YBR015C / MNN2

Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR015C / MNN2

Click on Significant Values for Screen Details ID:SGTC_130|Compound:0136-0219|FD-Score:-3.55|P-value:1.62E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:4.84|P-value:5.16E-6 ID:SGTC_146|Compound:3448-8650|FD-Score:8.11|P-value:2.43E-14 ID:SGTC_154|Compound:3389-1638|FD-Score:6.14|P-value:7.76E-9 ID:SGTC_161|Compound:4065-0411|FD-Score:4.78|P-value:6.77E-6 ID:SGTC_439|Compound:amiodarone|FD-Score:3.98|P-value:1.64E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:7.66|P-value:5.88E-13 ID:SGTC_469|Compound:w7|FD-Score:3.47|P-value:9.41E-4 ID:SGTC_486|Compound:niguldipine|FD-Score:3.75|P-value:3.78E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:5.08|P-value:1.75E-6 ID:SGTC_500|Compound:h-89|FD-Score:9.40|P-value:9.52E-19 ID:SGTC_504|Compound:lovastatin|FD-Score:4.03|P-value:1.36E-4 ID:SGTC_507|Compound:nimesulide|FD-Score:3.99|P-value:1.58E-4 ID:SGTC_511|Compound:blebbistatin|FD-Score:6.69|P-value:3.20E-10 ID:SGTC_522|Compound:1273-0010|FD-Score:5.03|P-value:2.19E-6 ID:SGTC_540|Compound:1187-0821|FD-Score:6.22|P-value:4.98E-9 ID:SGTC_542|Compound:3381-0244|FD-Score:-3.11|P-value:7.54E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:3.56|P-value:7.08E-4 ID:SGTC_687|Compound:k029-0044|FD-Score:3.56|P-value:7.07E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:3.72|P-value:4.19E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:3.48|P-value:9.25E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:4.18|P-value:7.79E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:4.14|P-value:9.04E-5 ID:SGTC_831|Compound:k061-1017|FD-Score:7.20|P-value:1.31E-11 ID:SGTC_856|Compound:0433-0016|FD-Score:3.55|P-value:7.29E-4 ID:SGTC_889|Compound:0919-1524|FD-Score:3.84|P-value:2.74E-4 ID:SGTC_896|Compound:0964-0007|FD-Score:4.13|P-value:9.27E-5 ID:SGTC_981|Compound:1488-1094|FD-Score:5.30|P-value:6.21E-7 ID:SGTC_987|Compound:1497-1013|FD-Score:4.30|P-value:4.81E-5 ID:SGTC_1013|Compound:4112-3890|FD-Score:3.79|P-value:3.30E-4 ID:SGTC_1032|Compound:k061-1018|FD-Score:4.35|P-value:4.02E-5 ID:SGTC_1074|Compound:carmofur|FD-Score:4.65|P-value:1.16E-5 ID:SGTC_1085|Compound:doxorubicin|FD-Score:4.21|P-value:6.85E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:4.63|P-value:1.25E-5 ID:SGTC_1250|Compound:0485-0349|FD-Score:4.55|P-value:1.79E-5 ID:SGTC_1337|Compound:1486-1011|FD-Score:4.85|P-value:4.92E-6 ID:SGTC_1402|Compound:3909-7673|FD-Score:5.66|P-value:1.04E-7 ID:SGTC_1487|Compound:3970-0795|FD-Score:3.62|P-value:5.77E-4 ID:SGTC_1534|Compound:6-azauridine|FD-Score:3.90|P-value:2.17E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.88|P-value:2.32E-4 ID:SGTC_1632|Compound:st005650|FD-Score:4.04|P-value:1.31E-4 ID:SGTC_1633|Compound:st005664|FD-Score:3.50|P-value:8.51E-4 ID:SGTC_1637|Compound:st007924|FD-Score:5.15|P-value:1.26E-6 ID:SGTC_1760|Compound:st045453|FD-Score:4.05|P-value:1.25E-4 ID:SGTC_1917|Compound:st066507|FD-Score:5.37|P-value:4.31E-7 ID:SGTC_1943|Compound:st076331|FD-Score:5.28|P-value:6.61E-7 ID:SGTC_2035|Compound:5154463|FD-Score:4.24|P-value:6.12E-5 ID:SGTC_2067|Compound:5224629|FD-Score:4.13|P-value:9.48E-5 ID:SGTC_2088|Compound:5278280|FD-Score:4.29|P-value:4.98E-5 ID:SGTC_2092|Compound:5303694|FD-Score:4.25|P-value:5.95E-5 ID:SGTC_2168|Compound:5727979|FD-Score:8.76|P-value:1.75E-16 ID:SGTC_2172|Compound:5754775|FD-Score:5.66|P-value:1.03E-7 ID:SGTC_2177|Compound:5811945|FD-Score:3.92|P-value:2.03E-4 ID:SGTC_2178|Compound:5812956|FD-Score:4.82|P-value:5.65E-6 ID:SGTC_2334|Compound:9017922|FD-Score:4.68|P-value:1.02E-5 ID:SGTC_2381|Compound:9035592|FD-Score:4.10|P-value:1.06E-4 ID:SGTC_2399|Compound:5884133|FD-Score:6.47|P-value:1.22E-9 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:6.19|P-value:5.87E-9 ID:SGTC_2623|Compound:humulene|FD-Score:4.43|P-value:2.90E-5 ID:SGTC_2657|Compound:nystatin|FD-Score:3.60|P-value:6.14E-4 ID:SGTC_2678|Compound:equilin|FD-Score:3.60|P-value:6.29E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.63|P-value:5.65E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:7.47|P-value:2.25E-12 ID:SGTC_2792|Compound:7535235|FD-Score:7.38|P-value:3.99E-12 ID:SGTC_2870|Compound:9038528|FD-Score:5.19|P-value:1.02E-6 ID:SGTC_2873|Compound:9040596|FD-Score:7.85|P-value:1.60E-13 ID:SGTC_2874|Compound:9043904|FD-Score:3.49|P-value:8.91E-4 ID:SGTC_2911|Compound:7942208|FD-Score:4.38|P-value:3.49E-5 ID:SGTC_2930|Compound:9013230|FD-Score:-5.32|P-value:6.15E-8 ID:SGTC_2934|Compound:9036370|FD-Score:8.06|P-value:3.58E-14 ID:SGTC_3123|Compound:9126162|FD-Score:3.46|P-value:9.95E-4 ID:SGTC_3301|Compound:9125354|FD-Score:7.39|P-value:3.75E-12 ID:SGTC_3303|Compound:9125618|FD-Score:6.33|P-value:2.75E-9 ID:SGTC_3308|Compound:9128332|FD-Score:4.23|P-value:6.44E-5 ID:SGTC_130|Compound:0136-0219|FD-Score:-3.55|P-value:1.62E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:4.84|P-value:5.16E-6 ID:SGTC_146|Compound:3448-8650|FD-Score:8.11|P-value:2.43E-14 ID:SGTC_154|Compound:3389-1638|FD-Score:6.14|P-value:7.76E-9 ID:SGTC_161|Compound:4065-0411|FD-Score:4.78|P-value:6.77E-6 ID:SGTC_439|Compound:amiodarone|FD-Score:3.98|P-value:1.64E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:7.66|P-value:5.88E-13 ID:SGTC_469|Compound:w7|FD-Score:3.47|P-value:9.41E-4 ID:SGTC_486|Compound:niguldipine|FD-Score:3.75|P-value:3.78E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:5.08|P-value:1.75E-6 ID:SGTC_500|Compound:h-89|FD-Score:9.40|P-value:9.52E-19 ID:SGTC_504|Compound:lovastatin|FD-Score:4.03|P-value:1.36E-4 ID:SGTC_507|Compound:nimesulide|FD-Score:3.99|P-value:1.58E-4 ID:SGTC_511|Compound:blebbistatin|FD-Score:6.69|P-value:3.20E-10 ID:SGTC_522|Compound:1273-0010|FD-Score:5.03|P-value:2.19E-6 ID:SGTC_540|Compound:1187-0821|FD-Score:6.22|P-value:4.98E-9 ID:SGTC_542|Compound:3381-0244|FD-Score:-3.11|P-value:7.54E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:3.56|P-value:7.08E-4 ID:SGTC_687|Compound:k029-0044|FD-Score:3.56|P-value:7.07E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:3.72|P-value:4.19E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:3.48|P-value:9.25E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:4.18|P-value:7.79E-5 ID:SGTC_800|Compound:3448-7362|FD-Score:4.14|P-value:9.04E-5 ID:SGTC_831|Compound:k061-1017|FD-Score:7.20|P-value:1.31E-11 ID:SGTC_856|Compound:0433-0016|FD-Score:3.55|P-value:7.29E-4 ID:SGTC_889|Compound:0919-1524|FD-Score:3.84|P-value:2.74E-4 ID:SGTC_896|Compound:0964-0007|FD-Score:4.13|P-value:9.27E-5 ID:SGTC_981|Compound:1488-1094|FD-Score:5.30|P-value:6.21E-7 ID:SGTC_987|Compound:1497-1013|FD-Score:4.30|P-value:4.81E-5 ID:SGTC_1013|Compound:4112-3890|FD-Score:3.79|P-value:3.30E-4 ID:SGTC_1032|Compound:k061-1018|FD-Score:4.35|P-value:4.02E-5 ID:SGTC_1074|Compound:carmofur|FD-Score:4.65|P-value:1.16E-5 ID:SGTC_1085|Compound:doxorubicin|FD-Score:4.21|P-value:6.85E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:4.63|P-value:1.25E-5 ID:SGTC_1250|Compound:0485-0349|FD-Score:4.55|P-value:1.79E-5 ID:SGTC_1337|Compound:1486-1011|FD-Score:4.85|P-value:4.92E-6 ID:SGTC_1402|Compound:3909-7673|FD-Score:5.66|P-value:1.04E-7 ID:SGTC_1487|Compound:3970-0795|FD-Score:3.62|P-value:5.77E-4 ID:SGTC_1534|Compound:6-azauridine|FD-Score:3.90|P-value:2.17E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.88|P-value:2.32E-4 ID:SGTC_1632|Compound:st005650|FD-Score:4.04|P-value:1.31E-4 ID:SGTC_1633|Compound:st005664|FD-Score:3.50|P-value:8.51E-4 ID:SGTC_1637|Compound:st007924|FD-Score:5.15|P-value:1.26E-6 ID:SGTC_1760|Compound:st045453|FD-Score:4.05|P-value:1.25E-4 ID:SGTC_1917|Compound:st066507|FD-Score:5.37|P-value:4.31E-7 ID:SGTC_1943|Compound:st076331|FD-Score:5.28|P-value:6.61E-7 ID:SGTC_2035|Compound:5154463|FD-Score:4.24|P-value:6.12E-5 ID:SGTC_2067|Compound:5224629|FD-Score:4.13|P-value:9.48E-5 ID:SGTC_2088|Compound:5278280|FD-Score:4.29|P-value:4.98E-5 ID:SGTC_2092|Compound:5303694|FD-Score:4.25|P-value:5.95E-5 ID:SGTC_2168|Compound:5727979|FD-Score:8.76|P-value:1.75E-16 ID:SGTC_2172|Compound:5754775|FD-Score:5.66|P-value:1.03E-7 ID:SGTC_2177|Compound:5811945|FD-Score:3.92|P-value:2.03E-4 ID:SGTC_2178|Compound:5812956|FD-Score:4.82|P-value:5.65E-6 ID:SGTC_2334|Compound:9017922|FD-Score:4.68|P-value:1.02E-5 ID:SGTC_2381|Compound:9035592|FD-Score:4.10|P-value:1.06E-4 ID:SGTC_2399|Compound:5884133|FD-Score:6.47|P-value:1.22E-9 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:6.19|P-value:5.87E-9 ID:SGTC_2623|Compound:humulene|FD-Score:4.43|P-value:2.90E-5 ID:SGTC_2657|Compound:nystatin|FD-Score:3.60|P-value:6.14E-4 ID:SGTC_2678|Compound:equilin|FD-Score:3.60|P-value:6.29E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.63|P-value:5.65E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:7.47|P-value:2.25E-12 ID:SGTC_2792|Compound:7535235|FD-Score:7.38|P-value:3.99E-12 ID:SGTC_2870|Compound:9038528|FD-Score:5.19|P-value:1.02E-6 ID:SGTC_2873|Compound:9040596|FD-Score:7.85|P-value:1.60E-13 ID:SGTC_2874|Compound:9043904|FD-Score:3.49|P-value:8.91E-4 ID:SGTC_2911|Compound:7942208|FD-Score:4.38|P-value:3.49E-5 ID:SGTC_2930|Compound:9013230|FD-Score:-5.32|P-value:6.15E-8 ID:SGTC_2934|Compound:9036370|FD-Score:8.06|P-value:3.58E-14 ID:SGTC_3123|Compound:9126162|FD-Score:3.46|P-value:9.95E-4 ID:SGTC_3301|Compound:9125354|FD-Score:7.39|P-value:3.75E-12 ID:SGTC_3303|Compound:9125618|FD-Score:6.33|P-value:2.75E-9 ID:SGTC_3308|Compound:9128332|FD-Score:4.23|P-value:6.44E-5

Top fitness defect scores for YBR015C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_500 h-89 9.40 112.0 µM 9.52E-19 cell wall signaling 5 2.81E-21
2 SGTC_2168 5727979 8.76 200.0 µM 1.75E-16 2 9.80E-19
3 SGTC_146 3448-8650 8.11 53.1 µM 2.43E-14 NEO1-PIK1 26 2.50E-16
4 SGTC_2934 9036370 8.06 36.9 µM 3.58E-14 3 3.87E-16
5 SGTC_2873 9040596 7.85 71.4 µM 1.60E-13 6 2.08E-15
6 SGTC_464 bw-b 70c 7.66 158.0 µM 5.88E-13 1 9.05E-15
7 SGTC_2737 bleomycin 7.47 30.5 nM 2.25E-12 Golgi 21 4.10E-14
8 SGTC_3301 9125354 7.39 19.8 µM 3.75E-12 2 7.29E-14
9 SGTC_2792 7535235 7.38 81.8 µM 3.99E-12 fatty acid desaturase (OLE1) 5 7.83E-14
10 SGTC_831 k061-1017 7.20 217.0 µM 1.31E-11 translation 4 2.99E-13
11 SGTC_511 blebbistatin 6.69 171.0 µM 3.20E-10 DNA intercalators 3 1.11E-11
12 SGTC_2399 5884133 6.47 200.0 µM 1.22E-9 cell wall signaling 26 5.02E-11
13 SGTC_3303 9125618 6.33 40.6 µM 2.75E-9 11 1.26E-10
14 SGTC_540 1187-0821 6.22 17.3 µM 4.98E-9 1 2.47E-10
15 SGTC_2497 7-hydroxyflavone 6.19 100.0 µM 5.87E-9 2 2.97E-10
16 SGTC_154 3389-1638 6.14 511.1 µM 7.76E-9 2 4.07E-10
17 SGTC_2172 5754775 5.66 163.9 µM 1.03E-7 4 7.65E-9
18 SGTC_1402 3909-7673 5.66 35.9 µM 1.04E-7 6 7.76E-9
19 SGTC_1917 st066507 5.37 28.5 µM 4.31E-7 Golgi 16 3.89E-8
20 SGTC_981 1488-1094 5.30 54.9 µM 6.21E-7 8 5.89E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.104 1.40E-9 YML062C MFT1 hom Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
0.093 6.47E-8 YGL084C GUP1 hom Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport
0.090 2.00E-7 YAL027W SAW1 hom 5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
0.088 2.88E-7 YAL053W FLC2 hom Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
0.083 1.41E-6 YAL056C-A_d YAL056C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.082 2.03E-6 YPR092W_d YPR092W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.082 2.06E-6 YNL043C_d YNL043C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W
0.080 3.67E-6 YPL114W_d YPL114W_d hom Dubious open reading frame, unlikely to encode a functional protein; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index
0.079 4.19E-6 YNL056W OCA2 hom Putative protein with similarity to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene
0.079 4.56E-6 YNL246W VPS75 hom NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress
0.078 6.50E-6 YDR372C VPS74 hom Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
0.076 1.01E-5 YFR034C PHO4 hom Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability
0.075 1.51E-5 YJL139C YUR1 hom Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication
0.074 1.96E-5 YBR036C CSG2 hom Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress
0.073 2.17E-5 YLR151C PCD1 hom Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member