YBL035C / POL12

B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBL035C / POL12

Click on Significant Values for Screen Details ID:SGTC_314|Compound:1671-0008|FD-Score:2.90|P-value:6.10E-4 ID:SGTC_531|Compound:3788-1495|FD-Score:3.00|P-value:4.17E-4 ID:SGTC_553|Compound:k007-0256|FD-Score:2.80|P-value:8.85E-4 ID:SGTC_632|Compound:0781-2353|FD-Score:2.88|P-value:6.60E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:2.92|P-value:5.68E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:5.33|P-value:1.87E-9 ID:SGTC_746|Compound:1095-0025|FD-Score:4.39|P-value:5.76E-7 ID:SGTC_820|Compound:1582-0060|FD-Score:-3.49|P-value:8.48E-5 ID:SGTC_829|Compound:1635-0120|FD-Score:2.91|P-value:5.82E-4 ID:SGTC_1027|Compound:k015-0025|FD-Score:3.14|P-value:2.29E-4 ID:SGTC_1030|Compound:k029-0042|FD-Score:3.36|P-value:9.28E-5 ID:SGTC_1049|Compound:3226-0134|FD-Score:-2.92|P-value:8.51E-4 ID:SGTC_1185|Compound:1319-0113|FD-Score:2.92|P-value:5.63E-4 ID:SGTC_1196|Compound:1588-0294|FD-Score:2.92|P-value:5.56E-4 ID:SGTC_1807|Compound:st050092|FD-Score:3.54|P-value:4.19E-5 ID:SGTC_1954|Compound:st077032|FD-Score:-3.77|P-value:2.36E-5 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:7.02|P-value:4.67E-15 ID:SGTC_2157|Compound:5649910|FD-Score:2.92|P-value:5.69E-4 ID:SGTC_2245|Compound:6637752|FD-Score:2.78|P-value:9.53E-4 ID:SGTC_2361|Compound:9034121|FD-Score:-3.04|P-value:5.36E-4 ID:SGTC_2416|Compound:tofa|FD-Score:-2.94|P-value:7.96E-4 ID:SGTC_2467|Compound:5474540|FD-Score:-5.69|P-value:3.37E-10 ID:SGTC_2472|Compound:5377652|FD-Score:3.12|P-value:2.55E-4 ID:SGTC_2478|Compound:5763493|FD-Score:3.13|P-value:2.41E-4 ID:SGTC_2488|Compound:5268135|FD-Score:3.48|P-value:5.42E-5 ID:SGTC_2644|Compound:guaiazulene|FD-Score:2.88|P-value:6.48E-4 ID:SGTC_2655|Compound:perillic acid|FD-Score:-4.22|P-value:2.55E-6 ID:SGTC_2676|Compound:harmalol|FD-Score:-3.24|P-value:2.40E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.60|P-value:5.23E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.00|P-value:4.06E-4 ID:SGTC_2936|Compound:9038235|FD-Score:-4.59|P-value:3.36E-7 ID:SGTC_2939|Compound:9044578|FD-Score:2.78|P-value:9.57E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.07|P-value:5.52E-6 ID:SGTC_3214|Compound:9129694|FD-Score:4.88|P-value:3.31E-8 ID:SGTC_3220|Compound:9131269|FD-Score:-3.31|P-value:1.84E-4 ID:SGTC_3221|Compound:9128209|FD-Score:-3.77|P-value:2.43E-5 ID:SGTC_3248|Compound:9135562|FD-Score:-2.96|P-value:7.26E-4 ID:SGTC_3324|Compound:9138759|FD-Score:-3.46|P-value:9.71E-5 ID:SGTC_3327|Compound:9140648|FD-Score:3.20|P-value:1.82E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:2.90|P-value:6.10E-4 ID:SGTC_531|Compound:3788-1495|FD-Score:3.00|P-value:4.17E-4 ID:SGTC_553|Compound:k007-0256|FD-Score:2.80|P-value:8.85E-4 ID:SGTC_632|Compound:0781-2353|FD-Score:2.88|P-value:6.60E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:2.92|P-value:5.68E-4 ID:SGTC_742|Compound:0kpi-0133|FD-Score:5.33|P-value:1.87E-9 ID:SGTC_746|Compound:1095-0025|FD-Score:4.39|P-value:5.76E-7 ID:SGTC_820|Compound:1582-0060|FD-Score:-3.49|P-value:8.48E-5 ID:SGTC_829|Compound:1635-0120|FD-Score:2.91|P-value:5.82E-4 ID:SGTC_1027|Compound:k015-0025|FD-Score:3.14|P-value:2.29E-4 ID:SGTC_1030|Compound:k029-0042|FD-Score:3.36|P-value:9.28E-5 ID:SGTC_1049|Compound:3226-0134|FD-Score:-2.92|P-value:8.51E-4 ID:SGTC_1185|Compound:1319-0113|FD-Score:2.92|P-value:5.63E-4 ID:SGTC_1196|Compound:1588-0294|FD-Score:2.92|P-value:5.56E-4 ID:SGTC_1807|Compound:st050092|FD-Score:3.54|P-value:4.19E-5 ID:SGTC_1954|Compound:st077032|FD-Score:-3.77|P-value:2.36E-5 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:7.02|P-value:4.67E-15 ID:SGTC_2157|Compound:5649910|FD-Score:2.92|P-value:5.69E-4 ID:SGTC_2245|Compound:6637752|FD-Score:2.78|P-value:9.53E-4 ID:SGTC_2361|Compound:9034121|FD-Score:-3.04|P-value:5.36E-4 ID:SGTC_2416|Compound:tofa|FD-Score:-2.94|P-value:7.96E-4 ID:SGTC_2467|Compound:5474540|FD-Score:-5.69|P-value:3.37E-10 ID:SGTC_2472|Compound:5377652|FD-Score:3.12|P-value:2.55E-4 ID:SGTC_2478|Compound:5763493|FD-Score:3.13|P-value:2.41E-4 ID:SGTC_2488|Compound:5268135|FD-Score:3.48|P-value:5.42E-5 ID:SGTC_2644|Compound:guaiazulene|FD-Score:2.88|P-value:6.48E-4 ID:SGTC_2655|Compound:perillic acid|FD-Score:-4.22|P-value:2.55E-6 ID:SGTC_2676|Compound:harmalol|FD-Score:-3.24|P-value:2.40E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.60|P-value:5.23E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.00|P-value:4.06E-4 ID:SGTC_2936|Compound:9038235|FD-Score:-4.59|P-value:3.36E-7 ID:SGTC_2939|Compound:9044578|FD-Score:2.78|P-value:9.57E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.07|P-value:5.52E-6 ID:SGTC_3214|Compound:9129694|FD-Score:4.88|P-value:3.31E-8 ID:SGTC_3220|Compound:9131269|FD-Score:-3.31|P-value:1.84E-4 ID:SGTC_3221|Compound:9128209|FD-Score:-3.77|P-value:2.43E-5 ID:SGTC_3248|Compound:9135562|FD-Score:-2.96|P-value:7.26E-4 ID:SGTC_3324|Compound:9138759|FD-Score:-3.46|P-value:9.71E-5 ID:SGTC_3327|Compound:9140648|FD-Score:3.20|P-value:1.82E-4

Top fitness defect scores for YBL035C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2042 p-Dimethylaminoacetophenone 7.02 200.0 µM 4.67E-15 1 1.11E-12 1.35
2 SGTC_742 0kpi-0133 5.33 140.0 µM 1.87E-9 ERG2 3 4.96E-8 1.16
3 SGTC_3214 9129694 4.88 49.5 µM 3.31E-8 3 5.38E-7 0.21
4 SGTC_746 1095-0025 4.39 46.6 µM 5.76E-7 RSC & ERG11 6 5.77E-6 0.02
5 SGTC_1807 st050092 3.54 58.9 µM 4.19E-5 60S ribosome export 7 2.03E-4 0.03
6 SGTC_2488 5268135 3.48 45.7 µM 5.42E-5 12 2.51E-4 0.04
7 SGTC_1030 k029-0042 3.36 36.7 µM 9.28E-5 TSC3-RPN4 9 3.93E-4 0.22
8 SGTC_3327 9140648 3.20 26.9 µM 1.82E-4 30 6.86E-4 0.07
9 SGTC_1027 k015-0025 3.14 8.7 µM 2.29E-4 11 8.31E-4 0.03
10 SGTC_2478 5763493 3.13 32.3 µM 2.41E-4 11 8.68E-4 0.03
11 SGTC_2472 5377652 3.12 49.4 µM 2.55E-4 TRP & mitochondrial translation 26 9.08E-4 0.04
12 SGTC_2778 tioconazole 3.00 65.1 nM 4.06E-4 ergosterol depletion effects on membrane 49 0.00134 0.05
13 SGTC_531 3788-1495 3.00 174.0 µM 4.17E-4 unfolded protein response 16 0.00137 0.03
14 SGTC_1196 1588-0294 2.92 184.0 µM 5.56E-4 11 0.00174 0.02
15 SGTC_1185 1319-0113 2.92 81.3 µM 5.63E-4 DNA damage response 12 0.00176 0.01
16 SGTC_701 4424-0219 2.92 185.0 µM 5.68E-4 calcium & mitochondrial duress 12 0.00177 0.05
17 SGTC_2157 5649910 2.92 96.4 µM 5.69E-4 20 0.00177 0.01
18 SGTC_829 1635-0120 2.91 117.0 µM 5.82E-4 DNA damage response 69 0.00181 0.07
19 SGTC_314 1671-0008 2.90 2.7 µM 6.10E-4 29 0.00188 0.03
20 SGTC_2644 guaiazulene 2.88 100.0 µM 6.48E-4 3 0.00197 0.17

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.127 1.71E-13 YNL115C_p YNL115C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene
0.122 1.55E-12 YDR046C BAP3 hom Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication
0.120 2.48E-12 YPL152W-A_p YPL152W-A_p hom Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.116 1.39E-11 YOL143C RIB4 hom Lumazine synthase (6,7-dimethyl-8-ribityllumazine synthase, also known as DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin
0.116 1.75E-11 YDR476C_p YDR476C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene
0.112 9.23E-11 YLR141W RRN5 het Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p
0.111 1.05E-10 YDR416W SYF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans
0.111 1.09E-10 YOL055C THI20 hom Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p
0.108 3.48E-10 YBR087W RFC5 het Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
0.104 1.35E-9 YDR352W YPQ2 hom Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; mutant phenotype is functionally complemented by rat PQLC2 vacuolar transporter
0.104 1.71E-9 YMR308C PSE1 het Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p
0.097 1.91E-8 YCR076C FUB1 hom Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene
0.095 3.39E-8 YDL222C FMP45 hom Integral membrane protein localized to mitochondria (untagged protein); required for sporulation and maintaining sphingolipid content; has sequence similarity to SUR7 and YNL194C
0.094 5.62E-8 YOL069W NUF2 het Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
0.092 1.03E-7 YNL321W VNX1 hom Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate