YBL021C / HAP3

Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding

Zygosity: Homozygous strain
fixedexpanded
Profile for YBL021C / HAP3

Click on Significant Values for Screen Details ID:SGTC_115|Compound:0527-0192|FD-Score:-3.16|P-value:8.18E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:-3.64|P-value:1.46E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:3.47|P-value:3.18E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:14.70|P-value:6.52E-48 ID:SGTC_310|Compound:1273-0058|FD-Score:5.78|P-value:5.98E-9 ID:SGTC_356|Compound:1634-0012|FD-Score:-3.71|P-value:1.09E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:3.65|P-value:1.67E-4 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:-3.12|P-value:9.26E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:3.24|P-value:7.30E-4 ID:SGTC_640|Compound:3381-0960|FD-Score:4.23|P-value:1.55E-5 ID:SGTC_822|Compound:1487-0950|FD-Score:-3.61|P-value:1.60E-4 ID:SGTC_945|Compound:3381-0768|FD-Score:-4.15|P-value:1.77E-5 ID:SGTC_993|Compound:3909-8757|FD-Score:3.35|P-value:4.99E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:-3.27|P-value:5.48E-4 ID:SGTC_1610|Compound:st001799|FD-Score:-3.94|P-value:4.33E-5 ID:SGTC_1615|Compound:st002656|FD-Score:-3.98|P-value:3.66E-5 ID:SGTC_1635|Compound:st007035|FD-Score:10.00|P-value:2.13E-23 ID:SGTC_1738|Compound:st035555|FD-Score:-3.52|P-value:2.29E-4 ID:SGTC_2074|Compound:5212007|FD-Score:4.68|P-value:2.01E-6 ID:SGTC_2077|Compound:5212015|FD-Score:-3.30|P-value:5.02E-4 ID:SGTC_2326|Compound:6164455|FD-Score:3.66|P-value:1.57E-4 ID:SGTC_2364|Compound:9055074|FD-Score:4.56|P-value:3.46E-6 ID:SGTC_2762|Compound:leflunomide|FD-Score:3.21|P-value:7.92E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.72|P-value:1.25E-4 ID:SGTC_2946|Compound:9065502|FD-Score:-4.54|P-value:3.10E-6 ID:SGTC_3090|Compound:9117696|FD-Score:-3.39|P-value:3.67E-4 ID:SGTC_3125|Compound:9127534|FD-Score:-3.95|P-value:4.23E-5 ID:SGTC_3135|Compound:9127742|FD-Score:3.20|P-value:8.25E-4 ID:SGTC_3137|Compound:9097352|FD-Score:-3.36|P-value:4.02E-4 ID:SGTC_3145|Compound:9085516|FD-Score:4.61|P-value:2.73E-6 ID:SGTC_115|Compound:0527-0192|FD-Score:-3.16|P-value:8.18E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:-3.64|P-value:1.46E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:3.47|P-value:3.18E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:14.70|P-value:6.52E-48 ID:SGTC_310|Compound:1273-0058|FD-Score:5.78|P-value:5.98E-9 ID:SGTC_356|Compound:1634-0012|FD-Score:-3.71|P-value:1.09E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:3.65|P-value:1.67E-4 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:-3.12|P-value:9.26E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:3.24|P-value:7.30E-4 ID:SGTC_640|Compound:3381-0960|FD-Score:4.23|P-value:1.55E-5 ID:SGTC_822|Compound:1487-0950|FD-Score:-3.61|P-value:1.60E-4 ID:SGTC_945|Compound:3381-0768|FD-Score:-4.15|P-value:1.77E-5 ID:SGTC_993|Compound:3909-8757|FD-Score:3.35|P-value:4.99E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:-3.27|P-value:5.48E-4 ID:SGTC_1610|Compound:st001799|FD-Score:-3.94|P-value:4.33E-5 ID:SGTC_1615|Compound:st002656|FD-Score:-3.98|P-value:3.66E-5 ID:SGTC_1635|Compound:st007035|FD-Score:10.00|P-value:2.13E-23 ID:SGTC_1738|Compound:st035555|FD-Score:-3.52|P-value:2.29E-4 ID:SGTC_2074|Compound:5212007|FD-Score:4.68|P-value:2.01E-6 ID:SGTC_2077|Compound:5212015|FD-Score:-3.30|P-value:5.02E-4 ID:SGTC_2326|Compound:6164455|FD-Score:3.66|P-value:1.57E-4 ID:SGTC_2364|Compound:9055074|FD-Score:4.56|P-value:3.46E-6 ID:SGTC_2762|Compound:leflunomide|FD-Score:3.21|P-value:7.92E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.72|P-value:1.25E-4 ID:SGTC_2946|Compound:9065502|FD-Score:-4.54|P-value:3.10E-6 ID:SGTC_3090|Compound:9117696|FD-Score:-3.39|P-value:3.67E-4 ID:SGTC_3125|Compound:9127534|FD-Score:-3.95|P-value:4.23E-5 ID:SGTC_3135|Compound:9127742|FD-Score:3.20|P-value:8.25E-4 ID:SGTC_3137|Compound:9097352|FD-Score:-3.36|P-value:4.02E-4 ID:SGTC_3145|Compound:9085516|FD-Score:4.61|P-value:2.73E-6

