YBL068W / PRS4

5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YBL068W / PRS4

Click on Significant Values for Screen Details ID:SGTC_127|Compound:0180-0333|FD-Score:3.01|P-value:2.44E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:4.52|P-value:9.20E-8 ID:SGTC_333|Compound:3486-0131|FD-Score:3.31|P-value:6.38E-5 ID:SGTC_342|Compound:k048-0088|FD-Score:-2.99|P-value:3.61E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:-2.85|P-value:6.33E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:3.32|P-value:6.11E-5 ID:SGTC_681|Compound:1642-0006|FD-Score:-3.23|P-value:1.26E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:4.34|P-value:2.64E-7 ID:SGTC_705|Compound:1124-0212|FD-Score:6.55|P-value:2.37E-14 ID:SGTC_710|Compound:3448-4187|FD-Score:3.68|P-value:1.04E-5 ID:SGTC_758|Compound:0868-0029|FD-Score:-4.86|P-value:1.61E-8 ID:SGTC_770|Compound:0389-1121|FD-Score:3.24|P-value:8.77E-5 ID:SGTC_791|Compound:1348-1292|FD-Score:2.83|P-value:5.23E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:3.57|P-value:1.82E-5 ID:SGTC_850|Compound:0388-0143|FD-Score:-3.01|P-value:3.26E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:3.61|P-value:1.46E-5 ID:SGTC_1255|Compound:0566-0045|FD-Score:2.80|P-value:5.90E-4 ID:SGTC_1275|Compound:0867-0008|FD-Score:2.73|P-value:7.67E-4 ID:SGTC_1299|Compound:1079-1439|FD-Score:2.75|P-value:7.22E-4 ID:SGTC_1367|Compound:1812-0510|FD-Score:-3.21|P-value:1.37E-4 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:2.88|P-value:4.13E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.01|P-value:2.46E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.34|P-value:5.61E-5 ID:SGTC_1676|Compound:st018489|FD-Score:2.91|P-value:3.75E-4 ID:SGTC_1682|Compound:st012968|FD-Score:-2.90|P-value:5.16E-4 ID:SGTC_1709|Compound:st033179|FD-Score:3.21|P-value:1.00E-4 ID:SGTC_1973|Compound:st070275|FD-Score:2.93|P-value:3.39E-4 ID:SGTC_2062|Compound:5245942|FD-Score:2.68|P-value:9.34E-4 ID:SGTC_2132|Compound:5317594|FD-Score:-4.23|P-value:7.74E-7 ID:SGTC_2133|Compound:5320837|FD-Score:4.81|P-value:1.45E-8 ID:SGTC_2172|Compound:5754775|FD-Score:2.78|P-value:6.33E-4 ID:SGTC_2327|Compound:6189656|FD-Score:3.72|P-value:8.53E-6 ID:SGTC_2395|Compound:5646831|FD-Score:2.91|P-value:3.67E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:2.73|P-value:7.68E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:2.96|P-value:2.96E-4 ID:SGTC_2787|Compound:5284540|FD-Score:3.16|P-value:1.24E-4 ID:SGTC_2820|Compound:7989630|FD-Score:3.05|P-value:2.08E-4 ID:SGTC_2966|Compound:9084041|FD-Score:-3.11|P-value:2.16E-4 ID:SGTC_2967|Compound:9089538|FD-Score:5.53|P-value:9.51E-11 ID:SGTC_2971|Compound:9092186|FD-Score:2.99|P-value:2.59E-4 ID:SGTC_2972|Compound:9093619|FD-Score:-4.85|P-value:1.77E-8 ID:SGTC_3084|Compound:9116877|FD-Score:2.70|P-value:8.63E-4 ID:SGTC_3087|Compound:9117406|FD-Score:2.81|P-value:5.61E-4 ID:SGTC_3125|Compound:9127534|FD-Score:2.68|P-value:9.20E-4 ID:SGTC_3127|Compound:9126946|FD-Score:2.67|P-value:9.63E-4 ID:SGTC_3202|Compound:9113761|FD-Score:7.70|P-value:3.88E-19 ID:SGTC_3212|Compound:9128307|FD-Score:3.70|P-value:9.27E-6 ID:SGTC_3277|Compound:9138724|FD-Score:-2.81|P-value:7.44E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:3.01|P-value:2.44E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:4.52|P-value:9.20E-8 ID:SGTC_333|Compound:3486-0131|FD-Score:3.31|P-value:6.38E-5 ID:SGTC_342|Compound:k048-0088|FD-Score:-2.99|P-value:3.61E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:-2.85|P-value:6.33E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:3.32|P-value:6.11E-5 ID:SGTC_681|Compound:1642-0006|FD-Score:-3.23|P-value:1.26E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:4.34|P-value:2.64E-7 ID:SGTC_705|Compound:1124-0212|FD-Score:6.55|P-value:2.37E-14 ID:SGTC_710|Compound:3448-4187|FD-Score:3.68|P-value:1.04E-5 ID:SGTC_758|Compound:0868-0029|FD-Score:-4.86|P-value:1.61E-8 ID:SGTC_770|Compound:0389-1121|FD-Score:3.24|P-value:8.77E-5 ID:SGTC_791|Compound:1348-1292|FD-Score:2.83|P-value:5.23E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:3.57|P-value:1.82E-5 ID:SGTC_850|Compound:0388-0143|FD-Score:-3.01|P-value:3.26E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:3.61|P-value:1.46E-5 ID:SGTC_1255|Compound:0566-0045|FD-Score:2.80|P-value:5.90E-4 ID:SGTC_1275|Compound:0867-0008|FD-Score:2.73|P-value:7.67E-4 ID:SGTC_1299|Compound:1079-1439|FD-Score:2.75|P-value:7.22E-4 ID:SGTC_1367|Compound:1812-0510|FD-Score:-3.21|P-value:1.37E-4 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:2.88|P-value:4.13E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.01|P-value:2.46E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.34|P-value:5.61E-5 ID:SGTC_1676|Compound:st018489|FD-Score:2.91|P-value:3.75E-4 ID:SGTC_1682|Compound:st012968|FD-Score:-2.90|P-value:5.16E-4 ID:SGTC_1709|Compound:st033179|FD-Score:3.21|P-value:1.00E-4 ID:SGTC_1973|Compound:st070275|FD-Score:2.93|P-value:3.39E-4 ID:SGTC_2062|Compound:5245942|FD-Score:2.68|P-value:9.34E-4 ID:SGTC_2132|Compound:5317594|FD-Score:-4.23|P-value:7.74E-7 ID:SGTC_2133|Compound:5320837|FD-Score:4.81|P-value:1.45E-8 ID:SGTC_2172|Compound:5754775|FD-Score:2.78|P-value:6.33E-4 ID:SGTC_2327|Compound:6189656|FD-Score:3.72|P-value:8.53E-6 ID:SGTC_2395|Compound:5646831|FD-Score:2.91|P-value:3.67E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:2.73|P-value:7.68E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:2.96|P-value:2.96E-4 ID:SGTC_2787|Compound:5284540|FD-Score:3.16|P-value:1.24E-4 ID:SGTC_2820|Compound:7989630|FD-Score:3.05|P-value:2.08E-4 ID:SGTC_2966|Compound:9084041|FD-Score:-3.11|P-value:2.16E-4 ID:SGTC_2967|Compound:9089538|FD-Score:5.53|P-value:9.51E-11 ID:SGTC_2971|Compound:9092186|FD-Score:2.99|P-value:2.59E-4 ID:SGTC_2972|Compound:9093619|FD-Score:-4.85|P-value:1.77E-8 ID:SGTC_3084|Compound:9116877|FD-Score:2.70|P-value:8.63E-4 ID:SGTC_3087|Compound:9117406|FD-Score:2.81|P-value:5.61E-4 ID:SGTC_3125|Compound:9127534|FD-Score:2.68|P-value:9.20E-4 ID:SGTC_3127|Compound:9126946|FD-Score:2.67|P-value:9.63E-4 ID:SGTC_3202|Compound:9113761|FD-Score:7.70|P-value:3.88E-19 ID:SGTC_3212|Compound:9128307|FD-Score:3.70|P-value:9.27E-6 ID:SGTC_3277|Compound:9138724|FD-Score:-2.81|P-value:7.44E-4

Top fitness defect scores for YBL068W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3202 9113761 7.70 49.5 µM 3.88E-19 3 6.63E-15
2 SGTC_705 1124-0212 6.55 310.0 µM 2.37E-14 1 2.94E-11
3 SGTC_2967 9089538 5.53 49.9 µM 9.51E-11 7 1.64E-8
4 SGTC_2133 5320837 4.81 200.0 µM 1.45E-8 ERG2 13 7.62E-7
5 SGTC_314 1671-0008 4.52 2.7 µM 9.20E-8 33 3.13E-6
6 SGTC_700 3453-2283 4.34 142.0 µM 2.64E-7 amide catabolism 18 7.00E-6
7 SGTC_2327 6189656 3.72 200.0 µM 8.53E-6 26 1.00E-4
8 SGTC_3212 9128307 3.70 49.5 µM 9.27E-6 55 1.07E-4
9 SGTC_710 3448-4187 3.68 58.0 µM 1.04E-5 fatty acid desaturase (OLE1) 69 1.16E-4
10 SGTC_978 1416-0512 3.61 87.4 µM 1.46E-5 redox potentiating 36 1.51E-4
11 SGTC_813 k007-0158 3.57 173.0 µM 1.82E-5 fatty acid desaturase (OLE1) 61 1.79E-4
12 SGTC_1568 digitonin 3.34 820.0 nM 5.61E-5 sphingolipid biosynthesis & PDR1 53 4.23E-4
13 SGTC_580 1123-0028 3.32 41.0 µM 6.11E-5 DNA damage response 62 4.52E-4
14 SGTC_333 3486-0131 3.31 40.1 µM 6.38E-5 amide catabolism 24 4.68E-4
15 SGTC_770 0389-1121 3.24 67.2 µM 8.77E-5 RPP1 & pyrimidine depletion 59 5.97E-4
16 SGTC_1709 st033179 3.21 39.1 µM 1.00E-4 40 6.61E-4
17 SGTC_2787 5284540 3.16 71.4 µM 1.24E-4 58 7.79E-4
18 SGTC_2820 7989630 3.05 71.4 µM 2.08E-4 ERG2 52 0.00116
19 SGTC_127 0180-0333 3.01 59.1 µM 2.44E-4 mitochondrial response to ROS 108 0.00131
20 SGTC_1510 1123-0200 3.01 71.5 µM 2.46E-4 97 0.00132

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.203 1.58E-32 YOR291W YPK9 hom Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
0.176 7.27E-25 YML025C YML6 het Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
0.172 1.02E-23 YBR138C_p YBR138C_p hom Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene
0.146 2.28E-17 YGL114W_p YGL114W_p hom Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters
0.145 2.99E-17 YKL100C_p YKL100C_p hom Putative protein of unknown function with similarity to a human minor histocompatibility antigen and signal peptide peptidases; YKL100C is not an essential gene
0.142 1.67E-16 YKL151C YKL151C hom NADHX dehydratase; converts (S)-NADHX to NADH in an ATP-dependent manner; homologous to Carkd in mammals, and the C-terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
0.140 4.13E-16 YML029W USA1 hom Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p
0.137 1.47E-15 YHR118C ORC6 het Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p
0.132 1.39E-14 YGR251W NOP19 het Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes
0.131 2.47E-14 YPR185W ATG13 hom Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; involved in Atg9p, Atg23p, and Atg27p cycling
0.129 6.51E-14 YBR144C_d YBR144C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR144C is not an essential gene
0.123 7.68E-13 YIL132C CSM2 hom Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis
0.120 2.82E-12 YHL045W_d YHL045W_d hom Putative protein of unknown function; not an essential gene
0.115 2.14E-11 YPL246C RBD2 hom Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p
0.114 3.19E-11 YPL263C KEL3 hom Cytoplasmic protein of unknown function