YBL104C / SEA4

Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites

Zygosity: Homozygous strain
fixedexpanded
Profile for YBL104C / SEA4

Click on Significant Values for Screen Details ID:SGTC_101|Compound:0490-5701|FD-Score:5.16|P-value:2.65E-7 ID:SGTC_117|Compound:lucigenin|FD-Score:5.38|P-value:8.14E-8 ID:SGTC_462|Compound:ag-879|FD-Score:3.45|P-value:5.52E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:4.10|P-value:4.38E-5 ID:SGTC_572|Compound:0960-0087|FD-Score:4.72|P-value:2.49E-6 ID:SGTC_584|Compound:0986-0246|FD-Score:4.45|P-value:9.28E-6 ID:SGTC_692|Compound:k241-0027|FD-Score:6.46|P-value:1.11E-10 ID:SGTC_701|Compound:4424-0219|FD-Score:-3.28|P-value:1.31E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:5.05|P-value:4.80E-7 ID:SGTC_942|Compound:3253-1115|FD-Score:3.31|P-value:9.28E-4 ID:SGTC_972|Compound:1319-0219|FD-Score:4.31|P-value:1.73E-5 ID:SGTC_1015|Compound:4130-4525|FD-Score:3.55|P-value:3.85E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.47|P-value:5.22E-4 ID:SGTC_1064|Compound:honokiol|FD-Score:4.75|P-value:2.21E-6 ID:SGTC_1066|Compound:ketoconazole|FD-Score:5.44|P-value:5.61E-8 ID:SGTC_1081|Compound:sertraline|FD-Score:5.09|P-value:3.78E-7 ID:SGTC_1085|Compound:doxorubicin|FD-Score:3.49|P-value:4.85E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:4.01|P-value:6.26E-5 ID:SGTC_1119|Compound:parthenolide|FD-Score:-3.25|P-value:1.49E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.38|P-value:8.85E-5 ID:SGTC_1134|Compound:3477-0009|FD-Score:4.26|P-value:2.15E-5 ID:SGTC_1179|Compound:1021-1431|FD-Score:3.59|P-value:3.33E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:4.56|P-value:5.50E-6 ID:SGTC_1279|Compound:0874-0590|FD-Score:-3.09|P-value:2.75E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:-5.36|P-value:3.64E-9 ID:SGTC_1386|Compound:3228-0095|FD-Score:4.26|P-value:2.15E-5 ID:SGTC_1387|Compound:3342-1166|FD-Score:5.59|P-value:2.47E-8 ID:SGTC_1388|Compound:3346-4487|FD-Score:-3.19|P-value:1.91E-4 ID:SGTC_1395|Compound:3486-0190|FD-Score:-4.66|P-value:2.00E-7 ID:SGTC_1480|Compound:1070-0044|FD-Score:5.34|P-value:9.88E-8 ID:SGTC_1559|Compound:coumarin 343|FD-Score:4.89|P-value:1.08E-6 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:3.35|P-value:7.93E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.90|P-value:1.01E-4 ID:SGTC_1571|Compound:st070080|FD-Score:3.39|P-value:6.87E-4 ID:SGTC_1632|Compound:st005650|FD-Score:4.18|P-value:3.07E-5 ID:SGTC_1675|Compound:st018485|FD-Score:5.49|P-value:4.30E-8 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.96|P-value:7.64E-5 ID:SGTC_1876|Compound:myriocin|FD-Score:4.61|P-value:4.30E-6 ID:SGTC_1920|Compound:st075728|FD-Score:3.70|P-value:2.19E-4 ID:SGTC_1980|Compound:st071811|FD-Score:4.30|P-value:1.77E-5 ID:SGTC_2017|Compound:5132927|FD-Score:4.57|P-value:5.26E-6 ID:SGTC_2078|Compound:5212016|FD-Score:-3.25|P-value:1.48E-4 ID:SGTC_2137|Compound:5261020|FD-Score:4.50|P-value:7.35E-6 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:4.00|P-value:6.60E-5 ID:SGTC_2313|Compound:7726885|FD-Score:5.21|P-value:2.06E-7 ID:SGTC_2332|Compound:7982454|FD-Score:4.51|P-value:6.92E-6 ID:SGTC_2376|Compound:9072922|FD-Score:4.86|P-value:1.26E-6 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:4.27|P-value:2.03E-5 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.34|P-value:8.37E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:4.42|P-value:1.05E-5 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:3.44|P-value:5.69E-4 ID:SGTC_2720|Compound:fluspirilene|FD-Score:3.45|P-value:5.53E-4 ID:SGTC_2747|Compound:doxorubicin|FD-Score:3.70|P-value:2.22E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:4.68|P-value:2.99E-6 