YBR017C / KAP104

Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR017C / KAP104

Click on Significant Values for Screen Details ID:SGTC_117|Compound:lucigenin|FD-Score:3.18|P-value:5.36E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:4.65|P-value:9.46E-7 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:3.34|P-value:2.95E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.55|P-value:2.41E-6 ID:SGTC_467|Compound:ro 31-8220|FD-Score:5.07|P-value:1.01E-7 ID:SGTC_611|Compound:3453-1652|FD-Score:3.24|P-value:4.23E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:4.59|P-value:1.25E-6 ID:SGTC_688|Compound:0527-0193|FD-Score:-3.18|P-value:7.21E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:3.58|P-value:1.17E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:4.14|P-value:1.08E-5 ID:SGTC_765|Compound:1319-0117|FD-Score:3.86|P-value:3.69E-5 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.78|P-value:5.17E-5 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.06|P-value:8.20E-4 ID:SGTC_1194|Compound:1488-0194|FD-Score:3.19|P-value:5.05E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:-7.72|P-value:3.24E-15 ID:SGTC_1365|Compound:1672-3266|FD-Score:3.98|P-value:2.17E-5 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.19|P-value:5.16E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.76|P-value:8.51E-7 ID:SGTC_1750|Compound:st081859|FD-Score:3.14|P-value:6.19E-4 ID:SGTC_1764|Compound:st044502|FD-Score:3.15|P-value:5.84E-4 ID:SGTC_1867|Compound:st057372|FD-Score:4.95|P-value:1.94E-7 ID:SGTC_1911|Compound:st060208|FD-Score:3.35|P-value:2.82E-4 ID:SGTC_1921|Compound:5740136|FD-Score:-3.43|P-value:2.96E-4 ID:SGTC_1968|Compound:st069590|FD-Score:3.09|P-value:7.31E-4 ID:SGTC_1975|Compound:st070967|FD-Score:-3.57|P-value:1.72E-4 ID:SGTC_1976|Compound:st070609|FD-Score:-4.27|P-value:8.86E-6 ID:SGTC_2012|Compound:4023501|FD-Score:3.14|P-value:6.08E-4 ID:SGTC_2038|Compound:4100038|FD-Score:3.80|P-value:4.81E-5 ID:SGTC_2205|Compound:7238770|FD-Score:-3.88|P-value:5.00E-5 ID:SGTC_2357|Compound:9067248|FD-Score:4.28|P-value:5.76E-6 ID:SGTC_2358|Compound:9037157|FD-Score:3.18|P-value:5.37E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:3.12|P-value:6.49E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.10|P-value:7.06E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:-4.45|P-value:3.83E-6 ID:SGTC_2938|Compound:9059746|FD-Score:5.48|P-value:1.04E-8 ID:SGTC_2947|Compound:9072639|FD-Score:3.49|P-value:1.68E-4 ID:SGTC_2953|Compound:9074162|FD-Score:-3.24|P-value:5.88E-4 ID:SGTC_2997|Compound:9067075|FD-Score:3.55|P-value:1.30E-4 ID:SGTC_3038|Compound:9091290|FD-Score:3.69|P-value:7.40E-5 ID:SGTC_3157|Compound:9099274|FD-Score:3.64|P-value:9.20E-5 ID:SGTC_117|Compound:lucigenin|FD-Score:3.18|P-value:5.36E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:4.65|P-value:9.46E-7 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:3.34|P-value:2.95E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.55|P-value:2.41E-6 ID:SGTC_467|Compound:ro 31-8220|FD-Score:5.07|P-value:1.01E-7 ID:SGTC_611|Compound:3453-1652|FD-Score:3.24|P-value:4.23E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:4.59|P-value:1.25E-6 ID:SGTC_688|Compound:0527-0193|FD-Score:-3.18|P-value:7.21E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:3.58|P-value:1.17E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:4.14|P-value:1.08E-5 ID:SGTC_765|Compound:1319-0117|FD-Score:3.86|P-value:3.69E-5 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.78|P-value:5.17E-5 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.06|P-value:8.20E-4 ID:SGTC_1194|Compound:1488-0194|FD-Score:3.19|P-value:5.05E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:-7.72|P-value:3.24E-15 ID:SGTC_1365|Compound:1672-3266|FD-Score:3.98|P-value:2.17E-5 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.19|P-value:5.16E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.76|P-value:8.51E-7 ID:SGTC_1750|Compound:st081859|FD-Score:3.14|P-value:6.19E-4 ID:SGTC_1764|Compound:st044502|FD-Score:3.15|P-value:5.84E-4 ID:SGTC_1867|Compound:st057372|FD-Score:4.95|P-value:1.94E-7 ID:SGTC_1911|Compound:st060208|FD-Score:3.35|P-value:2.82E-4 ID:SGTC_1921|Compound:5740136|FD-Score:-3.43|P-value:2.96E-4 ID:SGTC_1968|Compound:st069590|FD-Score:3.09|P-value:7.31E-4 ID:SGTC_1975|Compound:st070967|FD-Score:-3.57|P-value:1.72E-4 ID:SGTC_1976|Compound:st070609|FD-Score:-4.27|P-value:8.86E-6 ID:SGTC_2012|Compound:4023501|FD-Score:3.14|P-value:6.08E-4 ID:SGTC_2038|Compound:4100038|FD-Score:3.80|P-value:4.81E-5 ID:SGTC_2205|Compound:7238770|FD-Score:-3.88|P-value:5.00E-5 ID:SGTC_2357|Compound:9067248|FD-Score:4.28|P-value:5.76E-6 ID:SGTC_2358|Compound:9037157|FD-Score:3.18|P-value:5.37E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:3.12|P-value:6.49E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.10|P-value:7.06E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:-4.45|P-value:3.83E-6 ID:SGTC_2938|Compound:9059746|FD-Score:5.48|P-value:1.04E-8 ID:SGTC_2947|Compound:9072639|FD-Score:3.49|P-value:1.68E-4 ID:SGTC_2953|Compound:9074162|FD-Score:-3.24|P-value:5.88E-4 ID:SGTC_2997|Compound:9067075|FD-Score:3.55|P-value:1.30E-4 ID:SGTC_3038|Compound:9091290|FD-Score:3.69|P-value:7.40E-5 ID:SGTC_3157|Compound:9099274|FD-Score:3.64|P-value:9.20E-5

Top fitness defect scores for YBR017C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2938 9059746 5.48 27.3 µM 1.04E-8 fatty acid desaturase (OLE1) 18 2.17E-8
2 SGTC_467 ro 31-8220 5.07 72.8 µM 1.01E-7 calcium & mitochondrial duress 45 1.95E-7
3 SGTC_1867 st057372 4.95 64.0 µM 1.94E-7 2 3.64E-7
4 SGTC_118 0083-0097 4.65 135.3 µM 9.46E-7 translation 76 1.67E-6
5 SGTC_625 k213-0058 4.59 121.0 µM 1.25E-6 translation 69 2.19E-6
6 SGTC_2357 9067248 4.28 200.0 µM 5.76E-6 11 9.51E-6
7 SGTC_710 3448-4187 4.14 58.0 µM 1.08E-5 fatty acid desaturase (OLE1) 54 1.74E-5
8 SGTC_1365 1672-3266 3.98 55.4 µM 2.17E-5 17 3.40E-5
9 SGTC_765 1319-0117 3.86 244.0 µM 3.69E-5 60S ribosome export 14 5.68E-5
10 SGTC_2038 4100038 3.80 31.0 µM 4.81E-5 plasma membrane duress 58 7.32E-5
11 SGTC_1032 k061-1018 3.78 98.7 µM 5.17E-5 27 7.85E-5
12 SGTC_3038 9091290 3.69 49.5 µM 7.40E-5 21 1.11E-4
13 SGTC_3157 9099274 3.64 49.5 µM 9.20E-5 26 1.37E-4
14 SGTC_704 1310-0270 3.58 142.0 µM 1.17E-4 45 1.73E-4
15 SGTC_2997 9067075 3.55 71.4 µM 1.30E-4 unfolded protein response 67 1.90E-4
16 SGTC_2947 9072639 3.49 7.7 µM 1.68E-4 tubulin folding & SWR complex 59 2.43E-4
17 SGTC_1911 st060208 3.35 74.3 µM 2.82E-4 55 4.01E-4
18 SGTC_241 5hr heat shock (37°C) + cantharidin 3.34 155.4 µM 2.95E-4 cell wall signaling 100 4.19E-4
19 SGTC_611 3453-1652 3.24 23.7 µM 4.23E-4 50 5.92E-4
20 SGTC_1194 1488-0194 3.19 225.0 µM 5.05E-4 60 7.02E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.146 2.03E-17 YHR010W RPL27A hom Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication
0.140 4.16E-16 YCR047C BUD23 hom Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern
0.123 8.04E-13 YPL239W YAR1 hom Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock
0.123 9.54E-13 YKR092C SRP40 hom Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140
0.120 3.34E-12 YEL044W IES6 hom Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes
0.118 5.97E-12 YDL081C RPP1A hom Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component
0.113 6.39E-11 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.108 3.52E-10 YLL027W ISA1 hom Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins
0.107 4.87E-10 YDL075W RPL31A hom Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication
0.105 9.91E-10 YOR309C_d YOR309C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58
0.105 1.02E-9 YGR162W TIF4631 hom Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication
0.105 1.20E-9 YGL246C RAI1 hom Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z
0.103 2.18E-9 YLR222C UTP13 het Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
0.102 3.69E-9 YGL148W ARO2 hom Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress
0.102 3.76E-9 YHR064C SSZ1 hom Hsp70 protein that interacts with Zuo1p (a DnaJ homolog) to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP