YBL061C / SKT5

Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YBL061C / SKT5

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:7.50|P-value:4.63E-13 ID:SGTC_95|Compound:0471-0001|FD-Score:5.75|P-value:2.16E-8 ID:SGTC_138|Compound:0850-0259|FD-Score:6.59|P-value:1.69E-10 ID:SGTC_151|Compound:3013-0144|FD-Score:4.49|P-value:9.35E-6 ID:SGTC_181|Compound:1447-1644|FD-Score:3.94|P-value:8.71E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:5.52|P-value:7.38E-8 ID:SGTC_354|Compound:2783-3500|FD-Score:6.65|P-value:1.23E-10 ID:SGTC_360|Compound:0957-0399|FD-Score:5.97|P-value:6.44E-9 ID:SGTC_514|Compound:4466-0038|FD-Score:4.31|P-value:2.01E-5 ID:SGTC_576|Compound:1073-0113|FD-Score:3.32|P-value:7.94E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:5.98|P-value:6.17E-9 ID:SGTC_593|Compound:1203-0218|FD-Score:3.88|P-value:1.10E-4 ID:SGTC_595|Compound:3909-8003|FD-Score:3.70|P-value:2.13E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:-3.81|P-value:1.39E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:3.82|P-value:1.36E-4 ID:SGTC_731|Compound:3966-0296|FD-Score:3.91|P-value:9.79E-5 ID:SGTC_790|Compound:1575-0012|FD-Score:3.35|P-value:7.14E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:7.24|P-value:2.67E-12 ID:SGTC_883|Compound:0850-0245|FD-Score:3.48|P-value:4.56E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:5.62|P-value:4.42E-8 ID:SGTC_916|Compound:4466-0038|FD-Score:16.00|P-value:4.66E-53 ID:SGTC_921|Compound:1561-0023|FD-Score:3.50|P-value:4.36E-4 ID:SGTC_928|Compound:2764-0428|FD-Score:4.71|P-value:3.58E-6 ID:SGTC_964|Compound:1287-0107|FD-Score:6.26|P-value:1.24E-9 ID:SGTC_997|Compound:3996-0078|FD-Score:3.47|P-value:4.79E-4 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.60|P-value:3.03E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:3.47|P-value:4.76E-4 ID:SGTC_1105|Compound:epirubicin|FD-Score:-3.47|P-value:4.66E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.85|P-value:1.24E-4 ID:SGTC_1221|Compound:2958-7315|FD-Score:5.84|P-value:1.31E-8 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.28|P-value:9.00E-4 ID:SGTC_1431|Compound:4049-0260|FD-Score:3.56|P-value:3.47E-4 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.51|P-value:4.20E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:3.49|P-value:4.49E-4 ID:SGTC_1594|Compound:piperine|FD-Score:3.33|P-value:7.56E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.48|P-value:4.68E-4 ID:SGTC_1664|Compound:st014217|FD-Score:3.39|P-value:6.30E-4 ID:SGTC_1687|Compound:st020778|FD-Score:4.04|P-value:5.99E-5 ID:SGTC_1754|Compound:st042065|FD-Score:11.10|P-value:1.22E-26 ID:SGTC_1758|Compound:st042942|FD-Score:3.92|P-value:9.30E-5 ID:SGTC_1760|Compound:st045453|FD-Score:8.90|P-value:1.20E-17 ID:SGTC_1792|Compound:st045773|FD-Score:3.84|P-value:1.26E-4 ID:SGTC_1803|Compound:st050068|FD-Score:3.76|P-value:1.70E-4 ID:SGTC_2149|Compound:5554786|FD-Score:3.28|P-value:8.85E-4 ID:SGTC_2286|Compound:7945697|FD-Score:3.48|P-value:4.61E-4 ID:SGTC_2289|Compound:7946404|FD-Score:5.13|P-value:5.25E-7 ID:SGTC_2321|Compound:7390090|FD-Score:3.47|P-value:4.69E-4 ID:SGTC_2332|Compound:7982454|FD-Score:3.93|P-value:9.16E-5 ID:SGTC_2400|Compound:5203494|FD-Score:4.36|P-value:1.67E-5 ID:SGTC_2467|Compound:5474540|FD-Score:4.06|P-value:5.44E-5 ID:SGTC_2475|Compound:5812728|FD-Score:4.77|P-value:2.79E-6 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:3.31|P-value:8.14E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:5.36|P-value:1.67E-7 ID:SGTC_2584|Compound:androsterone|FD-Score:3.62|P-value:2.80E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:7.19|P-value:3.85E-12 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:3.38|P-value:6.35E-4 ID:SGTC_2781|Compound:5567276|FD-Score:3.68|P-value:2.27E-4 ID:SGTC_2837|Compound:9008476|FD-Score:5.19|P-value:3.94E-7 ID:SGTC_2842|Compound:9000675|FD-Score:3.59|P-value:3.15E-4 ID:SGTC_2844|Compound:9010103|FD-Score:4.36|P-value:1.63E-5 ID:SGTC_2912|Compound:7964298|FD-Score:12.40|P-value:2.42E-32 ID:SGTC_2917|Compound:7979103|FD-Score:3.73|P-value:1.92E-4 ID:SGTC_2950|Compound:9072895|FD-Score:5.01|P-value:9.12E-7 ID:SGTC_2958|Compound:9077254|FD-Score:6.37|P-value:6.38E-10 ID:SGTC_2987|Compound:9018338|FD-Score:5.53|P-value:7.09E-8 ID:SGTC_2992|Compound:9074591|FD-Score:3.65|P-value:2.51E-4 ID:SGTC_3045|Compound:9092950|FD-Score:-3.54|P-value:3.66E-4 ID:SGTC_3065|Compound:9113888|FD-Score:4.25|P-value:2.56E-5 ID:SGTC_3074|Compound:9116018|FD-Score:3.36|P-value:6.82E-4 ID:SGTC_3201|Compound:9113999|FD-Score:3.66|P-value:2.47E-4 ID:SGTC_3266|Compound:9136860|FD-Score:4.97|P-value:1.09E-6 ID:SGTC_3311|Compound:9131146|FD-Score:4.42|P-value:1.30E-5 ID:SGTC_3321|Compound:9137793|FD-Score:6.88|P-value:2.87E-11 ID:SGTC_3350|Compound:9150499|FD-Score:4.14|P-value:4.08E-5 ID:SGTC_3351|Compound:9150312|FD-Score:3.47|P-value:4.81E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:7.50|P-value:4.63E-13 ID:SGTC_95|Compound:0471-0001|FD-Score:5.75|P-value:2.16E-8 ID:SGTC_138|Compound:0850-0259|FD-Score:6.59|P-value:1.69E-10 ID:SGTC_151|Compound:3013-0144|FD-Score:4.49|P-value:9.35E-6 ID:SGTC_181|Compound:1447-1644|FD-Score:3.94|P-value:8.71E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:5.52|P-value:7.38E-8 ID:SGTC_354|Compound:2783-3500|FD-Score:6.65|P-value:1.23E-10 ID:SGTC_360|Compound:0957-0399|FD-Score:5.97|P-value:6.44E-9 ID:SGTC_514|Compound:4466-0038|FD-Score:4.31|P-value:2.01E-5 ID:SGTC_576|Compound:1073-0113|FD-Score:3.32|P-value:7.94E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:5.98|P-value:6.17E-9 ID:SGTC_593|Compound:1203-0218|FD-Score:3.88|P-value:1.10E-4 ID:SGTC_595|Compound:3909-8003|FD-Score:3.70|P-value:2.13E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:-3.81|P-value:1.39E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:3.82|P-value:1.36E-4 ID:SGTC_731|Compound:3966-0296|FD-Score:3.91|P-value:9.79E-5 ID:SGTC_790|Compound:1575-0012|FD-Score:3.35|P-value:7.14E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:7.24|P-value:2.67E-12 ID:SGTC_883|Compound:0850-0245|FD-Score:3.48|P-value:4.56E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:5.62|P-value:4.42E-8 ID:SGTC_916|Compound:4466-0038|FD-Score:16.00|P-value:4.66E-53 ID:SGTC_921|Compound:1561-0023|FD-Score:3.50|P-value:4.36E-4 ID:SGTC_928|Compound:2764-0428|FD-Score:4.71|P-value:3.58E-6 ID:SGTC_964|Compound:1287-0107|FD-Score:6.26|P-value:1.24E-9 ID:SGTC_997|Compound:3996-0078|FD-Score:3.47|P-value:4.79E-4 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.60|P-value:3.03E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:3.47|P-value:4.76E-4 ID:SGTC_1105|Compound:epirubicin|FD-Score:-3.47|P-value:4.66E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.85|P-value:1.24E-4 ID:SGTC_1221|Compound:2958-7315|FD-Score:5.84|P-value:1.31E-8 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.28|P-value:9.00E-4 ID:SGTC_1431|Compound:4049-0260|FD-Score:3.56|P-value:3.47E-4 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.51|P-value:4.20E-4 ID:SGTC_1589|Compound:2',5'-dimethoxyflavone|FD-Score:3.49|P-value:4.49E-4 ID:SGTC_1594|Compound:piperine|FD-Score:3.33|P-value:7.56E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.48|P-value:4.68E-4 ID:SGTC_1664|Compound:st014217|FD-Score:3.39|P-value:6.30E-4 ID:SGTC_1687|Compound:st020778|FD-Score:4.04|P-value:5.99E-5 ID:SGTC_1754|Compound:st042065|FD-Score:11.10|P-value:1.22E-26 ID:SGTC_1758|Compound:st042942|FD-Score:3.92|P-value:9.30E-5 ID:SGTC_1760|Compound:st045453|FD-Score:8.90|P-value:1.20E-17 ID:SGTC_1792|Compound:st045773|FD-Score:3.84|P-value:1.26E-4 ID:SGTC_1803|Compound:st050068|FD-Score:3.76|P-value:1.70E-4 ID:SGTC_2149|Compound:5554786|FD-Score:3.28|P-value:8.85E-4 ID:SGTC_2286|Compound:7945697|FD-Score:3.48|P-value:4.61E-4 ID:SGTC_2289|Compound:7946404|FD-Score:5.13|P-value:5.25E-7 ID:SGTC_2321|Compound:7390090|FD-Score:3.47|P-value:4.69E-4 ID:SGTC_2332|Compound:7982454|FD-Score:3.93|P-value:9.16E-5 ID:SGTC_2400|Compound:5203494|FD-Score:4.36|P-value:1.67E-5 ID:SGTC_2467|Compound:5474540|FD-Score:4.06|P-value:5.44E-5 ID:SGTC_2475|Compound:5812728|FD-Score:4.77|P-value:2.79E-6 ID:SGTC_2529|Compound:gangaleoidin|FD-Score:3.31|P-value:8.14E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:5.36|P-value:1.67E-7 ID:SGTC_2584|Compound:androsterone|FD-Score:3.62|P-value:2.80E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:7.19|P-value:3.85E-12 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:3.38|P-value:6.35E-4 ID:SGTC_2781|Compound:5567276|FD-Score:3.68|P-value:2.27E-4 ID:SGTC_2837|Compound:9008476|FD-Score:5.19|P-value:3.94E-7 ID:SGTC_2842|Compound:9000675|FD-Score:3.59|P-value:3.15E-4 ID:SGTC_2844|Compound:9010103|FD-Score:4.36|P-value:1.63E-5 ID:SGTC_2912|Compound:7964298|FD-Score:12.40|P-value:2.42E-32 ID:SGTC_2917|Compound:7979103|FD-Score:3.73|P-value:1.92E-4 ID:SGTC_2950|Compound:9072895|FD-Score:5.01|P-value:9.12E-7 ID:SGTC_2958|Compound:9077254|FD-Score:6.37|P-value:6.38E-10 ID:SGTC_2987|Compound:9018338|FD-Score:5.53|P-value:7.09E-8 ID:SGTC_2992|Compound:9074591|FD-Score:3.65|P-value:2.51E-4 ID:SGTC_3045|Compound:9092950|FD-Score:-3.54|P-value:3.66E-4 ID:SGTC_3065|Compound:9113888|FD-Score:4.25|P-value:2.56E-5 ID:SGTC_3074|Compound:9116018|FD-Score:3.36|P-value:6.82E-4 ID:SGTC_3201|Compound:9113999|FD-Score:3.66|P-value:2.47E-4 ID:SGTC_3266|Compound:9136860|FD-Score:4.97|P-value:1.09E-6 ID:SGTC_3311|Compound:9131146|FD-Score:4.42|P-value:1.30E-5 ID:SGTC_3321|Compound:9137793|FD-Score:6.88|P-value:2.87E-11 ID:SGTC_3350|Compound:9150499|FD-Score:4.14|P-value:4.08E-5 ID:SGTC_3351|Compound:9150312|FD-Score:3.47|P-value:4.81E-4

Top fitness defect scores for YBL061C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_916 4466-0038 16.00 42.0 nM 4.66E-53 cell wall 2 2.91E-58
2 SGTC_2912 7964298 12.40 10.9 µM 2.42E-32 cell wall 9 1.91E-35
3 SGTC_1754 st042065 11.10 16.1 µM 1.22E-26 NEO1-PIK1 16 3.65E-29
4 SGTC_1760 st045453 8.90 10.4 µM 1.20E-17 NEO1-PIK1 42 2.92E-19
5 SGTC_58 0957-0393 7.50 9.0 µM 4.63E-13 cell wall 143 3.24E-14
6 SGTC_863 0466-0191 7.24 129.0 µM 2.67E-12 cell wall 22 2.23E-13
7 SGTC_2619 obliquin 7.19 87.9 µM 3.85E-12 cell wall 55 3.33E-13
8 SGTC_3321 9137793 6.88 26.8 µM 2.87E-11 cell wall 25 3.04E-12
9 SGTC_354 2783-3500 6.65 4.9 µM 1.23E-10 cell wall 15 1.50E-11
10 SGTC_138 0850-0259 6.59 40.9 µM 1.69E-10 cell wall 87 2.14E-11
11 SGTC_2958 9077254 6.37 16.7 µM 6.38E-10 3 9.21E-11
12 SGTC_964 1287-0107 6.26 19.4 µM 1.24E-9 cell wall 11 1.91E-10
13 SGTC_586 0957-0265 5.98 8.2 µM 6.17E-9 cell wall 18 1.12E-9
14 SGTC_360 0957-0399 5.97 1.0 µM 6.44E-9 cell wall 13 1.17E-9
15 SGTC_1221 2958-7315 5.84 257.0 µM 1.31E-8 2 2.56E-9
16 SGTC_95 0471-0001 5.75 561.1 µM 2.16E-8 5 4.42E-9
17 SGTC_907 3448-6986 5.62 75.5 µM 4.42E-8 3 9.73E-9
18 SGTC_2987 9018338 5.53 71.4 µM 7.09E-8 RPP1 & pyrimidine depletion 4 1.64E-8
19 SGTC_351 caspofungin 5.52 25.0 nM 7.38E-8 cell wall 45 1.71E-8
20 SGTC_2577 2',4'-dihydroxy-4-methoxychalcone 5.36 15.3 µM 1.67E-7 12 4.18E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.464 2.62E-178 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.456 9.74E-172 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.423 1.43E-145 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.403 6.41E-131 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.394 5.08E-125 YPL066W RGL1 hom Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
0.371 6.35E-110 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.370 3.98E-109 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.347 1.61E-95 YDR348C PAL1 hom Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.334 5.89E-88 YBL006C LDB7 hom Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
0.325 1.62E-83 YPR165W RHO1 het GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
0.320 7.08E-81 YDL066W IDP1 hom Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
0.318 1.03E-79 YLR330W CHS5 hom Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane
0.308 1.60E-74 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.306 2.08E-73 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.296 8.33E-69 YBR091C TIM12 het Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins