YBR028C / YPK3

An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR028C / YPK3

Click on Significant Values for Screen Details ID:SGTC_94|Compound:0334-0113|FD-Score:5.25|P-value:5.75E-7 ID:SGTC_274|Compound:sirtinol|FD-Score:-3.47|P-value:4.66E-4 ID:SGTC_306|Compound:1672-3436|FD-Score:-3.46|P-value:4.91E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:-3.81|P-value:1.45E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:-4.45|P-value:1.21E-5 ID:SGTC_401|Compound:neomycin|FD-Score:3.73|P-value:2.95E-4 ID:SGTC_553|Compound:k007-0256|FD-Score:3.57|P-value:5.05E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:5.00|P-value:1.80E-6 ID:SGTC_617|Compound:3770-0098|FD-Score:5.69|P-value:6.72E-8 ID:SGTC_696|Compound:1560-0013|FD-Score:3.58|P-value:4.77E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:3.72|P-value:2.98E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:3.66|P-value:3.73E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:-3.75|P-value:1.83E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:3.52|P-value:5.91E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:4.14|P-value:6.47E-5 ID:SGTC_1252|Compound:0493-0875|FD-Score:-3.64|P-value:2.67E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:3.68|P-value:3.46E-4 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:4.30|P-value:3.47E-5 ID:SGTC_1335|Compound:1486-0900|FD-Score:3.44|P-value:7.63E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:4.96|P-value:2.18E-6 ID:SGTC_1486|Compound:3970-0793|FD-Score:3.42|P-value:8.18E-4 ID:SGTC_1716|Compound:st033144|FD-Score:-3.64|P-value:2.65E-4 ID:SGTC_1719|Compound:st032227|FD-Score:6.53|P-value:6.77E-10 ID:SGTC_1721|Compound:st082152|FD-Score:3.37|P-value:9.58E-4 ID:SGTC_1857|Compound:st056007|FD-Score:3.51|P-value:6.02E-4 ID:SGTC_1958|Compound:st076513|FD-Score:3.98|P-value:1.18E-4 ID:SGTC_1962|Compound:st076595|FD-Score:3.73|P-value:2.87E-4 ID:SGTC_2037|Compound:5169046|FD-Score:3.53|P-value:5.65E-4 ID:SGTC_2040|Compound:5100729|FD-Score:5.05|P-value:1.45E-6 ID:SGTC_2097|Compound:5376186|FD-Score:3.73|P-value:2.86E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:5.41|P-value:2.68E-7 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:-3.33|P-value:7.43E-4 ID:SGTC_2423|Compound:st077119|FD-Score:3.37|P-value:9.68E-4 ID:SGTC_2451|Compound:5856223|FD-Score:3.42|P-value:8.25E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:3.53|P-value:5.72E-4 ID:SGTC_2698|Compound:st077697|FD-Score:4.99|P-value:1.88E-6 ID:SGTC_2703|Compound:st077852|FD-Score:3.89|P-value:1.65E-4 ID:SGTC_2715|Compound:st079500|FD-Score:3.93|P-value:1.42E-4 ID:SGTC_3009|Compound:9080819|FD-Score:5.95|P-value:1.71E-8 ID:SGTC_3028|Compound:9087778|FD-Score:4.42|P-value:2.21E-5 ID:SGTC_3100|Compound:9120536|FD-Score:4.30|P-value:3.50E-5 ID:SGTC_3122|Compound:9126309|FD-Score:3.96|P-value:1.26E-4 ID:SGTC_94|Compound:0334-0113|FD-Score:5.25|P-value:5.75E-7 ID:SGTC_274|Compound:sirtinol|FD-Score:-3.47|P-value:4.66E-4 ID:SGTC_306|Compound:1672-3436|FD-Score:-3.46|P-value:4.91E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:-3.81|P-value:1.45E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:-4.45|P-value:1.21E-5 ID:SGTC_401|Compound:neomycin|FD-Score:3.73|P-value:2.95E-4 ID:SGTC_553|Compound:k007-0256|FD-Score:3.57|P-value:5.05E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:5.00|P-value:1.80E-6 ID:SGTC_617|Compound:3770-0098|FD-Score:5.69|P-value:6.72E-8 ID:SGTC_696|Compound:1560-0013|FD-Score:3.58|P-value:4.77E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:3.72|P-value:2.98E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:3.66|P-value:3.73E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:-3.75|P-value:1.83E-4 ID:SGTC_1126|Compound:isoteolin|FD-Score:3.52|P-value:5.91E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:4.14|P-value:6.47E-5 ID:SGTC_1252|Compound:0493-0875|FD-Score:-3.64|P-value:2.67E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:3.68|P-value:3.46E-4 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:4.30|P-value:3.47E-5 ID:SGTC_1335|Compound:1486-0900|FD-Score:3.44|P-value:7.63E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:4.96|P-value:2.18E-6 ID:SGTC_1486|Compound:3970-0793|FD-Score:3.42|P-value:8.18E-4 ID:SGTC_1716|Compound:st033144|FD-Score:-3.64|P-value:2.65E-4 ID:SGTC_1719|Compound:st032227|FD-Score:6.53|P-value:6.77E-10 ID:SGTC_1721|Compound:st082152|FD-Score:3.37|P-value:9.58E-4 ID:SGTC_1857|Compound:st056007|FD-Score:3.51|P-value:6.02E-4 ID:SGTC_1958|Compound:st076513|FD-Score:3.98|P-value:1.18E-4 ID:SGTC_1962|Compound:st076595|FD-Score:3.73|P-value:2.87E-4 ID:SGTC_2037|Compound:5169046|FD-Score:3.53|P-value:5.65E-4 ID:SGTC_2040|Compound:5100729|FD-Score:5.05|P-value:1.45E-6 ID:SGTC_2097|Compound:5376186|FD-Score:3.73|P-value:2.86E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:5.41|P-value:2.68E-7 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:-3.33|P-value:7.43E-4 ID:SGTC_2423|Compound:st077119|FD-Score:3.37|P-value:9.68E-4 ID:SGTC_2451|Compound:5856223|FD-Score:3.42|P-value:8.25E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:3.53|P-value:5.72E-4 ID:SGTC_2698|Compound:st077697|FD-Score:4.99|P-value:1.88E-6 ID:SGTC_2703|Compound:st077852|FD-Score:3.89|P-value:1.65E-4 ID:SGTC_2715|Compound:st079500|FD-Score:3.93|P-value:1.42E-4 ID:SGTC_3009|Compound:9080819|FD-Score:5.95|P-value:1.71E-8 ID:SGTC_3028|Compound:9087778|FD-Score:4.42|P-value:2.21E-5 ID:SGTC_3100|Compound:9120536|FD-Score:4.30|P-value:3.50E-5 ID:SGTC_3122|Compound:9126309|FD-Score:3.96|P-value:1.26E-4

Top fitness defect scores for YBR028C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1719 st032227 6.53 49.2 µM 6.77E-10 3 3.35E-11
2 SGTC_3009 9080819 5.95 71.4 µM 1.71E-8 4 1.36E-9
3 SGTC_617 3770-0098 5.69 35.0 µM 6.72E-8 exosome 5 6.51E-9
4 SGTC_2250 alverine citrate 5.41 130.0 µM 2.68E-7 77 3.18E-8
5 SGTC_94 0334-0113 5.25 514.8 µM 5.75E-7 7 7.63E-8
6 SGTC_2040 5100729 5.05 123.0 µM 1.45E-6 18 2.21E-7
7 SGTC_557 1315-0110 5.00 16.2 µM 1.80E-6 Golgi 4 2.82E-7
8 SGTC_2698 st077697 4.99 67.5 µM 1.88E-6 8 2.96E-7
9 SGTC_1447 4239-0816 4.96 81.9 µM 2.18E-6 12 3.51E-7
10 SGTC_3028 9087778 4.42 49.5 µM 2.21E-5 8 5.00E-6
11 SGTC_1292 0kpi-0043 4.30 232.0 µM 3.47E-5 8 8.38E-6
12 SGTC_3100 9120536 4.30 49.5 µM 3.50E-5 6 8.45E-6
13 SGTC_1248 0469-0687 4.14 58.6 µM 6.47E-5 23 1.71E-5
14 SGTC_1958 st076513 3.98 41.9 µM 1.18E-4 60S ribosome export 28 3.42E-5
15 SGTC_3122 9126309 3.96 49.5 µM 1.26E-4 cell wall signaling 8 3.68E-5
16 SGTC_2715 st079500 3.93 56.4 µM 1.42E-4 8 4.21E-5
17 SGTC_2703 st077852 3.89 53.4 µM 1.65E-4 26 5.00E-5
18 SGTC_2097 5376186 3.73 200.0 µM 2.86E-4 28 9.41E-5
19 SGTC_1962 st076595 3.73 64.5 µM 2.87E-4 redox potentiating 31 9.42E-5
20 SGTC_401 neomycin 3.73 50.0 µM 2.95E-4 14 9.75E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.147 1.52E-17 YPR006C ICL2 hom 2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol
0.119 5.45E-12 YPR009W SUT2 hom Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p
0.102 3.36E-9 YFR048W RMD8 hom Cytosolic protein required for sporulation
0.098 1.43E-8 YBL004W UTP20 het Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
0.088 3.83E-7 YLR438W CAR2 hom L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress
0.078 5.42E-6 YJL001W PRE3 het Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
0.078 5.94E-6 YGR286C BIO2 hom Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant
0.077 7.75E-6 YBR032W_d YBR032W_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data
0.073 2.18E-5 YDR381W YRA1 het RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member
0.073 2.41E-5 YMR306C-A_d YMR306C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.073 2.52E-5 YJL112W MDV1 hom Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication
0.073 2.57E-5 YDR213W UPC2 hom Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication
0.073 2.63E-5 YGR158C MTR3 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
0.072 2.75E-5 YIR022W SEC11 het 18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum
0.072 3.06E-5 YGL099W LSG1 het Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm