YBL076C / ILS1

Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A

Zygosity: Heterozygous strain
fixedexpanded
Profile for YBL076C / ILS1

Click on Significant Values for Screen Details ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:-3.37|P-value:3.26E-4 ID:SGTC_264|Compound:promethazine|FD-Score:3.16|P-value:6.87E-4 ID:SGTC_296|Compound:1222-0039|FD-Score:3.20|P-value:6.06E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.90|P-value:3.97E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.40|P-value:2.92E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:-3.14|P-value:7.44E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:3.39|P-value:2.99E-4 ID:SGTC_1182|Compound:1222-0449|FD-Score:-3.99|P-value:2.64E-5 ID:SGTC_1346|Compound:1488-2224|FD-Score:3.29|P-value:4.35E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:-3.20|P-value:6.05E-4 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.73|P-value:7.83E-5 ID:SGTC_1444|Compound:4112-3581|FD-Score:-3.25|P-value:4.92E-4 ID:SGTC_1729|Compound:st035615|FD-Score:3.50|P-value:1.94E-4 ID:SGTC_1740|Compound:st038286|FD-Score:-3.78|P-value:6.51E-5 ID:SGTC_1757|Compound:st041620|FD-Score:3.54|P-value:1.67E-4 ID:SGTC_1758|Compound:st042942|FD-Score:3.75|P-value:7.36E-5 ID:SGTC_1823|Compound:3-hydroxy-2-naphthoic acid|FD-Score:4.40|P-value:4.13E-6 ID:SGTC_1845|Compound:st056753|FD-Score:-4.38|P-value:4.61E-6 ID:SGTC_1849|Compound:st056239|FD-Score:3.53|P-value:1.78E-4 ID:SGTC_1853|Compound:st056710|FD-Score:3.35|P-value:3.50E-4 ID:SGTC_1857|Compound:st056007|FD-Score:-5.05|P-value:1.62E-7 ID:SGTC_1961|Compound:st076550|FD-Score:-4.31|P-value:6.46E-6 ID:SGTC_1966|Compound:st070145|FD-Score:-3.86|P-value:4.60E-5 ID:SGTC_1981|Compound:st071826|FD-Score:3.19|P-value:6.27E-4 ID:SGTC_1998|Compound:st075797|FD-Score:3.25|P-value:4.91E-4 ID:SGTC_2162|Compound:5663267|FD-Score:-3.61|P-value:1.28E-4 ID:SGTC_2267|Compound:7931562|FD-Score:-3.15|P-value:7.23E-4 ID:SGTC_2452|Compound:5863136|FD-Score:3.08|P-value:8.97E-4 ID:SGTC_2477|Compound:5967686|FD-Score:4.21|P-value:1.01E-5 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-3.51|P-value:1.86E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:4.68|P-value:1.06E-6 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.19|P-value:6.10E-4 ID:SGTC_2833|Compound:9001315|FD-Score:-3.47|P-value:2.23E-4 ID:SGTC_2936|Compound:9038235|FD-Score:3.59|P-value:1.38E-4 ID:SGTC_2953|Compound:9074162|FD-Score:3.08|P-value:9.00E-4 ID:SGTC_3097|Compound:9117246|FD-Score:3.21|P-value:5.71E-4 ID:SGTC_3237|Compound:9133362|FD-Score:4.83|P-value:4.94E-7 ID:SGTC_3239|Compound:9133757|FD-Score:-3.21|P-value:5.81E-4 ID:SGTC_3315|Compound:9136395|FD-Score:4.45|P-value:3.31E-6 ID:SGTC_3327|Compound:9140648|FD-Score:3.91|P-value:3.76E-5 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:-3.37|P-value:3.26E-4 ID:SGTC_264|Compound:promethazine|FD-Score:3.16|P-value:6.87E-4 ID:SGTC_296|Compound:1222-0039|FD-Score:3.20|P-value:6.06E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-3.90|P-value:3.97E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.40|P-value:2.92E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:-3.14|P-value:7.44E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:3.39|P-value:2.99E-4 ID:SGTC_1182|Compound:1222-0449|FD-Score:-3.99|P-value:2.64E-5 ID:SGTC_1346|Compound:1488-2224|FD-Score:3.29|P-value:4.35E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:-3.20|P-value:6.05E-4 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.73|P-value:7.83E-5 ID:SGTC_1444|Compound:4112-3581|FD-Score:-3.25|P-value:4.92E-4 ID:SGTC_1729|Compound:st035615|FD-Score:3.50|P-value:1.94E-4 ID:SGTC_1740|Compound:st038286|FD-Score:-3.78|P-value:6.51E-5 ID:SGTC_1757|Compound:st041620|FD-Score:3.54|P-value:1.67E-4 ID:SGTC_1758|Compound:st042942|FD-Score:3.75|P-value:7.36E-5 ID:SGTC_1823|Compound:3-hydroxy-2-naphthoic acid|FD-Score:4.40|P-value:4.13E-6 ID:SGTC_1845|Compound:st056753|FD-Score:-4.38|P-value:4.61E-6 ID:SGTC_1849|Compound:st056239|FD-Score:3.53|P-value:1.78E-4 ID:SGTC_1853|Compound:st056710|FD-Score:3.35|P-value:3.50E-4 ID:SGTC_1857|Compound:st056007|FD-Score:-5.05|P-value:1.62E-7 ID:SGTC_1961|Compound:st076550|FD-Score:-4.31|P-value:6.46E-6 ID:SGTC_1966|Compound:st070145|FD-Score:-3.86|P-value:4.60E-5 ID:SGTC_1981|Compound:st071826|FD-Score:3.19|P-value:6.27E-4 ID:SGTC_1998|Compound:st075797|FD-Score:3.25|P-value:4.91E-4 ID:SGTC_2162|Compound:5663267|FD-Score:-3.61|P-value:1.28E-4 ID:SGTC_2267|Compound:7931562|FD-Score:-3.15|P-value:7.23E-4 ID:SGTC_2452|Compound:5863136|FD-Score:3.08|P-value:8.97E-4 ID:SGTC_2477|Compound:5967686|FD-Score:4.21|P-value:1.01E-5 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-3.51|P-value:1.86E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:4.68|P-value:1.06E-6 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.19|P-value:6.10E-4 ID:SGTC_2833|Compound:9001315|FD-Score:-3.47|P-value:2.23E-4 ID:SGTC_2936|Compound:9038235|FD-Score:3.59|P-value:1.38E-4 ID:SGTC_2953|Compound:9074162|FD-Score:3.08|P-value:9.00E-4 ID:SGTC_3097|Compound:9117246|FD-Score:3.21|P-value:5.71E-4 ID:SGTC_3237|Compound:9133362|FD-Score:4.83|P-value:4.94E-7 ID:SGTC_3239|Compound:9133757|FD-Score:-3.21|P-value:5.81E-4 ID:SGTC_3315|Compound:9136395|FD-Score:4.45|P-value:3.31E-6 ID:SGTC_3327|Compound:9140648|FD-Score:3.91|P-value:3.76E-5

Top fitness defect scores for YBL076C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3237 9133362 4.83 49.5 µM 4.94E-7 3 6.73E-7 1.08
2 SGTC_2753 fluvastatin 4.68 17.1 µM 1.06E-6 azole & statin 6 1.42E-6 0.49
3 SGTC_3315 9136395 4.45 68.5 µM 3.31E-6 5 4.31E-6 0.34
4 SGTC_1823 3-hydroxy-2-naphthoic acid 4.40 106.0 µM 4.13E-6 2 5.35E-6 0.51
5 SGTC_2477 5967686 4.21 25.2 µM 1.01E-5 6 1.28E-5 0.25
6 SGTC_3327 9140648 3.91 26.9 µM 3.76E-5 13 4.62E-5 0.03
7 SGTC_1758 st042942 3.75 75.2 µM 7.36E-5 4 8.90E-5 0.16
8 SGTC_1424 4048-1941 3.73 275.0 µM 7.83E-5 4 9.46E-5 0.01
9 SGTC_2936 9038235 3.59 15.6 µM 1.38E-4 11 1.64E-4 0.11
10 SGTC_1757 st041620 3.54 31.8 µM 1.67E-4 2 1.98E-4 0.32
11 SGTC_1849 st056239 3.53 19.2 µM 1.78E-4 RPP1 & pyrimidine depletion 5 2.11E-4 0.09
12 SGTC_1729 st035615 3.50 46.0 µM 1.94E-4 10 2.29E-4 0.11
13 SGTC_963 1269-2853 3.39 151.0 µM 2.99E-4 7 3.50E-4 0.13
14 SGTC_1853 st056710 3.35 91.2 µM 3.50E-4 amide catabolism 10 4.08E-4 0.02
15 SGTC_1346 1488-2224 3.29 62.4 µM 4.35E-4 20 5.04E-4 0.04
16 SGTC_1998 st075797 3.25 76.8 µM 4.91E-4 mitochondrial response to ROS 6 5.68E-4 0.14
17 SGTC_3097 9117246 3.21 49.5 µM 5.71E-4 6 6.58E-4 0.08
18 SGTC_296 1222-0039 3.20 74.9 µM 6.06E-4 5 6.98E-4 0.07
19 SGTC_2765 myclobutanil 3.19 7.7 µM 6.10E-4 azole & statin 22 7.03E-4 0.14
20 SGTC_1981 st071826 3.19 47.0 µM 6.27E-4 12 7.21E-4 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.098 1.47E-8 YML075C HMG1 hom HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress
0.090 1.88E-7 YOR157C PUP1 het Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
0.090 1.94E-7 YIL139C REV7 hom Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
0.087 4.40E-7 YDR409W SIZ1 hom SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring
0.085 8.38E-7 YGR133W PEX4 hom Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis
0.083 1.30E-6 YPL182C_d YPL182C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W
0.080 3.69E-6 YGL006W-A_p YGL006W-A_p hom Putative protein of unknown function; identified by SAGE
0.079 4.81E-6 YLL011W SOF1 het Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p
0.079 4.87E-6 YOL026C MIM1 het Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex
0.078 5.43E-6 YGL074C_d YGL074C_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
0.075 1.38E-5 YGR245C SDA1 het Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis
0.074 1.82E-5 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.074 1.94E-5 YJR048W CYC1 hom Cytochrome c, isoform 1; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication
0.073 2.12E-5 YDR043C NRG1 hom Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; NRG1 has a paralog, NRG2, that arose from the whole genome duplication
0.073 2.15E-5 YOR105W_p YOR105W_p hom Protein of unknown function; expressed at both mRNA and protein levels