Top fitness defect scores for YBL021C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_236 1509-0011 14.70 5.3 µM 6.52E-48 104 5.90E-49
2 SGTC_1635 st007035 10.00 42.4 µM 2.13E-23 74 6.42E-24
3 SGTC_310 1273-0058 5.78 1.1 µM 5.98E-9 mitochondrial stress 150 3.77E-9
4 SGTC_2074 5212007 4.68 75.4 µM 2.01E-6 6 1.45E-6
5 SGTC_3145 9085516 4.61 49.5 µM 2.73E-6 fatty acid desaturase (OLE1) 14 1.99E-6
6 SGTC_2364 9055074 4.56 200.0 µM 3.46E-6 12 2.53E-6
7 SGTC_640 3381-0960 4.23 37.1 µM 1.55E-5 7 1.17E-5
8 SGTC_2930 9013230 3.72 14.9 µM 1.25E-4 heme biosynthesis & mitochondrial translocase 48 9.95E-5
9 SGTC_2326 6164455 3.66 177.6 µM 1.57E-4 mitochondrial processes 32 1.26E-4
10 SGTC_386 1509-0011 3.65 3.1 µM 1.67E-4 161 1.34E-4
11 SGTC_157 1348-1695 3.47 65.8 µM 3.18E-4 iron homeostasis 102 2.59E-4
12 SGTC_993 3909-8757 3.35 62.7 µM 4.99E-4 44 4.11E-4
13 SGTC_533 1222-0222 3.24 6.7 µM 7.30E-4 PDR1 63 6.06E-4
14 SGTC_2762 leflunomide 3.21 42.1 µM 7.92E-4 61 6.59E-4
15 SGTC_3135 9127742 3.20 49.5 µM 8.25E-4 45 6.88E-4
16 SGTC_358 0075-0058 2.92 9.4 µM 0.00208 141 0.00177
17 SGTC_1733 st037357 2.88 54.9 µM 0.00230 60S ribosome export 110 0.00196
18 SGTC_352 k048-0007 2.81 5.1 µM 0.00290 ERAD & cell cycle 139 0.00249
19 SGTC_1756 st041558 2.76 35.5 µM 0.00331 45 0.00286
20 SGTC_299 1273-0059 2.75 1.4 µM 0.00348 78 0.00300

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.603 0 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.595 6.72E-320 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.586 4.24E-308 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.550 7.52E-265 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.547 2.69E-261 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.537 1.95E-250 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.533 5.32E-246 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.531 2.90E-243 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.531 2.27E-243 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.530 1.08E-242 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.525 1.50E-236 YOR065W CYT1 hom Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
0.524 2.30E-236 YPL132W COX11 hom Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
0.520 2.76E-232 YEL059C-A SOM1 hom Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.518 4.63E-230 YGR183C QCR9 hom Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
0.510 1.10E-221 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit