HIPHOP chemogenomics database

YBL078C / ATG8

Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis

Zygosity: Homozygous strain

fixedexpanded
Profile for YBL078C / ATG8

Click on Significant Values for Screen Details

Top fitness defect scores for YBL078C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1685	st024049	8.65	53.1 µM	2.79E-16	TSC3-RPN4	2	2.66E-18
2	SGTC_2856	9022925	7.48	19.5 µM	1.31E-12		2	3.61E-14
3	SGTC_2370	9071495	7.40	200.0 µM	2.32E-12	mitochondrial processes	3	6.88E-14
4	SGTC_3071	9117417	7.08	49.5 µM	1.87E-11	fatty acid desaturase (OLE1)	4	7.24E-13
5	SGTC_1649	st011737	6.50	51.3 µM	6.65E-10		14	4.05E-11
6	SGTC_2485	5633444	6.27	17.8 µM	2.49E-9	redox potentiating	11	1.80E-10
7	SGTC_1868	st057644	6.15	42.7 µM	5.05E-9		8	3.99E-10
8	SGTC_934	2950-0684	6.12	338.0 µM	5.68E-9		5	4.55E-10
9	SGTC_2643	anethole	5.37	100.0 µM	2.92E-7		4	3.89E-8
10	SGTC_378	3970-0959	5.22	8.3 µM	6.02E-7		5	8.83E-8
11	SGTC_1533	dihydroergotamine mesylate	5.09	29.4 µM	1.12E-6	DNA intercalators	5	1.79E-7
12	SGTC_936	3005-4620	4.95	401.0 µM	2.13E-6		10	3.67E-7
13	SGTC_1835	st008666	4.84	49.2 µM	3.53E-6		12	6.51E-7
14	SGTC_2213	7173844	4.71	200.0 µM	6.29E-6		7	1.25E-6
15	SGTC_780	0109-0199	4.66	192.0 µM	7.83E-6		5	1.60E-6
16	SGTC_2795	7780010	4.58	54.5 µM	1.11E-5		13	2.37E-6
17	SGTC_1726	st035556	4.55	14.6 µM	1.24E-5		24	2.69E-6
18	SGTC_3214	9129694	4.54	49.5 µM	1.31E-5		12	2.87E-6
19	SGTC_1184	1269-1718	4.44	139.0 µM	1.91E-5		16	4.40E-6
20	SGTC_2183	6420625	4.40	130.5 µM	2.32E-5	mitochondrial processes	38	5.47E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.086	6.70E-7	YOR385W_p	YOR385W_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene
0.085	7.80E-7	YBR159W	IFA38	hom	Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides
0.082	2.17E-6	YNR054C	ESF2	het	Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome
0.079	4.78E-6	YOR266W	PNT1	hom	Mitochondrial integral inner membrane protein involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine
0.077	8.49E-6	YMR194C-A_d	YMR194C-A_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.075	1.26E-5	YCL030C	HIS4	hom	Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis
0.073	2.40E-5	YDL015C	TSC13	het	Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress
0.072	3.27E-5	YPL025C_d	YPL025C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.069	6.77E-5	YEL071W	DLD3	hom	D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm
0.068	8.91E-5	YKL069W	YKL069W	hom	Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress
0.068	9.01E-5	YPR095C	SYT1	hom	Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain
0.067	1.05E-4	YKR021W	ALY1	hom	Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication
0.065	1.59E-4	YBR007C	DSF2	hom	Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.065	1.84E-4	YBR027C_d	YBR027C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.065	1.84E-4	YBR056W-A_p	YBR056W-A_p	hom	Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B