ID:SGTC_2910|Compound:7948405|FD-Score:3.53|P-value:4.19E-4 ID:SGTC_3061|Compound:glucosamine|FD-Score:3.97|P-value:7.34E-5 ID:SGTC_3126|Compound:9127922|FD-Score:-2.85|P-value:6.66E-4 ID:SGTC_3173|Compound:9105693|FD-Score:4.33|P-value:1.55E-5 ID:SGTC_3187|Compound:9109394|FD-Score:5.76|P-value:9.25E-9 ID:SGTC_3217|Compound:9129673|FD-Score:4.98|P-value:6.78E-7 ID:SGTC_3238|Compound:9133470|FD-Score:4.14|P-value:3.61E-5 ID:SGTC_3242|Compound:9133940|FD-Score:3.95|P-value:7.95E-5 ID:SGTC_3293|Compound:9120939|FD-Score:11.90|P-value:2.33E-33 ID:SGTC_3294|Compound:9122953|FD-Score:3.96|P-value:7.84E-5 ID:SGTC_101|Compound:0490-5701|FD-Score:5.16|P-value:2.65E-7 ID:SGTC_117|Compound:lucigenin|FD-Score:5.38|P-value:8.14E-8 ID:SGTC_462|Compound:ag-879|FD-Score:3.45|P-value:5.52E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:4.10|P-value:4.38E-5 ID:SGTC_572|Compound:0960-0087|FD-Score:4.72|P-value:2.49E-6 ID:SGTC_584|Compound:0986-0246|FD-Score:4.45|P-value:9.28E-6 ID:SGTC_692|Compound:k241-0027|FD-Score:6.46|P-value:1.11E-10 ID:SGTC_701|Compound:4424-0219|FD-Score:-3.28|P-value:1.31E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:5.05|P-value:4.80E-7 ID:SGTC_942|Compound:3253-1115|FD-Score:3.31|P-value:9.28E-4 ID:SGTC_972|Compound:1319-0219|FD-Score:4.31|P-value:1.73E-5 ID:SGTC_1015|Compound:4130-4525|FD-Score:3.55|P-value:3.85E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.47|P-value:5.22E-4 ID:SGTC_1064|Compound:honokiol|FD-Score:4.75|P-value:2.21E-6 ID:SGTC_1066|Compound:ketoconazole|FD-Score:5.44|P-value:5.61E-8 ID:SGTC_1081|Compound:sertraline|FD-Score:5.09|P-value:3.78E-7 ID:SGTC_1085|Compound:doxorubicin|FD-Score:3.49|P-value:4.85E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:4.01|P-value:6.26E-5 ID:SGTC_1119|Compound:parthenolide|FD-Score:-3.25|P-value:1.49E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:-3.38|P-value:8.85E-5 ID:SGTC_1134|Compound:3477-0009|FD-Score:4.26|P-value:2.15E-5 ID:SGTC_1179|Compound:1021-1431|FD-Score:3.59|P-value:3.33E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:4.56|P-value:5.50E-6 ID:SGTC_1279|Compound:0874-0590|FD-Score:-3.09|P-value:2.75E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:-5.36|P-value:3.64E-9 ID:SGTC_1386|Compound:3228-0095|FD-Score:4.26|P-value:2.15E-5 ID:SGTC_1387|Compound:3342-1166|FD-Score:5.59|P-value:2.47E-8 ID:SGTC_1388|Compound:3346-4487|FD-Score:-3.19|P-value:1.91E-4 ID:SGTC_1395|Compound:3486-0190|FD-Score:-4.66|P-value:2.00E-7 ID:SGTC_1480|Compound:1070-0044|FD-Score:5.34|P-value:9.88E-8 ID:SGTC_1559|Compound:coumarin 343|FD-Score:4.89|P-value:1.08E-6 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:3.35|P-value:7.93E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.90|P-value:1.01E-4 ID:SGTC_1571|Compound:st070080|FD-Score:3.39|P-value:6.87E-4 ID:SGTC_1632|Compound:st005650|FD-Score:4.18|P-value:3.07E-5 ID:SGTC_1675|Compound:st018485|FD-Score:5.49|P-value:4.30E-8 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.96|P-value:7.64E-5 ID:SGTC_1876|Compound:myriocin|FD-Score:4.61|P-value:4.30E-6 ID:SGTC_1920|Compound:st075728|FD-Score:3.70|P-value:2.19E-4 ID:SGTC_1980|Compound:st071811|FD-Score:4.30|P-value:1.77E-5 ID:SGTC_2017|Compound:5132927|FD-Score:4.57|P-value:5.26E-6 ID:SGTC_2078|Compound:5212016|FD-Score:-3.25|P-value:1.48E-4 ID:SGTC_2137|Compound:5261020|FD-Score:4.50|P-value:7.35E-6 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:4.00|P-value:6.60E-5 ID:SGTC_2313|Compound:7726885|FD-Score:5.21|P-value:2.06E-7 ID:SGTC_2332|Compound:7982454|FD-Score:4.51|P-value:6.92E-6 ID:SGTC_2376|Compound:9072922|FD-Score:4.86|P-value:1.26E-6 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:4.27|P-value:2.03E-5 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.34|P-value:8.37E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:4.42|P-value:1.05E-5 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:3.44|P-value:5.69E-4 ID:SGTC_2720|Compound:fluspirilene|FD-Score:3.45|P-value:5.53E-4 ID:SGTC_2747|Compound:doxorubicin|FD-Score:3.70|P-value:2.22E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:4.68|P-value:2.99E-6 ID:SGTC_2910|Compound:7948405|FD-Score:3.53|P-value:4.19E-4 ID:SGTC_3061|Compound:glucosamine|FD-Score:3.97|P-value:7.34E-5 ID:SGTC_3126|Compound:9127922|FD-Score:-2.85|P-value:6.66E-4 ID:SGTC_3173|Compound:9105693|FD-Score:4.33|P-value:1.55E-5 ID:SGTC_3187|Compound:9109394|FD-Score:5.76|P-value:9.25E-9 ID:SGTC_3217|Compound:9129673|FD-Score:4.98|P-value:6.78E-7 ID:SGTC_3238|Compound:9133470|FD-Score:4.14|P-value:3.61E-5 ID:SGTC_3242|Compound:9133940|FD-Score:3.95|P-value:7.95E-5 ID:SGTC_3293|Compound:9120939|FD-Score:11.90|P-value:2.33E-33 ID:SGTC_3294|Compound:9122953|FD-Score:3.96|P-value:7.84E-5

Top fitness defect scores for YBL104C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3293 9120939 11.90 62.0 µM 2.33E-33 1 3.50E-33
2 SGTC_692 k241-0027 6.46 77.8 µM 1.11E-10 1 5.35E-11
3 SGTC_3187 9109394 5.76 49.5 µM 9.25E-9 Golgi 4 4.33E-9
4 SGTC_1387 3342-1166 5.59 1.8 µM 2.47E-8 mitochondrial processes 2 1.15E-8
5 SGTC_1675 st018485 5.49 24.5 µM 4.30E-8 60S ribosome export 9 2.00E-8
6 SGTC_1066 ketoconazole 5.44 628.0 nM 5.61E-8 2 2.61E-8
7 SGTC_117 lucigenin 5.38 22.1 µM 8.14E-8 redox potentiating 50 3.79E-8
8 SGTC_1480 1070-0044 5.34 41.2 µM 9.88E-8 2 4.60E-8
9 SGTC_2313 7726885 5.21 200.0 µM 2.06E-7 RPP1 & pyrimidine depletion 13 9.58E-8
10 SGTC_101 0490-5701 5.16 117.5 µM 2.65E-7 1 1.23E-7
11 SGTC_1081 sertraline 5.09 6.0 µM 3.78E-7 60S ribosome export 15 1.76E-7
12 SGTC_841 0251-0215 5.05 91.2 µM 4.80E-7 53 2.23E-7
13 SGTC_3217 9129673 4.98 49.5 µM 6.78E-7 11 3.16E-7
14 SGTC_1559 coumarin 343 4.89 70.1 µM 1.08E-6 3 5.03E-7
15 SGTC_2376 9072922 4.86 81.3 µM 1.26E-6 11 5.89E-7
16 SGTC_1064 honokiol 4.75 6.8 µM 2.21E-6 12 1.04E-6
17 SGTC_572 0960-0087 4.72 17.7 µM 2.49E-6 14 1.16E-6
18 SGTC_2760 etidronate 4.68 98.0 µM 2.99E-6 13 1.40E-6
19 SGTC_1876 myriocin 4.61 605.0 nM 4.30E-6 24 2.02E-6
20 SGTC_2017 5132927 4.57 60.6 µM 5.26E-6 16 2.47E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.118 8.12E-12 YGR212W SLI1 hom N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin
0.097 1.73E-8 YBL020W RFT1 het Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein
0.094 5.08E-8 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.093 6.85E-8 YDL239C ADY3 hom Protein required for spore wall formation; thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication
0.084 1.12E-6 YGL112C TAF6 het Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4
0.084 1.25E-6 YHR032W ERC1 hom Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine
0.079 4.81E-6 YKR009C FOX2 hom Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities
0.079 5.18E-6 YEL003W GIM4 hom Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it
0.079 5.04E-6 YBL069W AST1 hom Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication
0.072 2.89E-5 YOR083W WHI5 hom Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1
0.071 3.41E-5 YMR296C LCB1 het Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
0.071 4.29E-5 YKR071C DRE2 het Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
0.070 5.20E-5 YBR073W RDH54 hom DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p
0.069 7.06E-5 YLR108C YLR108C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication
0.066 1.20E-4 YHR136C SPL2 hom Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm