4.75E-4 0.02
19 SGTC_2683 piperine 3.23 63.0 µM 0.00166 4 6.15E-4 0.04
20 SGTC_3189 9110682 3.21 49.5 µM 0.00176 15 6.59E-4 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.179 1.20E-25 YCR013C_d YCR013C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant
0.165 5.83E-22 YMR270C RRN9 het Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I
0.150 2.81E-18 YIL147C SLN1 het Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators
0.149 3.21E-18 YAR008W SEN34 het Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
0.149 4.79E-18 YER018C SPC25 het Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
0.146 1.83E-17 YDL007W RPT2 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle
0.143 1.01E-16 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.141 2.19E-16 YBL004W UTP20 het Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
0.141 2.86E-16 YDR373W FRQ1 het N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
0.130 4.52E-14 YFL038C YPT1 het Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
0.126 2.57E-13 YML046W PRP39 het U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats
0.123 7.09E-13 YGL142C GPI10 het Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog
0.122 1.12E-12 YBR193C MED8 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.121 1.93E-12 YKL083W_d YKL083W_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
0.121 2.14E-12 YOR335C ALA1 het Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog