YBL082C / ALG3

Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins

Zygosity: Homozygous strain
fixedexpanded
Profile for YBL082C / ALG3

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-4.49|P-value:5.36E-7 ID:SGTC_143|Compound:4032-1194|FD-Score:5.26|P-value:5.67E-9 ID:SGTC_160|Compound:3453-0671|FD-Score:-2.90|P-value:8.35E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.02|P-value:5.25E-4 ID:SGTC_294|Compound:k038-0071|FD-Score:2.88|P-value:8.87E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-2.98|P-value:6.15E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.30|P-value:1.55E-6 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.37|P-value:2.80E-9 ID:SGTC_324|Compound:1315-0088|FD-Score:-3.65|P-value:3.79E-5 ID:SGTC_327|Compound:1273-0057|FD-Score:-5.09|P-value:1.69E-8 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:4.81|P-value:8.85E-8 ID:SGTC_411|Compound:enrofloxacin|FD-Score:-2.95|P-value:6.77E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:2.88|P-value:9.02E-4 ID:SGTC_495|Compound:decylubiquinone|FD-Score:3.53|P-value:6.55E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:5.11|P-value:1.49E-8 ID:SGTC_549|Compound:1661-1316|FD-Score:3.22|P-value:2.36E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:6.59|P-value:4.51E-13 ID:SGTC_631|Compound:k072-0230|FD-Score:-2.87|P-value:9.32E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:-2.86|P-value:9.44E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:-3.49|P-value:7.45E-5 ID:SGTC_708|Compound:3448-5400|FD-Score:3.54|P-value:6.08E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:2.85|P-value:9.86E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:-3.56|P-value:5.55E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-6.72|P-value:1.52E-13 ID:SGTC_1126|Compound:isoteolin|FD-Score:-3.01|P-value:5.32E-4 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.38|P-value:1.22E-4 ID:SGTC_1474|Compound:0330-0125|FD-Score:-3.14|P-value:3.22E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.48|P-value:8.07E-5 ID:SGTC_1754|Compound:st042065|FD-Score:10.50|P-value:1.75E-30 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.21|P-value:2.50E-4 ID:SGTC_1991|Compound:st073000|FD-Score:3.32|P-value:1.57E-4 ID:SGTC_2021|Compound:4019055|FD-Score:5.72|P-value:2.76E-10 ID:SGTC_2070|Compound:5230947|FD-Score:-4.31|P-value:1.47E-6 ID:SGTC_2157|Compound:5649910|FD-Score:4.29|P-value:1.68E-6 ID:SGTC_2266|Compound:7929956|FD-Score:-3.17|P-value:2.87E-4 ID:SGTC_2268|Compound:7933382|FD-Score:-3.19|P-value:2.70E-4 ID:SGTC_2269|Compound:7935389|FD-Score:-3.02|P-value:5.11E-4 ID:SGTC_2272|Compound:7805186|FD-Score:4.83|P-value:8.28E-8 ID:SGTC_2289|Compound:7946404|FD-Score:4.37|P-value:1.07E-6 ID:SGTC_2399|Compound:5884133|FD-Score:3.00|P-value:5.61E-4 ID:SGTC_2413|Compound:inermin|FD-Score:5.51|P-value:1.14E-9 ID:SGTC_2487|Compound:5918373|FD-Score:3.65|P-value:3.76E-5 ID:SGTC_2623|Compound:humulene|FD-Score:3.23|P-value:2.33E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:4.10|P-value:4.39E-6 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.36|P-value:3.04E-9 ID:SGTC_2714|Compound:st079313|FD-Score:-2.96|P-value:6.54E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:-2.95|P-value:6.86E-4 ID:SGTC_2821|Compound:7996764|FD-Score:4.01|P-value:6.90E-6 ID:SGTC_2833|Compound:9001315|FD-Score:3.70|P-value:2.93E-5 ID:SGTC_2834|Compound:9001755|FD-Score:-3.53|P-value:6.35E-5 ID:SGTC_2922|Compound:7999257|FD-Score:-4.48|P-value:5.77E-7 ID:SGTC_2941|Compound:9056145|FD-Score:2.89|P-value:8.52E-4 ID:SGTC_3014|Compound:9081533|FD-Score:2.90|P-value:8.31E-4 ID:SGTC_3037|Compound:9091026|FD-Score:5.17|P-value:1.03E-8 ID:SGTC_3152|Compound:9099253|FD-Score:3.84|P-value:1.57E-5 ID:SGTC_3210|Compound:9128157|FD-Score:3.59|P-value:5.02E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.49|P-value:5.36E-7 ID:SGTC_143|Compound:4032-1194|FD-Score:5.26|P-value:5.67E-9 ID:SGTC_160|Compound:3453-0671|FD-Score:-2.90|P-value:8.35E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.02|P-value:5.25E-4 ID:SGTC_294|Compound:k038-0071|FD-Score:2.88|P-value:8.87E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-2.98|P-value:6.15E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.30|P-value:1.55E-6 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.37|P-value:2.80E-9 ID:SGTC_324|Compound:1315-0088|FD-Score:-3.65|P-value:3.79E-5 ID:SGTC_327|Compound:1273-0057|FD-Score:-5.09|P-value:1.69E-8 ID:SGTC_410|Compound:α-linolenic acid|FD-Score:4.81|P-value:8.85E-8 ID:SGTC_411|Compound:enrofloxacin|FD-Score:-2.95|P-value:6.77E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:2.88|P-value:9.02E-4 ID:SGTC_495|Compound:decylubiquinone|FD-Score:3.53|P-value:6.55E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:5.11|P-value:1.49E-8 ID:SGTC_549|Compound:1661-1316|FD-Score:3.22|P-value:2.36E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:6.59|P-value:4.51E-13 ID:SGTC_631|Compound:k072-0230|FD-Score:-2.87|P-value:9.32E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:-2.86|P-value:9.44E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:-3.49|P-value:7.45E-5 ID:SGTC_708|Compound:3448-5400|FD-Score:3.54|P-value:6.08E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:2.85|P-value:9.86E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:-3.56|P-value:5.55E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-6.72|P-value:1.52E-13 ID:SGTC_1126|Compound:isoteolin|FD-Score:-3.01|P-value:5.32E-4 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.38|P-value:1.22E-4 ID:SGTC_1474|Compound:0330-0125|FD-Score:-3.14|P-value:3.22E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.48|P-value:8.07E-5 ID:SGTC_1754|Compound:st042065|FD-Score:10.50|P-value:1.75E-30 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.21|P-value:2.50E-4 ID:SGTC_1991|Compound:st073000|FD-Score:3.32|P-value:1.57E-4 ID:SGTC_2021|Compound:4019055|FD-Score:5.72|P-value:2.76E-10 ID:SGTC_2070|Compound:5230947|FD-Score:-4.31|P-value:1.47E-6 ID:SGTC_2157|Compound:5649910|FD-Score:4.29|P-value:1.68E-6 ID:SGTC_2266|Compound:7929956|FD-Score:-3.17|P-value:2.87E-4 ID:SGTC_2268|Compound:7933382|FD-Score:-3.19|P-value:2.70E-4 ID:SGTC_2269|Compound:7935389|FD-Score:-3.02|P-value:5.11E-4 ID:SGTC_2272|Compound:7805186|FD-Score:4.83|P-value:8.28E-8 ID:SGTC_2289|Compound:7946404|FD-Score:4.37|P-value:1.07E-6 ID:SGTC_2399|Compound:5884133|FD-Score:3.00|P-value:5.61E-4 ID:SGTC_2413|Compound:inermin|FD-Score:5.51|P-value:1.14E-9 ID:SGTC_2487|Compound:5918373|FD-Score:3.65|P-value:3.76E-5 ID:SGTC_2623|Compound:humulene|FD-Score:3.23|P-value:2.33E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:4.10|P-value:4.39E-6 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.36|P-value:3.04E-9 ID:SGTC_2714|Compound:st079313|FD-Score:-2.96|P-value:6.54E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:-2.95|P-value:6.86E-4 ID:SGTC_2821|Compound:7996764|FD-Score:4.01|P-value:6.90E-6 ID:SGTC_2833|Compound:9001315|FD-Score:3.70|P-value:2.93E-5 ID:SGTC_2834|Compound:9001755|FD-Score:-3.53|P-value:6.35E-5 ID:SGTC_2922|Compound:7999257|FD-Score:-4.48|P-value:5.77E-7 ID:SGTC_2941|Compound:9056145|FD-Score:2.89|P-value:8.52E-4 ID:SGTC_3014|Compound:9081533|FD-Score:2.90|P-value:8.31E-4 ID:SGTC_3037|Compound:9091026|FD-Score:5.17|P-value:1.03E-8 ID:SGTC_3152|Compound:9099253|FD-Score:3.84|P-value:1.57E-5 ID:SGTC_3210|Compound:9128157|FD-Score:3.59|P-value:5.02E-5

Top fitness defect scores for YBL082C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1754 st042065 10.50 16.1 µM 1.75E-30 NEO1-PIK1 20 3.41E-26
2 SGTC_625 k213-0058 6.59 121.0 µM 4.51E-13 translation 26 2.26E-11
3 SGTC_2021 4019055 5.72 173.0 µM 2.76E-10 endomembrane recycling 44 5.36E-9
4 SGTC_2413 inermin 5.51 26.1 µM 1.14E-9 22 1.80E-8
5 SGTC_2688 kanamycin b 5.36 96.7 µM 3.04E-9 calcium & mitochondrial duress 210 4.15E-8
6 SGTC_143 4032-1194 5.26 15.7 µM 5.67E-9 26 7.07E-8
7 SGTC_3037 9091026 5.17 49.5 µM 1.03E-8 NEO1-PIK1 72 1.18E-7
8 SGTC_513 4245-1575 5.11 41.7 µM 1.49E-8 unfolded protein response 69 1.61E-7
9 SGTC_2272 7805186 4.83 180.0 µM 8.28E-8 cell wall signaling 12 6.97E-7
10 SGTC_410 α-linolenic acid 4.81 100.0 µM 8.85E-8 excess fatty acid 20 7.38E-7
11 SGTC_2289 7946404 4.37 165.1 µM 1.07E-6 77 6.22E-6
12 SGTC_2157 5649910 4.29 96.4 µM 1.68E-6 16 9.13E-6
13 SGTC_2629 bisabolol 4.10 100.0 µM 4.39E-6 17 2.08E-5
14 SGTC_2821 7996764 4.01 71.4 µM 6.90E-6 33 3.06E-5
15 SGTC_3152 9099253 3.84 49.5 µM 1.57E-5 NEO1-PIK1 136 6.20E-5
16 SGTC_2833 9001315 3.70 45.5 µM 2.93E-5 amide catabolism 67 1.06E-4
17 SGTC_2487 5918373 3.65 44.0 µM 3.76E-5 22 1.31E-4
18 SGTC_3210 9128157 3.59 49.5 µM 5.02E-5 63 1.68E-4
19 SGTC_708 3448-5400 3.54 146.0 µM 6.08E-5 59 1.97E-4
20 SGTC_495 decylubiquinone 3.53 155.0 µM 6.55E-5 copper-dependent oxidative stress 39 2.11E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.291 1.62E-66 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.265 6.53E-55 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.263 3.43E-54 YGR202C PCT1 hom Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity
0.248 2.27E-48 YNL219C ALG9 hom Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
0.239 8.03E-45 YGR166W TRS65 hom Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.223 6.25E-39 YOR002W ALG6 hom Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease
0.208 3.26E-34 YBL083C_d YBL083C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3
0.203 1.93E-32 YLR360W VPS38 hom Part of a Vps34p phosphatidylinositol 3-kinase complex that functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated
0.193 2.11E-29 YDR084C TVP23 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.191 5.05E-29 YDR508C GNP1 hom High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication
0.188 5.87E-28 YML106W URA5 hom Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication
0.187 8.28E-28 YEL048C TCA17 hom Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder
0.184 5.44E-27 YNL294C RIM21 hom pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH
0.179 1.20E-25 YGL043W DST1 hom General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress
0.176 9.09E-25 YPL120W VPS30 hom Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1