YBL039C / URA7

Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YBL039C / URA7

Click on Significant Values for Screen Details ID:SGTC_67|Compound:0335-0847|FD-Score:4.16|P-value:4.82E-5 ID:SGTC_70|Compound:0141-0289|FD-Score:3.29|P-value:9.93E-4 ID:SGTC_202|Compound:3594-0513|FD-Score:4.10|P-value:6.07E-5 ID:SGTC_212|Compound:3807-4682|FD-Score:4.18|P-value:4.45E-5 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:-3.47|P-value:8.46E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:-4.22|P-value:6.25E-5 ID:SGTC_248|Compound:streptozotocin|FD-Score:3.99|P-value:9.24E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:5.01|P-value:1.40E-6 ID:SGTC_346|Compound:4513-0042|FD-Score:-3.49|P-value:7.77E-4 ID:SGTC_529|Compound:4076-0302|FD-Score:3.36|P-value:7.79E-4 ID:SGTC_536|Compound:3852-0578|FD-Score:3.58|P-value:3.92E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:3.40|P-value:6.85E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:-4.54|P-value:1.80E-5 ID:SGTC_672|Compound:1441-0018|FD-Score:4.24|P-value:3.53E-5 ID:SGTC_673|Compound:1082-0474|FD-Score:6.76|P-value:1.55E-10 ID:SGTC_714|Compound:4296-0578|FD-Score:3.36|P-value:7.98E-4 ID:SGTC_744|Compound:1594-0068|FD-Score:5.12|P-value:8.56E-7 ID:SGTC_994|Compound:3910-0338|FD-Score:4.25|P-value:3.43E-5 ID:SGTC_1031|Compound:k035-0009|FD-Score:4.03|P-value:8.00E-5 ID:SGTC_1035|Compound:k081-0041|FD-Score:-4.05|P-value:1.17E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:3.58|P-value:3.89E-4 ID:SGTC_1274|Compound:0865-0114|FD-Score:3.73|P-value:2.34E-4 ID:SGTC_1629|Compound:st005085|FD-Score:-3.56|P-value:6.24E-4 ID:SGTC_1677|Compound:st019204|FD-Score:-3.69|P-value:4.15E-4 ID:SGTC_1725|Compound:st036284|FD-Score:3.50|P-value:4.97E-4 ID:SGTC_1784|Compound:st039334|FD-Score:3.37|P-value:7.71E-4 ID:SGTC_1915|Compound:st067105|FD-Score:3.90|P-value:1.29E-4 ID:SGTC_2000|Compound:st075254|FD-Score:3.36|P-value:7.92E-4 ID:SGTC_2104|Compound:5389443|FD-Score:-3.82|P-value:2.67E-4 ID:SGTC_2106|Compound:5357045|FD-Score:-4.41|P-value:2.97E-5 ID:SGTC_2109|Compound:5483026|FD-Score:3.67|P-value:2.80E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.57|P-value:4.01E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.91|P-value:1.21E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:4.50|P-value:1.25E-5 ID:SGTC_2342|Compound:7990637|FD-Score:-3.47|P-value:8.28E-4 ID:SGTC_2351|Compound:9026858|FD-Score:-5.48|P-value:2.87E-7 ID:SGTC_2412|Compound:st077135|FD-Score:4.63|P-value:7.26E-6 ID:SGTC_2418|Compound:pentamidine|FD-Score:-3.77|P-value:3.10E-4 ID:SGTC_2421|Compound:5690543|FD-Score:4.03|P-value:7.77E-5 ID:SGTC_2431|Compound:5490079|FD-Score:-3.75|P-value:3.34E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:5.51|P-value:1.32E-7 ID:SGTC_2708|Compound:st078559|FD-Score:3.62|P-value:3.37E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:-3.50|P-value:7.57E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.33|P-value:8.80E-4 ID:SGTC_2845|Compound:9003024|FD-Score:4.94|P-value:1.96E-6 ID:SGTC_2881|Compound:9058953|FD-Score:-3.49|P-value:7.74E-4 ID:SGTC_2889|Compound:9059656|FD-Score:3.34|P-value:8.37E-4 ID:SGTC_2946|Compound:9065502|FD-Score:3.82|P-value:1.69E-4 ID:SGTC_2947|Compound:9072639|FD-Score:-3.61|P-value:5.25E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-4.14|P-value:8.35E-5 ID:SGTC_2964|Compound:9083345|FD-Score:4.28|P-value:3.07E-5 ID:SGTC_2972|Compound:9093619|FD-Score:3.95|P-value:1.06E-4 ID:SGTC_3118|Compound:9124250|FD-Score:3.67|P-value:2.83E-4 ID:SGTC_3197|Compound:9113331|FD-Score:-3.48|P-value:8.12E-4 ID:SGTC_3247|Compound:9135149|FD-Score:5.01|P-value:1.43E-6 ID:SGTC_67|Compound:0335-0847|FD-Score:4.16|P-value:4.82E-5 ID:SGTC_70|Compound:0141-0289|FD-Score:3.29|P-value:9.93E-4 ID:SGTC_202|Compound:3594-0513|FD-Score:4.10|P-value:6.07E-5 ID:SGTC_212|Compound:3807-4682|FD-Score:4.18|P-value:4.45E-5 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:-3.47|P-value:8.46E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:-4.22|P-value:6.25E-5 ID:SGTC_248|Compound:streptozotocin|FD-Score:3.99|P-value:9.24E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:5.01|P-value:1.40E-6 ID:SGTC_346|Compound:4513-0042|FD-Score:-3.49|P-value:7.77E-4 ID:SGTC_529|Compound:4076-0302|FD-Score:3.36|P-value:7.79E-4 ID:SGTC_536|Compound:3852-0578|FD-Score:3.58|P-value:3.92E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:3.40|P-value:6.85E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:-4.54|P-value:1.80E-5 ID:SGTC_672|Compound:1441-0018|FD-Score:4.24|P-value:3.53E-5 ID:SGTC_673|Compound:1082-0474|FD-Score:6.76|P-value:1.55E-10 ID:SGTC_714|Compound:4296-0578|FD-Score:3.36|P-value:7.98E-4 ID:SGTC_744|Compound:1594-0068|FD-Score:5.12|P-value:8.56E-7 ID:SGTC_994|Compound:3910-0338|FD-Score:4.25|P-value:3.43E-5 ID:SGTC_1031|Compound:k035-0009|FD-Score:4.03|P-value:8.00E-5 ID:SGTC_1035|Compound:k081-0041|FD-Score:-4.05|P-value:1.17E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:3.58|P-value:3.89E-4 ID:SGTC_1274|Compound:0865-0114|FD-Score:3.73|P-value:2.34E-4 ID:SGTC_1629|Compound:st005085|FD-Score:-3.56|P-value:6.24E-4 ID:SGTC_1677|Compound:st019204|FD-Score:-3.69|P-value:4.15E-4 ID:SGTC_1725|Compound:st036284|FD-Score:3.50|P-value:4.97E-4 ID:SGTC_1784|Compound:st039334|FD-Score:3.37|P-value:7.71E-4 ID:SGTC_1915|Compound:st067105|FD-Score:3.90|P-value:1.29E-4 ID:SGTC_2000|Compound:st075254|FD-Score:3.36|P-value:7.92E-4 ID:SGTC_2104|Compound:5389443|FD-Score:-3.82|P-value:2.67E-4 ID:SGTC_2106|Compound:5357045|FD-Score:-4.41|P-value:2.97E-5 ID:SGTC_2109|Compound:5483026|FD-Score:3.67|P-value:2.80E-4 ID:SGTC_2128|Compound:5255449|FD-Score:3.57|P-value:4.01E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.91|P-value:1.21E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:4.50|P-value:1.25E-5 ID:SGTC_2342|Compound:7990637|FD-Score:-3.47|P-value:8.28E-4 ID:SGTC_2351|Compound:9026858|FD-Score:-5.48|P-value:2.87E-7 ID:SGTC_2412|Compound:st077135|FD-Score:4.63|P-value:7.26E-6 ID:SGTC_2418|Compound:pentamidine|FD-Score:-3.77|P-value:3.10E-4 ID:SGTC_2421|Compound:5690543|FD-Score:4.03|P-value:7.77E-5 ID:SGTC_2431|Compound:5490079|FD-Score:-3.75|P-value:3.34E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:5.51|P-value:1.32E-7 ID:SGTC_2708|Compound:st078559|FD-Score:3.62|P-value:3.37E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:-3.50|P-value:7.57E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.33|P-value:8.80E-4 ID:SGTC_2845|Compound:9003024|FD-Score:4.94|P-value:1.96E-6 ID:SGTC_2881|Compound:9058953|FD-Score:-3.49|P-value:7.74E-4 ID:SGTC_2889|Compound:9059656|FD-Score:3.34|P-value:8.37E-4 ID:SGTC_2946|Compound:9065502|FD-Score:3.82|P-value:1.69E-4 ID:SGTC_2947|Compound:9072639|FD-Score:-3.61|P-value:5.25E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-4.14|P-value:8.35E-5 ID:SGTC_2964|Compound:9083345|FD-Score:4.28|P-value:3.07E-5 ID:SGTC_2972|Compound:9093619|FD-Score:3.95|P-value:1.06E-4 ID:SGTC_3118|Compound:9124250|FD-Score:3.67|P-value:2.83E-4 ID:SGTC_3197|Compound:9113331|FD-Score:-3.48|P-value:8.12E-4 ID:SGTC_3247|Compound:9135149|FD-Score:5.01|P-value:1.43E-6

Top fitness defect scores for YBL039C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_673 1082-0474 6.76 110.0 µM 1.55E-10 RSC complex & mRNA processing 19 7.09E-12
2 SGTC_2544 sphondin 5.51 68.5 µM 1.32E-7 RNA pol III & RNase P/MRP 10 1.75E-8
3 SGTC_744 1594-0068 5.12 283.0 µM 8.56E-7 amide catabolism 8 1.52E-7
4 SGTC_312 4092-0653 5.01 6.8 µM 1.40E-6 ergosterol depletion effects on membrane 57 2.68E-7
5 SGTC_3247 9135149 5.01 49.5 µM 1.43E-6 DNA damage response 16 2.75E-7
6 SGTC_2845 9003024 4.94 43.0 µM 1.96E-6 amide catabolism 25 3.96E-7
7 SGTC_2412 st077135 4.63 33.4 µM 7.26E-6 11 1.79E-6
8 SGTC_2250 alverine citrate 4.50 130.0 µM 1.25E-5 124 3.33E-6
9 SGTC_2964 9083345 4.28 53.4 µM 3.07E-5 16 9.40E-6
10 SGTC_994 3910-0338 4.25 138.0 µM 3.43E-5 calcium & mitochondrial duress 38 1.07E-5
11 SGTC_672 1441-0018 4.24 53.7 µM 3.53E-5 14 1.10E-5
12 SGTC_212 3807-4682 4.18 38.8 µM 4.45E-5 tubulin folding & SWR complex 58 1.44E-5
13 SGTC_67 0335-0847 4.16 266.0 µM 4.82E-5 ubiquinone biosynthesis & proteasome 106 1.58E-5
14 SGTC_202 3594-0513 4.10 34.8 µM 6.07E-5 PDR1 8 2.06E-5
15 SGTC_2421 5690543 4.03 90.7 µM 7.77E-5 17 2.73E-5
16 SGTC_1031 k035-0009 4.03 16.0 µM 8.00E-5 27 2.82E-5
17 SGTC_248 streptozotocin 3.99 5.2 mM 9.24E-5 DNA damage response 26 3.34E-5
18 SGTC_2972 9093619 3.95 59.9 µM 1.06E-4 19 3.92E-5
19 SGTC_2200 6600604 3.91 21.9 µM 1.21E-4 tubulin folding & SWR complex 54 4.52E-5
20 SGTC_1915 st067105 3.90 30.6 µM 1.29E-4 RNA pol III & RNase P/MRP 46 4.90E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.176 1.30E-24 YOR116C RPO31 het RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.168 1.23E-22 YBR154C RPB5 het RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
0.155 1.79E-19 YDR225W HTA1 hom Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
0.135 3.95E-15 YOR191W ULS1 hom Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.135 4.70E-15 YER165W PAB1 het Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
0.118 8.48E-12 YOL006C TOP1 hom Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination
0.116 2.05E-11 YJL081C ARP4 het Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
0.113 5.62E-11 YML031W NDC1 het Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
0.112 9.39E-11 YFL002C SPB4 het Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
0.108 3.17E-10 YNL248C RPA49 hom RNA polymerase I subunit A49
0.106 6.41E-10 YDR540C IRC4 hom Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
0.106 7.96E-10 YNR016C ACC1 het Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
0.097 2.07E-8 YJL148W RPA34 hom RNA polymerase I subunit A34.5
0.096 2.33E-8 YBR202W MCM7 het Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
0.095 3.61E-8 YBL092W RPL32 het Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog