YBR023C / CHS3

Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR023C / CHS3

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:6.67|P-value:6.06E-11 ID:SGTC_104|Compound:0074-0074|FD-Score:-3.19|P-value:7.51E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:-3.19|P-value:7.50E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:15.80|P-value:1.37E-53 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.23|P-value:6.59E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:9.14|P-value:4.76E-19 ID:SGTC_255|Compound:tunicamycin|FD-Score:-4.87|P-value:7.70E-7 ID:SGTC_268|Compound:camptothecin|FD-Score:4.75|P-value:2.46E-6 ID:SGTC_304|Compound:1310-0298|FD-Score:3.84|P-value:1.17E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:6.45|P-value:2.50E-10 ID:SGTC_354|Compound:2783-3500|FD-Score:5.75|P-value:1.50E-8 ID:SGTC_360|Compound:0957-0399|FD-Score:3.48|P-value:4.43E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:9.10|P-value:6.84E-19 ID:SGTC_582|Compound:0987-0078|FD-Score:3.30|P-value:8.18E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:7.54|P-value:1.58E-13 ID:SGTC_796|Compound:1234-0020|FD-Score:4.27|P-value:2.05E-5 ID:SGTC_801|Compound:0866-0028|FD-Score:4.31|P-value:1.78E-5 ID:SGTC_808|Compound:0711-0032|FD-Score:3.43|P-value:5.22E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:11.30|P-value:5.68E-28 ID:SGTC_916|Compound:4466-0038|FD-Score:3.80|P-value:1.37E-4 ID:SGTC_928|Compound:2764-0428|FD-Score:6.14|P-value:1.65E-9 ID:SGTC_938|Compound:3126-1755|FD-Score:3.48|P-value:4.44E-4 ID:SGTC_950|Compound:1165-0508|FD-Score:-4.03|P-value:3.33E-5 ID:SGTC_951|Compound:1171-0579|FD-Score:10.00|P-value:1.21E-22 ID:SGTC_964|Compound:1287-0107|FD-Score:10.80|P-value:5.61E-26 ID:SGTC_972|Compound:1319-0219|FD-Score:3.62|P-value:2.69E-4 ID:SGTC_1037|Compound:k284-1091|FD-Score:3.58|P-value:3.11E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:3.79|P-value:1.42E-4 ID:SGTC_1206|Compound:3978-0017|FD-Score:4.57|P-value:5.77E-6 ID:SGTC_1277|Compound:0873-0036|FD-Score:3.37|P-value:6.34E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:5.98|P-value:4.08E-9 ID:SGTC_1367|Compound:1812-0510|FD-Score:3.55|P-value:3.40E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.31|P-value:7.72E-4 ID:SGTC_1664|Compound:st014217|FD-Score:3.51|P-value:3.88E-4 ID:SGTC_1687|Compound:st020778|FD-Score:4.93|P-value:1.06E-6 ID:SGTC_1699|Compound:st031318|FD-Score:4.04|P-value:5.47E-5 ID:SGTC_1743|Compound:st037606|FD-Score:4.43|P-value:1.05E-5 ID:SGTC_1745|Compound:st081495|FD-Score:3.41|P-value:5.53E-4 ID:SGTC_1760|Compound:st045453|FD-Score:3.77|P-value:1.55E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.53|P-value:3.72E-4 ID:SGTC_1780|Compound:st038448|FD-Score:-4.04|P-value:3.25E-5 ID:SGTC_1801|Compound:st050089|FD-Score:3.26|P-value:9.32E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:3.92|P-value:8.53E-5 ID:SGTC_1892|Compound:st058411|FD-Score:3.68|P-value:2.15E-4 ID:SGTC_1893|Compound:st059353|FD-Score:4.18|P-value:3.08E-5 ID:SGTC_2104|Compound:5389443|FD-Score:-3.17|P-value:8.17E-4 ID:SGTC_2138|Compound:5575593|FD-Score:3.27|P-value:8.93E-4 ID:SGTC_2181|Compound:6316240|FD-Score:3.66|P-value:2.31E-4 ID:SGTC_2210|Compound:7115885|FD-Score:7.03|P-value:5.74E-12 ID:SGTC_2292|Compound:7671323|FD-Score:4.54|P-value:6.39E-6 ID:SGTC_2361|Compound:9034121|FD-Score:3.42|P-value:5.38E-4 ID:SGTC_2400|Compound:5203494|FD-Score:4.96|P-value:9.01E-7 ID:SGTC_2444|Compound:5485484|FD-Score:4.31|P-value:1.78E-5 ID:SGTC_2479|Compound:5617353|FD-Score:4.01|P-value:6.12E-5 ID:SGTC_2495|Compound:cafestol|FD-Score:-4.93|P-value:5.65E-7 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:3.72|P-value:1.81E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.00|P-value:6.40E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:8.51|P-value:9.14E-17 ID:SGTC_2584|Compound:androsterone|FD-Score:14.00|P-value:1.70E-42 ID:SGTC_2619|Compound:obliquin|FD-Score:7.27|P-value:1.13E-12 ID:SGTC_2652|Compound:actinonin|FD-Score:4.85|P-value:1.57E-6 ID:SGTC_2756|Compound:carbadox|FD-Score:4.22|P-value:2.57E-5 ID:SGTC_2780|Compound:5215298|FD-Score:4.96|P-value:9.12E-7 ID:SGTC_2799|Compound:7812892|FD-Score:6.06|P-value:2.53E-9 ID:SGTC_2840|Compound:9008771|FD-Score:3.99|P-value:6.49E-5 ID:SGTC_2912|Compound:7964298|FD-Score:8.01|P-value:4.84E-15 ID:SGTC_2952|Compound:9073731|FD-Score:3.40|P-value:5.68E-4 ID:SGTC_2970|Compound:9090938|FD-Score:3.27|P-value:9.02E-4 ID:SGTC_3044|Compound:9093193|FD-Score:3.38|P-value:6.22E-4 ID:SGTC_3052|Compound:9092717|FD-Score:-3.84|P-value:7.08E-5 ID:SGTC_3057|Compound:9095946|FD-Score:-5.54|P-value:2.37E-8 ID:SGTC_3100|Compound:9120536|FD-Score:4.06|P-value:4.89E-5 ID:SGTC_3173|Compound:9105693|FD-Score:5.34|P-value:1.41E-7 ID:SGTC_3252|Compound:9135871|FD-Score:3.99|P-value:6.49E-5 ID:SGTC_3266|Compound:9136860|FD-Score:13.70|P-value:9.64E-41 ID:SGTC_3275|Compound:9138069|FD-Score:-3.19|P-value:7.55E-4 ID:SGTC_3299|Compound:9121982|FD-Score:3.39|P-value:5.97E-4 ID:SGTC_3311|Compound:9131146|FD-Score:8.32|P-value:4.55E-16 ID:SGTC_3321|Compound:9137793|FD-Score:4.51|P-value:7.48E-6 ID:SGTC_3322|Compound:9139058|FD-Score:4.28|P-value:1.96E-5 ID:SGTC_3350|Compound:9150499|FD-Score:5.26|P-value:2.06E-7 ID:SGTC_3351|Compound:9150312|FD-Score:5.61|P-value:3.25E-8 ID:SGTC_3356|Compound:9151811|FD-Score:5.64|P-value:2.85E-8 ID:SGTC_58|Compound:0957-0393|FD-Score:6.67|P-value:6.06E-11 ID:SGTC_104|Compound:0074-0074|FD-Score:-3.19|P-value:7.51E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:-3.19|P-value:7.50E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:15.80|P-value:1.37E-53 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.23|P-value:6.59E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:9.14|P-value:4.76E-19 ID:SGTC_255|Compound:tunicamycin|FD-Score:-4.87|P-value:7.70E-7 ID:SGTC_268|Compound:camptothecin|FD-Score:4.75|P-value:2.46E-6 ID:SGTC_304|Compound:1310-0298|FD-Score:3.84|P-value:1.17E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:6.45|P-value:2.50E-10 ID:SGTC_354|Compound:2783-3500|FD-Score:5.75|P-value:1.50E-8 ID:SGTC_360|Compound:0957-0399|FD-Score:3.48|P-value:4.43E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:9.10|P-value:6.84E-19 ID:SGTC_582|Compound:0987-0078|FD-Score:3.30|P-value:8.18E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:7.54|P-value:1.58E-13 ID:SGTC_796|Compound:1234-0020|FD-Score:4.27|P-value:2.05E-5 ID:SGTC_801|Compound:0866-0028|FD-Score:4.31|P-value:1.78E-5 ID:SGTC_808|Compound:0711-0032|FD-Score:3.43|P-value:5.22E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:11.30|P-value:5.68E-28 ID:SGTC_916|Compound:4466-0038|FD-Score:3.80|P-value:1.37E-4 ID:SGTC_928|Compound:2764-0428|FD-Score:6.14|P-value:1.65E-9 ID:SGTC_938|Compound:3126-1755|FD-Score:3.48|P-value:4.44E-4 ID:SGTC_950|Compound:1165-0508|FD-Score:-4.03|P-value:3.33E-5 ID:SGTC_951|Compound:1171-0579|FD-Score:10.00|P-value:1.21E-22 ID:SGTC_964|Compound:1287-0107|FD-Score:10.80|P-value:5.61E-26 ID:SGTC_972|Compound:1319-0219|FD-Score:3.62|P-value:2.69E-4 ID:SGTC_1037|Compound:k284-1091|FD-Score:3.58|P-value:3.11E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:3.79|P-value:1.42E-4 ID:SGTC_1206|Compound:3978-0017|FD-Score:4.57|P-value:5.77E-6 ID:SGTC_1277|Compound:0873-0036|FD-Score:3.37|P-value:6.34E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:5.98|P-value:4.08E-9 ID:SGTC_1367|Compound:1812-0510|FD-Score:3.55|P-value:3.40E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.31|P-value:7.72E-4 ID:SGTC_1664|Compound:st014217|FD-Score:3.51|P-value:3.88E-4 ID:SGTC_1687|Compound:st020778|FD-Score:4.93|P-value:1.06E-6 ID:SGTC_1699|Compound:st031318|FD-Score:4.04|P-value:5.47E-5 ID:SGTC_1743|Compound:st037606|FD-Score:4.43|P-value:1.05E-5 ID:SGTC_1745|Compound:st081495|FD-Score:3.41|P-value:5.53E-4 ID:SGTC_1760|Compound:st045453|FD-Score:3.77|P-value:1.55E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.53|P-value:3.72E-4 ID:SGTC_1780|Compound:st038448|FD-Score:-4.04|P-value:3.25E-5 ID:SGTC_1801|Compound:st050089|FD-Score:3.26|P-value:9.32E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:3.92|P-value:8.53E-5 ID:SGTC_1892|Compound:st058411|FD-Score:3.68|P-value:2.15E-4 ID:SGTC_1893|Compound:st059353|FD-Score:4.18|P-value:3.08E-5 ID:SGTC_2104|Compound:5389443|FD-Score:-3.17|P-value:8.17E-4 ID:SGTC_2138|Compound:5575593|FD-Score:3.27|P-value:8.93E-4 ID:SGTC_2181|Compound:6316240|FD-Score:3.66|P-value:2.31E-4 ID:SGTC_2210|Compound:7115885|FD-Score:7.03|P-value:5.74E-12 ID:SGTC_2292|Compound:7671323|FD-Score:4.54|P-value:6.39E-6 ID:SGTC_2361|Compound:9034121|FD-Score:3.42|P-value:5.38E-4 ID:SGTC_2400|Compound:5203494|FD-Score:4.96|P-value:9.01E-7 ID:SGTC_2444|Compound:5485484|FD-Score:4.31|P-value:1.78E-5 ID:SGTC_2479|Compound:5617353|FD-Score:4.01|P-value:6.12E-5 ID:SGTC_2495|Compound:cafestol|FD-Score:-4.93|P-value:5.65E-7 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:3.72|P-value:1.81E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.00|P-value:6.40E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:8.51|P-value:9.14E-17 ID:SGTC_2584|Compound:androsterone|FD-Score:14.00|P-value:1.70E-42 ID:SGTC_2619|Compound:obliquin|FD-Score:7.27|P-value:1.13E-12 ID:SGTC_2652|Compound:actinonin|FD-Score:4.85|P-value:1.57E-6 ID:SGTC_2756|Compound:carbadox|FD-Score:4.22|P-value:2.57E-5 ID:SGTC_2780|Compound:5215298|FD-Score:4.96|P-value:9.12E-7 ID:SGTC_2799|Compound:7812892|FD-Score:6.06|P-value:2.53E-9 ID:SGTC_2840|Compound:9008771|FD-Score:3.99|P-value:6.49E-5 ID:SGTC_2912|Compound:7964298|FD-Score:8.01|P-value:4.84E-15 ID:SGTC_2952|Compound:9073731|FD-Score:3.40|P-value:5.68E-4 ID:SGTC_2970|Compound:9090938|FD-Score:3.27|P-value:9.02E-4 ID:SGTC_3044|Compound:9093193|FD-Score:3.38|P-value:6.22E-4 ID:SGTC_3052|Compound:9092717|FD-Score:-3.84|P-value:7.08E-5 ID:SGTC_3057|Compound:9095946|FD-Score:-5.54|P-value:2.37E-8 ID:SGTC_3100|Compound:9120536|FD-Score:4.06|P-value:4.89E-5 ID:SGTC_3173|Compound:9105693|FD-Score:5.34|P-value:1.41E-7 ID:SGTC_3252|Compound:9135871|FD-Score:3.99|P-value:6.49E-5 ID:SGTC_3266|Compound:9136860|FD-Score:13.70|P-value:9.64E-41 ID:SGTC_3275|Compound:9138069|FD-Score:-3.19|P-value:7.55E-4 ID:SGTC_3299|Compound:9121982|FD-Score:3.39|P-value:5.97E-4 ID:SGTC_3311|Compound:9131146|FD-Score:8.32|P-value:4.55E-16 ID:SGTC_3321|Compound:9137793|FD-Score:4.51|P-value:7.48E-6 ID:SGTC_3322|Compound:9139058|FD-Score:4.28|P-value:1.96E-5 ID:SGTC_3350|Compound:9150499|FD-Score:5.26|P-value:2.06E-7 ID:SGTC_3351|Compound:9150312|FD-Score:5.61|P-value:3.25E-8 ID:SGTC_3356|Compound:9151811|FD-Score:5.64|P-value:2.85E-8

Top fitness defect scores for YBR023C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_138 0850-0259 15.80 40.9 µM 1.37E-53 cell wall 15 8.57E-57
2 SGTC_2584 androsterone 14.00 48.7 µM 1.70E-42 cell wall 8 4.65E-45
3 SGTC_3266 9136860 13.70 49.5 µM 9.64E-41 cell wall 2 3.34E-43
4 SGTC_863 0466-0191 11.30 129.0 µM 5.68E-28 cell wall 8 1.10E-29
5 SGTC_964 1287-0107 10.80 19.4 µM 5.61E-26 cell wall 5 1.43E-27
6 SGTC_951 1171-0579 10.00 82.7 µM 1.21E-22 cell wall 9 4.90E-24
7 SGTC_151 3013-0144 9.14 2.3 µM 4.76E-19 iron homeostasis 3 3.15E-20
8 SGTC_514 4466-0038 9.10 2.6 nM 6.84E-19 cell wall 28 4.64E-20
9 SGTC_2582 agaric acid 8.51 100.0 µM 9.14E-17 redox potentiating 96 8.34E-18
10 SGTC_3311 9131146 8.32 2.9 µM 4.55E-16 cell wall 28 4.57E-17
11 SGTC_2912 7964298 8.01 10.9 µM 4.84E-15 cell wall 25 5.62E-16
12 SGTC_586 0957-0265 7.54 8.2 µM 1.58E-13 cell wall 9 2.27E-14
13 SGTC_2619 obliquin 7.27 87.9 µM 1.13E-12 cell wall 54 1.84E-13
14 SGTC_2210 7115885 7.03 190.4 µM 5.74E-12 cell wall signaling 6 1.03E-12
15 SGTC_58 0957-0393 6.67 9.0 µM 6.06E-11 cell wall 177 1.26E-11
16 SGTC_351 caspofungin 6.45 25.0 nM 2.50E-10 cell wall 35 5.68E-11
17 SGTC_928 2764-0428 6.14 58.7 µM 1.65E-9 cell wall 13 4.23E-10
18 SGTC_2799 7812892 6.06 81.8 µM 2.53E-9 superoxide 10 6.64E-10
19 SGTC_1286 0929-0070 5.98 131.0 µM 4.08E-9 4 1.10E-9
20 SGTC_354 2783-3500 5.75 4.9 µM 1.50E-8 cell wall 20 4.41E-9

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.615 0 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.468 2.90E-182 YBL006C LDB7 hom Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
0.452 8.73E-169 YDL066W IDP1 hom Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
0.438 3.88E-157 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.438 4.73E-157 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.436 1.00E-155 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.426 7.69E-148 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.425 2.40E-147 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.423 1.43E-145 YBL061C SKT5 hom Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
0.419 1.31E-142 YPL115C BEM3 hom Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
0.407 5.16E-134 YPR165W RHO1 het GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
0.403 1.86E-131 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.398 8.52E-128 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.380 1.02E-115 YPL066W RGL1 hom Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
0.373 1.35E-111 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress