HIPHOP chemogenomics database

YBL039C / URA7

Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication

Zygosity: Homozygous strain

fixedexpanded
Profile for YBL039C / URA7

Click on Significant Values for Screen Details

Top fitness defect scores for YBL039C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_673	1082-0474	6.76	110.0 µM	1.55E-10	RSC complex & mRNA processing	19	7.09E-12
2	SGTC_2544	sphondin	5.51	68.5 µM	1.32E-7	RNA pol III & RNase P/MRP	10	1.75E-8
3	SGTC_744	1594-0068	5.12	283.0 µM	8.56E-7	amide catabolism	8	1.52E-7
4	SGTC_312	4092-0653	5.01	6.8 µM	1.40E-6	ergosterol depletion effects on membrane	57	2.68E-7
5	SGTC_3247	9135149	5.01	49.5 µM	1.43E-6	DNA damage response	16	2.75E-7
6	SGTC_2845	9003024	4.94	43.0 µM	1.96E-6	amide catabolism	25	3.96E-7
7	SGTC_2412	st077135	4.63	33.4 µM	7.26E-6		11	1.79E-6
8	SGTC_2250	alverine citrate	4.50	130.0 µM	1.25E-5		124	3.33E-6
9	SGTC_2964	9083345	4.28	53.4 µM	3.07E-5		16	9.40E-6
10	SGTC_994	3910-0338	4.25	138.0 µM	3.43E-5	calcium & mitochondrial duress	38	1.07E-5
11	SGTC_672	1441-0018	4.24	53.7 µM	3.53E-5		14	1.10E-5
12	SGTC_212	3807-4682	4.18	38.8 µM	4.45E-5	tubulin folding & SWR complex	58	1.44E-5
13	SGTC_67	0335-0847	4.16	266.0 µM	4.82E-5	ubiquinone biosynthesis & proteasome	106	1.58E-5
14	SGTC_202	3594-0513	4.10	34.8 µM	6.07E-5	PDR1	8	2.06E-5
15	SGTC_2421	5690543	4.03	90.7 µM	7.77E-5		17	2.73E-5
16	SGTC_1031	k035-0009	4.03	16.0 µM	8.00E-5		27	2.82E-5
17	SGTC_248	streptozotocin	3.99	5.2 mM	9.24E-5	DNA damage response	26	3.34E-5
18	SGTC_2972	9093619	3.95	59.9 µM	1.06E-4		19	3.92E-5
19	SGTC_2200	6600604	3.91	21.9 µM	1.21E-4	tubulin folding & SWR complex	54	4.52E-5
20	SGTC_1915	st067105	3.90	30.6 µM	1.29E-4	RNA pol III & RNase P/MRP	46	4.90E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.176	1.30E-24	YOR116C	RPO31	het	RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.168	1.23E-22	YBR154C	RPB5	het	RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
0.155	1.79E-19	YDR225W	HTA1	hom	Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
0.135	3.95E-15	YOR191W	ULS1	hom	Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.135	4.70E-15	YER165W	PAB1	het	Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
0.118	8.48E-12	YOL006C	TOP1	hom	Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination
0.116	2.05E-11	YJL081C	ARP4	het	Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
0.113	5.62E-11	YML031W	NDC1	het	Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
0.112	9.39E-11	YFL002C	SPB4	het	Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
0.108	3.17E-10	YNL248C	RPA49	hom	RNA polymerase I subunit A49
0.106	6.41E-10	YDR540C	IRC4	hom	Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
0.106	7.96E-10	YNR016C	ACC1	het	Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
0.097	2.07E-8	YJL148W	RPA34	hom	RNA polymerase I subunit A34.5
0.096	2.33E-8	YBR202W	MCM7	het	Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
0.095	3.61E-8	YBL092W	RPL32	het	Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog