YBL024W / NCL1

S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120

Zygosity: Homozygous strain
fixedexpanded
Profile for YBL024W / NCL1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:4.06|P-value:2.42E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.74|P-value:6.43E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-4.17|P-value:1.55E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:3.68|P-value:7.75E-4 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.72|P-value:6.88E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:5.31|P-value:2.38E-6 ID:SGTC_629|Compound:3448-6466|FD-Score:-4.58|P-value:3.79E-5 ID:SGTC_631|Compound:k072-0230|FD-Score:-4.84|P-value:1.41E-5 ID:SGTC_633|Compound:0437-0093|FD-Score:-5.02|P-value:7.21E-6 ID:SGTC_634|Compound:1611-4317|FD-Score:3.62|P-value:9.28E-4 ID:SGTC_636|Compound:0kpi-0074|FD-Score:-3.89|P-value:3.87E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:-5.36|P-value:1.82E-6 ID:SGTC_690|Compound:glyoxal|FD-Score:13.30|P-value:1.57E-30 ID:SGTC_867|Compound:0518-0118|FD-Score:-4.42|P-value:6.55E-5 ID:SGTC_872|Compound:0588-0139|FD-Score:-3.65|P-value:7.87E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:-3.69|P-value:7.11E-4 ID:SGTC_1015|Compound:4130-4525|FD-Score:-4.13|P-value:1.76E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:-3.78|P-value:5.32E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.63|P-value:9.06E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.87|P-value:4.10E-4 ID:SGTC_1295|Compound:1013-0063|FD-Score:3.84|P-value:4.75E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:3.69|P-value:7.51E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.64|P-value:8.19E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.86|P-value:4.54E-4 ID:SGTC_1719|Compound:st032227|FD-Score:-9.23|P-value:7.80E-16 ID:SGTC_1723|Compound:st034307|FD-Score:-5.41|P-value:1.49E-6 ID:SGTC_1724|Compound:st037281|FD-Score:-4.49|P-value:5.27E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.72|P-value:6.92E-4 ID:SGTC_1875|Compound:nocodazole|FD-Score:12.20|P-value:5.53E-26 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.83|P-value:4.64E-4 ID:SGTC_2037|Compound:5169046|FD-Score:4.00|P-value:2.88E-4 ID:SGTC_2040|Compound:5100729|FD-Score:4.13|P-value:1.88E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.40|P-value:7.70E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:8.06|P-value:1.95E-12 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-4.00|P-value:2.70E-4 ID:SGTC_2871|Compound:9038510|FD-Score:3.73|P-value:6.74E-4 ID:SGTC_2927|Compound:9011802|FD-Score:3.83|P-value:4.90E-4 ID:SGTC_2934|Compound:9036370|FD-Score:4.24|P-value:1.31E-4 ID:SGTC_3210|Compound:9128157|FD-Score:4.32|P-value:1.01E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.06|P-value:2.42E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.74|P-value:6.43E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-4.17|P-value:1.55E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:3.68|P-value:7.75E-4 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.72|P-value:6.88E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:5.31|P-value:2.38E-6 ID:SGTC_629|Compound:3448-6466|FD-Score:-4.58|P-value:3.79E-5 ID:SGTC_631|Compound:k072-0230|FD-Score:-4.84|P-value:1.41E-5 ID:SGTC_633|Compound:0437-0093|FD-Score:-5.02|P-value:7.21E-6 ID:SGTC_634|Compound:1611-4317|FD-Score:3.62|P-value:9.28E-4 ID:SGTC_636|Compound:0kpi-0074|FD-Score:-3.89|P-value:3.87E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:-5.36|P-value:1.82E-6 ID:SGTC_690|Compound:glyoxal|FD-Score:13.30|P-value:1.57E-30 ID:SGTC_867|Compound:0518-0118|FD-Score:-4.42|P-value:6.55E-5 ID:SGTC_872|Compound:0588-0139|FD-Score:-3.65|P-value:7.87E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:-3.69|P-value:7.11E-4 ID:SGTC_1015|Compound:4130-4525|FD-Score:-4.13|P-value:1.76E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:-3.78|P-value:5.32E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.63|P-value:9.06E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.87|P-value:4.10E-4 ID:SGTC_1295|Compound:1013-0063|FD-Score:3.84|P-value:4.75E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:3.69|P-value:7.51E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.64|P-value:8.19E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.86|P-value:4.54E-4 ID:SGTC_1719|Compound:st032227|FD-Score:-9.23|P-value:7.80E-16 ID:SGTC_1723|Compound:st034307|FD-Score:-5.41|P-value:1.49E-6 ID:SGTC_1724|Compound:st037281|FD-Score:-4.49|P-value:5.27E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.72|P-value:6.92E-4 ID:SGTC_1875|Compound:nocodazole|FD-Score:12.20|P-value:5.53E-26 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.83|P-value:4.64E-4 ID:SGTC_2037|Compound:5169046|FD-Score:4.00|P-value:2.88E-4 ID:SGTC_2040|Compound:5100729|FD-Score:4.13|P-value:1.88E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.40|P-value:7.70E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:8.06|P-value:1.95E-12 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-4.00|P-value:2.70E-4 ID:SGTC_2871|Compound:9038510|FD-Score:3.73|P-value:6.74E-4 ID:SGTC_2927|Compound:9011802|FD-Score:3.83|P-value:4.90E-4 ID:SGTC_2934|Compound:9036370|FD-Score:4.24|P-value:1.31E-4 ID:SGTC_3210|Compound:9128157|FD-Score:4.32|P-value:1.01E-4

Top fitness defect scores for YBL024W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_690 glyoxal 13.30 20.3 µM 1.57E-30 8 2.12E-40
2 SGTC_1875 nocodazole 12.20 6.0 µM 5.53E-26 tubulin folding & SWR complex 10 2.69E-34
3 SGTC_2582 agaric acid 8.06 100.0 µM 1.95E-12 redox potentiating 103 3.94E-16
4 SGTC_451 chloral hydrate 5.31 100.0 µM 2.38E-6 21 5.37E-8
5 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 4.40 3.7 µM 7.70E-5 56 5.48E-6
6 SGTC_3210 9128157 4.32 49.5 µM 1.01E-4 38 7.84E-6
7 SGTC_2934 9036370 4.24 36.9 µM 1.31E-4 25 1.11E-5
8 SGTC_2040 5100729 4.13 123.0 µM 1.88E-4 39 1.79E-5
9 SGTC_58 0957-0393 4.06 9.0 µM 2.42E-4 cell wall 367 2.50E-5
10 SGTC_2037 5169046 4.00 200.0 µM 2.88E-4 60S ribosome export 14 3.15E-5
11 SGTC_1680 diphenhydramine 3.86 100.0 µM 4.54E-4 NEO1 31 5.76E-5
12 SGTC_1295 1013-0063 3.84 9.8 µM 4.75E-4 16 6.12E-5
13 SGTC_2927 9011802 3.83 47.9 µM 4.90E-4 28 6.38E-5
14 SGTC_159 3970-0959 3.74 50.0 µM 6.43E-4 87 9.13E-5
15 SGTC_2871 9038510 3.73 13.0 µM 6.74E-4 27 9.72E-5
16 SGTC_416 estradiol valerate 3.72 100.0 µM 6.88E-4 17 9.99E-5
17 SGTC_1844 rapamycin 3.72 4.0 nM 6.92E-4 179 1.01E-4
18 SGTC_1301 naphtanilide LB 3.69 4.8 µM 7.51E-4 heme requiring 15 1.12E-4
19 SGTC_299 1273-0059 3.68 1.4 µM 7.75E-4 30 1.17E-4
20 SGTC_1033 k072-0102 3.63 21.3 µM 9.06E-4 60S ribosome export 32 1.44E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.282 1.47E-62 YGL094C PAN2 hom Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
0.270 9.84E-57 YKL213C DOA1 hom WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress
0.202 4.17E-32 YGR190C_d YGR190C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
0.190 9.76E-29 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.176 7.19E-25 YLR113W HOG1 hom Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress
0.173 4.38E-24 YLR027C AAT2 hom Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
0.170 3.48E-23 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.170 3.92E-23 YJL110C GZF3 hom GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication
0.168 1.40E-22 YIL137C TMA108 hom Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease
0.167 1.67E-22 YGR032W GSC2 hom Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p)
0.167 2.57E-22 YNR031C SSK2 hom MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress
0.162 4.05E-21 YJL073W JEM1 hom DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2
0.160 1.16E-20 YDR049W VMS1 hom Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
0.158 4.23E-20 YKL025C PAN3 hom Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
0.156 1.20E-19 YOR364W_d YOR364W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C